Antifungal Agents ; therapeutic use ; Aspergillus fumigatus ; pathogenicity ; Brain Abscess ; diagnostic imaging ; drug therapy ; microbiology ; Humans ; Infant ; Male ; Neuroaspergillosis ; complications ; pathology ; Occipital Lobe ; pathology ; Pulmonary Aspergillosis ; diagnostic imaging ; drug therapy ; microbiology ; Tomography, X-Ray Computed ; Treatment Outcome

Adolescent ; Biopsy ; Cough ; etiology ; Diagnosis, Differential ; Female ; Fever ; etiology ; Humans ; Lung ; diagnostic imaging ; pathology ; Sarcoidosis, Pulmonary ; diagnosis ; diagnostic imaging ; pathology ; Tomography, X-Ray Computed

A separation technology of catalpol from Rehmannia with macroporpus adsorbent resins was investigated. The content of catalpol in the extract was determined by high performance liquid chromatography (HPLC). Nine different kinds of macroporous adsorbent resins were studied on the static capacity of adsorption and desorption, and D101 resin was best for the separation of the extraction solution of Rehmannia. The results showed that D101 resin had the highest static adsorption capacity of 69.2mg/g dry resin and its isotherm curve can be well described by Langmuir and Freudlich equation. The 5% ethanol elution on removal of the solvent under reduced pressure provided a brown powder, which was subjected to an open column chromatography on silica gel eluted with a CHCl3–MeOH gradient. The fraction eluted with CHCl3-MeOH (8∶2) was identified as catalpol and the purity of the compound was more than 90% purity by HPLC analysis. The yield of this separation technology was 6%.

Objective To investigate the com and pepper fluorine in Zhenxiong county of Yunnan province, as well as the change of com and pepper fluorine after baked by coal, clay-mixed with cual for a relatively long-term, in order to provide a scientific basis for reducing fluorine intake. Methods The endemic areas of Yile, Wufeng's Songlinwan, Tangfang and Wufeng's Wugu in Zhenxiong county, and a non-endemic area Xiaguan in Dali city were selected as study sites. Ten samples of fresh corn and pepper were collected in each region, and fluorine was determined using acid leaching/potentiometry freshly and after baking or drying for 10 days or 4 months, respectively. Results The fluorine content of local fresh corn in Xiaguan of Dali city and Yile,Wufeng's Songlinwan, Tangfang, Wufeng's Wugu in Zhenxiong county were (1.31 ± 0.13),(1.65 ± 0.64),(1.92 ±0.37), (2.32 ± 0.49), (1.98 ± 0.66)mg/kg, respectively, and there were statistically significant differences across the regions(H = 27.871, P ＜ 0.05). The fluorine content of corn samples after baking or drying for 4 months were ( 1.82 ± 0.17), (26.43 ± 12.03), (39.27 ± 8.09), ( 14.27 ± 4.37), ( 14.33 ± 1.73)mg/kg, respectively, which were significantly higher than that of the fresh com in the corresponding region(all P ＜ 0.05 ), and there were statistically significant differences across the regions(H = 42.512, P＜ 0.05). The fluorine content of the local fresh chili were (3.34 ± 1.08), (3.44 ± 0.55), (3.47 ± 0.74), (3.46 ± 0.93)mg/kg, respectively, in the 4 observed places in Xiaguan of Dali city and Yile, Wufeng's Songlinwan, Tangfang in Zhenxiong county, and there were no statistically significant differences across the regions (F = 0.052, P ＞ 0.05 ). The fluorine content of pepper samples after baking or drying for 4 months were (7.01 ± 1.64), (226.07 ± 83.69), (179.36 ± 148.37), (54.51 ± 34.67)mg/kg,respectively, which were significantly higher than that of the fresh pepper in the corresponding region(all P ＜ 0.05 ),and there were statistically significant differences across the regions(H = 28.822, P ＜ 0.05). Conclusion Corn and chili fluorine is significantly increased after baked with coal and clay-mixed with coal by farmers in Zhenxiong county, a coal- burning borne endemic fluorosis area of Yunnan province.

