OBJECTIVE: To study the effect of cinobufacini injection on proliferation, migration and tube-like structure formation of human hepatoma HepG-2 cells co-cultured with human lymphatic endothelial cells. METHODS: Co-culture system of human hepatoma HepG-2 cells and human lymphatic endothelial cells is established by means of transwell chamber; cell growth curve is used to observe the effect of cinobufacini injection on the proliferation of HLEC co-cultured with HepG-2 cells; migration assay is used to observe the effect of cinobufacini injection on the migration of HLEC co-cultured with HepG-2; matrigel assay is used to observe the effect of cinobufacini injection on the tube-like structure formation of HLEC co-cultured with HepG-2 cells. RESULTS: Cinobufacini injection significantly inhibits proliferation(P < 0.05), migration (P < 0.05) and tube-like structure formation (P < 0.05) of HLEC co-cultured with HepG-2 cells in dose dependent ways. CONCLUSION: Cinobufacini injection inhibits proliferation, migration and tube-like structure formation of HLEC co-cultured with HepG-2 cells, which might contribute the inhibiting mechanisms of cinobufacini injection on tumor metastasis.

Objective To observe the efficacy of prophylactic use of gramsetron(Gra) combined with dexamethasone(DEX) on preventing postoperative nausea and vomiting(PONV) after umbilical one-trocar laparoscopic appendectomy(UOTLA)in children.Methods Sixty children requring UOTLA under general anesthesia were allocated randomly into 3 groups,and each of group included 20 children.Group saline(C),group Gra 40 ?g/kg,group Gra 40 mg/kg and group DEX 0.2 mg/kg(Gra+DEX) were respectively injected intravenously after appendectomy performed outside the abdominal cavity during the operation.Results The incidence of PONV decreased significantly in group Gra and group Gra+DEX than that in group C(Pa

Objective:To compare psychosocial factors and immune function between patients with breast cancer and benign tumor before operation.Methods:The patients of breast tumor(56 case)were measured by clinical psychological rating scale(EPQ、HAMD、HAMA、LES、SSRS、DSQ)before operation,and their serum levels of IgA, CD_4~+,CD_(19)~+ and the activity of NK cell were measured.The patients of breast tumor were divided into breast cancer group(36 case)and benign tumor group(22 case)bases on results of pathology.Results:In breast cancer group, there were significantly higher scores of neurotic,total and negative life events,immature defense styles,HAMA and HAMD than those in benign tumor group;while NK cell activity,levels of CD_4~+ and CD_(19)~+ and the score of mature defense,social supports,subjective supports and utility degree of supports were marked lower.There were significantly correlation between immune function(NK cell activity,content of CD_4~+,CD_(19)~+,IgA)and psychosocial factors(social support,P score of EPQ,HAMD and HAMA total score,quantity of negative life events etc). Conclusion:Cancer patients had more server psychosocial problems and lower immune function.The immune function of patient with breast cancer before operation is associated with the various psychosocial factors.

Fermentation conditions for the production of spores of Bacillus mucilaginosus mutant 021120 were optimized through the single factor and orthogonal experiments.The results showed that the biomass and formation of spores were obviously affected by carbonate,followed by nitrogen.Addition of CaCO3 and enhancing air flux notably promoted the formation of spores.The optimal culture medium was composed of 2% starch,0.4% Yeast,0.1% K2HPO4,0.1% MgSO4?7H20 and 0.5 %CaCO3,pH7.5.6% of the inoculum prepared with two-stage extensive culture was inoculated into a 70L fermentor.The fermentation was carried out with 2.0~2.5 vvm of air flux at 32℃ for 38~42h,and the spores of 9.80?108 cfu ml-1 was obtained.Under these optimal fermentation conditions,the capsule was successfully controled and the formation of the spores was effectively improved,thus the satisfying bacteial product was obtained.

0.05). ConclusionThe expression of VOCC mRNA and BKCa mRNA in kidney tissues of IgA nephropathy patients are abnormal.There is positive correlation between the abnormal expression of VOCC mRNA and BKCa mRNA and total glomerular pathological lesions integrals.The expression of VOCC mRNA and BKCa mRNA in kidney tissues of IgA nephropathy may serve as the indictor for the disease progression.

Adolescent ; Adult ; Dermatitis, Occupational ; diagnosis ; Eye Diseases ; diagnosis ; etiology ; Female ; Humans ; Male ; Trichloroethylene ; poisoning ; Young Adult

Objective To investigate the expression in the VEGF,TGF-?1 of cervical squamous car- cinoma infected by HPV16,18.Methods Cells exfoliated from cervix(collected by clinician)of 99 women with cervical cancer and 54 women as a control group were analyzed blindly by human papillomavirus type 16 and 18 Fluorescent Polymerase Reaction Diagnositic kit.The expression of VEGF,TGF-?1 of the positive HPV16,18 of 38 women with cervical squamous cancer were studied by immunohistochemical stain.Results The positive expression of HPV16,18 was observed in 53 in the case of cervical cancer with positive rates of 54 %,but the positive rates was 7 % in the control group(P

Forty type 2 diabetic patients with diabetic peripheral neuropathy (DPN) were assigned to two groups and treated respectively with?-lipoic acid or mecobalamin for 2 weeks.The results suggested that?-lipoic acid could accelerate the nerve conduction velocity and decrease the plasma level of endothelin and C reactive protein as well as microalbuminceria with a effect similar to mecobalamin therapy on DPN.

Objective To evaluate the relationship between combined muhigene detection and response to chemotherapy and prognosis in epithelial ovarian carcinomas(EOCS).Methods A total of 80 ovarian tissue samples taken from the surgical specimens of patients with EOCS of our hospital in the last two decades who had received chemotherapy "after surgery were paraffin-embedded.The samples were divided into 2 groups,good prognosis group(patients who survived more than 2 years,n=46)and poor prognosis group(patients who survived less than 2 years,n=34).The expression levels of ToPo-Ⅱ,Ki-67,MGMT, PCNA,p27,p53,pl6,P-gp,LRP,GST-?,bcl-2,C-myc,Fas,bax,MSH2,MRP and BCRP were investigated by the combination of tissue arrays and immunohistoehemical streptavidin-biotin peroxidase(SP) method in all samples.Data were analysed with SPSS 12.0 for windows.Results There were statistically significant differences in the positive expression levels of P-gp,BCRP,MGMT,MSH2,p27 and p16(62%, 50% and 50% in poor prognosis group vs 33%,28% and 28% respectively,P

ObjectiveTo explore the role of Network Management System (NMS) in decreasing mortality and incidence of cerebral palsy in preterm infants.MethodsThe data of 356 preterm infants transported by NMS from January 2004 to December 2005 were analyzed.ResultsNo death cases occurred during the transportation of 356 preterm infants, the success rate was 100%. 292 cases (84.39%) were cured and 36 cases (10.4%) were effective. 7 case dead for compliance, the mortality was 19.6‰. 3 cases suffered from cerebral palsy , the incidence of cerebral palsy was 8.6‰.ConclusionNMS applied to preterm infants is a high-effective medical model, and plays an important role in improving the forward prognosis of preterm infants.