AIM: To observe the effect of compound anisodine injection combined with yi-qi-tong-luo acupuncture, hot compress and massage for blepharospasm.
METHODS: Selected in our hospital, 60 cases (78 eyes) with blepharospasm were randomly divided into two groups, 30 cases 39 eyes in each group. The observation group were treated with compound anisodine injection combined with yi - qi - tong - luo acupuncture treatment, and supplemented by hot compress and massage, while the control group only with compound anisodine injection. We compared the treatment effect of the two groups.
RESULTS: In the observation group, 38 eyes were clinically effective, 1 eye was invalid, 35 eyes marked, the total effective rate was 97%, the markedly effective rate was 90%; in control group, 32 eyes were the clinically effective, 7 eyes invalid, 23 eyes marked, the total effective rate was 82%, the markedly effective rate was 59%; the total effective rate and markedly effective rate of observation group were higher than those of the control group with statistical difference (P<0. 05). The recurrence rates of the two groups were 5% and 34%, respectively, and the recurrence rate of the observation group was significantly lower than that of the control group ( P <0. 05).
CONCLUSION: The compound anisodine injection combined with yi - qi - tong - luo acupuncture, hot compress and massage for blepharospasm, can significantly improve the efficiency of treatment, with higher effective rate and lower recurrence rate, compared with using compound anisodine alone.

Objective To explore the clinical significance of detection of IL-17 and peripheral blood eosinophil(EOS) count in whee-zing children under 5 years old.Methods Fifty-three children with recurrent wheezing under 5 years old had been randomly enrolled including 43 boys and 10 girls.They were divided into 2 groups :wheezing group Ⅰ,in which,the patients with atopic high risks(n=27);whee-zing group Ⅱ,in which,the patients without atopic high risks(n=26).Children without infectious diseases such as hernia and renal calculus had been enrolled randomly as control group(n=20),including 11 boys and 9 girls.After taking 3 mL venous blood and centrifuging of patients in 3 groups,the level of IL-17 in the serum was measured by double-antibody sandwich enzyme-linked immunosorbent assay,peripheral blood EOS count was performed in the meantime by means of eosine staining.Results The serum levels of IL-17 in wheezing groups [(1 469.315?978.300) ng?L-1,(263.340?131.800) ng?L-1]were significantly higher than that of control group[(36.478?2.000) ng?L-1](Pa0.05);serum IL-17 and peripheral blood EOS count had positive correlation(r=0.933,P

Men with non-obstructive azoospermia (NOA) can achieve fertility by testicular sperm extraction (TESE) coupled with intracytoplasmic sperm injection (ICSI), the key to which is the successful retrieval of sperm from the testis. Although improved testicular sperm extraction techniques have increased the chances of sperm retrieval, to predict preoperatively the success of sperm retrieval from NOA patients remains challenging. A non-invasive diagnostic technique predicting the presence of sperm in the testis would be useful for avoiding possible surgical intervention. At present, some preoperative variables, such as serum FSH, inhibin B level, testis volume, genetic analysis, histopathology on diagnostic biopsy, Raman Spectroscopy, and molecular and protein markers, have provided new insights into the chances of successful sperm retrieval in NOA males. This review aims to evaluate the preoperative factors currently available for predicting the outcomes of sperm retrieval from NOA patients.
Azoospermia ; therapy ; Biomarkers ; Biopsy ; Genetic Testing ; Humans ; Inhibins ; blood ; Male ; Sperm Injections, Intracytoplasmic ; Sperm Retrieval ; Spermatozoa ; cytology ; Testis ; cytology

AIM: To evaluate the effect of probing of lacrimal passage combining injecting of US-produced TobraDex eye ointment for the treatment of chronic dacryocystitis.METHODS: For 127 cases (129 eyes) of chronic dacryocystitis, first the pyoid secretion gathered in the lacrimal sac was dashed out, and some TobraDex was injected in the middle of the lacrimal passage once per day, repeated for several times. The lacrimal passage was probed when the secretion of lacrimal sac disappeared essentially. The lacrimal passage and immit TobraDex eye ointment was used once every two days, repeated for 3-4 times.RESULTS: In 126 cases (128 eyes) the lacrimal passage was dredged. Only one case (1 eye) the youthful patient did not recover for the opening ectopia of lacrimonasal duct.During the 3mo-1a random visit the chronic dacryocystitis did not recrudesce in the cases of lacrimal passage dredging.CONCLUSION: It is simple and safe to use the probing of lacrimal passage combining injecting of US-produced TobraDex eye ointment to treat the chronic dacryocystitis. This method has good curative effects and can keep the normal physiological structure of lacrimal passage. It does not need any expensive medical equipment and cost less, therefore is advisable for patients.

