1.Clinical effect of orthokeratology for juvenile with myopia astigmatism and its effects on corneal endothelial cells
Zi-Xiu, ZHOU ; Shan-Shan, XU ; Sheng-Ping, YI
International Eye Science 2016;16(8):1525-1527
Abstract?AIM:To investigate the clinical effect of orthokeratology for 400 juvenile with myopia astigmatism and its effects on corneal endothelial cells.?METHODS:Four hundred patients(800 eyes), of whom the average age was 11.5 ±2.3 years old, 239 male, 161 female, were divided into two groups: orthokeratology group and spectacles group. Parameters including efficacy data ( uncorrected visual acuity, corneal curvature, axial length and diopter ) and corneal endothelial cell data ( count of endothelial cell, endothelial cell density, fluorescein staining and central corneal thickness) were observed at 1d, 1, 6, 12 and 24mo after wearing.? RESULTS: The visual acuity of spectacles group recovered to normal after wearing, that of orthokeratology group recovered to normal at 1mo after wearing.At 2a after wearing, the corneal curvature, diopter of orthokeratology group decreased significantly (40.09 ±0.31D, 0.23 ±0.06D respectively); while those of spectacles group increased, the differences between the two groups were significant (P<0.05).The axial length of the two groups increased slightly at 1mo after wearing ( P>0.05 ) compared to those before wearing. At 2a after wearing, the axial length of the two groups were 23.96 ± 0.38mm, 26.49±0.88mm respectively (P<0.05).At 2a after wearing, central corneal thickness was 527.33 ± 27.69mm, 526.98±26.89μm(P>0.05).The count of endothelial cell and endothelial cell density both decreased after wearing without significant differences (P>0.05).?CONCLUSION: Orthokeratology has less effect on the corneal endothelial cells, no obvious adverse reactions and can control the prognosis of myopia.
2.Quality assessment of Periplocae Cortex from different habitats by UPLC fingerprint and quantitative analysis.
Li LI ; Xiu-hui E ; Yi HE ; Ping LI ; Shui-ping ZHOU
China Journal of Chinese Materia Medica 2015;40(8):1529-1534
Ultra performance liquid chromatography (UPLC) was employed for simultaneous determination of three components and fingerprint analysis of Periplocae Cortex with gradient elution of mehtanol and water containing 0.1% phosphoric acid as mobile phase. Three components including chlorogenic acid, 4-methoxysalicylaldehyde and periplocoside were well separated under the analytical condition. Seventeen peaks were selected as the common peaks of 30 batches of Periplocae Cortex. The results showed that there is a significant difference in contents of periplocoside between the samples collected from Henan and Shanxi province. Based on the results of three components quantification and fingerprint analysis, hierarchical clustering analysis ( HCA) and principle component analysis (PCA) were used to further prove the differences between two group samples, and the results indicated that quality of Periplocae Cortex from Shanxi was more stable than that from Henan. The established UPLC fingerprint and quantitative analysis methods could be used efficiently in the quality control of Periplocae Cortex, and this study might contribute to the reasonable clinical application.
Benzaldehydes
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analysis
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China
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Chlorogenic Acid
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analysis
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Chromatography, High Pressure Liquid
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methods
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Drugs, Chinese Herbal
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analysis
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Ecosystem
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Periploca
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chemistry
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classification
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growth & development
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Plant Roots
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chemistry
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Quality Control
3.Albumin-coated microbubbles enhance report gene expression
Wenchao OU ; Jiancheng XIU ; Wenyan LAI ; Ping ZENG ; Zhongjiang ZHOU ; Jian LIU ; Yili LIU
Chinese Journal of Pathophysiology 1986;0(04):-
AIM: To investigate the use of therapy ultrasound to enhance nonviral gene delivery. METHODS: Endothelial cells (EC) and vascular smooth muscle cells (VSMC) were cultured in 6-well plates. Plasmid (pcDNA3.1/His/LacZ) with or without microbubbles at the different concentrations was transfected into the cells with the use of ultrasound for 1 min at 2 MHz, 1.8 mechanical index (MI). Additional controls included ultrasound alone, microbubble alone and microbubble plus plasmid. The rate of blue cells and the activities of ?-Gal were measured. In addition, cell viability was detected with different time from 1 to 30 min of ultrasound irradiation and the different concentrations of microbubbles. RESULTS: In the group of ultrasound with microbubble, the rate of blue cells and activity of ?-Gal markedly increased by 60% and 9-fold, respectively. Microbubbles at concentration of 10% led to the highest transfection effect. Ultrasoud exposure at 1 to 30 minute had no cell toxic effects, while microbubbles at the concentration of 50% had significant effect on cell survival. CONCLUSIONS: Albumin-coated microbubbles markedly enhance gene delivery by therapeutic ultrasound-mediated microbubble destruction, which can be used as a safe and practicality vectors in gene therapy.
