1.The correlation and clinic value of p53,p16,PCNA protein expressions in patients with esophageal carcinoma
Zhong-Ming WANG ; Xiu-Cui LI ; Gui-Rong LIU ; Yong-Mei SUN ; Chun-Luan YUAN ;
Cancer Research and Clinic 2006;0(10):-
Objective To study the expression of p53,p16,PCNA protein in esophageal carcinoma and its relationship to sexual distinction,the location of disease,the biological level,the depth of invasion and lymph node metastasis.Methods 118 patients with esophageal carcinoma were included in the study,all of them were treated for the first time.p53,p16 and PCNA protein in the 118 cases of esophageal carcinoma were detected by immunohistochemical assay(SP technique). Results The positive expression of p53, p16, PCNA protein in 118 patients was 80 %(92/118),42%(50/118)and 97%(115/118),respectively.The positive expression of p53,PCNA protein were irrelated to the sexual distinction,the location of disease,the biological level,the depth of invasion and the lymph node metastasis.The loss of p16 was significantly related to the depth of invasion and the lymph node metastasis(P
3.Studies on the chemical constituents of a fungus producing perylenequinones.
Yun-xiu SHEN ; Wei-zhong LIU ; Xian-guo RONG ; Yi-hua SUN
Acta Pharmaceutica Sinica 2003;38(11):834-837
AIMTo study the chemical constituents in the mycelia of Hypomyces sp..
METHODSSilica gel column chromatography was employed for the isolation and purification. Chemical and spectral methods were used to determine the structures of the isolated compounds.
RESULTSTwo compounds were isolated and identified as: hypomycin C (I) and hypomycin D (II).
CONCLUSIONCompounds I and II are new compounds.
Chromatography, Gel ; methods ; Fermentation ; Hypocreales ; chemistry ; Molecular Conformation ; Molecular Structure ; Mycelium ; chemistry ; Perylene ; analogs & derivatives ; chemistry ; isolation & purification ; Quinones ; chemistry ; isolation & purification
4.Homocysteine promotes endothelial cells to express macrophage inflammatory protein-1alpha.
Shu-xiu WANG ; Fei-yan ZOU ; Zhong-duan DENG ; Zhi-ling QU ; Juan NI ; Qiu-rong RUAN
Chinese Journal of Pathology 2005;34(7):425-426
Cells, Cultured
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Chemokine CCL4
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Chemotaxis, Leukocyte
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drug effects
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Endothelial Cells
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cytology
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metabolism
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Homocysteine
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pharmacology
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Humans
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Macrophage Inflammatory Proteins
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biosynthesis
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genetics
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Monocytes
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physiology
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RNA, Messenger
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biosynthesis
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genetics
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Umbilical Veins
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cytology
5.Combination of EMPs and BMSCs in promoting regeneration of periodontal tissue
zhong-chen, SONG ; rong, SHU ; yu-feng, XIE ; xiu-li, ZHANG ; bin, ZHANG ; ai-mei, SONG ; chao-lun, LI
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(06):-
Objective To evaluate the feasibility of reconstructing horizontal periodontal bone defects by tissue engineering based on bone marrow stromal cells(BMSCs)as seed cells and enamel matrix proteins(EMPs)as growth factors. Methods Two healthy rhesus monkeys were selected, and BMSCs were isolated from iliac marrow and serial subcultivation was conducted. The cells of induced BMSCs at passage 3 were harvested and mixed with Bio-oss collagen. The models of horizontal periodontal bone defects were established surgically in each buccal side of the posterior teeth, and were divided into four groups (blank control group, material group, cells/material group and cells/material/EMPs group). The histological and Micro-CT observation were carried out 8 weeks later. Results In the blank control group, the defects were filled with fibrous connective tissue. There was newly-formed alveolar bone in the material group. In the cells/material group, periodontal regeneration could be observed, while the newly-formed cementum was irregular and less in quantity. In the cells/material/EMPs group, the amount of newly-formed alveolar bone was larger, and the newly-formed cementum was continuous and regular. Conclusion The tissue engineering technique of BMSCs as seed cells in combination with EMPs induction can significantly promote the regeneration of periodontal tissue.
