3.Expression of stromal cell-derived factor-1 in endometriosis by steroid receptor coactivatorson steroid hormone
Xiu SHI ; Wei XU ; Ying SUN ; Huihua DAI ; Xiuli WANG
Chinese Journal of Obstetrics and Gynecology 2014;49(2):114-119
Objectives To study the expression patterns of steroid receptor coactivators (SRC) and steroid-induced stromal cell-derived factor-1 (SDF-1) in endometriosis,and to explore the roles of SRC in the steroid-induced SDF-1 expression endometriosis.Methods From May 2010 to October 2012,16 endometriosis cases at stages Ⅲ or Ⅳ according to the revised American Society for Reproductive Medicine classification undergoing surgery in the First Affiliated Hospital to Nanjing Medical University were enrolled in this study.Their ectopic endometrium were from ovarian endometriomata which were identified pathologically with 9 cases at proliferative phase and 7 cases at secretory phase.The normal endometrium were acquired from the healthy women with normal menstrual cycle (n =10,proliferative phase =5,secretory phase =5).The mnRNA levels of SRC and SDF-1α during the menstrual cycle were detected by quantitative real-time polymerase chain reaction.Ectopic endometrium stromal cells were purified and cultured in medium containing 17β-estradiol (10-8mol/L) or 17β-estradiol (10-8 mol/L) + progesterone (10-6 mol/L).At 24,48,72 and 96 hours,the supernatants were collected to measure SDF-1α expression by ELISA.Ectopic endometrium stromal cells were transfected respectively with siRNA of SRC-1 and SRC-2 using lipofectamine.Two days after transfection,17β-estradiol (10-8 moL/L) or 17β-estradiol (10-8 mol/L) + progesterone (10-6 mol/L) were added into the media.On the third day after the steroid hormones treatment,the media were collected to quantify SDF-1α expression with ELISA.Results (1) Cyclical changes: the SRC-1,SRC-2 and SDF-1 α showed marked cyclic differences in normal endometrium (P < 0.05).In proliferative phase and secretory phase,the SRC-1,SRC-2 and SDF-1 α were 5.6 ± 1.2,3.8 ± 1.1,2.7 ± 0.5 and 2.6 ± 1.0,2.1 ± 1.0,1.6-± 0.5,respectively.There was no periodic variation in the expression of SRC-1,SRC-2 and SDF-1α in ectopic endometrium throughout the menstrual cycle.(2) Steroid-induced SDF-1α expression in ectopic endometrium stromal cells: the 17β-estradiol-induced SDF-1α expression was (1 803 ± 196),(2 272 ± 261) and (2 162 ± 258) ng/L at 48,72 and 96 hours.At the same time points,the SDF-1α expression induced by 17β-estradiol and progesterone was (1 307 ± 150),(1 518 ± 301) and (1 550 ± 144) ng/L,respectively.There was significant difference between two groups (P <0.05).(3) The effects of SRC silencing on steroid hormones-induced SDF-1 α expression in ectopic endometrium stromal cells: the expression of 17β-estradiol-induced SDF-1α at 72 hours was significantly decreased from (2 313 ± 357) ng/L to (1 155 ± 244) ng/L after the silencing of SRC-1 (P < 0.05).After the silencing of SRC-2,the 17β-estradiol-induced SDF-1 α at 72 hours was (1 958 ±324) ng/L.There was no significant difference compared with the before the silencing (P > 0.05).The expression of SDF-1 α at 72 hours induced by 17β-estradiol + progesterone was (1 534 ± 449) ng/L and (2 051 ± 380) ng/L respectively before and after the silencing of SRC-2 and showed the significant difference (P < 0.05).Conclusion During the expression of SDF-1 α regulated by steroids in ectopic endometrium cells,SRC-1 is the major coactivator of 17β-estradiol and SRC-2 is the major coactivator of progesterone.
4.Correlation between Angiotensin Converting Enzyme Gene Polymorphism and Kawasaki Disease
dong-hai, LIU ; xiu-ying, WANG ; yi, XU
Journal of Applied Clinical Pediatrics 1986;0(01):-
Objective To investigate the correlation between angiotensin converting enzyme(ACE) gene polymorphism and kawasaki disease(KD).Methods A 287 bp Alu fragment in intron 16 of the ACE gene was used as insertion(I)/deletion(D) polymorphism marker. The ACE genotype of 28 children (10 children complicated coronary dilataltion) with KD and 35 healthy controls were detected by polymerase chain reaction (PCR), and ACE concentration in blood serum was measured by ultraviolet-spectrophotometer assay.Results 1.The ACE concentration was significantly higher in KD group than that in healthy control group(P
5.Hypertrophic cardiomyophthy: a family report.
Hai-Yun DONG ; Xiu-Ying WANG ; Yi XU
Chinese Journal of Contemporary Pediatrics 2010;12(6):1 p folowing 512-1 p folowing 512
Adolescent
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Adult
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Cardiomyopathy, Hypertrophic
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genetics
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Child
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Humans
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Male
6.Clinicopathologic analysis of ovarian gonadoblastoma.
