1.The role of heat shock protein 70 in regulating neuroinflammation.
Wen-wen YU ; Xiu-qi BAO ; Hua SUN ; Dan ZHANG
Acta Pharmaceutica Sinica 2015;50(8):945-950
Neurodegenerative disease is characterized by progressive loss of neurons in specific brain regions that results in neuronal dysfunction of the central nervous system. Although the pathological mechanism is not fully established, the activation of glial cells mediated neuroinflammation appears to be involved. Heat shock protein 70 (HSP70) is originally described as intracellular chaperone, which plays an important role in protein quality control in cells. However, recent study showed that up-regulation of HSP70 had anti-inflammatory effects in the brain. HSP70 protected neurons from damage and improved neurological function by decreasing inflammatory response as indicated by inactivation of glial cells and inhibition of pro-inflammatory cytokine release. So it is of great significance to find new compounds targeting at HSP70 as neuroprotective agents to delay the progress of neurodegenerative disease. This review will focus on the role of HSP70 in neuroinflammation and the recent advances in using HSP70 as a target for the treatment of neurodegenerative disease.
Brain
;
physiopathology
;
Cytokines
;
HSP70 Heat-Shock Proteins
;
physiology
;
Humans
;
Inflammation
;
pathology
;
Neurodegenerative Diseases
;
physiopathology
;
Neurons
;
pathology
;
Neuroprotection
;
Up-Regulation
2.Research progress in the study of protective effect of tanshinone IIA on cerebral ischemic stroke.
De-chuan LI ; Xiu-qi BAO ; Hua SUN ; Dan ZHANG
Acta Pharmaceutica Sinica 2015;50(6):635-639
Danshen is one of the traditional Chinese herbal medicines and nas a long history or being used clinically in the treatment of cardiovascular and cerebrovascular conditions such as coronary heart disease and angina pectoris. Tanshinone IIA is a derivative of phenanthrene-quinone isolated from Danshen. It has been reported to be the major bioactive compound of Danshen and has diverse biological effects. Recent studies demonstrated that tanshinone IIA had neuroprotective effects on experimental ischemic stroke through its antiinflammatory, anti-oxidant, anti-apoptosis effects and its inhibitory effect on excitatory amino acid toxicity. In this review, we summarized all the recent progresses on the protective effect of tanshinone IIA on cerebral ischemic stroke. Hopefully, this article will throw some light on further study and application of tanshinone IIA.
Antioxidants
;
therapeutic use
;
Apoptosis
;
Diterpenes, Abietane
;
therapeutic use
;
Drugs, Chinese Herbal
;
therapeutic use
;
Humans
;
Salvia miltiorrhiza
;
chemistry
;
Stroke
;
drug therapy
3.Chymothorax in a newborn infant.
Wei-dong SU ; Xiu-rui DENG ; Man-hua BAO
Chinese Journal of Pediatrics 2003;41(12):933-933
4.Recent advances on study of human breast stem cells.
Ding-bao CHEN ; Dan-hua SHEN ; Xiu KAN
Chinese Journal of Pathology 2007;36(6):423-425
Actins
;
metabolism
;
Breast
;
cytology
;
metabolism
;
Breast Neoplasms
;
metabolism
;
pathology
;
Cell Differentiation
;
Female
;
Humans
;
Keratins
;
metabolism
;
Neoplastic Stem Cells
;
metabolism
;
pathology
;
Signal Transduction
;
Stem Cells
;
cytology
;
metabolism
5.Recent advances in study of sphingolipids on liver diseases.
Shao-yuan WANG ; Jin-lan ZHANG ; Dan ZHANG ; Xiu-qi BAO ; Hua SUN
Acta Pharmaceutica Sinica 2015;50(12):1551-1558
Sphingolipids, especially ceramide and S1P, are structural components of biological membranes and bioactive molecules which participate in diverse cellular activities such as cell division, differentiation, gene expression and apoptosis. Emerging evidence demonstrates the role of sphingolipids in hepatocellular death, which contributes to the progression of several liver diseases including ischaemia-reperfusion liver injury, steatohepatitis or hepatocarcinogenesis. Furthermore, some data indicate that the accumulation of some sphingolipids contributes to the hepatic dysfunctions. Hence, understanding of sphingolipid may open up a novel therapeutic avenue to liver diseases. This review focuses on the progress in the sphingolipid metabolic pathway with a focus on hepatic diseases and drugs targeting the sphingolipid pathway.
Apoptosis
;
Ceramides
;
metabolism
;
Fatty Liver
;
metabolism
;
physiopathology
;
Humans
;
Liver Diseases
;
metabolism
;
physiopathology
;
Lysophospholipids
;
metabolism
;
Reperfusion Injury
;
metabolism
;
physiopathology
;
Sphingolipids
;
metabolism
;
Sphingosine
;
analogs & derivatives
;
metabolism
7.Studies on chemical constituents of Acroptilon repens.
