Gastrectomy ; Humans ; MicroRNAs ; analysis ; Statistics as Topic ; Stomach Neoplasms ; genetics ; metabolism

Animals ; Blood Gas Analysis ; Brain Ischemia ; complications ; metabolism ; physiopathology ; Lung Injury ; etiology ; metabolism ; physiopathology ; Male ; Pyrazines ; pharmacology ; Rats ; Rats, Wistar ; Reperfusion Injury ; complications ; metabolism ; physiopathology

Objective To explore the cardiovascular risk factors and clinical features of coronary lesions in male patients with premature coronary artery disease(CAD).Methods A total of 448 male patients with CAD confirmed by angiography were divided into two groups by age: premature CAD(n=145,

Antioxidants ; metabolism ; pharmacology ; Down-Regulation ; Gene Expression Regulation ; Humans ; NF-E2-Related Factor 2 ; genetics ; metabolism ; physiology ; Neoplasms ; genetics ; metabolism ; Oxidative Stress ; genetics ; physiology ; Response Elements ; physiology ; Signal Transduction ; physiology

Humans ; Job Satisfaction ; Occupational Health ; Reward ; Stress, Psychological ; physiopathology ; Surveys and Questionnaires ; Workload

This study was to improve the way for selecting ura5 mutants of Cryptoccocus neoformans Cap59 capsule-deficient strains.They were induced by Diethyl Sulfate. Ura5 mutants were screened by 5-fluoroorotic acid counter selection method. Using the new method, we obtained two ura5 mutants of Cryptoccocus neoformans Cap59 capsule-deficient strain.A easy method that was used to screen ura5 mutants of Cryptoccocus neoformans has been established.

Objective:To explore the influence of low-iodine diet on the expression of homeobox gene NKX-2.2 in rat cerebrum tissue,and to explain the possible molecular mechanism of cerebrum development retardation caused by low-iodine.Methods:Female Wistar rats were randomly divided into two groups:Low-iodine group and control group,both fed with low-iodine feed,given the deionized water and KIO3 solution respectively,they were drawn from the 16-day pregnancy,new-born and 20th days old low-iodine and normal age offspring after three months,and detect the content of NKX-2.2 mRNA in the cerebrum tissue by Real-time fluorescence quantitative PCR techniques.Results:The thyroid hormone levels of low-iodine group in serum were significantly lower than the control group(P

Objective To evaluate 99tcm-DTPA nuclear dynamic imaging in distinguishing the renal occupied disease.Methods A total of 164 in-patients with renal occupied disease who underwent surgery were included.According to the pathological diagnosis,119 patients had malignant tumors,and 45 patients had benign diseases.All patients’ imaging was retrospectively analyzed.Application of 99Tcm-DTPA nuclear dynamic imaging in renal occupied disease was compared with ultrasonography (US),computed tomography (CT),magnetic resonance imaging (MRI),intravenous pyelogram (IVP),and positron emission tomography (PET)-CT.Results The accuracy rates of different imaging methods in distinguishing between renal malignant and benign disease were 99Tcm-DTPA (84 ％,45 ％),US (72 ％,64 ％),CT ( 91％,92 ％),MRI (50 ％,67 ％),IVP (50 ％, 17 ％), respectively.The diagnostic accuracy rate of PET-CT for malignant tumors was 67 ％.The accuracy rates of 99Tcm-DTPA in distinguishing different phases of renal cell carcinoma were statistically significant (x 2 =83.4, P ＜ 0.01), while the accuracy rates in distinguishing renal cyst from renal angiomyolipoma were not statistically different.With the greater diameter, the diagnostic accordance rate is higher (x 2 =16.05,P ＜ 0.05).Conclusion 99Tcm-DTPA could be used not only to evaluate the renal function quantificationally,but also be helpful to distinguish renal malignant tumor from benign disease.

Objective To investigate the anti-proliferation and anti-migration dual effects of aloeemodin on KB cells and its mechanisms.Methods KB cells were treated with 2.5,5,10,20 and 40 μmol/L aloe-emodin.Crystal violet assay was used to determine the long-term growth inhibition of aloeemodin on human oral cancer KB cells.Scratch wound-healing motility assay was used to measure the antimigration effect.The protein kinase Cα and c-myc expression changes in protein levels were detected by Western blotting.Results A durable cell growth inhibitory effect of aloe-emodin on KB cells was found.Treatment of aloe-emodin resulted in the inhibition of cell migration.The protein kinase Cα and c-myc in protein levels were decreased upon treatment with aloe-emodin compared with controls.Condusions The anti-proliferation and anti-migration effects of aloe-emodin on KB cells are associated with the suppression of protein kinase C pathway.

Objective To investigate the effects of microRNA(miRNA)on proliferation of cultured human squamous cell carcinoma of tongue Tca8113 cells.Methods The mimics or inhibitors of miRNA-31 or miRNA-139 were transfected into Tca8113 cells using liposome.Tca8113 cell proliferation was detected by 3-(4,5-dimethyhhiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay.Results The absorbance (A) values of control group at 24 h,48 h and 72 h were 0.125±0.002,0.169±0.002 and 0.216±0.004.respectively.The mimics of miRNA-31 increased Tca8113 cell proliferation.with A values inereasing to 0.136±0.001(P<0.001).0.186±0.004(P<0.001)and 0.249±0.012(P<0.01),respectively.The inhibitors of miRNA-139 also inereased A values to 0.148±0.002(P<0.001).0.214±0.002(P<0.001)and 0.250±0.009(P<0.01),respectively.Contrast with these results,the inhibitors of miRNA-31 decreased Tca8113 cell proliferation,with A values decreasing to 0.145 4-0.001 and 0.155±0.011 (both of P<0.001) at 48 h and 72 h,respectively.The mimics of miRNA-139 also decreased A to 0.135±0.001 and 0.170±0.009 (both of P<0.001).Conclusions miRNA-31 and miRNA-139 play an important role in the carcinogenesis of human tongue carcinomas.It may become a new method for the treatment of tongue carcinomas by adjustment the activities of miRNA.