AIM: To observe the effect of compound anisodine injection combined with yi-qi-tong-luo acupuncture, hot compress and massage for blepharospasm.
METHODS: Selected in our hospital, 60 cases (78 eyes) with blepharospasm were randomly divided into two groups, 30 cases 39 eyes in each group. The observation group were treated with compound anisodine injection combined with yi - qi - tong - luo acupuncture treatment, and supplemented by hot compress and massage, while the control group only with compound anisodine injection. We compared the treatment effect of the two groups.
RESULTS: In the observation group, 38 eyes were clinically effective, 1 eye was invalid, 35 eyes marked, the total effective rate was 97%, the markedly effective rate was 90%; in control group, 32 eyes were the clinically effective, 7 eyes invalid, 23 eyes marked, the total effective rate was 82%, the markedly effective rate was 59%; the total effective rate and markedly effective rate of observation group were higher than those of the control group with statistical difference (P<0. 05). The recurrence rates of the two groups were 5% and 34%, respectively, and the recurrence rate of the observation group was significantly lower than that of the control group ( P <0. 05).
CONCLUSION: The compound anisodine injection combined with yi - qi - tong - luo acupuncture, hot compress and massage for blepharospasm, can significantly improve the efficiency of treatment, with higher effective rate and lower recurrence rate, compared with using compound anisodine alone.

Adult ; Aged ; Case-Control Studies ; Female ; Humans ; Leukemia, Myeloid, Acute ; metabolism ; pathology ; Male ; Middle Aged ; NF-kappa B ; metabolism ; Neovascularization, Pathologic ; pathology ; Tryptases ; metabolism ; Vascular Endothelial Growth Factor A ; metabolism ; Young Adult

AIM: To evaluate the effect of probing of lacrimal passage combining injecting of US-produced TobraDex eye ointment for the treatment of chronic dacryocystitis.METHODS: For 127 cases (129 eyes) of chronic dacryocystitis, first the pyoid secretion gathered in the lacrimal sac was dashed out, and some TobraDex was injected in the middle of the lacrimal passage once per day, repeated for several times. The lacrimal passage was probed when the secretion of lacrimal sac disappeared essentially. The lacrimal passage and immit TobraDex eye ointment was used once every two days, repeated for 3-4 times.RESULTS: In 126 cases (128 eyes) the lacrimal passage was dredged. Only one case (1 eye) the youthful patient did not recover for the opening ectopia of lacrimonasal duct.During the 3mo-1a random visit the chronic dacryocystitis did not recrudesce in the cases of lacrimal passage dredging.CONCLUSION: It is simple and safe to use the probing of lacrimal passage combining injecting of US-produced TobraDex eye ointment to treat the chronic dacryocystitis. This method has good curative effects and can keep the normal physiological structure of lacrimal passage. It does not need any expensive medical equipment and cost less, therefore is advisable for patients.

Objective To establish a micellar electrokinetic capillary chromatography method for content determination of geniposide in Biyuanshu Oral Liquid. Methods Acetaminophen was used as an internal standard, and the separation was performed on an uncoated fused silica capillary of 52 cm × 50 μm ID (42 cm effective length) with the separation voltage of 25.0 kV. The running buffer contained 50 mmol/L borax, 100 mmol/L sodium dodecyl sulfate and 15％ acetonitrile (pH=10). The sample was injected by pressure (10 s, 0.5 psi) and detected at 238 nm. Results Geniposide was in good linearity range of 15.02–320.48 μg/mL (r=0.9995). The repeatability (low, medium and high concentration of samples) and intermediate precision assays gave satisfactory RSD values of less than 1.77％and 2.01％, respectively. The average recovery of geniposide in Biyuanshu Oral Liquid was 97.50％ and the RSD was 4.43％. The contents of geniposide determined by micellar electrokinetic capillary chromatography were in accordance with the results of HPLC analysis. Conclusion The method is simple, fast, accurate and precise, which can be used for the content determination of geniposide in Biyuanshu Oral Liquid.

OBJECTIVETo evaluate clinical efficacy of suture anchors in treating acute injuries of medial collateral ligament (MCL) of knee at degree III.