Objective:To investigate the effects of 125I radioactive particle implantation in the treatment of maxillofacial malignancy. Methods:43 patients with maxillofacial malignancy were treated with 125I radioactive particle implantation.The procedure was carried out according to the treatment planning system(TPS),with the particles spaced uniformly at 1 cm intervals and with the activity of 0.7 m Ciby 41 particles per case on a verage.All patients were followed up for 6-60 months.Results:The whole treatment procedurewas successful,and no particle displaced.The follow-up rate was 93.02% and treatment effective rate(CR+PR) was 90.70%.Norecur-rence was foundinall target areas.The mortality due to tumor was 9.30%and total survival rate was 74.43%.The cumulative survival rate of the patients in 0.5,1,2,3 and 5 years was 93.0%,85.7%,79.3,69.8% and 56.9% respectively.Survival periodon aver-age was 36.06-50.04 months,with the median of 43.05 months.The longest tumor-free survival period was 60 months.Radiation in-jury rate was 20.93% and only level 1 radiation injury was observedinall the cases.Facial nerve dys function was found in 2 cases and recovered after treatment.Conclusion:Treatment of maxillofacial malignancy by implantation of 125I particles is convenient and mini-mally invasive.The treatment canincrease survival rate of the patients and guaran tee the oropharynxes' function.

Objective To evaluate the clinical effect and safety of L-carnitine and coenzyme Q10 on the treatment of chronic heart failure. Methods One hundred ninety-six patients with chronic heart failure cardiacⅢ-Ⅳlevel were paired by age and gender, and each pair were randomly divided into experimental group and the control group, every group has 98 patients.The control group was treated with metoprolol 100 mg, bid +chlorine 50 mg qd +spironolactone 40 mg bid conventional heart failure treatment.The experimental group was added with L-carni-tine (20 mg? kg -1 ,bid) and coenzyme Q10 (10 mg tid) on the basis of conventional treatment. After treatment, the improvement of cardiac function and incidence of adverse drug reactions between the two groups were compared. Results The total effective rate of treatment group (89.80%) is significantly higher than that in control group 63.27%(P<0.05).After treatment, the C reactive protein content and the brain natriuretic peptide ( BNP) level of experimental group were signifi-cantly lower than those in control group ( P<0.05).6 min walking dis-tance of patients in experimental group is longer than that in control group.The left ventricular ejection fraction ( LVEF) of treatment group was significantly higher than that in control group and the left ventricular diastolic diameter ( LVEDD) and left ventricular end systolic diameter ( LVESD) significantly lower than those of control group.The adverse reactions between the two groups have no significant difference (P>0.05).Conclusion L-carnitine combined coenzyme Q10 can significantly improve the treatment effect of severe chronic heart failure without increasing of the incidence of adverse drug reactions.

OBJECTIVESTo study the effectiveness of an artificial liver support system.

METHODSThirty-two patients with medicamentous liver insufficiency were treated with an artificial liver support system in addition to the routine medicinal therapy. Thirty patients treated with routine medicinal therapy only served as controls.

RESULTSThe clinical symptoms (e.g. hepatic encephalopathy) and the laboratory indices (serum total bilirubin and prothrombin time) of the treatment group patients were obviously improved compared with those of the control group patients (P < 0.05). The cure rate and hospitalization days were 90.6% (26/32) and 47 days respectively in the treatment group, and 43.3% (13/30) and 72 days in the control group (P < 0.05).

CONCLUSIONUsing an artificial liver support system combined with routine medicinal therapy is more effective than using medication alone.

Adult ; Aged ; Antineoplastic Agents ; adverse effects ; Antitubercular Agents ; adverse effects ; Female ; Hepatic Insufficiency ; chemically induced ; therapy ; Humans ; Liver, Artificial ; Male ; Middle Aged