4.Surveillance on 138,923 perinatal infant chilopalatognatus cleft lip and palate in Zhengzhou.
Xiu-ping ZHOU ; Ling CUI ; Yu-lin CUI ; Hua-feng GUO
Chinese Journal of Epidemiology 2007;28(5):518-518
China
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epidemiology
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Cleft Lip
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epidemiology
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Cleft Palate
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epidemiology
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Female
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Humans
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Infant, Newborn
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Male
5.The Genetic Stability of Replicating HBV Transgenic Mice
Xiang-Ping KONG ; Qing-Zhou WU ; Xian-Rong LUO ; Lian-Mei HU ; Xiu-Mei LI ; Xue-Rui YI ; Ming-Hua TONG ; Jun-Hui ZHOU ; Guang-Ze LIU
China Biotechnology 2006;0(05):-
Objective:To improve the genetic stability of HBV gene in transgenic mice.Methods:HBV transgenic mice were bred by backcross and double cross.The HBV gene expression and replication were studied with real-time PCR,ELISA and chemiluminescence.Results:The HBV transgenic mice have stably bred to 23rd generation.The serum HBsAg level is 4122.31?2044.74IU/ml;The rate of HBV transgenic mice whose serum HBV DNA reach 104~106copies/ml was 93.93%.The HBV replication and expression were improved markedly.There is no difference between male and female mice about serum HBsAg level.Conclusion:After breeding the HBV gene was expressed stably with high-level in transgenic mice.
6.Detection and the antibiotic susceptibility analysis of mycoplasma and chlamydia in urogenital tract Infections of 327 cases patients with tnbal infertility
Yun ZHOU ; Xue-Lan XU ; Chun-Ping WANG ; Ming ZHOU ; Xiu-Hua ZENG
Chinese Journal of Experimental and Clinical Virology 2011;25(3):201-204
Objective To explore the effects of mycoplasma and chlamydia infections on tubal infertilityand to assess the antibiotic susceptibility and resistance of female urogenital, and consequently to guide clinical rational drug use. Methods 327 tubal infertility women as infertility group and 286 healthy pregnant women as control group were randomly selected, detected chlamydia trachomatis ( CT) , ureaplasma urealyticum ( UU) and mycoplasma hominis ( MH) in cervical secretions and drug resistance of UU and MH. Results CT infection rates ( 14. 99% ), UU infection rates (23. 24% ) , UU + MH infection rates (29.05% ) ,CT + UU + MH infection rates(9. 17% )and total infection rates(88.99% )in infertility group is higher than those (order: 2. 80% ,6. 99% , 8. 39% ,4. 55% ,29.02% ) in the control group, comparisons of two groups are statistically significant differences (P < 0.05 ), the susceptibility of UU to roxithromycin (sensitivity is 96. 05% ), josamycin ( sensitivity is 96.05% ), tetracycline ( sensitivity is 82.89%), vibramycin( sensitivity is 92. 11% ) and clarithromycin( sensitivity is 96.05% ) were relatively high and low to ciprofloxacin and acetyl spiramycin. The susceptibility of MH to josamycin ( sensitivity is 95. 83% ) , vibramycin ( sensitivity is 91. 67% ), minocin ( sensitivity is 83.33%) and actinospectacin ( sensitivity is 75. 00% ) were relatively high and low to erythromycin, azithromycin, roxithromycin and clarithromycin. UU + MH was only sensitive to josamycin ( sensitivity is 90. 52% ), high resistance ( 77. 89% -91. 58% ) to erythromycin, azithromycin, acetyl spiramycin, ciprofloxacin, ofloxacin, azithromycin and clarithromycin.Conclusion Infection of CT, UU, MH and tubal infertility have certain relevance, the rates of CT, UU and MH infection in tubal infertility patients higher than fertile people. For many commonantibacterial drugs, UU, MH and UU + MH has strong resistance, the etiology detection and using adapted antibios should be taken seriously in clinical treatment.
