1.Cloning and sequence analysis of MYB transcriptional regulator SmP gene of Saussurea medusa Maxim.
Zhi-Ping JIN ; De-Xiu ZHAO ; Chuan-Ling QIAO ; Wen-Quan QU ; Ya-Qiong CHEN ; Chun-Xiang FU
Chinese Journal of Biotechnology 2003;19(3):368-371
A full-length cDNA encoding a MYB-related regulatory gene was isolated from a cDNA library prepared from mRNAs of the red line callus of S. medusa by TD-PCR. The cDNA, designated SmP, is 969 nucleotides long and has an open reading frame of 771 bp with a deduced amino acid sequence of 256 residues. The putative protein of SmP has two typical conversed R2R3-Myb DNA-binding domains in N-terminal and displays a rather high degree of similarity to OsMYB from rice and LBMI from tobacco, showing 73% and 70% identity within the DNA-binding domains. However, the C-terminal domain of the SmP protein does not show obvious similarity to any other known protein sequence. It is rich in hydrophilic amino acids, especially in serine residues (18.38%), partly organized in homopolymeric stretches, a feature often found in activation domain of transcription factors.
Amino Acid Sequence
;
Cloning, Molecular
;
Molecular Sequence Data
;
Phylogeny
;
Plant Proteins
;
chemistry
;
classification
;
genetics
;
metabolism
;
Polymerase Chain Reaction
;
Saussurea
;
classification
;
genetics
;
metabolism
;
Sequence Homology, Amino Acid
;
Transcription Factors
;
chemistry
;
genetics
;
metabolism
2.Effects of sophoridine on the growth and expressions of p53 and vascular endothelial growth factor of transplanted solid tumor SW480 in nude mice.
Qi-rui WANG ; Chun-hua LI ; Xiu-qiong FU ; Ya-wei LIU ; Jing TANG ; Qin FAN ; Xue-gang SUN
Journal of Southern Medical University 2010;30(7):1593-1596
OBJECTIVETo study the effects of sophoridine on the transplanted solid tumor SW480 and the expression of p53 and vascular endothelial growth factor (VEGF) in the tumor in nude mice.
METHODSThe nude mouse model bearing transplanted solid tumor SW480 was established, and the changes in the volume and weight of the tumor were determined after treatment of the mice with sophoridine. Western blotting and immunohistochemistry were employed to examine the expressions of p53 and VEGF proteins, respectively, and fluorescence quantitative PCR used to detect their mRNA expressions in the tumor tissue.
RESULTSThe volume and weight of the tumor xenograft in sophoridine group decreased in comparison with those in the control group. Sophoridine treatment resulted in lowered expressions of p53 and VEGF at both the protein and mRNA levels in the tumor explants as compared with the control group, with a tumor inhibition rate of 34.07% in nude mice.
CONCLUSIONSophoridine can inhibit the growth of transplanted solid tumor of human SW480 cell line, the mechanism of which involves the inhibition of p53 and VEGF expression.
Alkaloids ; pharmacology ; Animals ; Cell Line, Tumor ; Colonic Neoplasms ; metabolism ; pathology ; Humans ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Quinolizines ; pharmacology ; RNA, Messenger ; genetics ; Tumor Suppressor Protein p53 ; metabolism ; Vascular Endothelial Growth Factor A ; metabolism ; Xenograft Model Antitumor Assays
3.Identification of metastasis-related microRNAs of hepatocellular carcinoma in hepatocellular carcinoma cell lines by quantitative real time PCR.
Yue ZHAO ; Hu-liang JIA ; Hai-jun ZHOU ; Qiong-zhu DONG ; Li-yun FU ; Zhao-wei YAN ; Jian SUN ; Ning REN ; Qing-hai YE ; Lun-xiu QIN
Chinese Journal of Hepatology 2009;17(7):526-530
OBJECTIVETo identify the metastasis-related miRNAs in hepatocellular carcinoma (HCC) cell lines.
METHODSA qRT-PCR method was established and optimized.
