Objective To analyse the causes of perinatal death and explore the preventive measures to reduce the perinatal mortality.Methods The cases with perinatal death in this hospital from January 2005 to December 2006 were reviewed to analyse the causes of death by categorization and sum-up.Results There were 166 cases with perinatal death and the mortality rate was 27.08‰,including 126 cases with fatal death,which accounted for 75.90%.In the analysis of dead causes,the first one was birth defects,which suffered 69 cases,41.57% of all,and mostly were with fetus edema syndrome.The cord factors had been elevated to the second cause,which suffered 51 cases,30.72% of all.Conclusion Improving the consciousness of gestational monitoring and self-care,strengthening the prenatal diagnosis and genetic counseling,controlling the perinatal birth defects,monitoring mother and fetus by poly-parameter and stopping the pregnancy in time can reduce perinatal death effectively.

Objective To explore the coordination of a day in the operating room on the surgery arrangement,reduce the average day in hospital and costs and improve the utilization rate of operating rooms,and peed up the operation turnover effect.Methods Retrospective analysis in November 2009 to December 2010 in our hospital were implemented,and 480 cases of LC/DS and the same period 476 regular routine LC surgery patients clinical data were collected.Time in hospital,cost and patient satisfaction were compared between the two groups.A day of surgical operation was arranged according to the operation types,characteristics and operative arrangement procedure was summarized and improved.Operation was arranged effect,fast,and safe.Results The mean time in hospital in LC/DS was(1.6 ± 0.6)d and(4.8 ±0.9)d in LC group,and the difference was statistically significant(t =-4.819,P ＜0.01).cost in hospital in LC/DS group was(8028 ±338)and(9016 ±266)yuan in LC group,and the difference also reached the statistical significance(t =-3.109,P ＜ 0.01).Conclusions Reasonable coordinating of a day of surgery can effectively improve the operating room efficiency,speed up the turnover of surgery patients.Patients average day in hospital was shorten.It takes great economical benefit both for patients and hospital.

OBJECTIVETo compare therapeutic effects of herb-partitioned spread moxibustion and western medicine on chronic nonspecific ulcerative colitis.

METHODSSixty cases were randomly divided into a spread moxibustion group (n = 28) and a western medicine group (n = 32). The spread moxibustion group was treated with herb-partitioned spread moxibustion at lower limb around stomach meridian, abdomen region around Guanyuan (CV 4) and lower Jiaji (EX B 2) points; and the western medicine group was treated with oral administration of Sulfasalazine. Their therapeutic effects were observed after treatment.

RESULTSThe cured-markedly effective rate was 71.4% (20/ 28) in the spread moxibustion group, and 25.0% (8/32) in the western medicine group, the former was better than the latter (P < 0.05).

CONCLUSIONSThe therapeutic effect of herb-partitioned spread moxibustion for treatment of chronic nonspecific ulcerative colitis is better than that of the oral administration of Sulfasalazine with less adverse reaction, and is worth popularizing in clinic.

Acupuncture Points ; Adult ; Chronic Disease ; therapy ; Colitis, Ulcerative ; drug therapy ; therapy ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Male ; Meridians ; Middle Aged ; Moxibustion ; Young Adult

AIMTo study the mechanism of butylated hydroxyanisole-induced neural differentiation of fetal liver cells in vitro.

METHODS14.5-day-old mouse fetal liver-derived cells were cultured, and were induced by 200 micromol/L butylated hydroxyanisole (BHA) combined with PI3K inhibitor LY294002 (20 micromol/L), and then were incubated in serum-free medium. Expression of genes in treated or untreated cells were assayed by Western blotting or RT-PCR.

RESULTSThere was low level of neurofilament-L (NF-L) and brain factor-1 (BF-1) but no neurofilament-H (NF-H) and tyrosine hydroxylase (TH) in fetal liver cells. BHA promoted significantly expression of neuron-specific NF-L, NF-H, BF-1, and TH in fetal liver cells. NF-L mRNA increased 5.8 fold, NF-H mRNA 8.0 fold, BF-1 mRNA 2.68 fold, and TH mRNA 30 fold, respectively (all P < 0.01 vs untreated cells). NF-L protein increased 11.29 fold, NF-H 5.5 fold, BF-1 2.53 fold, TH 4.76 fold. Moreover, expression of these BHA-induced genes were inhibited by PI3K inhibitor LY294002.

CONCLUSIONBHA induced neural differentiation of fetal liver cells through PI3K.

