1.Application of blood purification of critical disease with non-renal indications in PICU
Xuan XU ; Xiu YIN ; Xiannan CHEN ; Zhichun FENG
Chinese Pediatric Emergency Medicine 2010;17(5):400-403
Objective To investigate the application of blood purification for critical disease with non-renal indications in PICU. Methods We retrospectively analyzed the clinical data of 10 critical disease cases with non-renal indications in PICU admitted from Jan to Dec 2009. Five cases were with acute liver failure,2 with autoimmune disease (1 with Guillaln-Barre syndrome, 1 with systemic juvenile rheumatoid arthritis with macrophage activation syndrome) ,2 with severe sepsis,one with metabolic diseases. Results Four cases were treated with plasma exchange combined with continuous veno-venous hemmofiltration. Three cases were treated with continuous veno-venous hemmofiltration. Three cases were treated with plasma exchange.Conclusion CBP is an effective and safe method in the treatment of critical diseases with non-renal indications in PICU.
2.Application of continuous blood purification in the children with critical diseases
Xuan XU ; Xiu YIN ; Xiannan CHEN ; Zhichun FENG
Chinese Pediatric Emergency Medicine 2010;17(4):318-320
Objective To investigate the application of continuous blood purification in the children with critical diseases. Methods Eighteen critical patients aged 1 ~ 15 years in PICU underwent continuous blood purification(CBP). Fourteen with acute renal failure (ARF) were treated with continuous veno-venous hemmofiltration(CVVH) ,2 with Guillain-Barre syndrome and 2 with Raye's syndrome were treated with plasma exchange(PE). The changes of clinical symptoms, blood biochemistry , blood gas, and oxygenation were analyzed before and after CBP. Results After CVVH treatment, the BUN and creatinine of 14 patients with ARF were decreased from (48.6 ± 14. 8) mmol/L to(28. 9 ±5.4) mmol/L and (634. 3 ±258. 2) μ mol/L to (318.4 ± 143.5) μmol/L,K+ and pH of serum were maintained in the normal range,oxygenation was significantly improved. Breathing difficulties and muscle strength in 2 patients with GBS were ameliorated and successfully weaned from ventilator after PE. Serum ALT,AST and ammonia of 2 cases with Raye's syndrome decreased significantly and they discharged after comprehensive treatment including PE. Bleeding in puncture region were found in 3 patients, hypothermia in 2 patients. During the treatment, vital signs of patients were stable,blood pressure and pulse were not fluctuated. Conclusion CBP is an effective and safe method in the treatment of critical diseases in children.
3.Effects of intermittent catheterization methods on the urinary tract infection in patients with spinal cord injury
Shuiling XU ; Min GU ; Xiu YIN ; Yunhai YAO ; Zhiliang YU
Chinese Journal of Physical Medicine and Rehabilitation 2003;0(08):-
Objective To investigate the influence of intermittent catheterization methods on the urinarytract infection in patients with spinal cord injury. Methods Thirty-one cases of spinal cord injury and urinarytract infection were recruited. By use of intermittent catheterization, the bladder function was trained. Bacteria cul-ture and identification of in urine from the patients were conducted before the training (indwelling catheterization)and after 15 or 30 days of intermittent catheterization. Results The rates of urinary tract infection (colony count≥1?10~5cfu/ml) after 15 or 30 days of intermittent catheterization were 38. 7% and 35. 5%, respectively, whichwere significantly lower than those of indwelling catheterization (100%), P
4.Clinical analysis about 5 cases of actinomycete keratitis
Xiao-Tang YIN ; Shi-Yun LUO ; Ran LI ; Shi-Jing DENG ; Zhi-Qun WANG ; Xiu-Ying JIN ; Xu-Guang SUN ;
Ophthalmology in China 1993;0(03):-
Objective To analyze clinical diagnosis and management of 5 patients with actinomycete keratitis.Design Retro- spective case series.Participants 5 patients (5 eyes) with actinomycete keratitis.Methods The clinical features and microbiologic da- ta of 5 culture-proven cases of actinomycete keratitis recorded between October 2004 to March 2006 were analyzed.Main Outcome Measures clinical characteristics,isolations identification,drug susceptibility test and treatments.Results All patients were males and farmers.Of the 5 cases presented in this study,4 cases were followed by minor trauma as a predominant risk factor,and were pre- sented by a chronic progressive corneal ulcer with a wreath pattern of infiltrate.The diagnosis of all cases was based on laboratory in- vestigations,by which 4 cases of nocardia and one case of streptomyce were identified.A variable drug sensitivities were presented in nocardia isolates,which including TMP-SMZ,amicasin,gentamicin and fluorine-quinolones.Conclusions Nocardia keratitis is mainly followed by a minor trauma.It is identified predominantly by laboratory investigations.Tropical and systemically sensitive biotic are the initial choice,while debridement and amnionic transplantation could be an effective alternative.
