Objective To evaluate the long-term curative effect of bronchial artery embolization on massive hemoptysis in patients with pulmonary tuberculosis. Methods Bronchial arterial embolization with PVA grain and silk segment was performed in 42 cases of patients with hemoptysis of secondary pulmonary tuberculosis. Of them, 27 cases were bilateral pulmonary tuberculosis and 15 lateralized. The bloody bronchial arteries were confirmed by bronchial arteriography, and the PVA grains or silk segments were injected slowly into the target vessels until hemorrhage stopped. Results After one time embolization, hemoptysis stopped immediately in 34 cases, 7 cases still had little hemoptysis,and one case of hemorrhage was unable to be controlled. The short-term efficacious rate was 97.6%,and no severe complications occurred. Followed up 6 months to 7 years after treatment,24 cases were clinical cure,7 cases obviously effective,5 cases effective,and 6 cases recurrent.The long-term effective rate is 85.7%. Conclusion Embolization of bronchial artery with PVA grain and silk segment was a safe and effective method for controlling hemoptysis of pulmonary tuberculosis, and its combination with anti-tuberculosis treatment could consolidate the curative effect.

Objective To discuss the safety and clinical effect of fine needle single-step centesis in percutaneous endoscopic nephrolithotomy for renal staghorn calculi. Methods Percutaneous endoscopic nephrolithotomy with fine needle single-step centesis was employed in 75 patients (single-step group) with renal staghorn calculi, and percutaneous endoscopic nephrolithotomy with two-step centesis was adopted in other 75 patients with renal staghorn calculi (two-step group). The clinical effect and the incidence of complications were compared between the two groups. Results The placement of drainage catheter was successfully accomplished in all 150 patients. In single-step group the operation time was 18-45 minutes with a mean of 36 minutes; the mean blood loss during the procedure was about 5 ml. After the treatment, massive bleeding occurred in 3 cases that needed blood transfusion, and residual stone was observed in 6 cases. In two-step group the operation time was 16-42 minutes with a mean of 34 minutes; the mean blood loss during the procedure was about 7 ml. After the treatment, massive bleeding occurred in 7 cases that needed blood transfusion; one of them had renal pseudoaneurysm and the bleeding was stopped after renal artery embolization treatment; and residual stone was observed in 7 cases. No procedure-related perirenal organ injury was seen in single-step group, while in two-step group pneumothorax (n=1) and injury of splenic flexure of colon (n=1)were found. Conclusion In performing percutaneous endoscopic nephrolithotomy, fine needle single-step centesis is more safe and effective than conventional two-step centesis.

The different levels and clinical significance of high sensitivity C reactive protein(hs-CRP) , tumor necrosis factor-a ( TNF-α) , erythrocyte sedimentation rate ( ESR) , and urinary monocyte chemoattractant protein-1 (uMCP-1) in 84 type 2 diabetic patients with different urine albumin excretion (UAE) were observed. The results indicated that the levels of hs-CRP,TNF-α,urinary MCP-1 ,and ESR in diabetic patients were significantly higher than those in control group,and the first three indexes increased with the levels of UAE. The results suggest that diabetic nephropathy seems to be correlated with inflammatory reactions.

Objective To explore the technique and clinical effect of Fogarty catheter in treatment of acute limb arterial embol-ism.Methods Eight cases of acute limb arterial embolism treated by Fogarty catheter were analyzed retrospectively.The technique of thrombectomy,curative effect,complications,prognosis,and 6-24 months follow-up results were assessed to evaluate the safe-ty,effectiveness and operative skills.Results Seven cases were cured,and 1 case was efficacious.The endangium injury and vaso-spasm was occurred in 1 case,respectively.During follow-up from 6 to 24 months,6 cases recovered activity,1 case was hemiplegia because of cerebral infarction after 12 months,and 1 case died of respiratory function failure after 6 months.Conclusion Fogarty catheter is safe and effective in treatment of acute limb arterial embolism.

Objective To evaluate the interventional management in emergency splanchnic bleeding and the application value.Methods 27 patients with emergency splanchnic bleeding underwent the interventional management in our hospital from May of 2003 to January of 2006 were reviewed.The sites and causes of the splanchnic hemorrhage were verified through selective DSA,using different methods and materials for percutaneous transarterial embolization.Results No bleedings recurred in all 27 patients within 18 months after the treatment.3 patients of advanced hepatic carcinoma died in 6 months because of non-splanchnic bleeding etiology.Conclusions Selective angiography is a veracious way to detect the location and cause of emergency splanchnic hemorrhage.The choice of different selective arterial embolizations and embolic materials can effectively and promptly cease the bleeding with rescuing the patients.

Objective To suty DSA features of the splencin artery which provides blood supply to the tumor in the patients with hepatic carcinoma,to improve the clinical results of interventional therapy.Methods DSA was performed in 3 patients with hepatic carcinoma and the blood supply of tumors by splenic artery was found.The DSA features were analysed and the interventional embolization was carried out at the same time.Results After super-selective embolization of the splenic artery,obvious reduction of the tumor-feeding microvessels and marked shrinking of the lesion were observed in all cases.Conclusion Splenic artery as one of the supply arteria to the tumors in hepatic carcinoma is very important to familiarize that in transcatheter arterial chemoembolization for hepatic carcinoma.

