2.In vitro antimicrobial activity of ampicillin-sulbactam,clindamycin and cefoperazone determined by E test
Ying ZHAO ; Ying-Chun XU ; Hui WANG ; Qi-Wen YANG ; Hong-Li SUN ; Xiu-Li XIE ; Min-Jun CHEN ;
Chinese Journal of Infection and Chemotherapy 2007;0(05):-
Objective To investigate the in vitro antimicrobial activity of ampicillin-sulbactam,clindamycin and cefoperazone a- gainst common clinical isolates.Methods MICs of these three antibiotics were determined by E test.Results Ampicillin-sulbae- tam showed inhibition rate of 100% for methicillin susceptible S.aureus (MSSA),E.faecalis,Group A Streptococcus,H. influenzae,penicillin-susceptible S.pneumoniae (PSSP),M.catarrhalis and anaerobes (including 10 strains of Bacteroid spp.,2 strains of P.aches,1 strain of E.lentum,1 strain of Fusobacterium innocuum,and 2 strains of Peptostreptococcus spp.).Ampicillin-sulbactam was active against 86.7% of extended-spectrum?-lactamase non-producing K.pneumoniae (NES- BL-KPN) and 53.3% of non ESBL-producing E.coli (NESBL-ECO).Ampicillin-sulbactam only inhibited 23.3% of A.bau- mannii isolates and 25.0% of E.faecium isolates.For MSSA,anaerobes,PSSP and group A Streptococcus,about 60.0%, 31.2%,30.0% and 10.0% of the isolates were susceptible to clindamycin,respectively.For MSSA,NESBL-ECO and NES- BL-KPN,96.7% to 100% of the isolates were susceptible to cefoperazone.About 40.0% of P.aeruginosa isolates were sus- ceptible to cefoperazone.No A.baumannii isolate was susceptible to cefoperazone.Conclusions The ampicillin-sulbactam has good antimicrobial activity against MSSA,E.faecalis,Group A Streptococcus,NESBL-KPN,H.influenzae,PSSP,M.ca- tarrhalis and anaerobes.In this study,clindamycin is active against 60% of MSSA isolates.Most of other species are relatively resistant to clindamycin.Cefoperazone shows good activity against MSSA,NESBL-ECO,and NESBL-KPN.These three anti- microbial agents can be used as empirical treatment for community-acquired infections.
3.Multidrug-resistant Clinical Enterobacter cloacae Isolates: Analysis of Their ESBLs Gene Types and Molecular Epidemiology
Hong LIU ; Xiao-Fei JIANG ; Fei-Yi RUAN ; Min LI ; Fu-Qi AI ; Yi-Min MA ; Xiu-Hua HONG ; Yuan LV ;
Chinese Journal of Nosocomiology 2006;0(05):-
OBJECTIVE To understand the distribution and epidemiology of ESBLs in ceftazidime or cefotaxime-(resistant) clinical Enterobacter cloacae isolates.METHODS Twenty seven ceftazidime or cefotaxime-resistant(nonrepetitive) E.cloacae were collected from 27 patients hospitalized at the Huashan Hospital,Shanghai.PCR and(sequencing) were performed to understand the distribution of ESBLs in E.cloacae;rep-PCR was(performed) to(understand) the epidemiology of ESBLs in E.cloacae.RESULTS CTX-M-3 like(ESBLs) were the most prevalent in our study(48%);this was the first report of VEB-1-like ESBLs in the member of(Enterobacteriaceae) in China,and the first report of the ESBLs VEB-1-like and CTX-M-3-like in an isolate simultaneously;the majority of(ESBLs) producers exhibited the same rep-PCR pattern,but harbored different ESBLs gene.(CONCLUSIONS) In our study,ESBLs have become prevalent in clinical E.cloacae isolates,and become an important factor of E.cloacae isolates resistant to extended-spectrum beta(-lactams).
4.Association between insulin resistance and cholesteryl ester transfer protein gene polymorphism in type 2 diabetes mellitus.