5.Expression of B lymphocyte stimulator in peripheral blood mononuclear cells in individuals with systemic lupus erythematosus and the role of interferon-? on it's expression
Yu-Jin YE ; Han-Shi XU ; Liu-Qin LIANG ; Pei-Da YIN ; Xiu-Yan YANG ; Zhong-Ping ZHAN ; Fan LIAN ;
Chinese Journal of Rheumatology 2003;0(10):-
Objective To determine the expression of membrane-bound B lymphocyte stimulator (BLyS) protein and its mRNA in vitro of peripheral blood mononuclear cells (PBMCs) from individuals with systemic lupus erythematosus (SLE),and to investigate the role of interferon-?(IFN-?) on the expression of BLyS.Methods PBMCs were obtained from 25 SLE patients (mean age of 31+14) and 20 healthy volunteers (mean age of 28?10).They were randomized into IFN-?(5 ng/ml) group and control group.PBMCs were col- lected at 0,6,12 and 24 h for BLyS mRNA assessment using semi-quantitative reverse transcription-PCR (RT-PCR).PBMCs were also collected at 72 h for membrane-bound BLyS protein detection using flow cy- tometry (FACS) and direct immunofluorescence.Results①The expression of BLyS mRNA and membrane- bound protein in PBMCs was significantly higher in individuals with SLE compared with healthy controls (P<0.05);②IFN-?enhanced BLyS mRNA expression in PBMCs in both healthy controls and SLE patients,with the greatest effect at 6 h (stimulated vs unstimulated,0.42?0.19 vs 0.25?0.14,P<0.01;0.59?0.28 vs 0.44?0.21,P<0.01 );③IFN-?also increased the expression of membrane-bound BLyS protein in both healthy con- trols and individuals with SLE (FACs,mean fluorescence intensity,4.5+3.0 vs 3.7~2.6,P
6.Effect of different cytokine combinations on the expression of CD49d and CXCR4 and ex vivo expansion of umbilical cord blood mononuclear cells.
Ping MAO ; Li XU ; Wen-Jian MO ; Yi YIN ; Yan-Li XU ; Xiu-Mei LIN
Journal of Experimental Hematology 2006;14(2):318-321
This study was purposed to explore the effect of different cytokine combinations on the expansion of the mononuclear cells drived from umbilical cord blood (CB) ex vivo and expression of CXCR4 and CD49d on CD34+ cells after expansion. Human fresh CB mononuclear cells were cultured in serum-free and stroma-free medium containing different combinations of cytokine for 7 days. At day o and 7, the total cells were counted, CD34+ cells and CD34+CXCR4+, CD34+CD49d+ cells were assayed by flow cytometry, and CFU were determined. According to the different combinations of cytokine, experiments were divided into four groups: control, SF group (SCF + FL), SFT group (SCF + FL + TPO) and SFT6 group (SCF + FL + TPO + IL-6). The results showed that the SF (SF group) combination supported only low expansion of total cells, CD34+ cells and CFU. The addition of TPO in SF group restored UCB stem/progenitors expansion to a higher level than that in SF group, while there was no difference between groups SFT and SFT6 (P > 0.05). The cytokine combinations in groups SF, SFT and SFT6 all could upregulate the expression levels of CD49d and CXCR4 on expanded cord blood CD34+ cells, but there were no significant differences between groups SF, SFT and SFT6 (P > 0.05). It is concluded that SCF + FL has no strong synergistic effects on primitive hematopoietic cells. TPO plays an important role in enhancing expansion of umbilical cord blood hematopoietic cells, while IL-6 only shows a neutral effect on it. SCF + FL + TPO combination not only promotes progenitor cells expansion but also upregulates the expression of CD49d and CXCR4 on CD34+ cells from cord blood.
Antigens, CD34
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biosynthesis
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genetics
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Cytokines
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pharmacology
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Drug Synergism
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Fetal Blood
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cytology
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Humans
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Integrin alpha4
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biosynthesis
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genetics
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Leukocytes, Mononuclear
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cytology
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Membrane Proteins
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pharmacology
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Receptors, CXCR4
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biosynthesis
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genetics
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Stem Cell Factor
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pharmacology
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Thrombopoietin
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pharmacology
7.Establishment of the rabbit VX2 tumor as a model for pyriform sinus carcinoma and observation of its biological features.
