Objective To explore the clinical significance of interleukin (IL)- 17 in nasopharyngeal secretions (NPS) and serum of wheezing children under 5 years old. Methods Fifty-three children with recurrent wheezing under 5 years old were divided into wheezing group Ⅰ (with atopic high risks,27 cases)and wheezing group Ⅱ (without atopic high risks, 26 cases) ;20 children without infectious diseases such as hernia and renal calculus were enrolled as control group. After collecting and dealing their NPS and serum,the levels of IL-17 were measured by double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Results The levels of IL-17 in serum and NPS in wheezing group Ⅰ [(1469.32 ± 978.30),(1473.70 ±974.35) ng/L],and wheezing group Ⅱ [(263.34 ± 131.80), (788.28±132.40) ng/L] were significantly higher than those in control group [(36.48 ± 2.00), (36.45 ± 5.00) ng/L] (P ＜ 0.01 ), and the levels of IL- 17 in wheezing group Ⅰ were higher than those in wheezing group Ⅱ (P ＜ 0.05 ); the expression of IL- 17 in NPS and serum had a positive correlation (r = 0.313,P ＜ 0.05). Conclusion The detections of IL-17 in NPS can be used as laboratory index to distinguish wheezing children who have a tendency to persistent wheezing, and early intervention and treatment should be given.

Objective To investigate the clinical significance and prognostic value of PKD3 expression in human hepatocellu-lar carcinoma (HCC)after hepatectomy.Methods We analyzed mRNA expression of L1CAM in 1 10 HCCs by quantitative real-time PCR (qRT-PCR)and western blot,and the relationship among the overall survival of HCCs.Results The relative protein and mRNA expression level of L1CAM was up-regulated in HCCs comparing with adjacent non tumor liver tissues (P <0.01).L1CAM expression in the well-differentiated group was higher than that in the poor-differentiated group (P < 0.01 ).The expression of L1CAM mRNA was significantly correlated with tumor differentiation and TNM stage (P <0.05).The prognosis of patients with high expression L1CAM was poor (P <0.01).Conclusion L1CAM expression is related to occurrence and development of HCCs and may predict the prognosis of HCCs after hepatectomy.

Objective To explore the value of improved family management model to reduce the occurrence of death and rehospitalization rate of children with bronchopulmonary dysplasia (BPD) after discharge.Methods The children with BPD who were admitted in Neonatal Intensive Care Unit of Zhongshan Hospital Affiliated to Sun Yat-Sen University from January 2006 to December 2013 were enrolled.Thirty-one cases with BPD used with improved family management mode after discharge from January 2010 to December 2013 were as experimental group.Twenty-seven cases with BPD used with traditional family management mode after discharge from January 2006 to December 2009 were as control group.The occurrence of death and rehospitalization were observed because of apnea,choking,respiratory failure,pneumonia and asthma within 3 months after discharge and compared the difference between two groups with chi-square test.The lung functions of all cases were checked in 40 week of the corrected gestational age and 3 month of the corrected age and compare the difference between the experimental group and control group with t test.Results The occurrence of death in the experimental group was 3.22％ (1/31 cases),and that of the control group was 22.22％ (6/27 cases),there was significantly statistical difference in the occurrence of death between two groups (x2 =4.907,P ＜0.05).The occurrence of rehospitalization in the experimental group was 25.80％ (8/31 cases),and that of the control group was 77.78％ (21/27 eases),there was significantly statistical difference in the occurrence of rehospitalization because of apnea,choking,respiratory failure,pneumonia and asthma between two groups (x2 =15.591,P ＜ 0.05).The difference of the lung functions between two groups in 40 week of the corrected gestational age was not statistically significant.The respiratory rate in the experimental group (30 survivors) were lower than those in the control group (21 survivors) in 3 month of the corrected gestational age (t =9.225,P ＜ 0.05),and time to peak tidal expiratory flow,volume to peak tidal expiratory flow,25 ％ tidal expiratory flow,50％ tidal expiratory flow,75 ％ tidal expiratory flow levels in the experimental group were higher than those in the control group in 3 month of the corrected gestational age,the difference of lung functions between 2 groups were statistically significant (t =3.992,3.301,4.645,3.152,2.916,all P ＜ 0.05),the lung functions in experimental group were significantly better than that in control group in 3 month of the corrected age.Conclusions The improved family management mode can reduce the occurrence of death and rehospitalization for the children with BPD after discharge and is conducive to the improvenent of pulmonary function.

Objective To investigate the clinical application and value of retrograde catheterization via superficial femoral artery for the treatment of limb chronic total occlusive disease (CTO). Methods Fifty-nine patients with proximal and middle occlusive lesions of superficial femoral artery from Jan. 2013 to Jan. 2015 in department of emergency surgery were retrospectively analyzed. Percutaneous transluminal angioplasty (PTA) together with stent implantation was performed to reopen the all narrowed or obstructed superficial femoral artery.Seventeen patients received ipsilateral retrograde catheterization via superficial femoral artery together. The length of the diseased artery ranged from 6.5 to 13.4 cm, with a mean of (8.3 ± 2.9) cm, including right (11 cases)and left (8 cases) femoral arteries. After the interventional therapy patients were followed up for 1- 24 months. Results All patients were treated successfully. The proximal and middle occlusive lesions of superficial femoral artery were reopened.No serious complications were found including nerve and vessel damage+After the treatment, the ischemic symptoms were markedly improved or even disappeared. In 14/19 of patients the superficial femoral arteries remained patent during the follow-up period. The ankle preoperative brachial index (ABI) was 0.20 to 0.51 (0.39 ± 0.06), while the postoperative ABI was 0.65 to 1.15 (0.74 ± 0.13). The difference in ABI between pre-operation and post-operation was statistically significant (P<0.05). Conclusions Retrograde catheterization via superficial femoral artery is an effective alternative to treat proximal and middle occlusion of ipsilateral superficial femoral artery. It has high successful rate and safety.

