1.The effects of curcumin on PTEN/PI3K/Akt pathway in Ec109 cells.
Xiu-juan LI ; Qiang LUO ; Li SUN ; Hua LIT ; Chun-ting JIN ; Jie FAN ; Yu-zhen LI
Chinese Journal of Applied Physiology 2015;31(5):465-468
OBJECTIVETo investigate the inhibition effect of curcumin on the proliferation of the human esophageal carcinoma cell line Ec109 and its impact on PEN/PI3K/Akt signaling pathway.
METHODSEsophageal carcinoma Ec109 cells were cultured in vitro conventionally and were treated with curcumin at different concentrations. The cell proliferation level was examined by MIT colorimetry, the ultrastructure of curcumin-treated Ec109 cells were detected with transmission electron microscope (TEM) and cell apoptosis was observed by FCM with AnnexinV-FITC/PI double staining. The protein levels of PTEN, Akt, GSK3P and Caspase 3 of curcumin-treated Ec109 cells were detected by Western blot.
RESULTSMTT test showed that curcumin could inhibit the proliferation of Ec109 cells in a time and concentration-dependent manner. TEM examination indicated that curcumin could induce Ec109 cell apoptosis. FCM detection showed that Ec109 cell apoptotic rate increased significantly with the increase of drug concentration. On the other hand, curcumin could promote the expression of PTEN, GSK3beta and Caspase 3 yet reduce the expression of Akt.
CONCLUSIONCurcumin could obviously up-regulate the expression of PTEN, GSK3beta and Caspase 3, surpress PI3K/Akt signaling pathway and hence inhibit the proliferation of Ec109 cells.
Apoptosis ; drug effects ; Caspase 3 ; metabolism ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Curcumin ; pharmacology ; Glycogen Synthase Kinase 3 ; metabolism ; Glycogen Synthase Kinase 3 beta ; Humans ; Oncogene Protein v-akt ; metabolism ; PTEN Phosphohydrolase ; metabolism ; Phosphatidylinositol 3-Kinases ; metabolism ; Signal Transduction ; drug effects
2.Chemical Constituents in Volatile Oil from Fruits of Alpinia oxyphylla Miq.
Xiu-Zhen LUO ; Jing-Guang YU ; Li-Zhen XU ; Shi-Lin YANG ; Jing-Dong FENG ; Shu-Ling OU
China Journal of Chinese Materia Medica 2001;26(4):262-264
Objective:To study the chemical constituents in the volatile oil from fruits of Alpinia oxy-phylla.Method:Using GC-MS to identify the constituents.Result and Conclusion:Sixty-four compounds were identified on the basis of GC-MS, the main ones being p-cymene, valencene, linalool, myrtenal, α-pinene, β-pinene, furopelargone A and terpinen-4-ol.Three sesquiterpenes valencene, nootkanone and nootkanol have been isolated from the CHCl3 extract as check,of these 64 identiified compounds linalyl oxide, valencene, bakkenolide A, furopelargone A and 3-hydroxycalamenene are reported for the first time.
3.Two flavone glycosides from Chinese traditional medicine Amomum villosum.
Lan SUN ; Jing-guang YU ; Li-dong ZHOU ; Xiu-zhen LUO ; Wei DIN ; Shi-lin YANG
China Journal of Chinese Materia Medica 2002;27(1):36-38
OBJECTIVETo study the water soluble constituents from Amomum villosum.
METHODThe constituents were separated and purified with chromatographic methods, identified by NMR, MS, UV and IR.
RESULTTwo quercetin glycosides: quercitrin (quercetin-3-O-alpha-L-rhamnoside I) and isoquercitrin (quercetin-3-O-beta-D-glucoside II) were isolated and identified.
CONCLUSIONI and II were isolated for the first time from A. villosum.