Na SHEN ; Hai-tao WU ; Xiu-yin XU ; Jian-zhong WANG ; Liang ZHOU
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2009;44(3):251-254
OBJECTIVETo establish the rabbit VX2 tumor as a model for pyriform sinus carcinoma and to observe its biological features.
METHODSVX2 tumor was implanted into the pyriform sinus of 15 rabbits by direct laryngoscope. Fifteen rabbits were randomized into 3 groups (average of 5 rabbits per group). Observation of the tumor growth and evaluation of the histopathological characterization were taken on one group each at the of time 14, 21 and 28 days after transplantation respectively.
RESULTSTumors were found grown in the pyriform sinus of all 15 rabbits with a success implantation rate of 100%. Dysphagia, body weight loss, rhinorrhea and short of breath appeared in the rabbits 28 days after transplantation. The metastasis rates of deep cervical were 100% in all three groups. The metastasis rates of submandibular lymph nodes were 3/5, 4/5 and 5/5 in 14-day, 21-day and 28-day group respectively. The metastasis rates of paratracheal lymph nodes were 0, 4/5 and 5/5 in 14-day, 21-day and 28-day group respectively. There were opposite side lymph node metastasis of deep cervical, submandibular and paratracheal in 4, 3 and 5 rabbits on 14, 21 and 28 days after transplantation respectively. The median diameter for deep cervical, submandibular and paratracheal neck lymph nodes were 1.50, 0.60 and 0.0 cm in 14 days; 1.60, 0.80 and 0.50 cm in 21 days; 1.80, 0.8 and 0.65 cm in 28 days (P > 0.05).
CONCLUSIONSThe animal model for pyriform sinus carcinoma is established successfully. The metastasis of deep cervical lymph node could be induced from day 14 after VX2 transplantation.
Animals ; Disease Models, Animal ; Hypopharyngeal Neoplasms ; pathology ; Lymphatic Metastasis ; pathology ; Neoplasm Transplantation ; methods ; Pyriform Sinus ; pathology ; Rabbits
8.Methylation of FHIT gene promoter region in DNA from plasma of patients with myelodysplastic syndromes and demethylating effect of decitabine.
Yin-Fen DENG ; Lei ZHANG ; Xiu-Qun ZHANG ; Ming-Qiu HU ; Dan DAI ; Xue-Zhong ZHANG ; Yan-Li XU
Journal of Experimental Hematology 2012;20(5):1144-1148
This study was aimed to detect the methylation status of FHIT gene promoter region in the DNA from plasma of patients with myelodysplastic syndrome (MDS), and to investigate the demethylating effect of decitabine. Methylation-specific PCR method was used to detect the methylation status of FHIT gene promoter region in the DNA from plasma of 4 patients with MDS before and after treatment with decitabine plus semis CAG therapy (among them, 1 case of newly diagnosed MDS, 3 cases progressed into acute leukemia). The results indicated that 3 cases were found to have an increased methylation in the promoter region. After treatment with decitabine plus semis CAG, increased methylation was reversed in 2 cases. In 4 cases, 2 cases displayed clinical response. It is concluded that FHIT gene hypermethylation is associated with MDS pathogenesis. Decitabine has demethylating effect on the FHIT gene hypermethylation of plasma from MDS patients. Detecting the methylation status of FHIT gene in DNA from plasma may play a role in MDS auxiliary diagnosis or prognosis.
Acid Anhydride Hydrolases
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genetics
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Adult
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Aged
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Azacitidine
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analogs & derivatives
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therapeutic use
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DNA
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blood
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DNA Methylation
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Female
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Humans
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Male
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Middle Aged
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Myelodysplastic Syndromes
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blood
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drug therapy
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Neoplasm Proteins
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genetics
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Promoter Regions, Genetic
9.Expression and significance of cytokeratins in skin adenexal tumor.
Xin-Gong LI ; Li WEN ; Jing FU ; Zhi-Xiu XU ; Shu-Mei LIU ; Hong GAO ; Xi-Yin SUN ; Xiao-Qiu ZHOU
Chinese Journal of Pathology 2005;34(11):742-743
Adenoma, Sweat Gland
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metabolism
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Diagnosis, Differential
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Humans
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Keratin-14
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metabolism
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Keratin-17
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metabolism
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Keratin-18
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metabolism
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Keratin-7
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metabolism
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Keratins
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metabolism
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Papilloma
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metabolism
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Sebaceous Gland Neoplasms
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metabolism
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Skin Neoplasms
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metabolism
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Sweat Gland Neoplasms
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metabolism