7.Characteristics of Surface Electromyography in Maximum Isometric Voluntary Contraction of Ankle Dorsiflexors and Plantarflexors in Healthy Middle to Old People
Meng LUO ; ping Guo ZHOU ; Min XU ; hong Xiu XU
Chinese Journal of Rehabilitation Theory and Practice 2017;23(10):1157-1161
Objective To establish normal reference range of surface electromyography (sEMG) in related myoelectric activity when maximum isometric voluntary contraction of ankle dorsiflexors and plantarflexors in healthy middle to old people. Methods From Septem-ber to December, 2015, a total of 110 healthy people aged 41 to 75 years were divided into 41 to 50 years group, 51 to 60 years group and>60 years group according to age. The integral electrical values were measured in each subject by sEMG technique and analysis system under maximum isometric voluntary contraction of ankle dorsiflexors and plantarflexors. Results and Conclusion Under ankle dorsiflexion and plantar flexion movement modes, no significant difference was found in the integral electrical values of tibialis anterior muscle, long perone-al muscle, short tibial muscle and gastrocnemius among different groups (F<4.545, P>0.05). No significant difference was found in the inte-gral electrical values of different sides and different genders (F<7.028, P>0.05). Normal reference range for integral electrical values was es-tablished under maximum isometric voluntary contraction of ankle dorsiflexors and plantarflexors in healthy people.
8.Effect of different hemopoietic microenvironment on the differentiation of hemopoietic cells from human embryonic stem cells.
Hui-ping ZHAO ; Hai-jun ZHAO ; Ge LIN ; Di ZHOU ; Tian-cheng LIU ; Guang-xiu LU
Journal of Central South University(Medical Sciences) 2007;32(6):992-996
OBJECTIVE:
To observe the inductive efficiency of deriving hematopoietic cells from human embryonic stem (hES) cells co-cultured with human yolk sac stromal cells, fetal liver stromal cells or fetal bone marrow stromal cells,in order to discuss the effect of the different hemopoietic microenvironment on hemopoietic cytogenesis.
METHODS:
We used two-step method to induce the hES cells into the hematopoietic cells. In the first step the hES cells were co-cultured with cytokines by formation of the day 5 embryoid bodies (5d EBs). In the second step the 5d EB cells were induced into the hematopoietic cells by co-culturing with human yolk sac stromal cells, fetal liver stromal cells or fetal bone marrow stromal cells for 10 days. The inductive efficiencies of deriving hematopoietic cells from hES cells co-cultured with the different hemopoietic microenvironment were reflected by the expression levels of flk, CD34 and CD45 antigen.
RESULTS:
Flow cytometry analysis demonstrated that the population of the cells co-cultured with human yolk sac stromal cells contained flk (1.80%+/-0.56%), CD34 (1.30%+/-0.14%) or CD45 (1.05%+/-0.63%) positive cells; the population of the cells co-cultured with human fetal liver stromal cells contained flk (34.00%+/-25.45%), CD34 (38.40%+/-24.80%) or CD45 (72.60%+/-25.70%) positive cells; the population of the cells co-cultured with human fetal bone marrow stromal cells contained flk (2.50%+/-1.48%), CD34 (3.20%+/-0.56%) or CD45 (1.65%+/-0.21%) positive cells. Compared with spontaneous differentiation of EBs, all of the three stromal cells could induce EBs into the hematopoietic cells (P<0.05).
CONCLUSION
The inductive efficiency of deriving hematopoietic cells from EBs co-cultured with human fetal liver stromal cells was higher than EBs co-cultured with human yolk sac stromal cells and fetal bone marrow stromal cells.
