Objective To investigate the clinical and mammographic features of plasma cell mastitis.Methods Twenty-five patients(28 lesions)with histologically confirmed plasma cell mastitis, aged from 26 to 70 years(mean age 41 years),were examined with X-ray mammography.The clinical manifestations and imaging features were retrospectively reviewed.Results No case was in lactation.The painful irregular masses,ranged from 1.3 to 8cm in size,were found in 22 patients,while 3 patients with acute episode.Recurrent episodes of breast masses were noted in 4 patients.Based on the mammographic appearances,the plasma cell mastitis were classified as the following four types:inflammation-like type (2/28),ductal ectasia type(3/28),focal infiltration type(10/28)and nodular type(13/28).The valuable radiogyaphic signs:(1)An asymmetrically increased density along the lactiferous duct with a flame-like appearance,inhomogeneous low density tubular structures and scattered stick-shape calcifications.(2) Architectural distortion and oil cysts formation in adjacent area,(3)Subareolar ductal ectasia.Conclusions The clinical and mammographic characteristics of plasma cell mastitis are critical to avoiding unnecessary surgery.Histopathological result is needed for the diagnosis in patients highly suspected of malignancy.

In order to effectively remove the invalid impurities in Tongan injection, optimize the optimal parameters of the impurity removal technology of liquid mixing process, in this paper, taking Tongan injection as the research object, with the contents of celandine alkali, and sinomenine, solids reduction efficiency, and related substances inspection as the evaluation indexes, the removal of impurities and related substances by the combined process of refrigeration, coction and activated carbon adsorption were investigated, the feasibility of the impurity removal method was definited and the process parameters were optimized. The optimized process parameters were as follows: refrigerated for 36 h, boiled for 15 min, activated carbon dosage of 0.3%, temperature 100 degrees C, adsorption time 10 min. It can effectively remove the tannin, and other impurities, thus ensure the quality and safety of products.
Adsorption ; Charcoal ; chemistry ; Drug Compounding ; instrumentation ; methods ; Drug Contamination ; prevention & control ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Quality Control

OBJECTIVETo investigate the elastic limit and relevant enclasp force of the non-precious metal casting clasp.

METHODSCasting clasp samples of five cobalt-chromium alloys and one 18 - 8 nickel-chromium alloy were made from prefabricated clasp wax by invesing, casting, sandblasting, and ultrasonic cleaning. The process of casting clasp samples deflected by loading and returned by unloading was tested and electric signals were collected by an omnipotent material machine. The analog electric signal was converted to digital signal by an analog to digital converter and stored in a computer. The elastic limit and the relevant enclasp force were analyzed using a relative software.

RESULTSThe elastic limit and the relevant enclasp force of the casting clasp made from the 18 - 8 nickel-chromium alloy were smallest and those of the clasps made from the cobalt-chromium alloys in various brands were different. The range of the elastic limit of the cobalt-chromium alloy casting clasp with the length of 5.0 mm in undercut was 0.28 mm-0.33 mm and the relevant enclasp force was 14.42 g-19.28 g.

CONCLUSIONSIn clinic, we should select the suitable undercut deepness wherein the cobalt-chromium alloy casting clasps, according to different brands of the casting alloy, undercut length, undercut slope, and the clasp thickness.

Chromium Alloys ; Cobalt ; Dental Alloys ; Dental Clasps ; Dental Stress Analysis ; Denture, Partial, Removable ; Elasticity ; Humans ; Nickel ; chemistry ; Stress, Mechanical

OBJECTIVETo identify the fibrinogen (Fg) gene mutations in a Chinese pedigree of congenital afibrinogenemia.

METHODSThe plasma Fg activity and protein of the proband and his family members were detected. Genomic DNA was isolated from the peripheral blood mononuclear cells. All the exons and exon-intron boundaries of fibrinogen gene were amplified by PCR and sequenced thereafter.

RESULTSTwo mutations, 7972 del G in FGB and T2543A in FGG, were found in the proband.

CONCLUSIONSFGG2543 is a polymorphism site, which lead to the polymorphism of gamma144 I/K. The G deletion at base 7972 of FGB contributes to the frameshift mutation after amino acid 419, resulting in the truncated beta chain without the terminal 27 amino acids. The latter may contributes to the pathogenetic mechanisms in Chinese congenital afibrinogenemia patients. The G deletion at base 7972 of FGB is identified for the first time.

