1.Effect of BRL-37344 on ion channel in guinea pig cardiac myocytes
Yun XIU ; Xiangru KONG ; Lanxiang WU
Journal of Third Military Medical University 2003;0(11):-
Objective To study the biological effect of ?3 adrenoceptor-activated electrophysiological activities in cardiac myocytes. Methods Changes of transient outward potassium ( Ito) and L-type calcium current ( ICa-L) in primary cultured guinea pig cardiacmyocytes were detected with the standard whole-cell patch clamp technique about 5 -10 min before and after administration of ?3 adrenoceptor agonist,4--2-hydroxy-( 3-chlorophenyl) ethyl-aminopropylphenoxyacetate ( BRL-37344; BRL). Results BRL could significantly increased the Ito in guinea pig cardiac myocytes with the I-V curve up-moved. When the membrane potential reached to + 80 mV,the density current increased from 6. 11 ? 1. 03 pA/pF to 8. 46 ? 2. 07 pA/pF ( n = 6,P =0. 013 064). BRL ( 10 -6mol/L) could significantly increase the ICa-Lin guinea pig cardiac myocytes,which was ( 2. 30 ?0. 75) -fold higher than in control group( n =5,P =0. 0063). When the membrane potential increased to + 10 mV ,the current,increased from 89. 25 ? 17. 83 pA to 205. 00 ? 72. 24 pA ( n = 5,P = 0. 006 3). Conclusion BRL-37344 can increase the transient Itoand ICa-L,thus further regulating the cardiac activities.
3.On connotation of Jin's three-needle technique.
Qing YUAN ; Long-Lin LIU ; Xiu-Jin SHEN ; Fei CHEN ; Yun-Cai WU
Chinese Acupuncture & Moxibustion 2014;34(7):701-704
Jin's three-needle technique is named after Professor JIN Rui. The connotation of Jin's three-needle technique is summarized by his followers through long-term teaching and clinical practice. His principle and method of point combination are based on syndrome differentiation of acupuncture. And his unique needling technique and treating principle of mental tranquilization also play an important role in his clinical practice.
Acupuncture Therapy
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instrumentation
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methods
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Humans
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Needles
4.Duodenal gangliocytic paraganglioma: report of a case.
Chang ZHANG ; Jian WU ; Su-an SUN ; Hai-yan LIU ; Wu-bi ZHOU ; Xiu-fang LI ; Yun JIN
Chinese Journal of Pathology 2012;41(1):55-56
Chromogranin A
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metabolism
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Diagnosis, Differential
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Duodenal Neoplasms
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metabolism
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pathology
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surgery
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Ganglioneuroma
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metabolism
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pathology
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Gastrointestinal Stromal Tumors
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metabolism
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pathology
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Humans
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Male
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Middle Aged
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Neurofibroma
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metabolism
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pathology
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Paraganglioma
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metabolism
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pathology
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surgery
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Phosphopyruvate Hydratase
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metabolism
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S100 Proteins
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metabolism
5.Impurity removal technology of Tongan injection in liquid preparation process.
Xu-fang YANG ; Xiu-hai WANG ; Wei-rong BAI ; Xiao-dong KANG ; Jun-chao LIU ; Yun WU ; Wei XIAO
China Journal of Chinese Materia Medica 2015;40(16):3200-3203
In order to effectively remove the invalid impurities in Tongan injection, optimize the optimal parameters of the impurity removal technology of liquid mixing process, in this paper, taking Tongan injection as the research object, with the contents of celandine alkali, and sinomenine, solids reduction efficiency, and related substances inspection as the evaluation indexes, the removal of impurities and related substances by the combined process of refrigeration, coction and activated carbon adsorption were investigated, the feasibility of the impurity removal method was definited and the process parameters were optimized. The optimized process parameters were as follows: refrigerated for 36 h, boiled for 15 min, activated carbon dosage of 0.3%, temperature 100 degrees C, adsorption time 10 min. It can effectively remove the tannin, and other impurities, thus ensure the quality and safety of products.
