1. Inhibitory effect of combined transfection of antisense oligonucleotides of VEGF-C and COX-2 on the expression of VEGF-C in breast carcinoma cell line
Tumor 2008;28(2):117-120
Objective: To investigate the effect of combined transfection of antisense oligonucleotides (ASODNs) of vascular endothelial growth factor-C (VEGF-C) and cyclooxygenase-2 (COX-2) by cationic liposome (LIP) on the expression of VEGF-C in breast carcinoma MDA-MB-231 cells. Methods: Cytotoxicity of ASODNs on MDA-MB-231 cells was measured by MTT assay and the optimal concentration for transfection was determined. The transcription of VEGF-C in breast carcinoma cells was detected by RT-PCR. The protein expression of VEGF-C was detected by immunohistochemical staining and confocal laser scanning microscopy in MDA-MB-231 cells after co-transfection of VEGF-C and COX-2 ASODN. Results: No cytotoxic effect was found when ASODNs/LIP was transfected at 0.2 mmol/L + 2.5 mL/L. The inhibitory ratio on VEGF-C mRNA transcription was 70.37% in the combined transfection group, which was significantly higher than VEGF-C transfection group and COX-2 transfection group (48.15% and 42.21%, P < 0.01). The protein expression of VEGF-C was significantly lower in the combined transfection group than that in only VEGF-C or COX-2 transfection group (P < 0.01). Conclusion: Combined transfection of VEGF-C and COX-2 ASODN markedly decreases the expressions of VEGF-C at mRNA and protein levels in MDA-MB-231 cells. COX-2 maybe involved in the induction of VEGF-C expression in breast cancer.
4.A case of congenital leukemia.
Xiu-li YUAN ; Chang-gang LI ; Yun-sheng CHEN
Chinese Journal of Pediatrics 2005;43(7):498-498
Fatal Outcome
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Humans
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Infant, Newborn
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Leukemia
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blood
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congenital
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diagnosis
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Leukocyte Count
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Male
5.Relationship between artesunate influence on the process of TGF-beta1 induced alveolar epithelial cells transform into mesenchymal cells and on idiopathic pulmonary fibrosis.
Chang-Ming WANG ; Juan CHEN ; Ming JIANG ; Xiu-Ping XUAN ; Hong-Xiu LI
Acta Pharmaceutica Sinica 2014;49(1):142-147
This study is to investigate the effect of artesunate on transforming growth factor-beta1 (TGF-beta1) induced epithelial-mesenchymal transition (EMT) and its possible mechanism. After the in vitro cultured RLE-6TN cells were treated with TGF-beta1 then artesunate intervened on it, after 24 h, expression of the markers of mesenchymal cell was assayed using Western blotting and real-time PCR analysis. Western blotting was also used to detect the effect of TGF-beta1 on the Smad3 and Smad7 expressions of RLE-6TN cells. Morphological alterations were examined by phase-contrast microscope, and ultrastructure changes by electron microscope. Incubation of RLE-6TN cells with TGF-beta1 resulted in the up-regulation of the expression of the mesenchymal cell markers, after artesunate intervened on it, resulted in the down-regulation of the expression. Meanwhile, incubation with artesunate intervened on RLE-6TN cells could lead to the apparent down-regulation of the expression of Smad3 and up-regulation of Samd7 and the transition of RLE-6TN cells to mesenchymal-like by TGF-beta1 induction, after artesunate intervened on it, RLE-6TN cells to epithelial-like. TGF-beta1 induced epithelial-mesenchymal transition process; artesunate can inhibit TGF-beta1-induced epithelial-mesenchymal transition process, the possible mechanism is up-regulation of the expression of Smad7 and down-regulation of the expression of Smad3, meanwhile inhibits phosphorylation of Smad3.
Actins
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genetics
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metabolism
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Animals
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Artemisia
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chemistry
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Artemisinins
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isolation & purification
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pharmacology
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Cell Line
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Cell Proliferation
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drug effects
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Epithelial Cells
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cytology
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metabolism
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Epithelial-Mesenchymal Transition
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drug effects
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Idiopathic Pulmonary Fibrosis
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pathology
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Plants, Medicinal
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chemistry
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Pulmonary Alveoli
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cytology
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RNA, Messenger
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metabolism
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Rats
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Smad3 Protein
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genetics
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metabolism
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Smad7 Protein
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genetics
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metabolism
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Transforming Growth Factor beta1
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pharmacology
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Vimentin
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genetics
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metabolism
6.Changes of Brain Natriuretic Peptide and Atrial Natriuretic Peptide in Serum of Children with Congestive Heart Failure
hong, CHANG ; xiu-zhen, HAN ; wen-li, WANG
Journal of Applied Clinical Pediatrics 2003;0(10):-
Objective To study the serum level of brain natriuretic peptide (BNP) and atrial natriuretic peptide (ANP) in patients with congestive heart failure (CHF) and assess the value of BNP. Methods The serum levels of BNP and ANP were determined by enzyme- linked immnoabsorbent assay.Cardial mdex(CI)and left ventricular election fraction(LVEF)in heart failure stage and period recouery of children with heart failare were determined by Doppler ultrasonography cardiogram. Results The BNP serum levels began to increase before heart failure (P
10.Assessment of cerebral blood flow with ~(99m)Tc-ECD perfusion imaging for children with school phobia
xiu-li, GAO ; yong-li, YU ; chang-qing, JIN ; yun, QIAN ; ya-song, DU
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(09):-
ObjectiveTo explore the clinical and epidemiological value of semi-quantitative regional cerebral blood flow(rCBF)imaging in children with school phobia. Methods A total of 20 cases diagnosed with school phobia were examined with rCBF.Twelve were males and the other 8 were females.The mean age was(14.2?2.1)years(11-18 years).Semi-quantitative analysis methods were used to investigate the correlations among gender,age and rCBF. ResultsThere were significant differences in the rCBF of right fronto-parietal lobe,right occipital lobe,caput and putamen,left thalamus and hippocampus,and temporo-occipital lobe between males and females(P0.05).Only the PI of left temporo-parietal lobe of those ≥ 15 years old was significantly different from that of those