[Objective]To reconstruct anterior cruciate ligament (ACL) by using ligament advanced reinforcement system(LARS),and to investigate the integration of LARS artificial ligament with bone interface in the animals with regard to imageology, biomechanics and histology.[Method]Twenty-four Boer goats were randomly divided into three groups. The animal mode of ACL reconstruction was established by clinical ACL reconstruction system. Gross observation was made, and histological, imageological and biomechanical changes were observed at 4, 8 and 12 weeks after surgery, respectively. Statistical analysis was performed.[Result](1)At 4 weeks after surgery,ligament-bone interface had great amount of loose bindweb and infiltration of chronic inflammation cells. At 8 weeks after surgery, there was new bone formation. Part of samples had Sharpey fibers. At 12 weeks after surgery, Sharpey fibers.and a large number of fibroblasts were noted in the interface between LARS artificial ligament and bone interface. But calcified cartilage was not founded.(2)The imageology examination for group 3 was made at 4, 8 and 12 weeks after surgery. The data were analyzed statistically by the image processing software of eflime,and there was evident statistical difference (P0.05 ) .[Conclusion]After ACL reconstruction by LARS artificial ligament, indirect connection developed via Sharpey fibers in bone tunnel at the end of LARS artificial ligament and bone interface. The integration of LARS artificial ligament with bone interface has been improved and its intensity is increased.

Objective To summarize the clinical results of treatment of tibia plateau fracture. Methods Forty-six cases of tibial plateau fractures were treated with operation from Oct. 2007 to Oct. 20l2 in General Hospital of PinGdinGshan Coal Industry Groud. AccordinG to Schatzker classification,6 cases were typeⅠ,8 were type Ⅱ,6 were type Ⅲ,4 were type Ⅳ,l2 were type Ⅴ,and l0 were type Ⅵ. Patients in type Ⅰwere treated with open reduction and fixation with screws,other types were treated with open reduction and internal fixation combined with anatomy or lockinG plates and screws. And bone Grafts were used when severe bone defect after reduction. Results Forty-six cases were followed up from 6 months to l8 months( averaGe 8. 5 months ) . AccordinG to the Rasmussen knee score standard,the satisfactory rate was 89. l%( 4l/46 ). Conclusion Different methods of operative treatment of tibia plateau fracture is recommended accordinG to the type of fractures,quality of the bone and the combination of injury. Soft tissue protection should always be kept in mind durinG operation. Delayed operation,Good restitution and fixation,early exercise of joint and late weiGht loadinG are the key factors in the treatment of tibial plateau fracture.

Objective To explore the therapeutic effect of two-stage delayed open reduction and internal fixation on Pilon fractures.Methods The clinical data of 38 patients with type Ⅱ and type Ⅲ Pilon fracture were analyzed retrospectively.According to the Ruedi-Allgower classification,there were 18 cases with type Ⅱand 20 cases with type Ⅲ fracture.All patients underwent temporary fixation with calcaneal traction or external fixation for 7 to 17 days,and then they were treated with delayed surgery when the soft tissue injuries subsided.Results Follow-up was performed for 10 to 18 months.Based on the Mazur classification,the ankle function in 12 cases was excellent,18 cases as good,6 cases as fair and 2 cases as poor,with a satisfactory rate of 78.9％.Conclusion Two-stage delayed open reduction and internal fixation is a reasonable and effective procedure in treating type Ⅱ and type Ⅲ Pilon fractures with advantages of few complications and satisfactory ankle function.

BACKGROUND: When central nervous system is injured, re-expression of nestin protein may enhance the anti-injury ability of cells and be advantageous to the repair of focus of injury.OBJECTIVE: To explore the reaction of nerve stem cell (NSC) in permanent brain ischemia through NSC migration and the change of nestin protein expression.DESIGN: A randomized and controlled verification research with experimental animals as subjects.SETTING: Anatomy teaching and research offices in a training school and a university.MATERIALS: The experiment was done in the Teaching and Research Office of Humane Anatomy in Medical College of Xi'an Jiaotong University from October 1999 to January 2001. Totally 75 healthy SD rats were selected and randomly divided into normal control group, experiment group and sham-operation group. Twenty-five animals were in each group. Heads of animals were cut and brain was got out at the 1st, 3rd, 7th, 14th and 28thdays after operation, 5 animals at each time.METHODS: The model was rats with permanent cerebral ischemia. Immunohistochemical dyeing methods were used to observe NSC migration,change of marker of NSC and nestin protein at the 1st, 3rd, 7th, 14th and 28th day after cerebral ischemia.MAIN OUTCOME MEASURES: ①Results of immunohostochemicaldyeing. ②Migration length of nestin+ cells in anterior subentricular zone (SZa) region of brain tissue at normal status and at different time points after cerebral ischemia. ③Number variation of nestin+ cells at different timepoits after ischemia near the ischemic region.RESULTS: Through nestin immunohistochemical dyeing, it was found that NSC in normal brain tissue mainly existed in subependymal zone (SEZ)region. NSC of SEZ migrated in the direction of ischemic region along ventri- corpus callosum after ischemia. Among them, it reached the farthest at the 7th day after ischemia. More nestin+ cells appeared near ischemic region at the 1st day, and then reduced little by little 3 days later.CONCLUSION: NSC has certain reactive ability to ischemic brain injury.Expression of nestin protein near ischemic region may be a kind of protection to injury.

Stimulator of interferon genes (STING) is a newly discovered adaptor protein in the innate immune system and plays an important role in innate immune response mediated by cytoplasmic DNA. Double-stranded DNA recognition receptors in cells are mediated by STING protein to produce type I interferon and other cytokines. Inadequate innate immune response and anti-hepatitis B virus (HBV) specific immune response are important causes of chronic HBV infection. This article introduces the discovery of STING and the latest research advances in its structure, briefly elaborates on the mechanism of activation of the STING signaling pathway, summarizes the research advances in the interaction between the STING signaling pathway and HBV, and points out the potential value of STING in clinical treatment.

A fluorescence method is found for determination of bovine serum albumin (BSA). The method is based on the interaction of fluorescein with BSA to form a complex in Tris-HCl buffer of pH 2.2. The complex exitation wavelength is 467 nm, the emission wavelength is 515 nm. The linear measurement rang is between 1.8-500 mg/L for BSA, F = 26.776 C + 2.8082, r = 0.9999. This method is sensitive,steady,and low cost for determination of BSA.
Animals ; Cattle ; Drug Interactions ; Fluorescein ; chemistry ; Serum Albumin, Bovine ; analysis ; Spectrometry, Fluorescence ; methods