Adsorption
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Charcoal
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chemistry
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Drug Compounding
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instrumentation
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methods
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Drug Contamination
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prevention & control
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Drugs, Chinese Herbal
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chemistry
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isolation & purification
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Quality Control
7.Ionic mechanism underlying chance of L-type Ca~(2+) current in isolated rat ventricular myocytes induced by hypercholesterolemia
Hong WU ; Xiu-Dong JIN ; Yun-Long BAI ; Li-Xin GUAN ; Yan-Jie LV ; Bao-Feng YANG ;
Chinese Pharmacological Bulletin 1987;0(03):-
Aim To investigate the effect of hypercholesterolemia on L-type Ca2+(ICa-L) current and intracellular calcium concentration ([Ca2+]i) in single ventricular myocytes of hypercholesterolemic rats.Methods 12 male wistar rats were randomly divided into two dietary groups:a group fed a normal diet(n=6)and a group fed high-cholesterol diet(n=6) for 4 weeks,respectively. The level of serum lipid was examined.Zymolytic method was used to isolate single ventricular myocytes from hypercholesterolemic and normal rats,which were loaded with Ca2+-sensitive fluorescent indicator Fluo-3/AM.[Ca2+]i represented by fluorescent intensity(FI)was measured by laser scanning confocal microscope.Whole cell patch clamp technique was used to record ICa-L.Results There was no significant influence exhibited on TG level.However, the serum total cholesterol(TC)level of hypercholesterolemic rats was much higher than that of model control group; at the test potential of 0 mV, ICa-L decreased from(-8.56?1.29)pA/pF(Control)to(-5.24?0.90) pA/pF(HC)(n=6 in each group,P
8.Atorvastatin Inhibits The Development of Macrophages to Foam Cells
Peng WU ; Ying-Feng LIU ; Yun-Qin CHEN ; Donghui LIANG ; Xiu-Li ZHANG ; Yongyuan CHEN ; Wen-Zhong CHEN ;
Chinese Journal of Hypertension 2007;0(04):-
Objective To investigate effects of atorvastatin on the development from macrophages (HMDM) to foam cells.Methods Monocytes were isolated from human peripheral blood by Ficoll-Hypaque density gradient centrifugation and plastic adsorptive process.The isolated cells were stimulated by phorbol 12-myristate 13-acetate (PMA) (50 nmol/L) for 48 h and transformed to macrophages.Macrophages were co-incubated with 80 mg/L ox- idized low density lipoprotein (ox-LDL) and atorvastatin (0-100 ?mol/L),respectively for 0,6,12 and 24 h. Total cholesterol (TC),free cholesterol (FC) and protein (Pro) in cultured cells were quantitatively analyzed by high performance chromatography (HPLC) analysis and modified Lowry protein assay.Results When macropha- ges were incubated with 80 mg/L ox-LDL,the ratio of TC/Pro was greater than 20,and large amount of lipid drop- lets were displayed indicating the formation of foam cells.Atorvastatin decreased TC/Pro ratio in foam cells in a concentration and time dependent manner (0-100 ?mol/L)(P
9.Metabonomic Characterization of The Low-grade Human Astrocytomas and Meningiomas Using Magic-angle Spinning 1H Nuclear Magnetic Resonance Spectroscopy and Principal Component Analysis
Wenxue CHEN ; Haiyan LOU ; Hongping ZHANG ; Xiu NIE ; Yun XIANG ; Yongxia YANG ; Guangyao WU ; Jianpin QI ; Yong YUE ; Hao LEI ; Huiru TANG ; Feng DENG
Progress in Biochemistry and Biophysics 2008;35(10):1142-1153
Metabolic characteristics of 39 human brain tumor tissues, including 15 astrocytomas, 13 fibroblastic meningiomas and 11 transitional meningiomas from 39 individual patients, have been studied using high resolution magic-angle spinning (HRMAS) 1H NMR spectroscopy in conjunction with principal component analysis (PCA). With rich metabolite information, 1H NMR spectra showed that the tumor-tissuc metabonome was dominated by lipids, lactate, myo-inositol, ereatine, choline metabolites such as choline, phosphocholine and glycerophosphocholine, amino acids such as alanine, glutamate, glutamine, taurine, N-acetyl-aspartate and glutathione. PCA of the tumor NMR spectra clearly showed metabonomic differences between low-grade astrocytomas and meningiomas whereas such differences were more moderate between fibroblastic and transitional meningiomas. Compared with meningiomas, the low-grade astrocytomas had higher levels of glycerophosphocholine, phosphocholine, myo-inositol and creatine but lower levels of alanine, glutamate, glutamine, glutathione and taurine. The N-acetyl-aspartate level was low but detectable in low-grade astrocytomas whereas it was not detectable in meningiomas. It is concluded that tissue metabonomics technology consisting of HRMAS 1H NMR spectroscopy and multivariate data analysis (MVDA) offers a useful tool (1) for distinguishing different types of brain tumors, (2) for providing the metabolic information for human brain tumors, which are potentially useful for understanding biochemistry of tumor progression.
10.The distribution and significance of insulin-like growth factor binding protein in patients with liver fibrosis.
Xiao-xia TIAN ; Gui-xiu QIN ; Ke-ming YUN ; Li-xin LIU ; De-wu HAN
Chinese Journal of Hepatology 2006;14(11):858-860
Adolescent
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Adult
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Aged
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Female
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Humans
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Insulin-Like Growth Factor Binding Proteins
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metabolism
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Liver
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metabolism
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Liver Cirrhosis
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metabolism
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pathology
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Male
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Middle Aged
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Young Adult