METHODSTwenty-seven patients with degree III acute MCL injuries of knee were treated with suture anchors from January 2007 to June 2011. There were 15 males and 12 females, aged from 19 to 56 (averaged 32.6) years old. The time from injury to operation was 3 to 10 days, averaged 6 days. Symptoms and physical signs before and after treatment were observed, Lysholm scoring were used to evluated clinical efficacy.

RESULTSAll patients were followed up from 16 to 30 months with an average of 21.6 months. The stability of knee joints was good in all patients. Abduction stress test was negative when the knee joint was straightened at 0 degrees and flexed at 30 degrees. The average degree of flexed knee (67.00 +/- 5.80) degrees preoperatively was lower than that of postoperatively (136.50 +/- 6.30) degrees at 1 year. According to Lysholm scoring, preoperative scores ranged from 30 to 43 points, averaged 36.46 +/- 1.48; 1 year after operation ranged from 87 to 100 with an average of 91.50 +/- 3.80 and higher than postoperative. Twenty patients got an excellent results, 5 good and 2 fair.

CONCLUSIONSuture anchors in treating acute injuries of medial collateral ligament of knee at degree III has following advantages: small range of tissue dissection, easy to operate, reliable fixation and less complications.

Acute Disease ; Adult ; Collateral Ligaments ; injuries ; surgery ; Female ; Humans ; Knee Injuries ; surgery ; Male ; Middle Aged ; Suture Anchors

In order to obtain the fungicides with minimal impact on efficiency of mycorrhizal symbiosis, the effect of five fungicides including polyoxins, jinggangmycins, thiophanate methylate, chlorothalonil and carbendazim on the growth of medicinal plant and efficiency of mycorrhizal symbiosis were studied. Pot cultured Glycyrrhiza uralensis was treated with different fungicides with the concentration that commonly used in the field. 60 d after treated with fungicides, infection rate, infection density, biomass indexes, photosyn- thetic index and the content of active component were measured. Experimental results showed that carbendazim had the strongest inhibition on mycorrhizal symbiosis effect. Carbendazim significantly inhibited the mycorrhizal infection rate, significantly suppressed the actual photosynthetic efficiency of G. uralensis and the most indicators of biomass. Polyoxins showed the lowest inhibiting affection. Polyoxins had no significant effect on mycorrhizal infection rate, the actual photosynthetic efficiency of G. uralensis and the most indicators of biomass. The other three fungicides also had an inhibitory effect on efficiency of mycorrhizal symbiosis, and the inhibition degrees were all between polyoxins's and carbendazim's. The author considered that fungicide's inhibition degree on mycorrhizal effect might be related with the species of fungicides, so the author suggested that the farmer should try to choose bio-fungicides like polyoxins.
Fungi ; drug effects ; growth & development ; physiology ; Fungicides, Industrial ; pharmacology ; Glycyrrhiza uralensis ; chemistry ; growth & development ; microbiology ; physiology ; Mycorrhizae ; drug effects ; growth & development ; physiology ; Plant Extracts ; chemistry ; Symbiosis ; drug effects

A capacitively coupled contactless conductivity detector for high performance liquid chromatography was developed. The detector was consisted of a signal generator, a signal amplifier, and a pair of tubular electrodes. A plastic connecting tube following the separation column was threaded through the two tubular electrodes. When the separated components passed the region between two tubular electrodes, they would be sensed. The instrumental parameters of this detector were investigated, including the internal diameter of connecting tube,the frequency and voltage of excitation signal, electrodes length, and the gap between two electrodes. The 0.5 mm of internal diameter of connecting tube, 70 kHz of excitation frequency, 60 V of excitation voltage,10 mm of electrodes length and 1.5 mm of gap between two electrodes were finally found to be the best working conditions for the detector. This HPLC-C4D(capacitively coupled contactless conductivity detector) was applied to the analysis of oleic and linoleic acids in Brucea javanica oil. The chromatographic peak area was linear with concentration for oleic and linoleic acids in 5-1000 μg/mL, and the limit of detection reached 2.5 μg/mL and 1.0 μg/mL,respectively. The results demonstrate that the present method is sensitive and accurate, proving this homemade contactless conductivity detector has a great application potential for pharmaceutical analysis in future development.