Animals ; Butylated Hydroxyanisole ; pharmacology ; Cells, Cultured ; Embryo, Mammalian ; cytology ; Hepatocytes ; drug effects ; metabolism ; Mice ; Mice, Inbred C57BL ; Nerve Tissue Proteins ; biosynthesis ; genetics ; Phosphatidylinositol 3-Kinases ; metabolism

Objective To evaluate the safety of dabigatran etexilate in the anticoagulant therapy.Methods The randomized controlled trials about dabigatran etexilate in the treatment of all kinds of embolism disea-ses, which had been published from the time of library foundation to June, 2015 were collected from PubMed, ClinicalTrials.gov, CNKI data-base according to the following criterias.An the same time results of studies were analyzed through using Stata 13.1 software and Rev Man 5.3 software.Results Sixteen randomized controlled trials were includ-ed, involving 42395 patients.Meta -analysis showed that dabigatran etexilate could significantly reduce the incidence of major bleeding com-paring with comparators ( P<0.05 ) , especially compared with warfarin ( P<0.01 ) .However the incidence of major bleeding with dabigatran etexilate was similar to comparators when the dosage was increased. Moreover dabigatran etexilate could significantly increase the risk of myo-cardial infarction ( P <0.01 ) , especially compared with warfarin (P<0.01).while the incidence of myocardial infarction with dabigatran etexilate was increased with the increase of the dosage.In addition dabig-atran etexilate could significantly increase the incidence of gastrointestinal bleeding ( P <0.01 ) , especially compared with warfarin and placebo ( P<0.05) , which was dose-dependent.There was no publication bias in funnel plots and sensitivity analysis indicated the results were reliable. Conclusion The use of dabigatran etexilate was associated with a significant increase in the risk of myocardial infarc-tion and gastrointestinal bleeding.Therefore, clinicians need to take these risks into account in treatment.

Encephalomyocarditis virus (EMCV) is a natural epidemic zoonotic pathogen. However, no reports have been published regarding the isolation, identification and full-length genome of EMCV from a local aardvark population. In present study, an EMCV isolate HNXX13 was isolated from aardvarks named Huainan-pig in Henan Province. The systematic identification, full-length genome sequencing and molecular characteristic analysis of the isolate HNXX13 were conducted. The result showed that the isolate was spherical with a diameter of 24-30 nm, neither heat- nor acid-resistant, sensitive to trypsin, insensitive to chloroform, not protected by bivalent cationic, and the specific fluorescence was observed in the cytoplasm of BHK-21 cells infected with the isolate by using indirect fluorescence assay. The full-length genome of EMCV HNXX13 generated a 7 725bp sequence (GenBank: F771002), with 81.0%-99.9% nucleotide identity to reference strains from different animals, and 99.5% with a Chinese reference strain isolated earlier from a commercial pig herd. The phylogenetic tree based on the full-length genome and ORF sequences identified that all EMCV strains were divided into three groups G1, G2 and G3, and strain HNXX13 belonging to the G1 group with other Chinese reference strains. The result also identified that this EMCV infection could cause severe clinical signs in a local aardvark population, and enriches the molecular epidemiological data of EMCV in China. Regional differences exist in EMCV genome and transmission is limited within a certain area. However, the cross-infection and transmission of EMCV between aardvark and mice appears most likely. Mutations have occurred in some amino acids of EMCV strain HNXX13 during the transmission in local aardvark herd and these mutations might make the virus easier to infect the aardvark.
Animals ; Animals, Wild ; virology ; Cardiovirus Infections ; veterinary ; virology ; China ; Encephalomyocarditis virus ; classification ; genetics ; isolation & purification ; Genome, Viral ; Mice ; Molecular Sequence Data ; Phylogeny ; Xenarthra ; virology

Fucoidan is one of the main bioactive components of polysaccharides. The current study was focused on the anti-tumor effects of fucoidan on human heptoma cell line HepG2 and the possible mechanisms. Fucoidan treatment resulted in cell cycle arrest and apoptosis of HepG2 cells in a dose-dependent manner detected by MTT assay, flow cytometry and fluorescent microscopy. The results of flow cytometric analysis revealed that fucoidan induced G2/M arrest in the cell cycle progression. Hoechst 33258 and Annexin V/PI staining results showed that the apoptotic cell number was increased, which was associated with a dose-dependent up-regulation of Bax and down-regulation of Bcl-2 and p-Stat3. In parallel, the up-regulation of p53 and the increase in reactive oxygen species were also observed, which may play important roles in the inhibition of HepG2 growth by fucoidan. In the meantime, Cyclin B1 and CDK1 were down-regulated by fucoidan treatment. Down-regulation of p-Stat3 by fucoidan resulted in apoptosis and an increase in ROS in response to fucoidan exposure. We therefore concluded that fucoidan induces apoptosis through the down-regulation of p-Stat3. These results suggest that fucoidan may be used as a novel anti-cancer agent for hepatocarcinoma.