Tao JIANG ; Xiu-xia SONG ; Min ZHANG ; Wei-hong QI ; Xiao-wei ZHANG
Chinese Journal of Medical Genetics 2005;22(3):298-301
OBJECTIVETo study the association between cholesteryl ester transfer protein (CETP) gene polymorphism and insulin resistance in type 2 diabetes.
METHODSCETP-TaqIB gene was genotyped with polymerase chain reaction-restriction enzyme fragment polymorphism analysis in 108 patients with type 2 diabetes and in 60 normal controls. Plasma lipids, fasting insulin, insulin sensitivity index and insulin resistance index were determined in 108 patients with type 2 diabetes.
RESULTSThe distribution of CETP-TaqIB genotypes and B1B2 allele frequency in the patients with type 2 diabetes were similar to that in general population. High density lipoprotein cholesterol (HDL-C), apolipoprotein A1(apoA1) and insulin sensitivity index (ISI) levels were significantly higher in B2B2 genotype than in B1B1 genotype. Fasting insulin (FINS) and Homeostasis model assessment-insulin resistance (HOMA-IR) levels were significantly lower in B2B2 genotype than in B1B1 genotype. No significant differences in triglyceride (TG), total cholesterol(TC),low density lipoprotein cholesterol (LDL-C), apolipoprotein B (apoB) levels were observed among different CETP-TaqIB genotype groups. By multivariate analysis, the determinants of ISI and HOMA-IR were body mass index (BMI), TC, HDL-C and CETP-TaqIB genotype.
CONCLUSIONThe CETP-TaqIB genotype is independently associated with insulin resistance and lipid metabolism. It may be an important risk factor of insulin resistance in type 2 diabetes.
Adult ; Aged ; Aged, 80 and over ; Cholesterol Ester Transfer Proteins ; genetics ; metabolism ; Diabetes Mellitus, Type 2 ; genetics ; metabolism ; Female ; Gene Frequency ; Genotype ; Humans ; Insulin Resistance ; Lipid Metabolism ; Male ; Middle Aged ; Polymorphism, Genetic ; genetics
5.Transcatheter closure of an aorto-pulmonary septal defect in a case.
Xian-yang ZHU ; Yan JIN ; Xiu-min HAN ; Qi-guang WANG ; Wei QUAN ; Chuan-ju HOU ; Ming WEI ; Yu-wei ZHANG
Chinese Journal of Pediatrics 2004;42(7):551-551
Aorta
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pathology
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surgery
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Aortopulmonary Septal Defect
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therapy
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Balloon Occlusion
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methods
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Catheterization, Swan-Ganz
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methods
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Child
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Female
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Humans
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Pulmonary Artery
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pathology
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surgery
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Treatment Outcome
6.Characteristics of boningmycin induced cellular senescence of human tumor cells.
Xiu-Min ZHANG ; Ning GAO ; Ru-Xian CHEN ; Hong-Zhang XU ; Qi-Yang HE
Acta Pharmaceutica Sinica 2010;45(5):589-594
Cellular senescence is one of the important steps against tumor. This study was to observe the characteristics of boningmycin induced senescence of human tumor cells. MIT method and clone formation assay were used to detect the growth-inhibitory effect. Cellular senescence was detected with senescence-associated beta-galactosidase staining. Cell cycle distribution and accumulation of intracellular reactive oxygen species (ROS) were analyzed with flow cytometry. Protein expression was detected by Western blotting. The results showed that the growth-inhibitory effect of boningmycin was obviously stronger on human oral epithelial carcinoma KB cells than that on non-small cell lung cancer A549 cells. Comparison to the similar action of doxorubicin, that boningmycin induced the features of cellular senescence in both cell lines, its due to the arrest at G2/M phase and an increase of ROS level. The molecular senescence marker P21 increased significantly after boningmycin treatment at a dosage of 0.1 micromol x L(-1), whereas a higher concentration of it induced apoptosis. The results indicated that cellular senescence induced by boningmycin was one of its mechanisms in tumor suppression.
