1.Determination of amino acids in Galli Gigerii endothelium corneum by HPLC with pre-column derivatization.
Kun LIANG ; Dan ZHANG ; Ji SHI ; Hong HU ; Yan-Feng XIU ; Tian-Zhu JIA
China Journal of Chinese Materia Medica 2014;39(8):1463-1467
Hydrolytic amino acids were extracted by acid hydrolysis method, then derivatized with phenyl isothiocyanate (PITC). And the samples were analysed by HPLC on an Ultimate Prime C18 (4.6 mm x 250 mm, 5 microm) column with gradient elution of 0.1 mol x L(-1) sodium acetate buffer solution (adjusted to pH 6. 5)-acetonitrile (93:7) (A) and acetonitrile-water (8:2) (B) at a flow rate of 1.0 mL x min(-1). Column temperature was 40 degrees C and the detected wavelength was 254 nm. Amino acids derivative solution remained stable in 36 hours. The response was linear for 16 amino acids with a correlation coefficient r > 0.999 5. The average recoveries were 98.01% -101.8%. The method is reliable with good accuracy and repeatability, which is useful for the determination of amino acids in Galli Gigerii Endothelium Corneum.
Amino Acids
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analysis
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Animals
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Chickens
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Chromatography, High Pressure Liquid
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Chromatography, Reverse-Phase
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Endothelium
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chemistry
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Gizzard, Avian
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chemistry
2.Determination of sulfur dioxide residues in sulfur fumigated Chinese herbs with headspace gas chromatography.
Zheng-Wei JIA ; Bei-Ping MAO ; Shui MIAO ; Xiu-Hong MAO ; Shen JI
Acta Pharmaceutica Sinica 2014;49(2):277-281
This paper aims to establish a method for the determination of sulfur dioxide in sulfur fumigation Chinese herbs. Sample powder and hydrochloric acid solution were isolated by paraffin layer in order to avoid early reactions, with the generation of sulfur dioxide, headspace with airtight needle was used to transfer sulfur dioxide into gas chromatograph, and detected with thermal conductivity detector. The analytical performance was demonstrated by the analysis of 12 herbs, spiked at four concentration levels. In general, the recoveries ranging from 70% to 110%, with relative standard deviations (RSDs) within 15%, were obtained. The limit of detection (LOD) was below 10 mg x kg(-1). Standard addition can be used for low recovery samples. The method is simple, less time-consuming, specific and sensitive. Methods comparison revealed that gas chromatography is better than traditional titration in terms of method operability, accuracy and specificity, showing good application value.
Chromatography, Gas
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methods
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Fumigation
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Limit of Detection
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Plants, Medicinal
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chemistry
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Sulfur
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chemistry
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Sulfur Dioxide
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analysis
3.Analyses of serum free fatty acid composition and other glucose and lipid metabolic parameters in middle and old-aged patients with metabolic syndrome in community
Jia-Qiang LI ; Miao-Ying LI ; Yu-Min LIU ; Ying-Xiu DAI ; Ji ZHENG ; Wen-Bin LIU ;
Chinese Journal of General Practitioners 2003;0(05):-
Objective To explore the relationship between free fatty acid(FFA)composition and other glucose and lipid metabolic parameters in patients with metabolic syndrome(MS)in community. Methods Serum FFA profile was measured with gas chromatography and mass spectrometry in 158 patients with varied metabolic syndrome components(MSC),including 61 with MS and 97 at high-risk for MS,and 43 control subjects,with diagnostic criteria by the International Diabetes Federation(IDF).Results Patients with MS had higher parameters of polyunsaturated fatty acid(PUFA)and n6PUFA,as compared to the patients at high risk and normal subjects(P0.05). Among the high-risk group,those with diabetes had increased linoleic acid,n6PUFA and total fatty acid (TFA),and decreased saturated fatty acid(SFA)/TFA,as compared to those without diabetes(P
4.Clinical and pathological characteristics of septum pellucidum tumor and choice of surgical approaches for its resection.
