2.Neonatal chondrodysplasia punctata in a case.
Xiu-jing WU ; Li-ping SHI ; Xiao-lu MA
Chinese Journal of Pediatrics 2009;47(3):229-230
3.Analysis of tongue figure features in 990 cases of sexually transmitted and intravenous drug use spread HIV infected population in Xinjiang.
Ying ZHANG ; Jian-Ping MA ; Xiu-Lan MA ; Lin ZENG ; Aihemaiti ABUDUREYIMU ; Jing-Ru LI
Chinese Journal of Integrated Traditional and Western Medicine 2014;34(7):816-818
OBJECTIVETo investigate the tongue manifestation features of sexually transmitted and intravenous drug use spread HIV infected population in Xinjiang.
METHODSRecruited were 990 HIV infected subjects in Xinjiang from May 2011 to March 2012, who were assigned to the intravenous drug use spread HIV infected (498 cases) and the sexually transmitted (492 cases). By using tongue figure shoot combined with analyses of experts, tongue manifestations were analyzed and compared between the sexually transmitted and the intravenous drug use spread from four aspects, i.e., the tongue color, the tongue shape, the fur color, and the fur property.
RESULTSCompared with the sexually transmitted population, red tongue, fissured tongue, yellow fur, thick fur, eroded fur, deficiency of fur fluid were more often seen, showing statistical difference (P < 0.05). Compared with the intravenous drug use spread population, pale tongue, white fur, and thin fur were more often seen, showing statistical difference (P < 0.05).
CONCLUSIONSThe tongue manifestations of the intravenous drug use spread HIV population reflected inner exuberance of evil toxin and heat impairing qi and yin. Compared with the intravenous drug use spread population, the attack of HIV infection was more hiding in the sexually transmitted population, with milder internal injury. Their Wei-qi was not damaged and no obvious change occurred in the tongue figure.
Adolescent ; Adult ; Aged ; Female ; HIV Infections ; diagnosis ; etiology ; pathology ; Humans ; Male ; Medicine, Chinese Traditional ; Middle Aged ; Sexually Transmitted Diseases, Viral ; diagnosis ; pathology ; Substance-Related Disorders ; complications ; diagnosis ; Tongue ; pathology ; Young Adult
4.Research progress in receptors involved in rotavirus infection.
Xin MA ; Dan-Di LI ; Xiu-Ping LI ; Zhao-Jun DUAN
Chinese Journal of Virology 2014;30(3):303-309
Rotaviruses, which are recognized as one of the major etiological agents among infants and young children with diarrhea, consist of three concentric layers of protein capsid with the enclosed double-stranded RNA genome. Rotaviruses infect host cells mainly by identifying the specific receptors on cell surfaces and binding to them. Therefore, receptors are important factors for viruses infecting cells. So far, there have been many receptors found to be involved in rotavirus infection, including sialic acid, integrin, Toll-like receptor, and blood group antigen. This article provides an overview of receptors involved in rotavirus infection.