Objective To assess the clinical value of interventional treatment for bone and soft-tissue tumors. Methods Selective angiography, transcatheter intra-arterial chemotherapy and/or embolization were performed in 28 patients with pathologically-proved bone and soft-tissue tumors. After treatment the clinical response and pathological changes were observed, and the results were analyzed. Results After transcatheter intra-arterial chemotherapy and/or embolization, relieving or even disappearing of the pain was seen in 23 patients, subside of soft-tissue swelling together with regression of the tumor was seen in 19 patients. Twenty-two patients underwent surgical resection of the lesion one week afte.r interventional treatment. Pathologically, cellular degeneration, necrosis and various degrees of liquefaction were demonstrated on the tumor specimen, which were more obvious in patients treated with embolization. Limp-sparing resection was adopted in 66.7% of patients (10/15). Conclusion lnterventional therapy is an effective method for bone and soft-tissue tumors and it is worth popularizing this technique in clinical practice.

Objective To analyze the clinical characteristics , diagnosis, therapy and prognosis of new diagnosed pri-mary gastric diffuse large B cell lymphoma ( PGDLBCL) and to discuss the efficacy of rituximab .Methods Between Jan 2005 and May 2012 , twenty-six new-diagnosed PGDLBCL patients were reviewed retrospectively .The clinical characteris-tics, diagnosis, therapy, results and prognostic factors were analyzed .Results There were 14 males and 12 females.Their age ranged from 25 to 82 (median, 50.1) years old.The most common symptom was stomachache .Treatment strategies were chemotherapy alone ( n=9) [ scheduled as cyclophosphamide , doxorubicin , vincristine and prednisone ( CHOP) and CHOP-like] and chemotherapy combined with rituximab (n=17), followed by radiotherapy of the stomach with or without regional nodes .All clinical and pathological features were similar between the two groups .The median follow-up time was 40 months.The overall response rate was 100%(9/9)in CHOP group, including 55.56%(5/9) CR, and 93.75%(15/16) in RCHOP group including 50%(8/16) CR (P>0.05).The total PFS and OS of 5 years were 60.3%and 74.4%respectively.The PFS in CHOP group and RCHOP group was 66.7% and 58.9%, respectively,and the OS was 66.7%and 84.6%, respectively.Although the OS of RCHOP group was much better than that of CHOP group , there was no sta-tistically significant difference.Univariate analysis showed that IPI (P<0.05) and Lugano staging (P<0.05) were inde-pendent factors of survival in patients with PGDLBCL .Conclusion Chemotherapy could be the first-line therapy of PGDL-BCL.The overall survival rate might be increased by adding rituximab to chemotherapy .The Lugano stage and IPI are im-portant prognostic factors .

Objective To investigate the therapeutic effect and adverse effects of the teniposide-based regimen in patients with primary central nervous system lymphoma (PCNSL). Methods Between March 2011 and July 2013, 16 patients with PCNSL were diagnosed and treated. The clinical characteristics, diagnosis,therapy, results and adverse effects were analyzed. Results Totally 16 patients were enrolled and diagnosed as primary central nervous system diffuse large B-cell lymphoma. All patients received teniposide-based regimen chemotherapy and 9 patients received teniposide plus rituximab. The overall response rate was 87.5 % (14/16), including 10 cases of CR and 4 cases of PR. With a median follow-up of 13.5 months, the progression-free survival (PFS) and overall survival (OS) rates of 2 years were 29.9 % and 66.7 %, respectively. The mainly hematological adverse events were neutropenia, including grade 3 in 4 cases (25 %) and grade 4 just in one case. There was one case of treatment related death. Conclusions The response rate of teniposide-based regimen for PCNSL is promising. The 2 year PFS and OS rates are even higher than results of traditional high-dose methotrexate regimen. The teniposide-based regimen is well tolerated, and the adverse events are acceptable.

Objective: To investigate the expression and mutation status of tensin family member 4 (TNS4) gene in various prostate cancer cell lines, and to explore its potential molecular mechanism. Methods: The expression of TNS4 gene in several prostate cancer cell lines was detected by RT-PCR. The full-length cDNA of TNS4 gene was amplified for the restriction endonuclease hydrolysis and DNA sequencing analysis. Meantime, the recombinant plasmids carrying wild- or mutant-type TNS4 gene were constructed and transfected into prostate cancer PC-3 cells, then the effects of TNS4 over-expression on the mRNA and protein expressions as well as the localization of signal transducer and activator of transcription 1 (STAT1) were detected by RT-PCR, Western blotting and immunofluorescence microscopy, respectively. Results: As compared with benign prostatic hyperplasia BPH1 cells, TNS4 gene was lowly expressed in prostate cancer cell lines DU145, LNCaP and 22Rv1 (all P < 0.01), and nearly undetectable in prostate cancer PC-3 cells. The analysis of full-length TNS4 cDNA sequencing revealed that two sites of single point mutation were identified at T427C and G1455A of TNS4 gene derived from DU145 and LNCaP cells, and the former caused the corresponding amino acid transition (S143P). The recombinant plasmids pcDNA3.1 (+) -TNS4-Mut (TNS4-Mut) and pcDNA3.1 (+) -TNS4-WT (TNS4-WT) were successfully established. After transfection with TNS4-Mut or TNS4-WT, the expression levels of TNS4 mRNA and protein and STAT1 protein in PC-3 cells were significantly increased (all P < 0.05), but the expression level of STAT1 mRNA was not changed (P > 0.05). Additionally, the over-expression of mutant TNS4 significantly promoted nuclear translocation of STAT1 protein (P < 0.01). Conclusion: TNS4 gene is lowly expressed in prostate cancer cell lines, and there is a novel TNS4 mutant (S143P). TNS4 over-expression can up-regulate the expression of STAT1 protein, furthermore the mutant TNS4 can alter the location of STAT1 protein.