Objective: To develop a method of retinal microglial cell culture to study the function of the microglial cell in diabetic retinopathy. Methods: Microglia were activated with LPS. Immunocytochemistry, con-focal microscopy, flow cytometry, MTT and ELISA were applied to observe the morphological characters, quantity, and functional changes of the microglia. Results: The purity of the microglia was up to 96% as determined by immunostaining and flow cytometry. Some morphological changes of microglia were observed after treatment with LPS, but their quantity kept stable. Cytokine TNF-? released from microglia increased significantly. Conclusion: The isolated microglial cells are pure by using this culture system, which would provide a valuable tool for studying mechanisms of microglial alterations in diabetic retinopathy.

OBJECTIVE: To investigate the usefulness of anterolateral thigh flap for reconstruction of the defect after maxillectomy. METHOD: Seventy-two cases with defect after maxillectomy were constructed with anterolateral thigh flap in our department during 10 years. All cases were undertaken total maxillectomy. RESULT: All flaps except one were transferred successfully. Patients were satisfied with their appearances. No serious complications had happened. CONCLUSION Anterolateral thigh flap could be an donor flap for the reconstruction of the defect after maxillectomy.
Face ; surgery ; Free Tissue Flaps ; Humans ; Maxilla ; surgery ; Reconstructive Surgical Procedures ; Thigh

Objective To evaluate effectiveness of application with FOCUS harmonic scalpel in papillary thyroid carcinoma (PTC) surgery.Methods 110 patients with PTC between June 2013 and December 2013 were divided into two groups randomly as harmonic scalpel group (group H) and conventional group (group C).Incision length,operative time,estimated blood loss,drainage volume within 24 h,in the second 24 h and in total after operation,the number of lymph node retrieval,post-operative complication and hospital stay were compared between two groups.Rusults There is significant different in factors of incision length [(5.5±0.4) cm in group H and (6.6±0.5) cm in group C (P ＜ 0.05)],operative time [(54.4±8.7) min in group H and (73.3±13.9) min in group C (P ＜ 0.05)],estimated blood loss [(14.2±6.0) ml in group H and (29.8±5.0) ml in group C (P ＜ 0.05)].There were no differences in drainage volume,the number of lymph node retrieval,post-operative complication and hospital stay between two group (P ＞ 0.05).Conclusion FOCUS harmonic scalpel could reduce incision length,operative time,estimated blood loss in PTC surgery.