Adult ; Afibrinogenemia ; congenital ; genetics ; metabolism ; Base Sequence ; Blotting, Western ; DNA Mutational Analysis ; Exons ; genetics ; Female ; Fibrinogen ; genetics ; Humans ; Introns ; genetics ; Male ; Mutation ; Pedigree ; Polymerase Chain Reaction

OBJECTIVETo study the mechanical properties of Co-Cr ceramic alloy after recasts.

METHODSCo-Cr ceramic alloy cast samples were prepared and recast for 3 times without adding any new Co-Cr ceramic alloy. The tensile strength, 0.2% yield strength, percentage of elongation, flexural strength, flexural modulus and Vickers hardness of each specimen were measured.

RESULTSBeing cast for different times, the Co-Cr ceramic alloy showed no significant differences on their tensile strength, 0.2% yield strength, percentage of elongation, flexural strength, flexural modulus and Vickers hardness.

CONCLUSIONCo-Cr ceramic alloy can be recast for 3 times at least, without decrease of the mechanical properties.

Alloys ; Ceramics ; Chromium Alloys ; Dental Stress Analysis ; Hardness ; Materials Testing ; Metal Ceramic Alloys ; Tensile Strength

OBJECTIVETo investigate the change of retentive forces of cast cobalt-chromium (Co-Cr) alloy clasp in cyclic fatigue test.

METHODSSamples of three types of cast Co-Cr alloy (Group A: Hardalloy; B: Regalloy™; C: Vera PDN™) clasps were fabricated and placed at undercut depths of 0.25 mm, 0.50 mm. The clasps were drawn from the model molar cyclicly to simulate 5 years of clinical use in an universal testing machine. Retentive force were record at 21 different time point for each clasp during the whole fatigue testing process. Data were subjected to ANOVA, Chi-square test and linear regression analysis.

RESULTSAll clasps showed decreasing retention during the cyclic fatigue test. Clasps engaged in 0.50 mm undercut depth exhibited greater initial retentive force [Group A: (8.714 +/- 1.104) N, B: (9.072 +/- 0.653) N, C: (9.588 +/- 1.980) N] as well as greater loss of retention [Group A: (4.408 +/- 0.662) N, B: (3.484 +/- 0.494) N, C: (3.290 +/- 1.484) N] at the end of the test than clasps engaged in 0.25 mm undercut did [initial forces were (7.940 +/- 0.357), (7.834 +/- 1.308) and (8.156 +/- 1.067) N for Group A, B, C, respectively; loss of retention were (2.444 +/- 0.736) N, (2.954 +/- 1.048) N and (1.832 +/- 1.180) N for group A, B, C, respectively]. Negative correlation was found between the clasp retention and the logarithm of cycling times.

CONCLUSIONSCo-Cr alloy cast clasp could provide adequate retentive force for 5 years of clinical use.

Chromium Alloys ; chemistry ; Dental Alloys ; chemistry ; Dental Casting Technique ; Dental Clasps ; Dental Stress Analysis ; Denture Retention

OBJECTIVETo analyze the changes of carotid-femoral and carotid-radial pulse wave velocity in patients with essential hypertension, and effects of age and levels blood pressure levels on these changes.

METHODSAutomatic pulse wave velocity (PWV) measuring system was applied to examine carotid-femoral PWV (CFPWV) and carotid-radial PWV (CRPWV) as the parameters reflecting central elastic large arterial and peripheral muscular medium-sized arterial elasticity respectively. 517 hypertensive patients aged 17-82 years (272 males and 245 females, mean age 52.0 +/- 13.0 years) and 118 healthy subjects aged 19-82 years (52 males and 66 females, mean age 54.2 +/- 13.8 years) were recruited in the study.

RESULTSCFPWV increased with age in both groups (P < 0.001), whereas CRPWV did not change significantly. CFPWV and CRPWV both increased with blood pressure. A stepwise multiple regression analysis demonstrated that age and systolic blood pressure were positively related to CFPWV independently, while diastolic blood pressure was significantly associated with CRPWV.

CONCLUSIONSAge and components of blood pressure exert different effects on elasticity of central elastic large artery and peripheral muscular medium-sized artery. Examining the change of large arterial elasticity has important clinical value in hypertensive patients.

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Blood Flow Velocity ; Blood Pressure ; Carotid Arteries ; physiopathology ; Case-Control Studies ; Elasticity ; Female ; Femoral Artery ; physiopathology ; Heart Rate ; Humans ; Hypertension ; physiopathology ; Male ; Middle Aged ; Pulse ; Radial Artery ; physiopathology ; Young Adult

OBJECTIVETo investigate the effect of Rhizoma Curcumae (RC), arsenite trioxide (As2O3) on proliferation ana signal transduction molecule in lens epithelial cell (LEC), in order to provide experiment evidence for prevention and treatment of after cataract.