6.The clinical and mammographic features of plasma cell mastitis
Xiu-Rong WU ; Shan ZHONG ; Yu-Bin LIN ; Yu-Fan HUANG ; Xiao-Hua LUO ; Xu-Ming YU ; Xin-Yi WU ;
Chinese Journal of Radiology 2001;0(05):-
Objective To investigate the clinical and mammographic features of plasma cell mastitis.Methods Twenty-five patients(28 lesions)with histologically confirmed plasma cell mastitis, aged from 26 to 70 years(mean age 41 years),were examined with X-ray mammography.The clinical manifestations and imaging features were retrospectively reviewed.Results No case was in lactation.The painful irregular masses,ranged from 1.3 to 8cm in size,were found in 22 patients,while 3 patients with acute episode.Recurrent episodes of breast masses were noted in 4 patients.Based on the mammographic appearances,the plasma cell mastitis were classified as the following four types:inflammation-like type (2/28),ductal ectasia type(3/28),focal infiltration type(10/28)and nodular type(13/28).The valuable radiogyaphic signs:(1)An asymmetrically increased density along the lactiferous duct with a flame-like appearance,inhomogeneous low density tubular structures and scattered stick-shape calcifications.(2) Architectural distortion and oil cysts formation in adjacent area,(3)Subareolar ductal ectasia.Conclusions The clinical and mammographic characteristics of plasma cell mastitis are critical to avoiding unnecessary surgery.Histopathological result is needed for the diagnosis in patients highly suspected of malignancy.
7.Keshan disease survey and analysis on electrocardiography in endemic area of Shandong province in 2008
Wen-ming, ZHANG ; You-zhong, XIANG ; Xiu-hong, WANG ; Lin, WANG ; Shu-liang, SONG ; Fu-rong, QU
Chinese Journal of Endemiology 2010;29(1):96-97
Objective To observe the electrocardiogram changes of threatened crowds in Keshan disease (KSD) endemic area in Shandong province. Methods In 2008,inhabitants from 21 villages of Zoucheng,Sishui,Tengzhou, Yishui, Pingyi, Wulian, Juxian and Qingzhou regions were selected as subjects undergoing electrocardiogram. No less than 100 people were chosen from each village and the examination rate was not lower than 85%. Results Among the 3378 inhabitants investigated,460 cases showed abnormal electrocardiogram and the total incidence of abnormal electrocardiogram was 13.62% (460/3378). The relatively high incidence was T-wave changes,QRS low voltage and ST-T changes,the detection rate being respectively 2.69% (91/3378), 1.92% (65/3378) and 1.72% (41/3378). The highest incidence of abnormal electrocardiogram (26.76%,55/213),the intermediate(21.50%,43/200) and the lowest(5.50%,12/218) was respectively found in Pingyi,Qingzhou and Sishui. Conclusions The threatened crowds in KSD endemic area in Shandong province are still in a state of high abnormal electrocardiogram detection,and electrocardiogram is of great value in the evaluation of KSD patients.
8.Effect of IL-15 on the proliferation, differentiation and anti-apoptosis of CD34+ cells in patients with MDS.
Ming-Zheng CHENG ; Zhong-Lu YE ; Kang-Rong CAI ; Xiu-Lan HUANG ; Ri-Ling CHENG ; Han-Rong CHEN
Journal of Experimental Hematology 2005;13(4):620-623
To study the effect of interleukin-15 (IL-15) on the proliferation, differentiation and apoptosis of MDS CD34(+) cells, CD34(+) cells of high enrichment were separated by MACS system, and cultured in liquid media with different concentration of IL-15 in treated group and without IL-15 in the control group. Apoptosis of hematopoietic precursors was assayed by propidium iodine staining and cell by FCM, and the other MDS CD34(+) cells were stained by cytochemical staining after culture. The results showed that after culture with IL-15 the proliferation and differentiation of MDS CD34(+) cells were obviously promoted. It was found the every lineage of mature cells developed, the expressions of cell surface antigens CD71, CD33 and CD19 all increased in the MDS CD34(+) cell treated with IL-15. It is suggested that IL-15 stimulates the proliferation and differentiation of MDS CD34(+) cells, and partly shows anti-apoptosis effects which may be applicable to the therapy MDS.
Antigens, CD
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immunology
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Antigens, CD19
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immunology
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Antigens, CD34
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immunology
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Antigens, Differentiation, Myelomonocytic
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immunology
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Apoptosis
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drug effects
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Bone Marrow Cells
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drug effects
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immunology
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pathology
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Cell Cycle
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drug effects
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Cell Differentiation
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drug effects
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Cell Proliferation
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drug effects
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Cells, Cultured
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Flow Cytometry
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Humans
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Interleukin-15
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pharmacology
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Microscopy, Fluorescence
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Myelodysplastic Syndromes
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blood
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immunology
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pathology
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Receptors, Transferrin
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immunology
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Sialic Acid Binding Ig-like Lectin 3
9.Ultrastructural and immunohistochemical studies on Trichomonas vaginalis adhering to and phagocytizing genitourinary epithelial cells.
Wen-lie CHEN ; Jin-fu CHEN ; Xiu-rong ZHONG ; Ping LIANG ; Wei LIN
Chinese Medical Journal 2004;117(3):376-381
BACKGROUNDTrichomonas vaginalis (T. vaginalis) belongs to a common sexually transmitted disease pathogen causing genitourinary trichomoniasis in both sexes. We investigated the pathogenetic mechanism of genitourinary trichomoniasis.
