AIM: To study the protetive effects of Danshenhonghua Injection (Radix Salviae Miltiorrhizae,Stigma Croci,etc) on acute cerebral ischemia in rats. METHODS : The model of acute incomplete cerebral ischemia was built by ligaturing bilateral carotid arteries. Cerebral index,cerebral water content ,capillary permeability of brain,and the change of cerebral morphology in rats were observed. RESULTS : The cerebral index,cerebral water content,capillary permeability of brain decreased remarkably with 7.2,14.4mg?kg -1 of Danshenhonghua Injection,and the injury of brain tissue was also abated by Danshenhonghua Injection significantly. CONCLUSION : Danshenhonghua Injection has the protetive effects on acute cerebral ischemia.

Objective To analyze risk factors for metabolic syndrome in Mongolia versus Han nationalities in Xilinhaote city. Methods Using the epidemiology investigation data of health examination,we calculated the prevalence of metabolic syndrome of Mongolia and Han nationalities, then used logistic regression model to explore risk factors of two nationalities. Results The crude prevalence of MS in Mongolia and Han nationality was 34.3%and 24.6% respectively. The multivariate logistic regression showed that male, the meat-rich diets and aging(OR:2.18, 1.92, 1.04 respectively)were the risk factors for Mongolia nationality, and smoking, family history of hypertension, drinking, the meat-rich diets, aging(OR:1.89, 1.84, 1.72, 1.61 and 1.04 respectively)were the risk factors for Han nationality. Conclusions Xinlinhaote population has higher MS prevalence, and different nationalities have different risk factors. We should take preventive actions to control it.

Object:To explore the effects of shared decision making-based intervention on decisional conflict and health literacy in stable coronary artery disease patients.Methods:A total of 86 stable coronary artery disease patients were divided into experimental group and control group according to the admission order, with 43 cases in each group. The patients in the control group recieved routine nursing, basis on this, the experimental group carried out shared decision making program. Before and after 1 month of intervention, the effects was assessed by Decisional conflict scale (DCS) and Health literacy management scale (HeLMS), respectively.Results:Before intervention, there was no significant difference in DCS and HeLMS scores between two group( P>0.05). After intervention, the information & values, decision uncertainty and total DCS scores were (6.95±2.13) pionts, (3.44±1.18) pionts and (20.95±3.99) pionts, significantly lower than in the control group (8.77±2.33) pionts, (4.95±1.46) pionts, (25.56±4.03) pionts; the information acquisition ability, interaction ability, willingness to improve health, willingness of financial support and total HeLMS scores were (36.77±4.85) pionts, (35.74±4.58) pionts, (15.07±1.55) pionts, (6.53±1.76) pionts, (94.12±6.44) pionts, significantly higher than in the control group [(34.37±5.84) pionts, (33.19±5.90) pionts, (14.23±1.43) pionts, (5.81±1.29) pionts, (87.60±9.18) pionts], the difference was statistically significant ( t values were 2.070-5.321, P<0.05). Conclusion:Shared decision making can effectively alleviate decisional conflict and improve health literacy of patients with stable coronary artery disease.

