Adolescent ; Child ; Female ; Humans ; Male ; Nasal Mucosa ; metabolism ; Nasal Polyps ; metabolism ; Neuropeptides ; classification ; metabolism

Pericytes are a very important cellular constituent of the blood-brain barrier.They play a regulatory role in brain angiogenesis,endothelial cell tight junction formation,blood-brain barrier differentiation,microvascular dynamic motion and structural stability.Pericytes exhibit unique functional characteristics in some diseases,such as cerebrovascular disease,neurodegenerative disease,neuroimmune disease and traumatic brain injury.This article reviews the roles of pericytes in the blood-brain barrier.

The use of optimal regulatory sequences for simultaneous expression of the transgenes might play a significant role in engineering plants with increased disease and insect resistance.The plant expression vector pOMS-GUS,which contained the GUS gene under the control of a chimeric promoter based upon the mannopine synthase(mas)promoter and the octopine synthase(ocs)enhancer,was constructed.Used as control,another vector pMAS-GUS,carried the GUS gene driven by only the mas promoter.The two vectors were introduced into tobacco plants by Agrobacterium-mediated transformation.Fluorometric assays for GUS activity and reverse transcription-polymerase chain reaction(RT-PCR)analysis revealed that GUS gene expressed weakly with untreated transgenic tobacco while the level of GUS activity increased steadily after 1 h subjected to wounding.The expression of the mas and ocs/mas promoters was induced a further 1.8-fold and 5.7-fold,respectively.SA(1 mmol/L)or MJ(250 ?mol/L)treatment also caused a large induction of the ocs/mas chimeric promoter;And the application of SA in combination with MJ(1 mmol/LSA & 250 ?mol/L MJ)produced an additive effect that exceeded the wounding response.The results showed that the ocs/mas chimeric promoter is a strong inducible promoter that can be activated by various stresses.The chimeric promoter should have utility in development of disease and insect resistant transgenic crops.

This paper reported a new home-made nonpoisonous substrate of horseradish peroxidase(HRP),3,3′,5,5′-tetramethylbenzidine sulphate(TMBS),used in ELISA with the aqueous soluble egg antigen(SEA)as antigen;proved patients'sera as antibo- dy and the antihuman IgG was replaced by protein A conjugate. The optimun condition obtained by orthogonal design was 1.0 millimolarity of TMBS,1.2 millimolarity of H_2O_2,and pH value at 5.0.The best time to stop reaction with H_2SO_4,was 30-60 min after adding substrate TMBS.At the same time,TMB and OPD were used as compared substrates.It showed that the reaction colour of both TMBS and TMB remained much more stable than that of OPD when being exposured in light and oxygen.After the solutions of TMBS and TMB were stored under 4℃ in refrigerator for 60 days,the coefficient of variation among different experiments was less than 10%. The results in detecting specific antibody in sera from 28 patients with proved schistosomiasis also showed that the sensitivity of TMBS was remarkably higher than OPD.It was found that TMBS used in enzyme immunoassay for demonstrating specific antibody was suitable not only for optical density(OD)read by spectrophotometer but also for estimation judged by naked-eye.During TMBS and TMB used in indirect immunoperoxidase method(IIP),the specific antibody in the tissue can be easily dete- cted and located as well.

Objective To explore the relationship of etiopathogenesis,therapy and prognosis in children with chronic arrhythmia.Met-hods Forty cases aged 48 h to 12 years old between Sep.2003 to Oct.2006 were selected.All children had vertigo,syncope,Adam-Stoke syndrome,cardiac shock.Some cases were detected with complete atrioventricular block(CAVB)(33 cases),sinus bradycardia(5 cases) or sick sinus syndrome(2 cases) by electrocardiogram(ECG) at least 2 times,and these symptoms lasted 3 days at least.Congenital CAVB children having no clinical symptom were given no treatment.Cases having sinus bradycardia,sick sinus syndrome resulted from viral myocarditis,the earlier were given antiviral medicine,energy composition,intravenous megavitamin C,isoprenaline,and large dose of adrenocortcal hormone simultaneously.Some were given human ?-globulin.Eight of them were set temporary pacemaker because of unsatisfactory treatment,and 2 of them were set permanent pacemaker.Results Twenty-eight cases were cured,9 cases were improved and 3 cases died.Six out of the cured cases and improved cased were set permanent or temporary pacemaker.Conclusions Early discovery,early diagnosis,and early temporary pacemaker use will lead a better prognosis in regular treatment.On the contrary,the delayed treatment on children with viral myocarditis and CAVB caused by dilated cardiomyopathy will lead a negative prognosis.

Apnea ; epidemiology ; pathology ; therapy ; Birth Weight ; China ; epidemiology ; Female ; Gestational Age ; Humans ; Infant, Newborn ; Infant, Very Low Birth Weight ; Male ; Risk Factors ; Time Factors

Humans ; Infant, Newborn ; Male ; Protein C ; metabolism ; Protein C Deficiency

Angiopoietins ; metabolism ; physiology ; Animals ; Cell Proliferation ; Endothelial Cells ; cytology ; physiology ; Humans ; Neoplasms ; blood supply ; Neovascularization, Pathologic ; physiopathology ; Neovascularization, Physiologic ; physiology ; Pericytes ; cytology ; metabolism ; physiology ; Proto-Oncogene Proteins c-sis ; metabolism ; physiology ; Receptor, Platelet-Derived Growth Factor beta ; metabolism ; physiology ; Receptor, TIE-2 ; metabolism ; physiology ; Signal Transduction

Carcinoma, Renal Cell ; complications ; Humans ; Kidney Neoplasms ; complications ; Lymphoma, Non-Hodgkin ; complications ; Male ; Middle Aged

Chondrodysplasia Punctata ; congenital ; Humans ; Infant, Newborn ; Male