Lei WANG ; Mao-zhi ZHANG ; Wei ZHANG ; Shang-feng ZHAO ; Ji-zong ZHAO ; Jin-xiu JIA
Chinese Medical Journal 2005;118(10):812-816
BACKGROUNDTumor involving the septum pellucidum is uncommon. Surgery as the main therapeutic procedure for this lesion is a challenge to neurosurgeons. We analyzed the clinical characteristics and pathological features of septum pellucidum tumor in 41 patients and compared the curative effects of frontal transcortical, trans-sulcal and interhemispheric transcallosal approaches.
METHODSClinical characteristics and the pathological features of septum pellucidum tumor were investigated retrospectively in 41 patients. The differences in postoperative residual rates, extents of tumors and resection of normal brain tissues after use of the three approaches in these patients were analyzed statistically.
RESULTSSeptum pellucidum tumor is more likely to attack young or middle-aged persons. The tumor mainly presents itself as a central neurocytoma or cerebral low-grade glioma in pathology and manifests as intracranial hypertension clinically. No difference was found in the extent of tumor resection but significant difference in the extent of normal brain tissue resection and in postoperative disability rate among the three approaches. The transcortical approach brought about the most serious injury to brain tissue and the highest disability rate, Whereas the frontal transcallosal approach the lightest injury and the lowest disability rate. The injury to brain tissue and the disability rate brought about by the front trans-sulcus approach were between the above two approaches.
CONCLUSIONSOperation is still regarded the major treatment for septum pellucidum tumor. Transcallosal and trans-sulcus approaches are fit with the concept of minimally invasive surgery, and transcallosal approach is the first choice for septum pellucidum tumor.
Adolescent ; Adult ; Cerebral Ventricle Neoplasms ; pathology ; surgery ; Child ; Female ; Follow-Up Studies ; Humans ; Male ; Middle Aged ; Septum Pellucidum
5.Relationship between fatigue caused by type 2 diabetes mellitus and 5-HT degradation in skeletal muscle
Yu-xin ZHANG ; Rui ZHANG ; Jing YANG ; Xue-chun SHAN ; Xiu-rui LIANG ; Yi ZHANG ; Fan XU ; Jia-qi JIN ; Jing GUAN ; Ji-hua FU
Acta Pharmaceutica Sinica 2021;56(1):190-200
Fatigue is a common complication of type 2 diabetes mellitus (T2DM). We examined the relationship between T2DM fatigue and the skeletal muscle 5-hydroxytryptamine (5-HT) system. In animal experiments, a T2DM model was established in mice by feeding a high-fat diet with intraperitoneal injection of streptozotocin. The mice were treated with the 5-HT2A receptor antagonist sarpogrelate hydrochloride (SH) and the 5-HT synthesis inhibitor carbidopa (CDP) (separately and in combination). In cell culture experiments, C2C12 cells were stimulated with
6.Simultaneous determination of 56 organochlorine and pyrethroid pesticides in traditional Chinese medicines by GC coupled with dual-tower and dual-column.
Zheng-wei JIA ; Xiu-hong MAO ; Shui MIAO ; Ji-wei LU ; Ke CHEN ; Ke WANG ; Shen JI
Acta Pharmaceutica Sinica 2010;45(3):353-358
The paper is to report the establishment of a method for the determination of multi-residue organochlorine and pyrethroid pesticides in traditional Chinese medicines (TCMs). Fifty-six pesticides were extracted by high-speed homogenization, and then purified through gel permeation chromatography (GPC) and solid phase extraction (SPE) cartridges. The residues were simultaneously identified and quantified by GC-ECD equipped with dual tower, dual column and two micro-ECD detectors. The analytical performance was demonstrated by the analysis of 3 TCMs samples' extracts, spiked at three concentration levels for each pesticide. In general, the recoveries ranging from 70% to 110%, with relative standard deviations (RSDs) better than 15%, were obtained. The limit of detection (LOD) for most of the targeted pesticides tested was below 0.01 mg kg(-1). The method had good extraction efficiency, purification effect and good reproducibility, which could be applied to the determination of organochlorine and pyrethroid pesticide residues in the routine analysis of TCMs.