Objective:To construct a predictive model based on acute skin failure, and to evaluate its predictive value on the 28-day prognosis of patients with sepsis, to provide a basis for medical staff to develop effective intervention measures.Methods:A prospective survey method was adopted, 231 patients with sepsis hospitalized in Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine from May 2020 to April 2023 were enrolled as the research subjects, of which 162 patients from May 2020 to March 2022 were allocated into the test group for construct a prediction model, and 69 patients from April 2022 to April 2023 in the validation group for external validation. Univariate and multivariate Logistic regression were implemented to analyze the risk factors of 28-day mortality in sepsis patients, construction of a joint prediction model based on acute skin failure, and drawing of a column chart to verify its accuracy.Results:The 53 of 162 cases in the test group died, with mortality rate of 32.7%. The 19 of 69 cases in the validation group died, with mortality rate of 27.5%, there was no statistically significant difference in mortality rates between the two groups ( χ2 = 0.61, P = 0.437). The results of multivariate analysis in the test group showed that APACHE II score ( OR = 0.674, 95% CI 0.509-0.631), Sequential Organ Failure Assessment ( OR = 0.391, 95% CI 0.242-0.631), lactate ( OR = 2.291, 95% CI 1.306-4.019), skin mottling score ( OR = 2.950, 95% CI 1.586-5.488), skin wet cold ( OR = 3.678, 95% CI 0.910-1.865), capillary filling time>2 s ( OR = 6.070, 95% CI 0.774-1.579), decreased fingertip transcutaneous oxygen saturation ( OR = 2.046, 95% CI 1.312-2.076), and weakened skin sensation ( OR = 3.354, 95% CI 0.796-1.124) were independent risk factors that affecting the 28-day mortality of patients with sepsis. The verification results of combined predictive model for acute skin failure showed that the C-index of test group and validation group were 0.834 and 0.811 respectively; the areas under ROC curve were 0.834 and 0.807, respectively. Conclusions:Acute skin failure-based nomogram model can predict the 28-day mortality of patients with sepsis, and help medical staff to implement personalized intervention measures.

Objective To investigate the protective role of Sestrin2 overexpression in hypoxia and re-oxygenation injury (H/R) injury of hippocampal neurons and its mechanism in rats.Methods Neurons were enzymatically isolated from hippocampi of newborn Sprague-Dawley rats (less than 24 h old) and culturedinvitro. These neurons were randomly assigned into 4 groups (n=20) using a random number table: control group, H/R group, vector group and Sestrin2 overexpression group. The hippocampal neurons were seeded in 6-well plates at a density of 2×104 cells/mL; neurons in the latter two groups were transfected with lentiviruses containing empty vector andSestrin2overexpressed genes, respectively; the hippocampal neurons in the later three groups were subjected to oxygen-glucose deprivation (OGD) for 6 h followed by restoration of O2 supply for 20 h. The reactive oxygen species (ROS) content was detected by Reactive Species Assay Kit, and the ATP concentration was detected by ATP Assay Kit. Cell apoptosis rates were measured by flow cytometry. The levels of Sestrin2, dynamin-related protein 1 (Drp1), Fis1, and apoptosis-related proteins Bcl-2, Bax and cytochrome C (Cyt C) were measured by Western blotting. The ratio of Bcl-2 to Bax was calculated. The ultrastructure of mitochondria was observed by transmission electron microscopy.Results As compared with control group, H/R group had significantly lower ATP concentration, Bcl-2 protein expression and ratio of Bcl-2 to Bax ([11.15±0.42] nmol/mg proteinvs. [5.30±0.39] nmol/mg protein; 2.20±0.26vs. 0.91±0.02; 6.46± 0.41vs. 1.04±0.05), statistically higher average fluorescence intensity of ROS and cell apoptosis rate (152.41±17.38vs. 1530.00±14.69; 3.77％±0.74％vs. 56.57％±1.35％), and significantly higher protein levels of Sestrin2, Drp1, Fis1, Bax and Cyt C (0.66±0.06vs. 1.11±0.20; 0.48±0.03vs. 1.16±0.07; 1.14± 0.09vs. 2.47±0.09; 0.34±0.03vs. 0.88±0.04; 0.17±0.03vs. 0.30±0.03,P<0.05); what's more, the structure of mitochondria was obviously destroyed in I/R group. As compared with H/R group, Sestrin2 overexpression group had significantly increased ATP concentration, Sestrin2 and Bcl-2 protein expressions and ratio of Bcl-2 to Bax ([8.95±0.27] nmol/mg protein; 2.67±0.07; 1.80±0.19; 3.95±0.28), significantly lower average fluorescence intensity of ROS and cell apoptosis rate (337.27±15.32; 10.33％±2.60％), and statistically lower protein levels of Drp1, Fis1, Bax and Cyt C (0.43±0.02; 1.11±0.08; 0.45± 0.02; 0.17±0.02,P<0.05); the structure of mitochondria was relatively completed in Sestrin2 overexpression group.Conclusion Sestrin2 overexpression can inhibit mitochondrial fission, reduce accumulation of reactive oxygen species, improve mitochondrial energy metabolism and block mitochondria mediated apoptosis pathway, thereby alleviating I/R injury of rat hippocampal neurons.