Antigens, CD34
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Cell Differentiation
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Cells, Cultured
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Cellular Microenvironment
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Coculture Techniques
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Embryonic Stem Cells
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cytology
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Fetus
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cytology
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Hematopoietic Stem Cells
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cytology
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Humans
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Leukocyte Common Antigens
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Mesenchymal Stem Cells
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cytology
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Stromal Cells
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cytology
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Yolk Sac
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cytology
9.Feasibility study of laparoscopic complicate myomectomy:analysis of 67 cases
Xue-Lan XU ; Juan-Juan ZHANG ; Chun-Ping WANG ; Hai-Yan LIN ; Yun ZHOU ; Li-Xiu LEI ; Shi LIAO ; Guang-Nan LUO ;
Chinese Journal of Obstetrics and Gynecology 2001;0(07):-
Objective To investigate the feasibility of complicate myomectomy.Methods Six- seven patients with complicated uterine myomas undergoing laparoscopic myomectomy were retrospectively analyzed.The myomectomy was done using ureteral infravision imaging system or/and with self-made myoma segregate-stick.Among the total,there are 29 cases of multiple myomas(the number of myomas≥5)and 23 cases of single myoma(the diameter of myoma≥7cm,including 19 cases of intramural myoma,4 cases of subserous myoma),6 cases of myoma of broad ligament of uterus and 9 cases of cervical myoma.Results All cases were performed successfully laparoscopically.No intra-operative laparotomy or complications occurred.The average operating time and blood loss were(114?32)min and(114?78)ml respectively. The average time of hospital stay was 5.1 d.The average operating time and blood loss in the group (including multiple myoma group,intramural myoma group and cervical myoma)were significantly exceeded the other groups(including myoma of broad ligament of uterus group and subserous myoma group). Conclusions Laparoscopic complicate myomectomy can be performed and the operation indication is enlarged using Ureteral Infravision Imaging System.Advancement in surgical instruments and expert operating skills are the key to operation success.
10.Effects of electrical stimulation of lateral hypothalamic area on gastric ischemia-reperfusion injury in rats.
Xiu-Ping ZHOU ; Jian-Fu ZHANG ; Chang-Dong YAN ; Yong-Mei ZHANG
Acta Physiologica Sinica 2002;54(5):435-440
The effects of electrical and chemical stimulation and electrolytic lesion of lateral hypothalamic area (LHA) on gastric ischemia-reperfusion injury (GI-RI) were investigated in rats whose celiac arteries were clamped for 30 min and reperfused for 60 min by removal of the clamp. The results are as follows. (1) Electrical stimulation of LHA could aggravate GI-RI in an intensity-dependent manner by using 0.2, 0.4 or 0.6 mA current respectively. Microinjection of L-glutamic acid into LHA resulted in a similar effect to that of electrical stimulation of LHA on GI-RI. After electrolytic lesion of bilateral LHA, the area of gastric mucosal injury induced by gastric ischemia-reperfusion (GI-R) was smaller than that by electrical stimulation of LHA plus GI-R. (2) Dorsal vagal complex (DVC) lesion or vagotomy could eliminate the effect of electrical stimulation of LHA on GI-RI. (3) Electrical stimulation of LHA increased the content of malondialdehyde (MDA) but decreased the activity of superoxide dismutase (SOD) of ischemia-reperfusion (I-R) gastric mucosa. (4) Electrical stimulation of LHA plus gastric I-R increased gastric juice volume and total acid output, but there were no significant changes in acidity, pepsin activity and gastric barrier mucus. These results indicate that the LHA is an area in the CNS exerting aggravate effects on GI-RI. The DVC and vagus may be involved in the regulative effects of LHA on GI-RI. These effects are associated with increases in gastric mucosal MDA content, gastric juice volume, and total acid output, and a decrease in SOD activity.Acidity, pepsin activity and gastric barrier mucus do not seem to play an important role.
Animals
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Electric Stimulation
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Gastric Mucosa
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blood supply
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metabolism
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pathology
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Hypothalamic Area, Lateral
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metabolism
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Male
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Malondialdehyde
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metabolism
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Rats
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Rats, Sprague-Dawley
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Reperfusion Injury
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metabolism
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pathology
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Superoxide Dismutase
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metabolism