METHODSCultured T. vaginalis bodies were injected into the vaginas of rats, or incubated with genitourinary epithelial cells of female subjects, male subjects, and sperm. The ultrastructural and microscopic changes were observed via transmission and scanning electron microscopy and through microscopic histochemistry.
RESULTSGroups of T. vaginalis adhered to PAS positive columnar cells at the surface of stratified epithelium in the middle and upper portions of the vaginas. They also traversed under these cells. The parasites were shown to be PAS, cathepsin D, and actin positive, and they could release hydrolase into the cytoplasm of adhered epithelial cells. In the amebiform T. vaginalis, microfilaments were arranged into reticular formation. Similar phenomena were found during the interaction of T. vaginalis with host cells, both in vitro and in vivo. Usually several protozoa adhered to an epithelial cell and formed polymorphic pseudopodia or surface invaginations to surround and phagocytize the microvilli or other parts of the epithelial cytoplasm. Adhesion and phagocytosis of sperm by the protozoa occurred at 15 - 30 minutes of incubation. Digestion of sperm was found at 45 - 75 minutes and was complete at 90 - 105 minutes.
CONCLUSIONST. vaginalis tends to parasitize at the fornix of the vagina, because this is the site where columnar cells are rich in mucinogen granules and their microvilli are helpful for adhesion and nibbling. T. vaginalis possesses some invading and attacking abilities. Shape change, canalization, encystation, phagocytosis, digestion, the cell coat, cytoskeleton, and lysosome all play important roles in the process of adhesion. They have two methods of phagocytosis: nibbling and ingestion. Genitourinary epithelium may be injured directly by the digestive action of hydrolases, phagocytosis, and the mechanical action of pseudopodia.
Animals ; Cell Adhesion ; physiology ; Cells, Cultured ; Epithelial Cells ; physiology ; Humans ; Hydrolases ; metabolism ; Immunohistochemistry ; Male ; Phagocytosis ; physiology ; Rats ; Rats, Sprague-Dawley ; Trichomonas vaginalis ; metabolism ; ultrastructure ; Urogenital System ; cytology
10.Levels and clinic significance of serum soluble Fas and soluble Fas ligand in coal workers' pneumoconiosis.
Bao-jun YUAN ; Zhi-zhong LIU ; Xiu-rong DING ; Ji-min ZOU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(2):96-98
OBJECTIVETo investigate the changes of levels and clinic significance of serum soluble Fas (sFas) and soluble FasL (sFasL) in coal workers' pneumonoconiosis.
METHODSSerum levels of sFas and sFasL were determined in 52 patients with silicosis, 57 coal workers' pneumonoconiosis, 46 healthy underground coal workers' (the underground control group) and 40 healthy volunteers working on the ground (the ground control group) with a sandwich ELISA.
RESULTSCompared to the underground control and the ground control group, the serum levels of sFas and sFasL in the patients with silicosis and the coal workers' pneumonoconiosis were significantly higher (P < 0.01). Serum levels of sFas and sFasL in the underground control group were significantly higher than those in the ground control group (P < 0.01); Serum sFas levels in coal workers' pneumonoconiosis was significantly higher than those in the patients with silicosis (P < 0.01). Although the serum sFasL levels was also increased, there was no significant difference (P > 0.05). In the patients with silicosis and the coal workers' pneumonoconiosis patients, the serum sFas levels in Phase I patients combined with emphysema and simple Phase II + III patients were significantly higher than those in simple Phase I patients (P < 0.01). There was no significant difference in the serum sFasL levels among various groups with different parameters of pneumonoconiosis. In the patients with silicosis and the coal workers' pneumonoconiosis, serum levels of sFas and sFasL were not significantly altered among different duration of exposure to dusts. There was no correlation between serum levels of sFas and sFasL in the patients with silicosis while there was a slightly positive correlation between sFas and sFasL levels in the coal workers' pneumonoconiosis (r = 0.479, P < 0.05).
CONCLUSIONIn the patients with silicosis and the coal workers' pneumonoconiosis, the serum levels of sFas and sFasL are abnormal and associated with the development of the pneumonoconiosis. The changes of serum sFas levels may indicate the development and progression of the pneumonoconiosis. The detection of the serum sFas level may be used in the differential diagnosis for the silicosis and the coal worker's pneumonoconiosis.
Adult ; Aged ; Coal Mining ; Enzyme-Linked Immunosorbent Assay ; Fas Ligand Protein ; blood ; Female ; Humans ; Male ; Middle Aged ; Pneumoconiosis ; blood ; Silicosis ; blood ; fas Receptor ; blood