Xiu-Li WU ; Lian XU ; Ying HE ; Na YU ; Wei-Wei WU ; Kai-Xuan YANG
Chinese Journal of Pathology 2009;38(6):418-419
Adolescent
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Alkaline Phosphatase
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metabolism
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Antineoplastic Combined Chemotherapy Protocols
;
therapeutic use
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Bleomycin
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therapeutic use
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Cisplatin
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therapeutic use
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Diagnosis, Differential
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Dysgerminoma
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pathology
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Etoposide
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therapeutic use
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Female
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Gonadoblastoma
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drug therapy
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metabolism
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pathology
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surgery
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Humans
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Hysterectomy
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methods
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Inhibins
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metabolism
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Isoenzymes
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metabolism
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Ovarian Neoplasms
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drug therapy
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metabolism
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pathology
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surgery
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Young Adult
7.Efficacy of treating the early-stage chronic angle closure glaucoma with laser peripheral iridoplasty
Xiu-Duo, LIU ; Chun, SHI ; Jian, YING ; Hui-Di, XU ; Wei, ZHANG
International Eye Science 2015;(4):656-658
AIM: To evaluate the safety of the laser peripheral iridoplasty ( LPIP ) for the early-stage chronic primary angle-closure glaucoma ( ECPACG) .
METHODS:Sixty-five eyes of 36 patients with ECPACG received LPIP. At preoperative and postoperative, patients were examined with intraocular pressure ( IOP ) , anterior chamber, optical coherence tomography ( OCT) , visual acuity, visual field, fundus and complications. The mean follow-up was 18. 2±6. 7mo (ranged 12~24mo).
RESULTS: The preoperative average IOP were 23. 8±5.6mmHg, postoperative 1wk, 1, 3, 6, 12mo and in the last follow-up time the average IOP were 18. 7±3. 8, 17. 9±3. 2, 17. 6±3. 5, 18. 4±3. 7, 18. 6±3. 7, and 18. 6±7. 8mmHg. There was statistical difference comparing with preoperative (P<0. 05, decreasing average 6. 5±3. 1mmHg compared with the preoperative, the difference was statistically significant (t=5. 32, P<0. 05). Postoperative 1wk, 1, 3, 6mo, the trabecular-iris angle ( TIA500 ) and the angle opening distance at 500μm ( AOD500 ) had the statistical difference comparing with preoperative ( P <0.05). At Postoperative 1a and the last follow-up time, the TIA500 and AOD500 did not have statistical difference comparing with preoperative (P>0. 05). The postoperative visual acuity, visual field, fundus had not changed. There were no complications found.
CONCLUSION:LPIP is safe, and has the short time effect in the treatment of ECPACG. With elapse of time, the effect of LPIP is weakened. We can repeat the treatment.
8.Expression of Lck in renal tubule epithelial cells of lupus nephritis
Ying-Hui DENG ; Qing-Gang LI ; Chong-Yu REN ; Xiu-Hong XU ;
Chinese Journal of Rheumatology 2003;0(07):-
Objective To investigate the Lymphocyte-specific protein-tyrosine kinase(Lck)gene ex- pression in the renal tubule epithelial cells(TECs)of lupus nephritis,and the effect of interlenkin-2(IL-2) stimulation on its expression.Methods Proximal TECs derived from 6 weeks old spontaneous systemic lupus erythematosus(SLE)BXSB mice were exposed to IL-2(100 U/ml),the expression of Lck mRNA and protein was examined by reverse transcription-polymerase chain reaction(RT-PCR)and immunoblotting respectively. The difference of Lck gene expression before and after IL-2 stimulation was investigated.The expression of Lck protein in TECs of renal tissues of BXSB mice and human with lupus nephritis was observed through im- munohistochemistry.Results The expression of Lck mRNA and protein was very low in cultured TECs of 6 weeks old BXSB mice,but increased sharply after IL-2 stimulation(P
9.The efficacy of 125I radio active particle implantation in the treatment of maxillofacial malignancy of 43 cases
Xiu YAO ; Ling GU ; Ying LIU ; Shuangshuang LI ; Dong WANG ; Lu YANG ; Lihong XU ; Chongjian FU
Journal of Practical Stomatology 2016;32(2):220-224
Objective:To investigate the effects of 125I radioactive particle implantation in the treatment of maxillofacial malignancy. Methods:43 patients with maxillofacial malignancy were treated with 125I radioactive particle implantation.The procedure was carried out according to the treatment planning system(TPS),with the particles spaced uniformly at 1 cm intervals and with the activity of 0.7 m Ciby 41 particles per case on a verage.All patients were followed up for 6-60 months.Results:The whole treatment procedurewas successful,and no particle displaced.The follow-up rate was 93.02% and treatment effective rate(CR+PR) was 90.70%.Norecur-rence was foundinall target areas.The mortality due to tumor was 9.30%and total survival rate was 74.43%.The cumulative survival rate of the patients in 0.5,1,2,3 and 5 years was 93.0%,85.7%,79.3,69.8% and 56.9% respectively.Survival periodon aver-age was 36.06-50.04 months,with the median of 43.05 months.The longest tumor-free survival period was 60 months.Radiation in-jury rate was 20.93% and only level 1 radiation injury was observedinall the cases.Facial nerve dys function was found in 2 cases and recovered after treatment.Conclusion:Treatment of maxillofacial malignancy by implantation of 125I particles is convenient and mini-mally invasive.The treatment canincrease survival rate of the patients and guaran tee the oropharynxes' function.
10.Expression of p57Kip2 and its significance in the classification and differential diagnosis of hydatidiform moles and hydropic abortions.
Ying HE ; Kai-xuan YANG ; Hong-jing WANG ; Lian XU ; Qin HUANG ; Xiu-li WU
Chinese Journal of Pathology 2007;36(3):197-199
Abortion, Spontaneous
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diagnosis
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genetics
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metabolism
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Cyclin-Dependent Kinase Inhibitor p57
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metabolism
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Diagnosis, Differential
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Female
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Gene Expression Regulation, Neoplastic
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Humans
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Hydatidiform Mole
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diagnosis
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genetics
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metabolism
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Immunohistochemistry
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Pregnancy
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Uterine Neoplasms
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diagnosis
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genetics
;
metabolism