Amomum ; chemistry ; Fruit ; chemistry ; Plants, Medicinal ; chemistry ; Quercetin ; analogs & derivatives ; chemistry ; isolation & purification
4.Chemical constituents from the seeds of Annona squamosa.
Jing-guang YU ; Xiu-zhen LUO ; Lan SUN ; De-yu LI ; Wen-hua HUANG ; Chun-yu LIU
Acta Pharmaceutica Sinica 2005;40(2):153-158
AIMTo study the antitumor active constituents of the seeds from Annona squamosa L. (Annonaceae).
METHODSVarious chromatographic techniques were used to isolate and purify the constituents. Their physico-chemical properties and spectral data were determined to elucidate the structures.
RESULTSEleven compounds were isolated and identified as annonaceous acetogenins: squamocenin (1), annotemoyin-2 (2), reticulatain-2 (3), squamocin-I (4), squamocin-B (5), squamocin (6), motrilin (7), squamostatin-D (8), squamostatin-E (9), cherimolin-1 (10), cherimolin-2 (11) from the ethyl alcohol extract of A. squamosa L.
CONCLUSIONSquamocenin (1) is a new acetogenin. Annotemoyin-2 (2) and reticulatain-2 (3) were isolated from this plant for the first time.
Annona ; chemistry ; Furans ; chemistry ; isolation & purification ; Lactones ; chemistry ; isolation & purification ; Molecular Conformation ; Molecular Structure ; Plants, Medicinal ; chemistry ; Seeds ; chemistry
5.Study of TRAIL receptors expression on the mononuclear cells from multiple myeloma patients and KM3 cells.
Juan LI ; Jun-He LI ; Shao-Kai LUO ; Yin ZHAO ; Guo-Cai ZHANG ; Dong ZHENG ; Xiu-Zhen TONG ; Ai-Hua PENG
Chinese Journal of Hematology 2005;26(4):214-217
OBJECTIVETo study the differential expression of four TRAIL receptors on bone marrow mononuclear cells (BMMNC) from multiple myeloma (MM) patients and myeloma cell line KM3 cells, to compare their altered expressions after chemotherapy and to explore the mechanisms by which TRAIL selectively kills tumor cells.
METHODSSemi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and flow cytometry were used to investigate the expression of four TRAIL receptors on BMMNCs in 23 MM patients, KM3 cells and 15 controls, and the changes of their expression pattern after chemotherapy and after incubation of KM3 cells with sub-clinical concentration of doxorubicin.
RESULTSDR4 and DR5 were highly expressed on KM3 cells with no expression of DcR1 and DcR2. Expressions of DR4 and DR5 on BMMNCs from MM patients were higher and expression of DcR1 and DcR2 were lower than that of controls (P < 0.05). The expression of DR5 on MM and KM3 cells was up-regulated after chemotherapy and exposure to doxorubicin (P < 0. 05).
CONCLUSIONSThe expressions of four TRAIL receptors on myeloma cells and normal controls were different, which might account for the selective killing effect of TRAIL on MM cells. Up-regulated DR5 on KM3 cells after incubating with doxorubicin and after chemotherapy suggests the cytotoxic agents might enhance the apoptosis of MM cells.
Antineoplastic Agents ; pharmacology ; Cell Line, Tumor ; Cells, Cultured ; Doxorubicin ; pharmacology ; Female ; Flow Cytometry ; Gene Expression ; drug effects ; Humans ; Leukocytes, Mononuclear ; cytology ; drug effects ; metabolism ; Male ; Middle Aged ; Multiple Myeloma ; drug therapy ; genetics ; pathology ; Receptors, TNF-Related Apoptosis-Inducing Ligand ; biosynthesis ; genetics ; Reverse Transcriptase Polymerase Chain Reaction
6.Clonal expansion of TCR Vbeta subfamily T cells induced by bcr3-abl2 peptide.