Animals
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Humans
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Receptors, Virus
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genetics
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metabolism
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Rotavirus
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genetics
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physiology
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Rotavirus Infections
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genetics
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metabolism
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virology
5.Molecular mechanism of Cigu Xiaozhi formula interfering with HSC-T6 cell activation by regulating HIF-1α signaling pathway based on computer aided drug design
Zhen REN ; Shuo YIN ; Ai-di WANG ; Li WANG ; Xiu-ping ZHAO ; Yan-hua MA
Acta Pharmaceutica Sinica 2023;57(10):3049-3058
In this study, we investigated the effect of Cigu Xiaozhi formula on HSC-T6 activity in hypoxic microenvironment based on network pharmacology and computer-aided drug design, and predicted and verified its possible targets and related signaling pathways. The potential active components and targets of Cigu Xiaozhi formula were screened by searching Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP), Encyclopaedia of Traditional Chinese Medicine (ETCM) and Bioinformatics Analysis Tool for Molecular Mechanism of Traditional Chinese Medicine (BATMAN-TCM) databases, and the liver fibrosis related targets retrieved from Gene Cards and Pharm GK database were integrated to obtain the potential targets of Cigu Xiaozhi formula in the treatment of liver fibrosis. GO enrichment analysis and KEGG signaling pathway enrichment analysis were performed on Omic Share platform, and Cytoscape software was used to construct the "potential active ingredient-key target-pathway" network. The active components and target proteins were subjected to molecular docking analysis by Auto Dock software. According to the results of molecular dynamics simulation and binding free energy calculation, the top 5 active components with degree were scored. The active components stigmasterol and
6.Antidiabetic activity of Callicarpa nudiflora extract in type 2 diabetic rats via activation of the AMPK-ACC pathway
Wen-Yu MA ; Le-Ping MA ; Bo YI ; Min ZHANG ; Shi-Xiu FENG ; Li-Ping TIAN
Asian Pacific Journal of Tropical Biomedicine 2019;9(11):456-466
Objective: To evaluate the antidiabetic effect of Callicarpa nudiflora extract in streptozotocin-induced diabetic rats. Methods: The chemical constituents in Callicarpa nudiflora extract were identified by UPLC-Q-TOF-MS. Callicarpa nudiflora extract (0.15 and 0.3 g/kg) was orally administered to streptozotocin-induced diabetic rats for 42 d. The effects of Callicarpa nudiflora extract on body weight, blood glucose, serum insulin, total cholesterol, triglyceride, LDL-C and HDL-C were investigated, and its effect on liver and pancreatic pathology was assessed by histopathological analysis. Moreover, the expression levels of adenosine 5'-monophosphate-activated protein kinase (AMPK), glucose transporter type 4 (GLUT4), phospho-AMPK/AMPK, and p-acetyl-coA carboxylase (P-ACC)/ACC in the skeletal muscles and liver were determined by reverse transcription-polymerase chain reaction, Western blotting, and immunohistochemistry. Results: A total of 34 compounds, including 8 iridoids, 14 phenylpropanoids, and 12 flavonoids, were identified from Callicarpa nudiflora extract. Callicarpa nudiflora extract significantly reduced blood glucose and significantly restored all other biochemical parameters to near normal levels, including serum insulin, total cholesterol, triglyceride, LDL-C, and HDL-C. Callicarpa nudiflora extract improved insulin resistance and reversed the damage in the liver and pancreas caused by diabetes. Furthermore, Callicarpa nudiflora extract increased the expression levels of phospho-AMPK, GLUT4, P-ACC, and insulin receptor substrate-1 and decreased the expression level of PPAR毭 in diabetic rats.Conclusions: Callicarpa nudiflora extract improved oral glucose tolerance, lipid metabolism, insulin resistance, and reversed the diabetes-related damage in the liver and pancreas by activating the AMPK-ACC pathway.
7.Whole genome analysis of human group A rotavirus G9p8 strains in Hebei lulong region, 2009-2011.
Xin MA ; Dan-Di LI ; Yan-Qing GUO ; Jing-Yao XIANG ; Xiu-Ping LI ; Zhao-Jun DUAN
Chinese Journal of Virology 2014;30(2):119-127
Abstract:This study aims to investigate the genetic characteristics of group A rotavirus (GARV) G9P[8] strains from infantile diarrhea samples in Hebei Lulong region from 2009 to 2011. We randomly selected five GARV G9P[8] strains in Hebei Lulong region from 2009 to 2011, amplified the 11 gene fragments of GARVs by RT-PCR, and analyz their full-genome sequences by homology and phylogenetic analysis with DNAStar and MEGA. The nucleotide homology between strains LL11131077 and LL11131083 in 2011 was significantly higher than hat etween them and the other three strains in 2009 and 2010. The G9P[8] GARVs circulating in Hebei Lulong region from 2009 to 2011 elenged to the same genotype as the prevalent G9P[8] GARVs in other parts of the world. However,the two strains in 2011, compared with those in 2009 and 2010, were located in a different sub-branch of the phylogenetic tree and had amino acid mutations at many sites.
China
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Feces
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virology
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Genome, Viral
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Genotype
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Humans
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Molecular Sequence Data
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Phylogeny
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Rotavirus
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classification
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genetics
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isolation & purification
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Rotavirus Infections
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virology
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Viral Proteins
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genetics
8.Double chimerism in recipient by transplantation of two allogeneic MHC-mismatched mouse fetal blood units.