RESULTSAll candidate metastasis associated miRNAs except miR-124a were expressed in high metastasis cell line MHCC97H and low metastasis cell line MHCC97L, while some miRNAs were differentially expressed between liver cancer cell line (HepG2) and hepatic cell line (L02) (P less than 0.05), these miRNAs include: miR-148b (1.96+/-0.51 vs 3.76+/-0.28), miR-9 (-4.38+/-0.86 vs -1.10+/-0.53), miR-30c (8.41+/-0.40 vs 6.82+/-0.29), miR-338 (3.14+/-0.29 vs -2.36+/-0.32), miR-34a (0.71+/-0.40 vs -2.95+/-0.26), Let-7g (-4.07+/-0.55 vs -6.98+/-0.56). miR-148b expression was about 4 times higher than miR-148a [5.46 (IQR 4.25-6.67) vs 1.29 (IQR 0.94-1.64)] in all cell line tested (Z=-5.097, P=3x10(-7)).
CONCLUSIONThis study may help to understand the biological significance of miRNAs in HCC metastasis.
Carcinoma, Hepatocellular ; genetics ; metabolism ; pathology ; Cell Line ; Cell Line, Tumor ; DNA, Complementary ; genetics ; Epithelial Cells ; metabolism ; Humans ; Liver Neoplasms ; genetics ; metabolism ; pathology ; MicroRNAs ; genetics ; metabolism ; Neoplasm Metastasis ; Polymerase Chain Reaction
4.Precision nursing:trend and implication
Mei.R. FU ; li Ya TIAN ; qiong Xian FENG ; Qian LU ; Yan HU ; yan Yan WANG ; ying Xiao JIANG ; ying Xiu HU
Chinese Journal of Nursing 2017;52(10):1273-1275
The purpose of this article is to provide an overview of precision nursing and its implications for education,practice and research.The emphases are on the contributions of nursing practice and research to genomics as well as precision health and precision medicine.Recommendations are elucidated on how to build knowledge and competence of precision nursing within the broad arena of precision health and precision medicine to promote the development of precision nursing.
5.Dingxin recipe ( ) prevents ischemia/reperfusion-induced arrhythmias via up-regulating prohibitin and suppressing inflammatory responses.
Yu-Hua JIA ; Yun-Xian ZHANG ; Li-Jun LI ; Ya-Wei LIU ; Chun-Hua LI ; Xiu-Qiong FU ; Ping ZENG ; Wei-Kang WU ; Xue-Gang SUN
Chinese journal of integrative medicine 2012;18(2):120-129
OBJECTIVETo identify the underlying mechanisms of the protective effects of Dingxin Recipe (: , DXR), a Chinese compound prescription that has been used clinically in China for more than 20 years, on ischemia/reperfusion (I/R)-induced arrhythmias in rat model.
METHODSA total of 30 rats were randomly divided into three groups: sham group, I/R group, and DXR-pretreated I/R (DXR-I/R) group. Rats in the DXR-DXRI/R group were intragastrically administrated with DXR (12.5 g/kg per day) for consecutive 7 days, while rats I/in the sham and I/R groups were administrated with normal saline. Arrhythmias were introduced by I/R and electrocardiograms (ECG) were recorded. Two-dimensional (2-D) polyacrylamide gel electrophoresis and matrix-matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used to identify assisted differentially expressed proteins. Immunohistochemistry, real-time quantitative polymerase chain reaction (RQ-RQPCR), Western blot, and enzyme-linked immunosorbent assay (ELISA) were performed to analyze proteins PCR), obtained in the above experiments.
RESULTSDXR significantly reduced the incidence and mean duration of ventricular tachycardia and ventricular fibrillation and dramatically decreased the mortality, as well as arrhythmia score, compared with those of the I/R group. Among successfully identified proteins, prohibitin (PHB) and heart fatty acid binding protein (hFABP) were up-regulated in DXR-pretreated I/R rats compared with those of the I/R rats. In addition, compared with the I/R group, the level of glutathione (GSH) was elevated accompanied by reduced expressions of interleukin-6 (IL-6) and neutrophil infiltration in I/R rats with DXR pretreatment.
