1.The biological features and their roles of cancer stem cells in invasion and neovascularization of cancer.
Chinese Journal of Pathology 2009;38(8):505-506
Animals
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Apoptosis
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Cell Cycle
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Cell Differentiation
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Cell Proliferation
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Connexin 43
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metabolism
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Humans
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Neoplasm Invasiveness
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pathology
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physiopathology
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Neoplasms
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metabolism
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pathology
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physiopathology
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Neoplastic Stem Cells
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metabolism
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pathology
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physiology
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Neovascularization, Pathologic
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metabolism
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pathology
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physiopathology
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Receptors, CXCR4
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metabolism
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Receptors, Formyl Peptide
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metabolism
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Vascular Endothelial Growth Factor A
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metabolism
2.Recent advances in studies of tumor angiogenesis: heterogeneity of tumor microvascular architecture phenotype.
Chinese Journal of Pathology 2006;35(3):129-131
Antigens, CD34
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genetics
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metabolism
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Capillaries
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metabolism
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pathology
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Genetic Heterogeneity
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Microcirculation
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Neoplasms
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blood supply
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Neovascularization, Pathologic
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genetics
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metabolism
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pathology
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Phenotype
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Platelet Endothelial Cell Adhesion Molecule-1
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genetics
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metabolism
3.Tumor associated stem/progenitor cells in tumorigenesis and progression of cancer.
Chinese Journal of Pathology 2011;40(3):145-146
Animals
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Cell Proliferation
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Disease Progression
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Endothelial Cells
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pathology
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Humans
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Indoleamine-Pyrrole 2,3,-Dioxygenase
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metabolism
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Mesenchymal Stromal Cells
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immunology
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metabolism
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pathology
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Neoplasms
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immunology
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pathology
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Neoplastic Stem Cells
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pathology
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Neovascularization, Pathologic
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pathology
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Stem Cells
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pathology
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T-Lymphocytes
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immunology
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pathology
5.Updates on study of glioma stem cells.
Zhi-hua ZHOU ; Liang YI ; Xiu-wu BIAN
Chinese Journal of Pathology 2007;36(3):201-203
AC133 Antigen
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Animals
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Antigens, CD
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metabolism
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Cell Differentiation
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Cell Proliferation
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Drug Resistance, Neoplasm
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Glioma
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pathology
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Glycoproteins
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metabolism
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Humans
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Neoplastic Stem Cells
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metabolism
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pathology
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physiology
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Neovascularization, Pathologic
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etiology
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pathology
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Peptides
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metabolism
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Radiation Tolerance
6.Effects of nordihydroguaiaretic acid on the expression of GFAP gene of human malignant glioma cell line SHG-44
Li YAO ; Xiu-Wu BIAN ; Zi-Qiang CHEN
Journal of Third Military Medical University 2001;23(3):254-256
Objective To investigate the expression of glial fibrillary acidic protein (GFAP) gene and its significance in the process of glioma cell differentiation induced by nordihydroguaiaretic acid (NDGA). Methods Immunohistochemistry (IHC) and in situ hybridization were used to detect the expression changes of GFAP protein and GFAP mRNA qualitatively and quantitatively. Results The expression levels of GFAP protein and GFAP mRNA in NDGA treatment group were significantly increased compared with the control group (P<0.01). Conclusion NDGA could induce GFAP gene in malignant glioma cells and the up-regulation of this gene expression might be one of the mechanisms by which NDGA induces glioma differentiation.
7.Quantitative study on morphologic features and proliferative activity during DEN-induced hepatocarcinogenesis in rats
Xin-Li ZHANG ; Jing-Quan SHI ; Xiu-Wu BIAN
Journal of Third Military Medical University 2001;23(3):304-307
Objective To explore the relationship between morphologic evolution and proliferative activity during hepatocarcinogenesis in rats. Methods Imaging analysis technique was used to detect the morphologic parameters of cells in hepatic lesions in both Solt-Farber model and diethylnitrosamine (DEN)-induced liver cancer model. Immunohistochemistry was employed to detect proliferating cell nuclear antigen (PCNA) and bromodeoxyuridine (BrdU). Results The oval cells were identified as irregular small proliferating cells in size of one-eighth of and with a nucleus/cytoplasm ratio of 6 times of the normal hepatocyte by image analysis. The morphometric parameters of basophil hepatocyte in precancerous foci and nodule were similar to those of the liver cancer cell. PCNA and BrdU positive cells were mainly localized within the proliferative foci and hepatocellular carcinoma (HCC) tissues. There was a better consistency between the development of hepatic lesions and cellular proliferative activity. Conclusion The morphologic evolution is closely related to proliferative activity during hepatocarcinogenesis in rats.