Antibiotics, Antineoplastic
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administration & dosage
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pharmacology
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Apoptosis
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drug effects
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Bleomycin
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administration & dosage
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analogs & derivatives
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pharmacology
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Carcinoma, Non-Small-Cell Lung
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metabolism
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pathology
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Cell Cycle
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drug effects
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Cell Proliferation
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drug effects
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Cellular Senescence
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drug effects
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Cyclin-Dependent Kinase Inhibitor p21
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metabolism
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Dose-Response Relationship, Drug
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Doxorubicin
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pharmacology
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Humans
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KB Cells
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Lung Neoplasms
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metabolism
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pathology
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Poly(ADP-ribose) Polymerases
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metabolism
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Reactive Oxygen Species
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metabolism
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Tumor Suppressor Protein p53
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metabolism
7.Optimal waveband identification for estimation of leaf area index of paddy rice.
Fu-min WANG ; Jing-feng HUANG ; Qi-fa ZHOU ; Xiu-zhen WANG
Journal of Zhejiang University. Science. B 2008;9(12):953-963
The objectives of the study were to select suitable wavebands for rice leaf area index (LAI) estimation using the data acquired over a whole growing season, and to test the efficiency of the selected wavebands by comparing them with feature positions of rice canopy spectra. In this study, the field experiment in 2002 growing season was conducted at the experimental farm of Zhejiang University, Hangzhou, China. Measurements of hyperspectral reflectance (350 approximately 2500 nm) and corresponding LAI were made for a paddy rice canopy throughout the growing season. And three methods were employed to identify the optimal wavebands for paddy rice LAI estimation: correlation coefficient-based method, vegetation index-based method, and stepwise regression method. This research selected 15 wavebands in the region of 350~2 500 nm, which appeared to be the optimal wavebands for the paddy rice LAI estimation. Of the selected wavebands, the most frequently occurring wavebands were centered around 554, 675, 723, and 1 633 nm. They were followed by 444, 524, 576, 594, 804, 849, 974, 1 074, 1 219, 1 510, and 2 194 nm. Most of them made physical sense and had their counterparts in spectral known feature positions, which indicates the promising potential of the 15 selected wavebands for the retrieval of paddy rice LAI.
Oryza
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anatomy & histology
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growth & development
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Plant Leaves
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anatomy & histology
8.Development and validation of HPLC-UV-MS method for the control of four anti-diabetic drugs in suspected counterfeit products.
Xiu-mei DAI ; Ning AN ; Jian-min WU ; Hui-yi LI ; Qi-ming ZHANG
Acta Pharmaceutica Sinica 2010;45(3):347-352
An HPLC-UV method has been developed for the determination of valibose, miglitol, voglibose and acarbose, the four anti-diabetic drugs. The separation was accomplished successfully by using reversed phase chromatography (Prevail carbohydrate column, 250 mm x 4.6 mm, 5 microm) with a gradient acetonitrile-phosphate buffer solution (pH 8.0) at a wavelength of 210 nm. Furthermore, the method of a high-performance liquid chromatography coupled with ESI-MS in positive ionization mode has been established. These two methods were successfully applied to the assay and qualitative detection of four alpha-glucosidase inhibitors in the potential counterfeit anti-diabetic drugs.
1-Deoxynojirimycin
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analogs & derivatives
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analysis
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Acarbose
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analysis
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Chromatography, High Pressure Liquid
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methods
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Chromatography, Reverse-Phase
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Glycoside Hydrolase Inhibitors
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Hypoglycemic Agents
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chemistry
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Inositol
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analogs & derivatives
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analysis
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Spectrometry, Mass, Electrospray Ionization
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methods
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Spectrophotometry, Ultraviolet
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alpha-Glucosidases
;
analysis
9.Analysis of serum insulin-like growth factor-1 and insulin-like growth factor-binding protein-3 in benign prostatic hyperplasia.