Endophytic bacteria which was producing indoleacetic acid was screened from Panax ginseng by using the Salkowski method. The active strain was also tested for its ability of nitrogen fixation by using the Ashby agar plates, the PKV plates and quantitative analysis of Mo-Sb-Ascrobiology acid colorimetry was used to measure its ability of phosphate solubilization, for its ability of potassium solubilization the silicate medium and flame spectrophotometry was used, for its ability of producing siderophores the method detecting CAS was used, for its ability of producing ACC deaminase the Alpha ketone butyric acid method was applied. And the effect on promoting growth of seed by active strain was tested. The results showed that the indoleacetic acid producing strain of JJ5-2 was obtained from 118 endophytes, which the content of indoleacetic acid was 10.2 mg x L(-1). The JJ5-2 strain also had characteristics of phosphate and potassium solubilization, nitrogen fixation, producing siderophores traits, and the promoting germination of ginseng seeds. The JJ5-2 strain was identified as Bacillus thuringiensis by analyzing morphology, physiological and biochemical properties and 16S rRNA gene sequences.
Bacteria ; isolation & purification ; metabolism ; Endophytes ; isolation & purification ; metabolism ; Indoleacetic Acids ; metabolism ; Panax ; microbiology

Objective To explore the health education methods in malnutritional patients on maintenance hemodialysis using the transtheoretical model and stages of change (TTM).And also to help patients to set up scientific behavioral patterns to improve their self- management ability and reduce the incidence of complications.Methods 72 maintenance hemodialysis patients were included in this study.Taking TTM as intervention,we compared the nutritional status and the modified quantitative subjective global assessment (MQSGA) at 4 different time points:before intervention,1month,3 months and 6 months after intervention respectively,and statistically analyzed the data.Results Compared with before intervention,HGB level elevated significantly 1month after intervention ( q =2.08,P ＜ 0.05 ),while ALB was not changed significantly(q =1.22,P ＞ 0.05 ),HCRP dropped significantly ( q =3.06,P ＜ 0.01) and Kt/V increased significantly(q =4.12,P ＜0.01).HGB and Kt/V level increased significantly 3 months after intervention(q =8.20,6.13;P＜0.01),ALB level increased(q =2.81,P ＜0.05),while HCRP dropped significantly(q =8.85,P ＜0.01).HGB,ALB and Kt/V levels all increased significantly 6 months after intervention compared with 3 months after intervention (q =10.89,7.06,9.68,respectively; All P ＜ 0.01),and HCRP dropped significantly(q =13.26,P ＜0.01).The MQSGA improved significantly at both 3 months( x2 =15.84,P ＜0.01)and 6 months( x2 =86.26,P ＜0.01)after intervention compared with before intervention.Conclusions By using the TTM,we can change the life style of the hemodialysis patients,and improve their nutritional status and life quality.

Objective To explore the positive effect of nephrotic syndrome in patients with recurrent after carrying out health education intervention.Methods 60 cases were divided into the experimental group and control group (30 cases in each group).In the control group,general health education mode was used; and the experimental group patients according to the reason of relapsing again,were implemented the targeted health education; compare two sets of patients' serum albumin (ALB),24 h urine protein quantitative level and satisfaction,and six months after discharge from the hospital again.Results Before education the ALB and 24 h urine protein quantitative level of the two sets of patients was statistically insignificant ( P ＞ 0.05 ).After the experimental education for patients with ALB ( 30.57 ± 4.01 ) higher than those in the control group (25.23 ±4.71 ) ; The 24 h urine protein for quantitative (4.04 ± 0.94 ) was significantly lower than the control group (5.42 ±1.41 ),two groups of comparisons difference were statistically significant (t=4.73-4.47,respectively ;P ＜ 0.01 ).The satisfaction of patients was 100.0％ in experiment group and 90.0％ in the control group,and the difference was significant ( x2=7.29,P ＜ 0.05 ) ; six months after discharge of experimental group' s hospital admission rates significantly lower than those of the control group again ( 13.3％ vs 30.0％ ;x 2=5.46,P ＜ 0.05 ).Conclusions Implementation of health education to the nephrotic syndrome in patients with recurrent is beneficial to access relevant health knowledge,but also reduce the external factors leading to disease recurrence and increase the care satisfaction of hospitalized patients.

OBJECTIVESTo establish a primary biliary cirrhosis (PBC) model by AMAM2 autoantigen injection into C57BL/6 mice.

METHODSMice of the model group were immunized intraperitonealy with 200 microl of purified recombinant AMAM2 autoantigen in complete Freund's adjuvant (CFA). Mice immunized with bovine serum albumin and CFA in the same way were used as negative controls. Sixty-six weeks later, mice were sacrificed and their sera were collected. Sera samples were assayed for AMAM2 autoantibody, alkaline phosphatase (ALP), ALT and total bilirubin (TBil). Their liver, stomach, muscle and kidney tissues were sectioned and stained using HE to observe the pathological changes.