Bai-Jun SHEN ; Xing-Xia LIU ; Xiu-Li JU ; Li-Ping ZHANG ; Huai-Shui HOU ; Xiu-Feng MA ; Qing SHI
Journal of Experimental Hematology 2002;10(3):243-246
We have constituted a mouse model for fetal blood transplantation (FBT) to cross over major histocompatibility complex (MHC) without causing serious GVHD. It seems that full matching at the MHC appears not necessary for FBT, while the nucleated cell dose is critical. Two fetal blood units were combined from different donors to increase the stem/progenitor cell dose so as to explore the possibility of MHC-mismatched allogeneic transplantation. 26 out of 40 mice in mixed FBT group survived in the observation period of 60 days after transplantation without obvious GVHD. Double chimerism was demonstrated by PCR and flow cytometric analysis; and skin transplantation test proved the induction of donor specific immune tolerance. Our data suggest that two MHC-mismatched allogeneic donor fetal blood units could simultaneously engraft and reconstitute immune and hematopoietic system in a mouse model. The result may be beneficial for the expansion of cord blood application and enables more patients to share the advantages of cord blood transplantation.
Animals
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DNA
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biosynthesis
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Female
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Fetal Blood
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immunology
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transplantation
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Graft vs Host Disease
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immunology
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mortality
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H-2 Antigens
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immunology
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Hematopoiesis
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immunology
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Hematopoietic Stem Cell Transplantation
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methods
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Survival Rate
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Transplantation Chimera
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genetics
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immunology
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Transplantation Tolerance
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immunology
9.Application of fluorescence in-situ hybridization and reverse transcription-polymerase chain reaction in molecular diagnosis of Ewing's sarcoma and primitive neuroectodermal tumor.
Yu YANG ; Hua WANG ; Yan-yu WEI ; Yan ZHANG ; Xiao-long MA ; Xiu-ping YANG ; Jie ZHENG
Chinese Journal of Pathology 2006;35(6):328-332
OBJECTIVETo detect tumor specific chromosome translocations and associated fusion transcripts in paraffin-embedded tissue by interphase fluorescence in-situ hybridization (FISH) and reverse transcription polymerase chain reaction (RT-PCR), respectively, and to evaluate their diagnostic values for Ewing's sarcoma/primitive neuroectodermal tumor (ES/PNET).
METHODSNuclei of the tumor cells and total RNA were extracted from 10 cases of ES/PNET. Interphase FISH was utilized to analyze the EWS gene translocation with a dual color, break apart probe (Vysis company). RT-PCR was used to detect t (11; 22) (q24; q12) and t (21; 22) (q22; q12) fusion transcripts.
RESULTSAmong 10 cases of ES/PNET, the EWS-FLI1 fusion transcript was detected in 8 by RT-PCR. EWS-ERG fusion transcript was not detected in any of the cases. EWS gene translocation was found in 9 of 10 cases by FISH.
CONCLUSIONSInterphase FISH and RT-PCR can be reliably applied to paraffin-embedded tissues for molecular diagnosis of ES/PNET. Between the two approaches, interphase FISH provides a more sensitive and stable result.
Adolescent ; Adult ; Child ; Humans ; In Situ Hybridization, Fluorescence ; methods ; Neuroectodermal Tumors, Primitive, Peripheral ; diagnosis ; genetics ; Polymerase Chain Reaction ; Reverse Transcriptase Polymerase Chain Reaction ; methods ; Sarcoma, Ewing ; diagnosis ; genetics
10.Critical flicker frequency for the diagnosis of minimal hepatic encephalopathy.
Yue WANG ; Xiu-jiang SHI ; XiErNaY ABUDUHEILILI ; Xiao-tang FAN ; Hai-lin MA ; Juan FENG ; Jian SUN ; Li-ya A ; Fang-ping HE
Chinese Journal of Hepatology 2013;21(7):546-547
Adult
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Aged
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Aged, 80 and over
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Female
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Hepatic Encephalopathy
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diagnosis
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Humans
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Male
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Middle Aged
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Neuropsychological Tests
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Psychometrics
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methods
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Young Adult