Objective To investigate the expression of NF ?B in the lungs of adjuvant arthritis (AA) rats and to explore its possible role in the pathogenesis of rheumatoid arthritis (RA) pulmonary involvement.Methods The level of active NF ?B protein was detected by Western blot and the level of NF ?BmRNA by in situ hybridization histochemistry The intensity of expression was analyzed by micro image analysis system.Results The levels of active NF ?B protein and NF ?B mRNA in the lungs of AA rats were much higher than those of the controls ( P

Objective:To study the effect of SH2-B? on asthma.Methods:After inhibition of SH2-B?,the cells in the bronchoalveolar lavage fluid(BALF) were counted,and IL-4 and IgE in BALF,sera and culture supernants of spleen cells were measured by means of ELISA.The changes of the airway resistance and lung function were analyzed by means of animal lungs function analysis system.Results:Compared with that of the asthmatic group,1)Airway resistance to methacholine(MCh) was decreased significantly in anti-SH2-B? group(P

thoracic, but the density of SKLI in the enlargement of the cervical segments is much higher than any other cervical segments. No SKLI was found in the ventral horn. It was also discussed that substance k may act as a neurotransmitter in nervous system.

Objective To observe the dynamic changes of CPP32 mRNA in hippocampus following temporary whole cerebral ischemia in rats and to explore the mechanism of neuron apoptosis induced by cerebral ischemia.Methods The model of temporary whole cerebral ischemia in rats were induced by carotid artery negative pressure shunt and the expression of CPP32 mRNA were detected with fluorescence quantify RT PCR.Results Compared with the naive group, the expression of CPP32 mRNA did not increase insignificantly in sham operation group. Compared with sham operation group, the expression of CPP32 mRNA in ischemia group trended to increase at 6 h after ischemia and increased by 53% at 12 h and 223% at 24 h after ischemia. It kept in high level and decreased at 72 h after ischemia.Conclusion The expression of CPP32 mRNA in hippocampus could be induced by temporary whole cerebral ischemia. It began to increase at 6 h, reached its fastigium at 24 h and tended to decrease at 72 h after ischemia. The enhanced gene transcription of CPP32 plays an important role in the neuron apoptosis induced by cerebral ischemia.

AIM: To explore the regulatory mechanism of n erve growth factor (NGF) on neurokinin A in the experimental asthmatic guinea pi g. METHODS: Radioimmunoassay was used to determine the alteration of neurokinin A levels in the lower respiratory tract and visceral sensory afferent sites while NGF was absent (inhalation of NGF antibody through nasal cavity) in the asthmatic guinea pig. RESULTS: The contents of neurokinin A in the trachea, bronchus, lung, C 7-T 5 spinal ganglia and the correspondent spinal dorsal horn, nodose ganglia and solitary nucleus area in the experimental asthmatic guinea pig with the absent of NGF in the respiratory tract were much lower than those in the as thmatic and control groups (P

Objective To study the effects of calcitonin gene related peptide(CGRP) and nerve growth factor(NGF) on the expression of(c-junmRNA) and protein in striate cortex of rats with transient global cerebral ischmia/reperfusion. Methods In situ hybridization,immunohistochemistry and microscope image analysis were used. Results The expression of c-jun mRNA in ischemia/reperfusion(I/R) group was increased as compared with sham group(P

thoracic. No immunoreactive product was seen in the ventral horn. The possible functions of CGRP in substantia gelatinosa of the spinal dorsal horn were discussed.

Immunohistochemical study revealed the distributions of substance P, neurokinin A, neurokinin B and calcitonin gene related peptide immunoreactive nerve fibers in the medulla, cortex and capsule of tracheobronchial lymph nodes in 10 guinea pigs. More immunopositive fibers were found to be distributed in the area around the blood vessels and lymph sinuses, less in the area distant to blood vessels. The four kinds of positive immunoreactive nerve fibers were also seen within the wall of the blood vessels. The results provide some morphological basis for the study of neuroimmunomodulation.

Objective: Our purpose was to explore the protective role of Salvia Miltiorrhiza (SM) on gentamicin (GM) ototoxicity. Methods: We used NADPH-diaphorase in histochemical staining and image quantitative analysis technique, combined with auditory brainstem response (ABR) measurement to investigate the change of nitric oxide synthase (NOS) activity in cochlea of guinea pig after the injection of GM and SM. Results: Gebtamicin and SM significantly reduced NOS activity in cochlear and the threshold of ABR compared with GM alone (P<0.01); and the threshold of ABR was in high correlation with NOS activity (rGM= －0.8236 ～ －0.8662; rSM+GM= －0.8628 ～ －0.9172, P<0.01). Conclusion: Salvia Miltiorrhiza can reduce NOS activity in cochlea, alleviate GM ototoxicity, and ameliorate hearing function.

AIM: To explore the regulatory mechanism of nerve growth factor (NGF) on substance P in the experimental asthmatic guinea pig. METHODS: Radioimmunoassay was used to determine the alteration of substance P levels in the lower respiratory tract and visceral sensory afferent sites while NGF was absent (inhalation of NGF antibody through nasal cavity) in the asthmatic guinea pig. RESULTS: The contents of substance P in the trachea, bronchus, lung, C7 - T5 spinal ganglia and the correspondent spinal dorsal horn, nodose ganglia and solitary nucleus area in the experimental asthmatic guinea pig with the absent of NGF in the respiratory tract, were much lower than those in the asthmatic and control groups (P