8.Expression of some oncogenes and point mutation of c-Ha-ras1 during hepatocarcinogenesis in rats
Xin-Li ZHANG ; Jing-Quan SHI ; Xiu-Wu BIAN
Journal of Third Military Medical University 2001;23(3):308-311
Objective To study the expressions of oncogenes c-Ha-ras, c-ki-ras, pan-ras and c-myc and point mutation of c-Ha-ras1 during hepatocarcinogenesis in rats. Methods Immunohistochemistry, in situ hybridization and microdissection of tissue (MDT)-PCR-SSCP were used to detect the oncogene expressions and point mutation of c-Ha-ras1 in both Solt-Farber model and DEN-induced liver cancer model. Results The overexpression of c-Ha-ras was closely associated with the formation and proliferation of the precancerous basophilic hepatocyte foci, while that of c-myc with the growth of the oval cell foci. The abnormalities of IGF-Ⅱ played an important role in the evolution of precancerous foci/nodules towards hepatocellular carcinoma (HCC). The overexpression of fms was only associated with HCC of some rats. Conclusion Hepatocarcinogenesis in rats was related with the overexpression of c-Ha-ras, c-myc, IGF-Ⅱand fms and the point mutation of c-Ha-ras1, and overexpression of these oncogenes was associated with morphological evolution.
9.Effect of nordy on biological behaviors of malignant glioma cell line U87MG and the analysis of differential expression proteome.
Jian-ping XU ; Hong LIU ; Xiu-wu BIAN ; Jian-hong CHEN ; Xiang-dong ZHOU ; Yu-zhang WU
Chinese Journal of Pathology 2007;36(9):609-613
OBJECTIVETo explore effects of nordy on biological behaviors of human malignant glioblastoma cell line U87MG in vitro and transplanted tumor in vivo, and to identify the differential proteome upon Nordy induced differentiation.
METHODSGlioblastoma U87MG cells were induced to differentiate by synthetic lipoxygenase inhibitor, Nordy. The drug was also given via peritoneal injection to nude mice (27 mg/kg body weight) bearing orthotopic transplanted tumors of U87MG cells in the brain. The tumor volumes and GFAP expression were measured. Total proteins of U87MG cells after Nordy treatment were analysed by two-dimensional gel electrophoresis. PDQuest 7.1 computer software was used to compare protein profiles of the treated cells with that of untreated control. Differentially expressed proteins were then selected and characterized by matrix assisted laser desorption ionization-time of flight-mass spectrometry. The functional aspects of these proteins were analyzed by bioinformatics.
RESULTSNordy suppressed both the proliferation of U87MG cells in vitro and the tumor growth of orthotopic transplanted tumors in vivo (P < 0.01). The differentially expressed proteins induced by Nordy included proliferation-associated gene A, alternative splicing factor ASF-3, eukaryotic translation initiation factor 5A, coffilin 1 (non-muscle), beta galactoside binding lectin, glyceraldehyde-3-phosphate dehydrogenase, enolase 1 and an unknown protein.
CONCLUSIONSNordy promotes the differentiation of glioblastoma cells, by which it may serve as a therapeutic agent. Various proteins identified during Nordy-induced differentiation are involved in the cell proliferation, metabolism, differentiation, apoptosis and gene transcription.
Animals ; Antineoplastic Agents ; pharmacology ; Brain Neoplasms ; metabolism ; pathology ; Cell Differentiation ; Cell Line, Tumor ; Cell Proliferation ; Female ; Gene Expression Regulation, Neoplastic ; Glial Fibrillary Acidic Protein ; metabolism ; Glioblastoma ; metabolism ; pathology ; Humans ; Lipoxygenase Inhibitors ; pharmacology ; Male ; Masoprocol ; analogs & derivatives ; pharmacology ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Neoplasm Transplantation ; Protein Array Analysis ; Proteome ; genetics ; metabolism ; Proteomics ; methods ; Random Allocation ; Tumor Burden
10.VEGF-induced tubulogenesis of endothelial cells from human brain malignant glioma in the three dimentional model.
Xue-feng JIANG ; Jin-si BAI ; Xiu-wu BIAN ; Jia-you LU ; Wen ZHAO ; Jing-quan SHI
Chinese Journal of Pathology 2005;34(9):579-582
OBJECTIVETo compare the tubulogenesis capability of malignant glioma-derived microvessel endothelial cells (GDMEC) from human brain with that of ECV304 cells in a three dimentional model and to explore the significance of GDMEC in the study on angiogenesis.
METHODSThe GDMEC were isolated from malignant gliomas of human brain and purified by selective binding to the monoclonal antibody against CD105 bound to the magnetic MACS MicroBeads. GDMEC and endothelial-like cell line ECV304 were compared with their capabilities of formatting tubule-like structure (TLS) in the three dimentional collagen matrix, with or without inducement by various concentration of vascular endothelial growth factor (VEGF).
RESULTSThe obtained GDMEC had a high purification (98%) and could be successfully cultured in vitro. GDMECs formed more TLS than ECV304 cells of the same number and at the same time points. VEGF could induce rapid formation of TLS in a dose-dependent manner, however, ECV304 cells were less response to VEGF stimulation.
CONCLUSIONSGDMEC could maintain their endothelial characteristics and potential capability of angiogenesis. They were more response to VEGF than ECV304, therefore, more suitable for in vitro studies on tumor angiogenesis.
Brain Neoplasms ; blood supply ; Cells, Cultured ; Dose-Response Relationship, Drug ; Endothelial Cells ; drug effects ; Endothelium, Vascular ; cytology ; Glioma ; blood supply ; Humans ; Immunomagnetic Separation ; Microcirculation ; pathology ; Neovascularization, Pathologic ; Vascular Endothelial Growth Factors ; administration & dosage ; pharmacology