Objective To study the expression and regulation of aquapofins (AQP) in cystic epithelial cells of jck mice with polycystic kidney disease. Methods Localization and regulation of AQP1, AQP2, AQP3 and AQP4 protein were analyzed by using the immunofluorescence and Western blotting. Results Kidneys of jck homozygous mice were 4 folds larger than those of litter matched wild-type mice. There were multiple cysts and fibrosis in the renal tissue of jck mice. The epithelial cells in cysts were flat in shape. Blood urea level in jck mice was (42.6 ± 6.7) mmol/L, which was 5 folds higher than that in wild-type mice [(8.4±1.9) mmol/L] (P<0.01). Immunofluorescence analysis showed that AQP1 was expressed in the apical and hasolatend membranes of epithelial cells in proximal tubules, as well as in the thin descending limb of Henle and endothelial cells of descending vasa recta. There was no AQP1 expression in epithelial cells of cysts. AQP2 was expressed in the apical membranes of collecting ducts and renal cysts. AQP3 and AQP4 were expressed in basolateral membranes of collecting duct and renal cystic epithelial cells of jck mice. Western blot analysis showed the same protein sizes of AQP1, AQP2, AQP3 and AQP4 in both jck and wild-type kidneys. However, AQP1 expression was down-regulated in jck kidneys (P<0.01). Conclusion The renal cystic epithelia expresses AQP2, AQP3 and AQP4, which indicates that epithelial cells in renal cysts are derived from renal collecting ducts in jck mice and aquaporins may play an important role in renal cyst development.

Objective To study the expression of ILD patients and health men during exercise,furthermore provide the reference of application of exercise test in ILD.Methods Cycle exercise test was used in 36 patients with ILD and 20 normal subjects,The oxygen uptake per minute(VO2),Borg Scale(BS),SpO2,and other index of exercise test were detected.Results Compared with normal subjects,The VO2max in ILD patients was decrease markedly;The BS was increased,SpO2 decreased markedly during exercise.Conclusions The results indicate that the hypoxemia and dyspnea are the limited factors of exercise in the patients with ILD;The capacity of exercise in ILD decrease compare with normal subjects.

Objective To study the expression of dyspnea and SpO2 with the patients with COPD and silicosis during exercise, further more to provide the reference in clinic treatment.Methods Cycle exercise test was used in 20 patients with COPD and 20 silicosis patients , The oxygen uptake per minute (VO2) ,Borg Scale (BS), SpO2, and other index of exercise test were detected. Results Dyspnea index as Borg scale was closely correlated with oxygen uptake (VO2) during exercise in all the patients. Borg scale slope (BSS) for quantitative evaluation of dyspnea could be derived from the linear regression line,and the BSS in COPD patients was higher than BSS in silicosis patients[(10.9?3.8)BS/(L?min) vs (7.2 ?1.9)BS/(L?min),P

Objective To explore the pathogenesis of syringoma in order to cure this disease. Methods The clinical manifestation was observed in 39 cases of syringoma. The serum level of estradiol (E 2), progesterone (Pro), luteinizing hormone(LH) and follicle stimulating hormone (FSH) were detected with radioimmunoassay in all the 30 female cases and 25 normal controls. The expression of estrogen and progesterone receptor (ER and PR) was determined in syringoma tissues using ABC method and anti ER and anti PR monoclonal antibodies. Results Syringoma mainly involved middle aged women. The average level of serum E 2 in female patients was (73?28) ng/ml and its control group, (42?19) ng/ml ( P

Objective To observe the renoprotective effects of angiotensin Ⅱ (ATⅡ) receptor antagonist, L-158809 and to explore its potential mechanisms. Methods The experimental rats consisted of normal control and type 2 diabetic model groups with or without treatment of L-158809 (2 mg?kg -1 ?d -1 ) for 16 weeks. Blood glucose, HbA 1c , serum immunoreactive insulin, serum creatinine, mean arterial blood pressure, creatinine clearance (Ccr) and urinary albumin excretion index (UAEI) as well as plasma and renal ATⅡ content were measured. The kidney morphological changes were examined by renal histopathology. Marix metalloproteinase-2 (MMP-2) and the tissue inhibitor of metalloproteinase-2 (TIMP-2) expression in renal tissues were studied by immunohistochemistry, zymography and Western blot. Results In type 2 diabetic rats, L-158809 restored the blood pressure (P

Objective To investigate the awareness of knowledge,attitude and behavior about disinfection and sterilization among clinical health care workers (HCWs).Methods 200 clinical HCWs were investigated randomly by using questionnaires.Results The average awareness rates of knowledge,attitude and behavior about disinfec-tion and sterilization among HCWs were 71 .49%,63.79%,and 86.45% respectively.The awareness rates of knowledge about the expiration dates of sterilized articles,classification of infectious diseases,and biological moni-toring were 49.43%,42.53%,and 23.56%,respectively;HCWs’attitude towards disinfection and sterilization:awareness rate of whether they paid attention to the new technology in specialty of central sterile supply was the lowest (26.44%);86.45% of HCWs thought they could master the aseptic operation technique,correct applica-tion method for sterile apparatus and related items,but easily ignored to check the integrity of aseptic packages and indicators before operation (67.24%).Conclusion The knowledge,attitude and behavior about disinfection and sterilization among HCWs still need to be improved,training on knowledge about disinfection and sterilization among HCWs should be strengthened.