1.Effects of fluoride on the expression of c-fos in mouse fibroblast and osteoblast
Ling, QI ; Xiu-yun, ZHANG ; Hui, XU ; Ling, JING
Chinese Journal of Endemiology 2009;28(2):130-133
Objective To observe the expression of c-fos mRNA and protein in fluoride treated mouse fibroblast (FB) and osteoblast (OB) and to further explore the effects of c-fos in the osteogenic action of FB. Methods Mouse FB and OB were divided into control group and six fluoride groups (0, 0.0001, 0.0010, 0.1000, 1.0000, 10.0000,20.0000 mg/L F-), and the levels of c-fos protein at 2,4,24,48,72 h and c-fos mRNA at 48 h were measured by using ELISA and RT-PCR methods. Results Compared with the control group, fluoride increased the content of c-fos protein obviously in all FB group(P<0.01); and it is increased in 0.0001,0.0010 mg/L groups at 48 h (0.73±0.04, 0.64±0.14) and 0.0001 mg/L group at 72 h(0.70±0.17) in OB compared with the control group (0.32±0.04,0.27±0.05, P<0.01). Compared with the control group (0.95±0.11), RT-PCR revealed an increasing tendency of the expression of c-fos mRNA at 48 h in FB (1.06±0.16, 1.06±0.12,1.12±0.16,1.04±0.15,1.04±0.10,1.15±0.29), but the difference was not statistically significant(P>0.05); however, a statistically significant difference(P<0.01) of c-fos mRNA in 20.0000 mg/L group(1.40±0.17) in O B was found compared with the control group (1.06±0.06). Conclusion The higher expression of c-fos mRNA and protein in FB induced by fluoride may play an important role in the transformation of osteoblastic phenotype as well as increase the osteogenesis ability in FB.
2.Correlation between myocardial injury induced by acute carbon monoxide poisoning and NT proBNP.
Xiu-ling TAN ; Xu-gong REN ; Yu-guo CHEN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2010;28(6):454-455
Adult
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Aged
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Carbon Monoxide Poisoning
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blood
;
pathology
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Creatine Kinase
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blood
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Female
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Humans
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Male
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Middle Aged
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Myocardium
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pathology
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Natriuretic Peptide, Brain
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blood
;
Peptide Fragments
;
blood
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Troponin I
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blood
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Young Adult
3.Progress on treatment of transverse patella fractures with tension band fixation.
Xiu-ling YU ; Chao XU ; Shun-dong LI ; Jian-dong ZHAN ; Zai-qiang XU
China Journal of Orthopaedics and Traumatology 2015;28(11):1069-1074
Transverse fracture is the most common in patella fracture and tension band fixation is one of the most effective methods. Surgical wire tension band technique is simple, the use of materials is also simple, but it is not strong and difficult to promote. Kirschner tension band technique can get satisfactory reduction with reliable fixation, but it is easy to complicate with steel wire breakage and Kirschner loosening. Screw tension band technique inherits the traditional advantages of simple manipulation and reliable fixation, also overcomes the disadvantages of early activity limitations caused soft tissue irritation of tension band around knee, the slippage and breakage of internal fixation, and the technique can be popularized generally.
Bone Wires
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Fracture Fixation, Internal
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methods
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Humans
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Patella
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injuries
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surgery
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Stress, Mechanical
5.Effects of pH9.0 Tris-EDTA and pH6.0 citrate on antigen retrieval.
Xin-Lan LUO ; Yan-Hui LIU ; Heng-Guo ZHUANG ; Xiu-Ling CAI ; Jie XU
Chinese Journal of Pathology 2005;34(11):749-751
Antigens
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analysis
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Cervical Intraepithelial Neoplasia
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metabolism
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Citric Acid
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Cyclin-Dependent Kinase Inhibitor p21
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analysis
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Edetic Acid
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Formaldehyde
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Hot Temperature
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Humans
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Hydrogen-Ion Concentration
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Immunohistochemistry
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Intestinal Mucosa
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immunology
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Ki-67 Antigen
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analysis
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Microwaves
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Palatine Tonsil
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metabolism
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Proto-Oncogene Proteins c-bcl-6
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analysis
6.Maple syrup urine disease in a neonate.
Ya LING ; Yan QIAN ; Xiu-Lan PENG ; Kai WANG ; Jie-Jin GAO ; Ai-Qin XU
Chinese Journal of Contemporary Pediatrics 2009;11(11):945-946
7.Early Diagnosis of Myocardial Injury in Neonatal Asphyxia
ling-min, XU ; zhan, ZHANG ; li-ting, JIA ; bin, LUAN ; xiu-yong, CHENG
Journal of Applied Clinical Pediatrics 2006;0(22):-
Objective To investigate the clinical value of cardiac troponin Ⅰ(cTnⅠ) and creatinine kinase MB(CK-MB) in early diagnosis of myocardial injury(MCI) in neonatal asphyxia.Methods The serum cTnⅠ and CK-MB in neonates [34 with asphyxia and MCI,38 with asphyxia but no MCI(NMCI)],and 30 cases of normal control(NC) were measured with direct immunoassay chemiluminometric technology and immunoinhibition enzymes-activated assay.Results The cTnⅠ level in NC group had no changes within 10 days after birth,MCI group were significantly higher than those in NMCI and NC groups(all P0.05).The diagnostic sensitivity,specificity and accuracy of cTnⅠ for neonates with MCI were 91%,88% and 89%,respectively;and of CK-MB were 85%,68% and 74%,respectively.Conclusions cTnⅠ and CK-MB can be taken as early diagnostic markers of MCI in neonates with asphyxia,(cTnⅠ) is better than CK-MB.