Yu-ping ZHANG ; Yang-qiu LI ; Li-jian YANG ; Shao-hua CHEN ; Xue-li ZHANG ; Zhen WANG ; Xiu-li WU ; Geng-xin LUO
Chinese Journal of Hematology 2004;25(2):95-99
OBJECTIVETo investigate the clonal expansion of T cell receptor (TCR) Vbeta subfamily T cells from cord blood induced by bcr3-abl2 peptide in vitro.
METHODST cells from 3 units of cord blood were amplified by anti-CD(3) monoclonal antibody (McAb) and IL-2 with or without synthetic b3a2 peptide. T cell specific cytotoxicity was analyzed by lactate dehydrogenase (LDH) assay, TCR Vbeta subfamilies by using reverse transcriptase-polymerase chain reaction (RT-PCR) and genescan technique.
RESULTSbcr3-abl2 peptide specific cytotoxicity T cells were successfully induced from the 3 units of cord blood by synthetic b3a2 peptide. Compared with that in CD(3) McAb induced cells, distribution pattern of TCR Vbeta repertoire was different in T cells induced with b3a2 peptide. Oligoclonal and oligoclonal tendency TCR Vbeta subfamily T cells could be identified in cord blood T cells induced by b3a2 peptide in 1 or 2 weeks, whereas those induced by anti-CD(3) McAb and IL-2 were mostly polyclonal.
CONCLUSIONThe cytotoxicity T cells with anti-CML specificity could be induced by b3a2 peptide. The specific anti-CML cytotoxicity may be derived from the clonal expansion TCR Vbeta subfamily T cells.
Antibodies, Monoclonal ; immunology ; CD3 Complex ; immunology ; Fusion Proteins, bcr-abl ; pharmacology ; Genes, T-Cell Receptor beta ; Humans ; Interleukin-2 ; pharmacology ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive ; immunology ; T-Lymphocytes, Cytotoxic ; immunology
7.Emotional problems and parenting locus of control in children with anxiety disorders.
Ling SHEN ; Xue-Rong LUO ; Zhen WEI ; Bing-Qing GUAN ; Xiu-Hong YUAN ; Zhi-Jun NING ; Jun DING ; Wei YANG
Chinese Journal of Contemporary Pediatrics 2011;13(12):970-972
OBJECTIVETo carry out a preliminary study on the emotional problems and parenting locus of control among children with anxiety disorders.
METHODSA total of 110 children with simple anxiety disorders (AD group) and 113 normal children (control group) from September to December 2005 were enrolled. Children were asked to complete the Depression Self-Rating Scale for Children (DSRSC), the Screen for Child Anxiety Related Emotional Disorders (SCARED), and the Parenting Locus of Control Scale (PLOC).A total of 197 valid scales were returned.
RESULTSThe scores of somatic, generalized anxiety, separation anxiety, social phobia anxiety, school phobia anxiety, total anxiety, and total depression were all higher in the AD group than in the control group (P<0.01). The score of "education effects" for parents was significantly higher in the AD group than that in the control group (P<0.01).
CONCLUSIONSChildren with anxiety disorders tend to have more emotional problems and poorer parental education effects.
Adolescent ; Affective Symptoms ; etiology ; Anxiety Disorders ; psychology ; Child ; Female ; Humans ; Internal-External Control ; Male ; Parenting
8.Prevalence of psychiatric disorders in primary and middle school students in Hunan Province.
Bing-Qing GUAN ; Xue-Rong LUO ; Yun-Long DENG ; Zhen WEI ; Hai-Sen YE ; Xiu-Hong YUAN ; Zhi-Jun NING ; Wei YANG ; Jun DING
Chinese Journal of Contemporary Pediatrics 2010;12(2):123-127
OBJECTIVETo investigate the prevalence of psychiatric disorders in a representative sample of primary and middle school students in Hunan Province.
METHODSA total of 9 495 children aged 5-17 years from Hunan urban and rural schools were enrolled by a cluster sampling and a two-phase design. The students' psychiatric status was assessed using the Investigation Screening Inventory for Child Mental Disorder and a semi-structured interview designed based on the DSM-IV criteria.