Jing-Ping YU ; Xiu-Mei WU ; Jian-Guo CHEN ; Wei-Min LIU ; Qi-Xian YANG
National Journal of Andrology 2003;9(5):341-343
OBJECTIVESTo determine changes of insulin-like growth factor-1 (IGF-1) and IGF binding protein-3 (IGFBP-3) in serum samples from benign prostatic hyperplasia (BPH) patients and to evaluate the value of these molecules as possible etiologic factors for BPH.
METHODSThe serum IGF-1 and IGFBP-3 levels were measured with immunoradiometric assay(IRMA) in 64 cases of BPH and in 30 healthy subjects as controls. The cases of BPH were divided into 3 groups according to the prostate volume(PV). There were 18 cases(PV < or = 30 ml) in group A, 24 cases(PV31 approximately 50 ml) in group B, 22 cases(PV > or = 50 ml) in group C.
RESULTSThere were no statistical differences between the levels of Both IGF-1 and IGFBP-3 in BPH groups and healthy groups (both P > 0.05), but there were statistical differences among three groups of BPH. Both IGF-1 and IGFBP-3 levels in group C of BPH were significantly higher than those in group A (both P < 0.05). A positive correlation between the serum levels of IGF-1 and PV displayed(r = 0.58), as well as IGFBP-3 (r = 0.48).
CONCLUSIONSTogether these observations implicate IGF-1 and IGFBP-3 as important factors during the progression of BPH. It shows the value of non-operation treatment for this disease.
Aged ; Humans ; Insulin-Like Growth Factor Binding Protein 3 ; blood ; Insulin-Like Growth Factor I ; analysis ; Male ; Prostatic Hyperplasia ; blood ; Radioimmunoassay
10.Oncogenic role of Skp2 and p27Kip1 in intraductal proliferative lesions of the breast.
Lv YAN ; Niu YUN ; Ding XIU-MIN ; Xiao XU-QI
Chinese Medical Sciences Journal 2012;27(3):161-166
OBJECTIVETo investigate whether the connection of p27(Kip1) to S-phase kinase-associated protein 2 (Skp2) plays an oncogenic role in intraductal proliferative lesions of the breast.
METHODSHere we investigated the mechanism involved in association of Skp2’s degradation of p27(Kip1) with the breast carcinogenesis by immunohistochemical method through detection of Skp2 and p27(Kip1) protein levels in 120 paraffin-embedded tissues of intraductal proliferative lesions including usual ductal hyperplasia (UDH, n=30), atypical ductal hyperplasia (n=30), flat epithelial atypia (FEA, n=30), and ductal carcinoma in situ (DCIS, n=30). Moreover, the expression status of Skp2 and p27(Kip1) in 30 cases of the normal breast paraffin-embedded tissues were explored.
RESULTSThe DCIS group was with the highest Skp2 level and the lowest p27(Kip1) level, and the UDH group was with the lowest Skp2 level and the highest p27(Kip1) level.Both Skp2 and p27(Kip1) levels in the DCIS group were significantly different from those in the UDH group (all P<0.01).The levels of Skp2 and p27(Kip1) in the FEA group were significantly different from both the DCIS and UDH groups (all P<0.05).p27(Kip1) was negatively correlated with Skp2 in both the UDH group (r=-0.629, P=0.026) and DCIS group (r=-0.893, P=0.000).
CONCLUSIONOverexpression of Skp2 might be the mechanism underlying p27(Kip1) over degradation.
Adult ; Aged ; Breast ; pathology ; Breast Neoplasms ; etiology ; Carcinoma, Intraductal, Noninfiltrating ; etiology ; Cell Proliferation ; Cyclin-Dependent Kinase Inhibitor p27 ; physiology ; Female ; Humans ; Hyperplasia ; Middle Aged ; S-Phase Kinase-Associated Proteins ; physiology