Chromatography, Gas
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methods
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Chromatography, Gel
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Drug Contamination
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Drugs, Chinese Herbal
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chemistry
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Hydrocarbons, Chlorinated
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analysis
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Limit of Detection
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Pesticide Residues
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analysis
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Pyrethrins
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analysis
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Reproducibility of Results
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Solid Phase Extraction
7.The construction of SCID-Hu IC mice model and application in rAd5HPV16L1-E7 vaccine.
Chang-qin SONG ; Yong LI ; Yi LUAN ; Ji-feng BIAN ; Li ZHAO ; Wei-ming ZHAO ; Ji-hui JIA ; Ya-bin ZHOU ; Mei QI ; Xiu-ping YU
Chinese Journal of Experimental and Clinical Virology 2004;18(3):243-246
OBJECTIVETo construct human-SCID chimeric mice through implantation of mononuclear cells from human cord blood and study the immunoreaction of SCID-Hu IC mice immunized with rAd5HPV16L1-E7 vaccine.
METHODS(1) Experiment groups were injected with the suspension of mononuclear cells from human cord blood through a tail vein; the control ones were injected with non serum RPMI 1640 medium. Eight weeks after implantation, blood was collected and human serum IgG level in the mice were tested, and human CD45, CD3 and CD19 were determined. (2) SCID-Hu IC mice were divided into two groups: in group A the mice were immunized intraperitoneally with rAd5HPV16L1-E7 virus and in group B the mice were immunized through nasal drip with rAd5HPV16L1-E7 virus. At the end of fourth week, the serum specific IgG antibody to rAd5HPV16L1-E7 virus, IFN-gamma in culture medium of spleen lymphocyte and T-lymphocyte propagation were tested.
RESULTS(1) In the experiment groups, the number of mice positive for human IgG was 10/15, the average values of CD45, CD3 and CD19 were (9.39+/-4.21), (3.25+/-3.99) and (1.69+/-0.75), respectively. In the control ones, the human IgG, CD45, CD3 and CD19 were negative. (2) The results in the experiment groups showed that the IFN-gamma and T-lymphocyte stimulated by HPV16 protein were higher than those in the non-stimulated group (P less than 0.05).
CONCLUSION(1) The results indicated that the construction of human-SCID chimaera through the implantation of mononuclear cells from human cord blood into SCID mice was successful. They also indicated that the reconstructed SCID-Hu IC mice has the ability to produce immune response against rAd5HPV16L1-E7 recombinant virus.
Adenoviridae ; genetics ; Animals ; Antigens, CD19 ; blood ; CD3 Complex ; blood ; Disease Models, Animal ; Female ; Fetal Blood ; transplantation ; Immunoglobulin G ; blood ; Interferon-gamma ; metabolism ; Leukocyte Common Antigens ; blood ; Male ; Mice ; Mice, SCID ; Oncogene Proteins, Fusion ; genetics ; immunology ; Oncogene Proteins, Viral ; genetics ; immunology ; Papillomaviridae ; genetics ; Recombination, Genetic ; T-Lymphocytes ; cytology ; Viral Vaccines ; immunology
8.Association of hepatitis B virus infection and cirrhosis with liver metastasis in colorectal cancer.
Hong-gang QIAN ; Ji ZHANG ; Jia-hua LENG ; Guo-quan ZHOU ; Jian-hui WU ; Xiu-yun TIAN ; Yong YANG ; Chun-yi HAO
Chinese Journal of Gastrointestinal Surgery 2010;13(3):202-204
OBJECTIVETo explore the relationship of hepatitis B virus (HBV) infection and cirrhosis with liver metastasis in colorectal cancer.
METHODSClinical date of 1176 colorectal cancer patients undergone surgical treatment in the Peking University School of Oncology between January 1999 and August 2004 were analyzed retrospectively to investigate the impact of HBV infection and cirrhosis on the occurrence of liver metastasis and prognosis of patients.