Objective To explore the effect of evidence-based nursing on toxicity and side effects of breast cancer patients undergoing chemotherapy. Methods Forty-five patients with breast cancer treated in our department from January to March 2018 were selected as control group, receiving routine nursing. Forty-five patients with breast cancer treated by chemotherapy in our department from April to June 2018 were selected as observation group, given evidence-based nursing to prevent side effects on thebasis of routine nursing. The mastery of health knowledge of chemotherapy and related toxicity and side effects were compared between the two groups. Results The scores of health knowledge in basic knowledge of chemotherapy, bone marrow suppression, digestive tract and dietary nutrition in the observation group were higher than that in the control group (P ＜ 0. 05). The incidence of gastrointestinal adverse reactions, bone marrow suppression and stomatitis in the observation group was lower than that in the control group (P ＜ 0. 05). The compliance of the observation group was higher than that of the control group (P ＜ 0. 05). Conclusion Evidence-based nursing can increase the health knowledge of breast cancer patients undergoing chemotherapy, reduce toxicity and side effects, and improve their compliance with chemotherapy.

Objective To explore the effect of evidence-based nursing on toxicity and side effects of breast cancer patients undergoing chemotherapy. Methods Forty-five patients with breast cancer treated in our department from January to March 2018 were selected as control group, receiving routine nursing. Forty-five patients with breast cancer treated by chemotherapy in our department from April to June 2018 were selected as observation group, given evidence-based nursing to prevent side effects on thebasis of routine nursing. The mastery of health knowledge of chemotherapy and related toxicity and side effects were compared between the two groups. Results The scores of health knowledge in basic knowledge of chemotherapy, bone marrow suppression, digestive tract and dietary nutrition in the observation group were higher than that in the control group (P ＜ 0. 05). The incidence of gastrointestinal adverse reactions, bone marrow suppression and stomatitis in the observation group was lower than that in the control group (P ＜ 0. 05). The compliance of the observation group was higher than that of the control group (P ＜ 0. 05). Conclusion Evidence-based nursing can increase the health knowledge of breast cancer patients undergoing chemotherapy, reduce toxicity and side effects, and improve their compliance with chemotherapy.