CONCLUSIONSDXR could alleviate I/R-induced arrhythmias, which might be related to increased expression of PHB. The enhanced expression of PHB prevented against the depletion of GSH and consequently inhibited apoptosis of cardiomyocytes. Furthermore, up-regulation of PHB might ameliorate I/R-induced cell death and leakage of hFABP by suppressing neutrophil infiltration and IL-6 expressions.
Animals ; Arrhythmias, Cardiac ; etiology ; prevention & control ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Electrophoresis, Gel, Two-Dimensional ; Fatty Acid Binding Protein 3 ; Fatty Acid-Binding Proteins ; metabolism ; Glutathione ; metabolism ; Heart Ventricles ; drug effects ; metabolism ; pathology ; Immunohistochemistry ; Inflammation ; complications ; metabolism ; pathology ; Interleukin-6 ; metabolism ; Male ; Myocardial Infarction ; complications ; drug therapy ; pathology ; Neutrophil Infiltration ; drug effects ; Peptide Mapping ; Proteomics ; Rats ; Rats, Wistar ; Reperfusion Injury ; complications ; Repressor Proteins ; metabolism ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Spectrophotometry ; Up-Regulation ; drug effects
6.Epidemiologie investigation on murine typhus in Hongta areas of Yuxi city, Yunnan province of China.
Xue-mei LI ; Li-juan ZHANG ; De-rong ZHANG ; Xiu-ping FU ; Kun LI ; Jing-shan ZHANG ; Pei-de CAO ; Bao-rong PU ; Feng-ying WANG ; Yu-qiong SHI ; Chun ZHANG
Chinese Journal of Epidemiology 2008;29(1):5-8
OBJECTIVETo identify epidemic status of murine typhus in Hongta areas of Yuxi city and to provide evidence for control and prevention of the disease.
METHODSSerologic survey was conducted among residents and rodents. Isolation of Rickettsia moseri was performed.
RESULTSThe overall infection rate among general population was 28.92% (96/332) with geometric meantiter (GMT) as 10.83 and there was no difference between males and females (26.71%, 43/161 vs. 30.99%, 53/171, P > 0.05). Significant differences were found between age groups (P < 0.05) with positive rates of 29.63% (8/27), 18.06% (13/72), 39.62% (42/106), 27.50% (22/80) and 23.40% (11/47) among age groups 0-6, 7-18, 19-39, 40-59 and over 60, respectively. The overall rate of infection in mouse was 44.95% (89/198) with GMT as 30.30. Five isolates of R. moseri from mouse specimen, three from fleas plus one case of murine typhus were diagnosed. Rattus norvegicus and Rattus flavipectus were the predominant species of rodent animals (99.49%, 197/198) and Xenopsylla cheopis was the major species of vector (74.26%, 303/408). Flea index and mouse density were 2.06 and 11.13% respectively.
CONCLUSIONHigh infection rates on R. moseri were demonstrated in rodents and residents as well as high risk of murine typhus outbreak might occur in these areas.