RESULTSAntibodies to AMAM2 autoantigen were readily induced in the model group. The mice in the model group had no significant changes in the level of serum ALT and TBil but had an obvious increase of ALP (P<0.05). The stomach, muscle and kidney tissues showed no evident damage while the livers had obvious pathological changes, including bile duct degeneration or proliferation, and mononuclear cell infiltration.

CONCLUSIONThe AMAM2 autoantigen-induced PBC animal model was successfully established in C57BL/6 mice in our experiment and its characteristic biochemical and pathology are quite similar to that in the early stage of human PBC. This model may provide a useful experimental approach for further study of the pathogenesis and clinical treatment of human PBC.

Animals ; Autoantigens ; immunology ; Disease Models, Animal ; Liver Cirrhosis, Biliary ; etiology ; Mice ; Mice, Inbred C57BL ; Mitochondria ; immunology

To evaluate the significance of immunologic classification for typing of acute leukemia (AL). 68 cases of AL were classified by morphologic and immunologic typings. The results showed that the consistency rate was 94.1% between morphology and immunology, and 4 morphologic misdiagnosed cases were corrected by immunology; CD13 and CD33 were special myeloid lineage-associated antigens; AML-M(3) was often CD34 low-expressed and HLA-DR-negative; CD14 was often expressed in AML-M(4) and M(5); lymphoid lineage-associated antigens (CD7) were easily found in ANLL, and myeloid lineage-associated antigens were also found in ALL. In conclusion, immunologic classification can improve the accuracy in acute leukemia diagnosis. The diagnosis of some special AL, such as acute unidentified leukemia (AUL), AML-M(0) and so on, must rely on immunologic classification.
Adolescent ; Adult ; Aged ; Antigens, CD ; biosynthesis ; Antigens, CD34 ; biosynthesis ; Antigens, CD7 ; biosynthesis ; Antigens, Differentiation, Myelomonocytic ; biosynthesis ; CD13 Antigens ; biosynthesis ; Female ; Humans ; Immunophenotyping ; Leukemia, Myeloid, Acute ; classification ; immunology ; Lipopolysaccharide Receptors ; biosynthesis ; Male ; Middle Aged ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; classification ; immunology ; Sialic Acid Binding Ig-like Lectin 3

OBJECTIVEDescribe the mental health services provided at different types of general hospitals in Beijing.

METHODSDistribute the "Hospital Mental Health Services Survey" to all 508 hospitals in the 18 Beijing districts; the survey covers information for 2001 on the type of mental health services provided, the characteristics of the service providers, the number and diagnoses of first-visit mental health patients, and the prescription of psychotropic medications.

RESULTS73% (373/508) of the hospitals completed the survey, including 75% (325/436) of the general hospitals. Among the 325 general hospitals, 171 (53%) provided one or more types of mental health services; they had a total of 420 staff members who provide these services, but their educational level was relatively low and mental health training limited. 61 general hospitals had mental health outpatient departments that treated 50% (47,151/93,891) of all first-visit mental health patients seen in all hospitals (including psychiatric hospitals) in the municipality; 90% (42,578/47,151) of these first-visit patients in general hospitals were seen at 18 level-3 hospitals. Among the 44,441 first-visit patients for whom diagnostic information was available, the main diagnoses were neurosis (43%) and depression (30%). 103 of the participating general hospitals prescribed 73% of antidepressant medications prescribed at all hospitals in the municipality and 97% of all fluoxetine (Prozac and others).

CONCLUSIONSGeneral hospitals-particularly level-1 and level-2 general hospitals-need to put more emphasis on mental health services, increase training of staff who provide the services and open relevant departments. Monitoring the development of mental health services in general hospitals and increasing the quality of their mental health services will require periodic assessment of the types and range of mental health services provided and of the diagnostic accuracy and treatment standards of the staff providing these services.

Adult ; Antidepressive Agents ; therapeutic use ; China ; Drug Utilization ; statistics & numerical data ; Female ; Hospitals, General ; statistics & numerical data ; Humans ; Male ; Mental Health Services ; statistics & numerical data ; Middle Aged ; Surveys and Questionnaires