RESULTSThe overall prevalence of psychiatric disorders was 16.22%. Attention-deficit and disruptive behavior disorders were the commonest in the diagnostic categories of psychiatric disorders (10.69%). Regarding specific disorders, the most prevalent was attention-deficit/hyperactivity disorder (5.95%). Psychiatric disorders were more prevalent in boys than in girls (20.49% vs 11.16%; p<0.01). The prevalence of attention-deficit and disruptive behavior disorders in boys was higher than in girls (14.76% vs 5.87%; p<0.01). The prevalence of psychiatric disorders in middle school students (12-17 years) was significantly higher than in primary students (5-11 years) (18.38% vs 14.64%; p<0.01). There were no significant differences in the prevalence of psychiatric disorders between urban and rural students.
CONCLUSIONSPsychiatric disorders are common among primary and middle school students in Hunan Province. The prevalence of this disorder in boys is higher than in girls. The middle school students have higher prevalence than primary students.
Adolescent ; Age Factors ; Child ; Child, Preschool ; China ; epidemiology ; Female ; Humans ; Male ; Mental Disorders ; epidemiology ; Prevalence ; Sex Factors
9.A case-control study on family environment related factors in attention deficit hyperactivity disorder with anxiety disorder
Yue-Bing ZHANG ; Xue-Rong LUO ; Xia LIU ; Zhen WEI ; Bing-Qing GUAN ; Xiu-Hong YUAN ; Hai-Sen YE ; Zhi-Yun NING ; Wei YANG ; Jun DING ; Yun-Long DENG
Chinese Journal of Epidemiology 2009;30(2):119-122
Objective To study the family rearing pattern of attention deficit hyperactivity disorder(ADHD)with or without anxiety disorder and to explore its risk factors.Methods 9495 children and their parents were sampled at random in Hunan province,using two-stage investigation.Those who were diagnosed ADHD and the normal control filled out Egna Minnen av Barndoms Uppfostran and family adaptability and cohesion scale bv themselves.Results The comparison of factors as:actual family cohesion,parents' punishments,reiection,mother's excessive protection,intervention and father's excessive protection were significantly difierent between ADHD with or without anxiety disorder and normal children(P<0.05).The comparison of parents' punishments,reiection,excessive protection and intervention were obviously different between ADHD with anxiety disorder and simple ADHD(P<0.05).Mother's reiection was the influencing factor of simple ADHD,with OR as 1.122.Ideal family cohesion,mother's rejection and father's punishments were the influencing factors of ADHD with anxiety disorder,with OR as 0.966.1.215 and 1.089 respectively.Conclusion There were some problems in the parental rearing pattern of ADHD with or without anxiety disorder.Mother's rejection,father's punishments and ideal family cohesion were suggested to be correlated with ADHD and anxiety disorder.
10.Investigation of work pressure status in community nursing personnel
Ju-Zhen LUO ; Xiu-Xiang CHEN ; Yan-Hong WANG ; Yan SU ; Yan-Hua WANG ; Li-Ping ZHU ; Jin-Feng TAO
Chinese Journal of Modern Nursing 2010;16(34):4107-4108
Objective To understand the work pressure status in community nursing personnel in Longgang district of Shenzhen, to look for the ways to reduce their work pressure. Methods Through questionnaire, with work pressure source quantity table of China nurses, 200 community nurses in Longgang district of Shenzhen were randomly investigated, the effective questionnaire of 198 community nurees in Longgang district of Shenzhen were collected for statistical analysis. Results The social position of nursing jobs is low, the quantity of nurses on duty is not enough, the work load of nurses is too heavy, non-nursing work is too much; the opportunity of further education is rare. Conclusions The structure of human resources in community nursing personnel should be properly adjusted; community nursing management model should be perfected.