METHODProliferation of cultured bovine LEC were induced by induced by recombinant human basic fibroblast growth factor (rhbFGF); Inhibitory rates of LEC proliferation induced by RC, As2O3 were detected by methyl thiazolyl tetrazolium (MTT); Inhibitory effects of expression of proliferating cell nuclear antigen (PCNA) induced by RC, As2O3 in LEC were assayed via flow cytometer (FCM); Concentrations of LEC calcium ([Ca2+]i) were determined by spectrofluoremeter, intracellular concentrations of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) of LEC were measured by radioimmunoassay.

RESULTInhibitory rates of RC, As2O3 on LEC proliferation induced by rhbFGF increased significantly, showing dose-dependent (P < 0.01). PCNA expression of LEC proliferation induced by rhbFGF were down regulated obviously by RC, As2O3, showing dose-dependent (P < 0.01). Concentrations of [Ca2+]and cAMP increased and cGMP decreased significantly in LEC of proliferation inhibited by RC, As2O3 (P < 0.01).

CONCLUSIONRC, As2O3 can inhibit LEC proliferation obviously. Signal transductions of [Ca2+]i, cAMP, cGMP may be the important molecular mechanism. There are broad prospect for RC, As2O3 on prevention and treatment of after cataract.

Animals ; Arsenicals ; pharmacology ; Calcium ; metabolism ; Cattle ; Cell Proliferation ; drug effects ; Cells, Cultured ; Curcuma ; chemistry ; Cyclic AMP ; metabolism ; Cyclic GMP ; metabolism ; Dose-Response Relationship, Drug ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Epithelial Cells ; cytology ; drug effects ; metabolism ; Fibroblast Growth Factor 2 ; genetics ; pharmacology ; Flow Cytometry ; Growth Inhibitors ; pharmacology ; Lens, Crystalline ; cytology ; drug effects ; metabolism ; Oxides ; pharmacology ; Proliferating Cell Nuclear Antigen ; metabolism ; Radioimmunoassay ; Recombinant Proteins ; pharmacology ; Rhizome ; chemistry ; Signal Transduction ; drug effects

OBJECTIVETo develop a new drug of treating the hepatitis C virus by studing the mechanisms of songzhi pills.

METHODThe four-wee old mice and two-month old rats were chosen to induce interferon.

RESULTThe contents of interferon among the groups treated with songzhi pills were significantly higher than those of the normal group and the model group (P < 0.01),

CONCLUSIONSSongzhi pills may have the function of inducing interferon.

Animals ; Drug Combinations ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Female ; Gardenia ; chemistry ; Interferon-gamma ; biosynthesis ; Male ; Mice ; Plants, Medicinal ; chemistry ; Polyporales ; chemistry ; Rats ; Rats, Sprague-Dawley

This study is to investigate whether the synthesized chiral compound Nordy has influence on the function of endothelial progenitor cells (EPCs) from human umbilical cord blood induced by vascular endothelial growth factor (VEGF). EPCs were isolated from human umbilical cord blood by density gradient centrifugation. After cultured for 7 -10 days, EPCs were prepared for detecting effect of Nordy on proliferation, migration and tubule-forming activity in Matrigel induced by VEGF. Incubation of EPCs with 100 micromol L(-1) Nordy for 24 h initially inhibited the proliferative capacity of EPCs induced by VEGF (P <0.05). Moreover, 25 -50 micromol L(-1) Nordy also exhibited inhibitory effect at 48 -72 h. In addition, 25 - 100 micromol L(-1) Nordy impaired EPCs migratory and tubule-forming capacity in vitro (P < 0.05). Nordy could inhibit in EPCs the functions of proliferation, migration and tubulogenesis induced by VEGF in vitro, which might be a possible mechanism of its anti-EPCs effects.
Antineoplastic Agents ; pharmacology ; Cell Movement ; drug effects ; Cell Proliferation ; drug effects ; Cells, Cultured ; Endothelial Cells ; cytology ; Fetal Blood ; cytology ; Humans ; Masoprocol ; analogs & derivatives ; pharmacology ; Neovascularization, Physiologic ; drug effects ; Stem Cells ; cytology ; Vascular Endothelial Growth Factor A ; antagonists & inhibitors