RESULTSThe incidence of liver metastasis was 8.8% (10/114) in the HBV infection group and 23.9% (254/1062) in the non-infection group (P<0.01). The 5-year survival rates of these two groups were 54.2% and 60.7% ( P>0.05). The incidence of liver metastasis was 3.8% (1/26) in the cirrhotic group and 22.9% (263/1150) in the non-cirrhotic group (P<0.05). The 5-year survival rates of these two groups were 60.9% and 59.9% ( P>0.05).
CONCLUSIONBoth hepatitis B virus infection and cirrhosis are associated with less liver metastasis in colorectal cancer, but have no impact on the survival.
Adult ; Aged ; Aged, 80 and over ; Colorectal Neoplasms ; pathology ; virology ; Female ; Hepatitis B ; complications ; Hepatitis B virus ; Humans ; Liver Cirrhosis ; complications ; Liver Neoplasms ; secondary ; virology ; Male ; Middle Aged ; Neoplasm Metastasis ; Retrospective Studies ; Survival Rate ; Young Adult
9.Serum adiponectin and free fatty acid profile in essential hypertensive patients with or without metabolic syndrome.
Jia-Qiang LI ; Miao-Ying LI ; Ying-Xiu DAI ; Yu-Min LIU ; Ji ZHENG ; Wen-Bin LIU
Chinese Journal of Cardiology 2008;36(3):232-235
OBJECTIVETo observe serum adiponectin, free fatty acid (FFA) profile and other glucose and lipid metabolic parameters in essential hypertensive patients with metabolic syndrome (HPMS) or without metabolic syndrome (HP).
METHODSThe serum adiponectin was measured with the radioimmunoassay, FFA profile measured with the gas chromatography and mass spectrometer in 72 HPMS, 56 HP and 43 normal control subjects.
RESULTSSerum adiponectin were significantly lower in HPMS group than those in the HP and control group (P < 0.05 or P < 0.01). Serum total free fatty acid (TFA), unsaturated fatty acid (UFA, linoleic acid, oleic acid, arachidonic acid, docosahexaenoic acid, eicosatrienoic acid), polyunsaturated fatty acid (PUFA) and n6PUFA were significantly higher in HPMS group than those in the HP and control groups (P < 0.05 or P < 0.01). Adiponectin was negatively correlated with body mass index, waist circumferences, waist hip ratio, triglycerides (r = -0.222, -0.235, -0.179, -0.194, P < 0.01 or P < 0.05, respectively) and positively correlated with high-density lipoprotein cholesterol (r = 0.336, P < 0.01); TFA and PUFA were positively correlated with body mass index, waist circumferences, fasting blood glucose, mean arterial pressure (r = 0.241 and 0.280, 0.198 and 0.188, 0.226 and 0.298, 0.274 and 0.334, P < 0.01 or P < 0.05, respectively) in all subjects.
CONCLUSIONOur data suggest that changes in serum adiponectin, FFA and n6PUFA might promote the development of metabolic syndrome in essential hypertensive patients.
Adiponectin ; blood ; Aged ; Fatty Acids, Nonesterified ; blood ; Female ; Humans ; Hypertension ; blood ; complications ; epidemiology ; Male ; Metabolic Syndrome ; blood ; complications ; epidemiology ; Middle Aged
10.Littoral cell angioma of the spleen: report of three cases and a review of the literature.
Xiao-Yan LIN ; Jia-Mei LI ; Qiang-Xiu WANG ; Ji-Zhen FENG ; Miao-Qing ZHAO ; Wei-Xia ZHONG ; Jian-Feng LI
Chinese Medical Journal 2011;124(20):3423-3426
Littoral cell angioma is a recently described rare vascular tumor of the spleen. The clinical course of this benign tumor is asymptomatic in most patients. Herein, we described three patients with littoral cell angioma detected during physical examination. A brief discussion and review of a handful of cases of splenic littoral cell angioma, which have been previously reported in the English language literature, are performed in this paper.
Female
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Hemangioma
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blood
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diagnosis
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pathology
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Humans
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Male
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Middle Aged
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Splenic Neoplasms
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blood
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diagnosis
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pathology