Objective To explore the relationship of central obesity and brain-derived neurotrophic factor (BDNF) expression in skeletal muscle.Methods Fifty healthy kunming mice at the neonatal stage were randomly divided into control group (n =20 cases) and test group (n =30 cases).To obtain the obese models,test group mice were injected with 10％ monosodium glutamate (3 mg · g-1 · d-1)for 5 days at cervical subcutaneous.In addition,mice were removed according to the requirements.Finally,we got 12 mice in each group.We detected body weight of the mice every two weeks,then Lee' s index was calculated.Body temperature was detected per week.At the end of 8th week,the blood specimens was collected from the eyeball to detect the serum lipid using automatic biochemistry analyzer.The perirenal white adipose tissue (WAT) and interscapular brown adipose tissue (BAT) were isolated and weighed.Furthermore,the levels of BDNF in skeletal muscle were investigated through western blotting in order to analyze the relationship between BDNF with central obesity.Results At the 6th weekend,the body weight of test and control groups were (39.71±2.55) and (32.83 ±2.30)g,the Lee's indexes were (365.02 ±3.83) and (337.54±4.10) g1/3 · cm-1,at the 8th weekend,the body weight of test and control groups were (48.12 ± 3.61) and (39.51 ± 3.52)g,the Lee's indexes were (361.93 ± 7.12) and (325.17 ± 6.87)g1/3 · cm-1 Above indexes had significant difference between the two groups (P ＜ 0.05,P ＜ 0.01 or P ＜ 0.001).At the 6th weekend,the temperature of the two groups were (36.30 ± 0.07) and (36.67 ± 0.07) ℃,at the 7th weekend,the temperature of the two groups were (36.40 ±0.08)and (36.79 ± 0.10)℃ respectively,at the 8th weekend,the temperature of the two groups were (36.31 ± 0.09)and(36.80 ± 0.10)℃ respectively.Above indexes had significant difference between the two groups.At the 8th weekend,the main indexes in test and control groups were compared:the total cholesterol were(2.91±0.25) and (1.86 ± 0.51) mmol · L-1,the triglyceride were (1.48 ± 0.62) and (0.81 ±0.23)mmol · L-1,the low-density lipoprotein were (0.37 ± 0.06) and (0.29 ± 0.05) mmol · L-1,the WAT were (0.75 ± 0.08) and (0.24 ± 0.05) g,the BAT were (0.31 ± 0.07) and (0.17 ±-0.01) g,the value of the BAT/WAT were (0.41 ±0.08) and (0.71 ± 0.12),the expression of BDNF were (0.63 ± 0.07) and (0.98 ± 0.06).The differences were statistically significant (P ＜ 0.05,P ＜ 0.01 or P ＜ 0.001).Conclusion Down-regulation of BDNF in skeletal muscle may be involved with central obesity of mice by affecting energy metabolism.

Objective To explore the activation and function of Janus protein-tyrosine kinase (JAK)/ signal transducer and activator of transcription (STAT1) signal transduction pathway in kidney,lung and brain of MRL/lpr mice.Methods MRL/lpr mice with systemic lupus erythematosus (SLE) were studied at the age of 12 weeks up.Non-SLE MRL/lpr mice were used as controls.We used phosphospecific antibodies to detect STAT1 activation in kidney,lung and brain by immunohistochemistry and Western blots.Gene expression of the STAT induced feedback inhibitors of cytokine signaling 1 (SOCS-1) was investigated by SYBR green I real-time reverse transcriptase polymerase chain reaction (PCR).Results Phosphorylation of STAT1 protein was markedly activated in these three organs,although renal and pulmonary STAT1 activation were much more evidently activated.SOCS-1 gene expression increased in all three organs,while renal SOCS-1 gene expres- sion increased less than lung and brain.Conclusion The activation of JAK/STATI signal transduction path- way may be pathogenic in the organ involvement and progression of SLE.The pathogenesis of lupus nephritis may also be associated with the down-regulation of SOCS-1 feedback inhibition.

OBJECTIVETo explore the clinical effects of colonic dripping with Taihuang liquid (THL) in treating neonatal hyperbilirubinemia (HBE).

METHODSOne hundred and thirty-eight neonates with HBE were randomly assigned to two groups. Conventional treatment and nursing were given to both groups, and THL was given additionally to the observation group by colonic dripping.

RESULTSSignificant differences between the observation group and the control group were shown in frequency of defecation (4.6 +/- 1.3 times/d vs 2.0 +/- 1.1 times/d), daily serum bilirubin reduction (31.5 +/- 10.1 micromol/L vs 23.3 +/- 8.3 micromol/L), and days for normalizing serum bilirubin level (5.6 +/- 3.5 d vs 7.8 +/- 4.1 d, all P < 0.01).

CONCLUSIONColonic dripping of THL could promote the excretion of bilirubin, so as to decrease the level of serum bilirubin in neonates with HBE.

Bilirubin ; blood ; Drugs, Chinese Herbal ; administration & dosage ; Female ; Humans ; Hyperbilirubinemia, Neonatal ; blood ; drug therapy ; Infant ; Infant, Newborn ; Male