Adolescent ; Adult ; Animals ; Child ; Child, Preschool ; China ; epidemiology ; Female ; Humans ; Infant ; Infant, Newborn ; Male ; Mice ; Middle Aged ; Rats ; Rodent Diseases ; epidemiology ; microbiology ; transmission ; Siphonaptera ; microbiology ; Typhus, Endemic Flea-Borne ; epidemiology ; microbiology ; transmission ; Young Adult
7. Total flavonoids from Saussurea involucrata attenuate inflammation in lipopolysaccharide-stimulated RAW264.7 macrophages via modulating p65, c-Jun, and IRF3 signaling pathways
Li-Shan YAN ; Li WANG ; Yu DING ; Jing KONG ; Shuo-Feng ZHANG ; Gan LUO ; Yi ZHANG ; Brian CHENG ; Qing WANG ; Xiu-Qiong FU
Asian Pacific Journal of Tropical Biomedicine 2021;11(6):273-284
Objective: To investigate the anti-inflammatory effects of the total flavonoids from Saussurea involucrata on lipopolysaccharides (LPS)-stimulated murine RAW264.7 macrophages and explore its underlying mechanism of action. Methods: Total flavonoids from Saussurea involucrata were extracted using chromatographic column method. Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. The production of nitric oxide was detected by Griess assay and the release of cytokines (IL-10 and TNF-α) and chemokines (MCP-1, MIP-1a, and CCL5/RANTES) was determined by ELISA to evaluate the anti-inflammatory activity of total flavonoids from Saussurea involucrata. Moreover, nuclear translocation of p65, c-Jun, and IRF3 was detected by immunofluorescence microscopy and Western blotting analysis was performed to determine the expression of related proteins. Results: Total flavonoids extracted from Saussurea involucrata were 751.5 mg/g and the content of rutin was 506.5 mg/g. The production of inflammatory mediators including nitric oxide, cytokines, and chemokines was effectively inhibited by total flavonoids from Saussurea involucrata. Meanwhile, total flavonoids also suppressed the nuclear translocation of p65, c-Jun, and IRF3 in LPS-stimulated RAW264.7 cells. The LPS-induced expression of iNOS and COX-2 was remarkably reduced by treatment with total flavonoids from Saussurea involucrata. Moreover, total flavonoids decreased the expression levels of p-IKKa/β, p-TBK1, p-p38, p-ERK, p-JNK, p-p65, p-c-Jun, and p-IRF3 in LPS-exposed RAW264.7 macrophages. Conclusions: Total flavonoids from Saussurea involucrata potentially inhibit the secretion of pro-inflammatory mediators, which may be related to inhibition of p65, c-Jun, and IRF3 signaling pathways in LPS-stimulated RAW264.7 cells.
8.Clinical Characteristics and Bone Marrow Histopathology Features in Essential Thrombocythaemia Patients with Different Gene Mutation in China.
Xiu-Peng YE ; Rong WANG ; Quan-Gui WANG ; Yan WANG ; Jian-Fu ZHANG ; Chun QIAO ; Hong-Juan LIU ; Ke-Danmu Aierken AI ; Xing-Xing CHAI ; Xing-Yu LU ; Xiao-Qing LIU ; Lang CHEN ; Zheng-Yuan LIU ; Ye-Qiong LI ; Chun-Yu ZHANG ; Fang LI ; Guang-Sheng HE ; Shen BAO
Journal of Experimental Hematology 2020;28(4):1326-1331
OBJECTIVE:
To investigate the clinical characteristics, laboratorial and bone marrow pathological features of primary thrombocytopenia (ET) patients with different mutations of CALR, JAK2 and MPL genes.
METHODS:
The chinical data of 120 cases of ET in Jiangsu provincial people's hospital/ The First Affiliated Hospital of Nanjing Medical University from January 2015 to December 2017 were collected and analyzed, including 76 cases with JAK2 gene mutation, 40 cases with CALR gene mutation, 2 cases with MPL gene mutations, 2 cases without gene mutation.
RESULTS:
Among the ET patients, compared with the JAK2 gene mutation, CALR gene mutation showed statistically significant deareament of white blood cells and hemoglobin (P=0.001, P=0.01) and the male platelets in CALR group showed significant increament (P=0.04). Fourthermore, the average number of megakaryocytes and its cluster numbers in each hight power field of vision showed statistically significant decreament in CALR group as compared with JAK2 group (P=0.001, P=0.001), and thrombotic events in CALR group were signicantly lower than those in JAK2 group (7.5% vs 18.4%) (P=0.03).
CONCLUSION
Mutations of CALR, JAK2 have different clinical characteristics and blood pathological changes of Chinese ET patients, and their clinical significance is worth to explore.
Bone Marrow
;
Calreticulin
;
genetics
;
China
;
Humans
;
Janus Kinase 2
;
genetics
;
Male
;
Mutation
;
Receptors, Thrombopoietin
;
genetics
;
Thrombocythemia, Essential