1.Role of miRNA in the diagnosis, prognosis and treatment of esophageal cancer.
Chinese Journal of Oncology 2011;33(3):161-164
Adenocarcinoma
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diagnosis
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genetics
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metabolism
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therapy
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Animals
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Barrett Esophagus
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metabolism
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Biomarkers, Tumor
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metabolism
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Carcinoma, Squamous Cell
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diagnosis
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genetics
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metabolism
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therapy
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Esophageal Neoplasms
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diagnosis
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genetics
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metabolism
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therapy
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Gene Expression Profiling
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Genetic Therapy
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Humans
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MicroRNAs
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genetics
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metabolism
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Precancerous Conditions
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metabolism
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Prognosis
4.Intravascular lymphomatosis of the prostate gland.
Xiu-rong YIN ; Hong LIU ; Hui-qing CHEN
Chinese Journal of Pathology 2005;34(3):189-190
Aged
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Antigens, CD20
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metabolism
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Humans
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Leukocyte Common Antigens
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metabolism
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Lymphoma, B-Cell
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complications
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metabolism
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pathology
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surgery
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Male
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Prostate
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pathology
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Prostatectomy
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Prostatic Hyperplasia
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complications
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metabolism
;
pathology
;
surgery
6.Type of adenoidal hypertrophy by nasal endoscopic and clinical significance.
Qing-quan ZHANG ; Qiang WANG ; Xiu-mei CHEN
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2010;45(10):859-860
Adenoids
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diagnostic imaging
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pathology
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surgery
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Adolescent
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Child
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Child, Preschool
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Endoscopy
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Female
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Humans
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Hypertrophy
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diagnostic imaging
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pathology
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surgery
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Male
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Nasopharyngeal Diseases
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diagnostic imaging
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pathology
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surgery
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Nose
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surgery
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Radiography
8.Effect of chaperone-mediated autophagy in MPP(+) -induced SH-SY5Y cells and interventional effect of puerarin.
Xun-Cui WANG ; Xiu WANG ; Qing-Lin LI
China Journal of Chinese Materia Medica 2014;39(1):106-112
OBJECTIVETo study the protective effect of puerarin on MPP(+) -induced SH-SY5Y cells by chaperone-mediated autophagy (CMA).
METHODThe Parkinson's disease cell model was established by injuring SH-SY5Y cells with 1 mmol x L(-1) MPP+. The CCK-8 staining was adopted to detect the effect the puerarin of different concentrations on the survival rate of MPP(+)-induced SH-SYSY cells. The autophagosome formation was observed under transmission electron microscope. The AO staining showed the changes in the lysosome activity. RT-PCR was used to detect the changes in Lamp2a and Hsc70 mRNA expressions. The western blotting was adopted to test the expressions of Lamp2a, Hsc70 and alpha-synuclein protein in cells.
RESULTWithin the concentration range of 12. 5-50.0 micromol x L(-1), the pretreatment with puerain for 30 minutes could protect the injury of MPP+ in SH-SY5Y cells, and showed a certain dose-effect relationship. The AO staining and electron microscope showed the effect of puerain within the concentration range of 12.5-50.0 micromol x L(-1) on 1 mmol x L(-1) MPP(+)-induced SH-SY5Y cells; autophagosomes emerged in cells, and increased along with the rise in the puerarin dose. The results of the flow cytometry revealed that 50.0 micromol x L(-1) of puerarin could protect against the increase of the ROS level in 1 mmol x L(-1) MPP(+) -induced SH-SY5Y cells and prevent the oxidative injury. The results of RT-PCR and western blotting indicated that puerain within the concentration range of 12.5-50.0 micromol x L(-1) alleviated the MPP(+)-induced SH-SY5Y cell injury, and inhibited the accumulation of alpha-synuclein proteins in MPP(+) -induced SH-SY5Y cells by up-regulating Hsc70, Lamp2a mRNA and protein level.
CONCLUSIONPuerarin could protect against the MPP(+) -induced cell injury, whose protective mechanism may be related to the chaperone-mediated autophagy pathway of interventional molecules.
Autophagy ; drug effects ; genetics ; HSC70 Heat-Shock Proteins ; genetics ; Humans ; Isoflavones ; pharmacology ; Lysosomal-Associated Membrane Protein 2 ; genetics ; Molecular Chaperones ; genetics ; Parkinson Disease ; drug therapy ; genetics ; Phagosomes ; drug effects ; genetics ; Piperidines ; pharmacology ; Pyrazoles ; pharmacology ; Tumor Cells, Cultured ; Up-Regulation ; drug effects ; genetics
9.Observation of anterior chamber angle change before and after laser iridectomy in primary angle closure glaucoma patients with Stratus optical coherence tomography
Wei-Jia DAI ; Hui-Qing YANG ; Xiu-Yun PAN ;
Ophthalmology in China 1993;0(04):-
Objective To explore the clinical application and outlook of anterior segment observation with Stratus optical coherence tomography(Stratus OCT).Design Prospective case series.Participants 56 eyes in 38 patients with primary angle closure glaucoma (PACG).Methods Morphological changes of anterior chamber angle (ACA) were observed with Stratus OCT before and 4 weeks after laser iridectomy.Opening status of ACA were described and measured with Photoshop software.Main Outcome Measures Opening degree of ACA and ratio of anterior chamber depth to thickness of cornea.Results To a great deal of extent,Stratus OCT could be used to observe the appearance of ACA. Clear images of ACA could be acquired.The ACA before laser was (15.67?5.33) degree and significantly reopened to(26.56?8.17) degree after laser iridectomy (P=0.000).The ratio of anterior chamber depth to thickness of cornea was also changed from (0.39?0.13) pre-treatment to (0.89?0.32) pest-treatment(P=0.000).Conclusions Stratus OCT could be applied to observe the changes of opening degree of ACA easily.The standard of quantization should be consummated in the future.
10.Study on antipyretic action of dipseudoephedrine glycyrrhizin and its effect on heart rate and blood pressure of rats
Ling GAO ; Xue-Ming ZHANG ; Qing YAO ; Xiu-Qin CAO ;
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(11):-
Objective: To study the antipyretic action of Dipseudoephedrine Glycyrrhizin and its effect on heart rate and blood pressure of rats.Methods: The model of pyretic rabbit was established by diphtheria-pertussis-tetanus triple vaccine,and to observe the effect of Dipseudoephedrine Glycyrrhizin on temperature of rabbit. Two-path physiological recorder was used to measure heart rate and blood pressure.Results: The experiment proved that Dipseudoephedrine Glycyrrhizin can decrease the anus temperature of pyretic rabbit obviously.Dipseudoephedrine Glycyrrhizanate had no remarkably effect on heart rate and blood pressure.Conclusion: Dipseudoephedrine Glycyrrhizin has antipyretic action and has no effect on heart rate and blood pressure within studied dose.