8.Efficacy of intravitreal ranibizumab injection combined with macular grid photocoagulation for diabetic macular edema
Hu-Lin, JIANG ; Xu-Wei, HAN ; Sheng-Qi, ZHANG ; Xiu-Ling, FANG ; Bo-Jun, ZHAO
International Eye Science 2014;(7):1253-1256
AlM:To evaluate the clinical efficacy of intravitreal injection of ranibizumab combined with macular grid photocoagulation for diabetic macular edema ( DME) .METHODS:Totally 60 eyes ( 60 patients ) with DME were randomly divided into 2 groups: 30 eyes of simple injection group underwent intravitreal injection of ranibizumab, and 30 eyes of combined treatment group underwent intravitreal injection of ranibizumab and macular grid photocoagulation 1wk later. The best corrected visual acuity ( BCVA ) , central macular thickness ( CMT ) measured by optical coherence tomography ( OCT ) and postoperative complications were observed.
RESULTS:ln simple injection group, the BCVA after operation were separately 0. 390 ± 0. 075 (4wk), 0. 367 ± 0. 088 (8wk) and 0. 319 ± 0. 064 (12wk), the CMT after operation were separately 221. 63 ± 112. 34μm (4wk), 337. 73±99. 56μm (8wk) and 432. 92 ± 100. 46μm (12wk), which were much better than pre-operation. But during follow-up, the BCVA presented down trend and the CMT was on the rise slowly. ln combined treatment group, the BCVA after operation were separately 0. 385 ± 0. 036 (4wk), 0.382±0.079 (8wk) and 0.377±0.097 (12wk),the CMT after operation were separately 249. 77 ± 106. 55μm (4wk), 270. 40 ± 92. 88μm (8wk) and 275. 84 ± 97. 34μm (12wk ), which were satisfactory and steady during follow-up, better than simple injection group (P<0. 05).CONCLUSlON:lntravitreal injection of ranibizumab can effectively improve visual acuity and decrease central foveal thickness for patients with DME, combining with macular grid photocoagulation can ensure therapeutic effects steady and permanent.
9.Effects of low calcium and high fluoride on expressions of receptor activator of nuclear factor-kappa B ligang mRNA in spleens of rats
Chun-hong, WANG ; Xiu-li, WANG ; Ai-ping, LU ; Hui, XU ; Guang-sheng, LI ; Ling, JING
Chinese Journal of Endemiology 2010;29(2):127-129
Objective To study the different expressions of receptor activator of nuclear factor-kappa B ligang(RANKL) mRNA in spleens of rats fed with diet of low calcium and high fluoride. Methods A 2× 2×2 factorial design was used and the factors were calcium, fluoride and action time. In the design, 40 Wistar rats [average body mass(118.9±13.5)g] were divided into four groups randomly by weight: control with normal diet (0.790%, calcium), low calcium group with low calcium intake(0.063%, calcium), high fluoride group with normal diet and high fluoride intake(100 mg/L, fluoride) and low calcium and high fluoride group with low calcium and high fluoride intake. After 4 and 8 months, 5 rats of each group were sacrificed and total RNA was extracted from spleen. And the expression levels of RANKL mRNA were determined by reverse transcription polymerase chain reaction (RT-PCR). Results At time of 4 months, the expression level of RANKL mRNA was 0.13± 0.05,0.13± 0.03,0.17±0.02,0.27± 0.05 and at time of 8 months, it was 0.11 ± 0.01,0.16 ± 0.02,0.16± 0.03,0.36 ± 0.07 in control group, low calcium group, high fluoride group, low calcium with high fluoride group, repectively. The factorial design AVONA showed that low calcium and high fluoride had significant effects on RANKL mRNA expression(F = 40.224,56.679, all P < 0.05) while action time had not(F = 2.850, P > 0.05 ). The interactions of low calcium with high fluoride or high fluoride with action time were signifieant(F = 7.247, 18.789, all P < 0.05) while the interaction of high fluoride with action time was not(F = 1.751, P > 0.05). Conclusions Low calcium alone or high fluoride alone or low calcium with high fluoride or low calcium with action time can increase the the RANKL mRNA expression level. High fluoride does not affect the RANKL mRNA level as the action time is prolonged.
10.The efficacy of 125I radio active particle implantation in the treatment of maxillofacial malignancy of 43 cases
Xiu YAO ; Ling GU ; Ying LIU ; Shuangshuang LI ; Dong WANG ; Lu YANG ; Lihong XU ; Chongjian FU
Journal of Practical Stomatology 2016;32(2):220-224
Objective:To investigate the effects of 125I radioactive particle implantation in the treatment of maxillofacial malignancy. Methods:43 patients with maxillofacial malignancy were treated with 125I radioactive particle implantation.The procedure was carried out according to the treatment planning system(TPS),with the particles spaced uniformly at 1 cm intervals and with the activity of 0.7 m Ciby 41 particles per case on a verage.All patients were followed up for 6-60 months.Results:The whole treatment procedurewas successful,and no particle displaced.The follow-up rate was 93.02% and treatment effective rate(CR+PR) was 90.70%.Norecur-rence was foundinall target areas.The mortality due to tumor was 9.30%and total survival rate was 74.43%.The cumulative survival rate of the patients in 0.5,1,2,3 and 5 years was 93.0%,85.7%,79.3,69.8% and 56.9% respectively.Survival periodon aver-age was 36.06-50.04 months,with the median of 43.05 months.The longest tumor-free survival period was 60 months.Radiation in-jury rate was 20.93% and only level 1 radiation injury was observedinall the cases.Facial nerve dys function was found in 2 cases and recovered after treatment.Conclusion:Treatment of maxillofacial malignancy by implantation of 125I particles is convenient and mini-mally invasive.The treatment canincrease survival rate of the patients and guaran tee the oropharynxes' function.