Objective To investigate the role and mechanism of silent information regulator 1(SIRT1)-NOD-like receptor protein 3(NLRP3)axis in the effect of remifentanil against ischemia-reperfusion injury(IRI)in rat livers.SD rats were randomly divided into sham operation group(sham group),IRI group,IRI+remifentanil pretreatment group(IRI+RPC group),IRI+SIRT1 inhibitor EX-527 group(IRI+EX-527 group)and IRI+RPC+EX-527 group,with 8 rats in each group.The levels of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),lactate dehydrogenase(LDH),interleukin(IL)-1β and IL-18 of rats in each group were detected.The liver tissue pathology was observed.The apoptosis rate of hepatocytes in rats was detected.The expressions of SIRT1,NLRP3,cleaved cysteinyl aspartate specific proteinase-1(Cleaved Caspase-1)and Gasdermin D(GSDMD)proteins in rat liver tissue were detected.Results Compared with the sham group,the liver tissue pathological score and hepatocyte apoptosis rate of rats in the IRI group were increased,the serum ALT,AST,LDH,IL-1β,and IL-18 levels were increased,the relative expression of SIRT1 protein in liver tissue was decreased,and the relative expression of NLRP3,Cleaved Caspase-1,and GSDMD proteins were increased(all P<0.05).Compared with the IRI group,the liver tissue pathological score and hepatocyte apoptosis rate of rats in the IRI+RPC group were decreased,the serum ALT,AST,LDH,IL-1β,and IL-18 levels were decreased,the relative expression of SIRT1 protein in liver tissue was increased,and the relative expression of NLRP3,Cleaved Caspase-1,and GSDMD proteins were decreased;the liver tissue pathological score and hepatocyte apoptosis rate of rats in the IRI+EX-527 group were increased,the ALT,AST,LDH,IL-1β,and IL-18 levels were increased,the relative expression of SIRT1 protein in liver tissue was decreased,and the relative expression of NLRP3,Cleaved Caspase-1,and GSDMD proteins were increased(all P<0.05).Compared with the IRI+RPC group,the liver tissue pathological score and hepatocyte apoptosis rate in the IRI+RPC+EX-527 group were increased,the levels of ALT,AST,LDH,IL-1β,and IL-18 were increased,the relative expression of SIRT1 protein in liver tissue was decreased,and the relative expression of NLRP3,Cleaved Caspase-1,and GSDMD proteins were increased(all P<0.05).Conclusions SIRT1 may participate in the regulation of remifentanil against rat liver IRI by inhibiting NLRP3 mediated cell pyroptosis.

Objective To explore the effect of applying syndrome differentiation and medicated diet in patients with liver cirrhosis as cites based on"Gu Ben Kai Qu"theory of TCM. Methods Seventy patients of liver cirrhosis as cites were randomly divided into observation group (35 cases) and control group(35 cases).The control group was cared with care routine of liver cirrhosis as cites,The observation group was based on care routine traditional nursing and implementation of dialectical medicalated meal which based on "Gu Ben Kai Qu" theory of TCM. The two groups of patients were used to assess the efficacy of liver cirrhosis as cites,urine volume,abdominal circumference,weight,TCM syndrome scale of liver disease. Results The urine volume on the 7th day, the 14th day, 2 weeks after discharge,the weight and the abdominal circumference on the 2 weeks after discharge from the patients in the observation group were respectively(1.90±0.52)L,(2.09±0.56)L,(2.02±0.35)L,(59.36±8.19)kg,(89.56±7.24)cm,while they were(1.63±0.36)L,(1.79±0.40)L,(1.82±0.38)L,(63.33±8.21)kg,(93.57±7.56)cm in the control group,the difference between the two groups was statistically significant (F=6.083, 6.484, 5.030, 4.108, 5.144,all P<0.05). The efficacy of liver cirrhosis as cites from the patients in the observation group were cured 40.0%(14/35),improved 42.9%(15/35),not cured 17.1%(6/35),while they were cured 22.9%(8/35),improved 40.0%(14/35),not cured 37.1%(13/35),the difference between the two groups was statistically significant (Z=2.012,P<0.05). The efficacy of TCM Syndromes on liver disease from the patients in the observation group were respectively[significant effect 37.1%(13/35),valid 45.7% (16/35),invalid 17.2%(6/35)],[significant effect 60.0%(21/35),valid 28.6%(10/35),invalid 11.4%(4/35)], while they were respectively [significant effect 20.0%(7/35), valid 42.9%(15/35), invalid 37.1%(13/35)], [significant effect 40.0%(14/35),valid 25.7%(9/35),invalid 34.3%(12/35)],the difference between the two groups was statistically significant(Z=2.065,2.104,all P<0.05). Conclusions Based on"Gu Ben Kai Qu"theory of TCM,the implementation of dialectical diet can effectively improve efficacy of liver cirrhosis as cites, reduction rate of TCM symptoms scale, urine volume,reduce the weight,shrink down the abdominal circumference,worthy of clinical application.