OBJECTIVETo improve the accuracy and sensitivity of cell membrane chromatography (CMC) and evaluate the feasibility of CMC in the study of subtype receptors.

METHODSPlasmids were used to transfer alpha(1B)-AR cDNA into human embryonic kidney 293 (HEK293) cell lines to obtain cell lines stably overexpressing the subtype receptors. HEK293 alpha(1B) cell membrane stationary phase (CMSP) was prepared by immobilizing the cell membrane on silica. The retention time of 9 alpha(1)-adrenoceptor ligands and capacity factors(kappa'(HEK293 alpha1B)) were calculated. The capacity factors of rat liver tissue and primary cultured rat hepatocytes were also calculated for a correlation analysis.

RESULTSThe calculated capacity factors (kappa') were positively correlated to the published pKi values. The affinity rank orders were identical. The longest retention of the 9 alpha(1)-adrenoceptor ligands occurred on CMSP prepared with HEK293 alpha(1B) cell lines, while CMSP obtained from rat liver tissue showed the shortest retention of the ligands.

CONCLUSIONCMC proves practical in the study of the subtype adrenoceptors. The accuracy and sensitivity of CMC can be improved using HEK293 alpha(1B) cell membrane.

Animals ; Cell Membrane ; metabolism ; Chromatography, Affinity ; methods ; DNA, Complementary ; metabolism ; Female ; HEK293 Cells ; Humans ; Kidney ; cytology ; embryology ; Ligands ; Male ; Rats ; Rats, Sprague-Dawley ; Receptors, Adrenergic, alpha-1 ; metabolism ; Sensitivity and Specificity

Objective To assess the changes and the leading cause of deaths for children under 5 years old,in China,during 2000-2010,with the aim of evaluation on the progress in achieving the relative goal set by "National Program of Action for Child Development in China (2001-2010)",and understanding the related challenges.Methods Data used in this study were collected from the population-based National Maternal and Child' s Health Surveillance Network of China.Infant Mortality Rate (IMR),Under-5-mortality rate (U5MR) and the leading cause of deaths for under-5 children were analyzed.Results Nationwide IMR and U5MR in 2010 dropped by 59.3％and 58.7％ respectively,compared to that in 2000.Decreases by 50.8％ and 47.1％ in IMR and U5MR were observed in urban areas,and 56.5％ and 56.0％ in rural areas during this period.Compared with data from 2000,the leading causes-specific U5MR in 2010 had significantly declined.The top 5 leading causes of death in 2010 were premature birth/low birth weight,pneumonia,birth asphyxia,congenital heart disease and accidental suffocation,but were different in urban and rural areas.In 2010,both IMR and U5MR from the rural areas were 2.8-folds than that of the urban areas.In addition,IMRs in the Middle and Western parts of China were 1.5 and 2.3-folds respectively of that in the East,and U5MR in Middle and West was 1.5 and 2.2-folds respectively of that in East.Conclusion IMR,U5MR and the leading causes specific mortality rate in China declined remarkably from 2000 to 2010,and the goal set by "National Program of Achon for Child Development in China (2001-2010)" had been successfully achieved.However,the disparity on child' s health in regions and in urban or rural areas,still remained a challenge.

OBJECTIVETo isolate, culture and identify a dog periodontal ligament stem cells (PDLSC) line in vitro.

METHODSThe adult dog periodontal ligament cells were isolated by limited dilution of culture cell for single cell clone. Cells originated from one of these clones were assessed through colony-forming efficiency and immunocytochemistry assay and alkaline phosphatase stain was used to identify the source of adult dog periodontal stem cells, at the same time, PDLSC were induced with mineralizatin solution and was found to have long protrude like an osteoblast. Differentiation of PDLSC were assessed. Mineralized potential was studied by Von-Kossa staining.

RESULTSThe dog PDLSC expressed STRO-1, which was the marker of mesenchymal stem cells. Also Vimentin, osteoblast-like marker alkaline phosphatase and Collagen-I expressed weakly. Cells were clonegenic, highly proliferative cells and capable of differentiating into osteoblasts/cementoblasts.

CONCLUSIONThe evidence suggests that the cultured cells were stem cells from adult dog periodontal ligament.

Adult ; Adult Stem Cells ; Alkaline Phosphatase ; Animals ; Cell Culture Techniques ; Cell Differentiation ; Cells, Cultured ; Dental Cementum ; Dogs ; Humans ; Mesenchymal Stromal Cells ; Osteoblasts ; Periodontal Ligament ; Stem Cells