Objective To observe the effect of 1％ lidocaine local injection on alleviating 18G venous cannulation pain.Methods 100 patients were randomly divided into two groups(each had 50 cases),local anesthesia group used 1％ lidocaine 0.2 ml local injections firstly,then performed 18 G vein line needle puncture.The control group performed 18G vein line needle puncture directly.The pain was assessed by visual analogue scale method(VAS).Results 25 patients felt no pain,24 felt mild pain,1 felt moderate pain and no patient felt severe pain in local anesthesia group,and the number was 0,8,31 and 11 respectively in sequence in control group,there was significantly difference(Z =-8.136,P ＜0.01).Conclusions 1％ lidocaine local injection can alleviate the pain of 18G vein line needle cannulation.

Objective To establish a method for content determination of total flavones and polysaccharides in Hedysari Radix in different producing areas in Gansu Province.Methods The contents of total flavones and polysaccharides in Hedysari Radix in different producing areas in Gansu Province were determined by ultraviolet spectroscopy with calycosin-7-glucoside and glucose as reference substance, and the wavelength was set at 260 nm and 484 nm.Results The contents were from 2.82 mg/g to 6.79 mg/g for total flavones and from 106.14 mg/g to 746.40 mg/g for total polysaccharides in Hedysari Radix in different producing areas in Gansu Province. The recoveries of total flavones and total polysaccharides were 97.96％ and 102.90％, respectively.Conclusion There was difference in contents of total flavones and polysaccharides of Hedysari Radix in different producing areas in Gansu Province, and the method of using ultraviolet spectroscopy is simple, reproducible, accurate and reliable, which can be preferably used as the method for content determination of total flavones and polysaccharides in Hedysari Radix.

Objective To explore the relationship of central obesity and brain-derived neurotrophic factor (BDNF) expression in skeletal muscle.Methods Fifty healthy kunming mice at the neonatal stage were randomly divided into control group (n =20 cases) and test group (n =30 cases).To obtain the obese models,test group mice were injected with 10％ monosodium glutamate (3 mg · g-1 · d-1)for 5 days at cervical subcutaneous.In addition,mice were removed according to the requirements.Finally,we got 12 mice in each group.We detected body weight of the mice every two weeks,then Lee' s index was calculated.Body temperature was detected per week.At the end of 8th week,the blood specimens was collected from the eyeball to detect the serum lipid using automatic biochemistry analyzer.The perirenal white adipose tissue (WAT) and interscapular brown adipose tissue (BAT) were isolated and weighed.Furthermore,the levels of BDNF in skeletal muscle were investigated through western blotting in order to analyze the relationship between BDNF with central obesity.Results At the 6th weekend,the body weight of test and control groups were (39.71±2.55) and (32.83 ±2.30)g,the Lee's indexes were (365.02 ±3.83) and (337.54±4.10) g1/3 · cm-1,at the 8th weekend,the body weight of test and control groups were (48.12 ± 3.61) and (39.51 ± 3.52)g,the Lee's indexes were (361.93 ± 7.12) and (325.17 ± 6.87)g1/3 · cm-1 Above indexes had significant difference between the two groups (P ＜ 0.05,P ＜ 0.01 or P ＜ 0.001).At the 6th weekend,the temperature of the two groups were (36.30 ± 0.07) and (36.67 ± 0.07) ℃,at the 7th weekend,the temperature of the two groups were (36.40 ±0.08)and (36.79 ± 0.10)℃ respectively,at the 8th weekend,the temperature of the two groups were (36.31 ± 0.09)and(36.80 ± 0.10)℃ respectively.Above indexes had significant difference between the two groups.At the 8th weekend,the main indexes in test and control groups were compared:the total cholesterol were(2.91±0.25) and (1.86 ± 0.51) mmol · L-1,the triglyceride were (1.48 ± 0.62) and (0.81 ±0.23)mmol · L-1,the low-density lipoprotein were (0.37 ± 0.06) and (0.29 ± 0.05) mmol · L-1,the WAT were (0.75 ± 0.08) and (0.24 ± 0.05) g,the BAT were (0.31 ± 0.07) and (0.17 ±-0.01) g,the value of the BAT/WAT were (0.41 ±0.08) and (0.71 ± 0.12),the expression of BDNF were (0.63 ± 0.07) and (0.98 ± 0.06).The differences were statistically significant (P ＜ 0.05,P ＜ 0.01 or P ＜ 0.001).Conclusion Down-regulation of BDNF in skeletal muscle may be involved with central obesity of mice by affecting energy metabolism.