Dong-bao ZHAO ; Wei ZHANG ; Ming-jing LI ; Xiu-hua LIU
China Journal of Chinese Materia Medica 2006;31(22):1869-1872
OBJECTIVETo investigate the chemical constituents of Acroptilon repens.
METHODThe ethanol extract of A. repens was isolated and purified by means of chromatography. These compounds were identified by their spectral data.
RESULT11 compounds were isolated and identified as 2alpha, 9beta-dihydroxy-dehydrocostus lactone (1) , cynaropicrin (2) , apigenin (3) , stigmasterol (4) , 4' -hydroxywogonin (5) , ethyl caffeate (6) , p-methoxy-cinnamic acid (7) , luteolin (8) , daucosterol (9) , apigenin-7-O-beta-D-glucoside (10) , syringin (11).
CONCLUSIONCompounds 5-11 were isolated from A. repens for the first time. Compound 1 is new compounds.
Apigenin ; chemistry ; isolation & purification ; Asteraceae ; chemistry ; Caffeic Acids ; chemistry ; isolation & purification ; Flavanones ; chemistry ; isolation & purification ; Lactones ; chemistry ; isolation & purification ; Molecular Structure ; Plant Extracts ; chemistry ; isolation & purification ; Plants, Medicinal ; chemistry ; Sesquiterpenes ; chemistry ; isolation & purification
8.Treatment of ovarian cyst after ovulation-induction with sanjie zhentong capsule.
Hai-Yan WANG ; Xiu-Hua BAO ; Jun-Feng DAI
Chinese Journal of Integrated Traditional and Western Medicine 2008;28(11):1026-1028
OBJECTIVETo observe the efficacy of Sanjie Zhentong Capsule (SJC) in treating ovarian cyst after ovulation-induction.
METHODSFifty-eight patients with ovarian cyst were randomly assigned to two groups, 33 in the treated group treated with SJC and 25 in the control group treated with temporization for 1 month. Changes of estradiol (E2), luteinizing hormone (LH) and follicle stimulating hormone (FSH) level as well as condition of cyst before and after treatment were observed and compared. And the time for complete disappearance (TCD) of cyst and the pregnant rate within 4 months were followed-up.
RESULTSEffective rate (no fluid cyst sized over 1.0 cm could be found in bilateral ovary and E2 <280 pmol/L) in the treated group was 81.8% (27/33) and 52.0% (13/25) in the control group, showing significant difference between them (P <0.05). Level of E2 decreased in both groups, but the lowering was more significant in the treated group, after 1 month, it being 220.54 +/- 96.23 pmol/L vs 372.56 +/- 330.62 pmol/L (P <0.05). The changes of LH and FSH levels were of no statistical significance (P >0.05). TCD in the treated group was 1.18 +/- 0.46 months, which was shorter than that in the control group (1.96 +/- 1.34 months, P <0.05). The pregnant rate within 4 months in the two groups was 60.6% (20/ 33) and 32.0% (8/25) respectively, showing significant difference (P < 0.05).
CONCLUSIONSJC has good efficacy in treating ovarian cyst after ovulation-induction.
Adult ; Capsules ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Follicle Stimulating Hormone ; blood ; Humans ; Luteinizing Hormone ; blood ; Ovarian Cysts ; blood ; drug therapy ; therapy ; Ovulation Induction
9.Rapid detection of novel avian influenza virus subtype H7N9 by multiplex real-time RT-PCR.
Bao-Zheng LUO ; Qiu-Hua MO ; Ru-Shu LI ; Qing-Ru BO ; Hai-Nie XU ; Cai-Hua SHA ; Xiu-Yun LIAO
Chinese Journal of Virology 2014;30(1):1-5
In order to develop a rapid detection kit for novel avian influenza virus (AIV) subtype H7N9, two sets of specific primers and probes were designed based on the nucleotide sequences of hemagglutinin antigen (HA) and neuraminidase antigen (NA) of novel H7N9 virus (2013) available in GenBank to establish the method of TaqMan probe-based multiplex real-time RT-PCR for rapid detection of AIV subtype H7N9. The primer and probe of HA were for all H7 subtype AIVs, while the primer and probe of NA were only for novel N9 subtype AIVs. The results showed that this method had high sensitivity and specificity. This method was applicable to the testing of positive standard sample with a minimum concentration of 10 copies/microL; it not only distinguished H7 subtype from H1, H3, H5, H6, and H9 subtypes, but also distinguished novel N9 subtype from traditional N9 subtype. A total of 2700 samples from Zhuhai, China were tested by this method, and the results were as expected. For the advantages of sensitivity and specificity, the method holds promise for wide application.
Animals
;
Birds
;
virology
;
Influenza A Virus, H7N9 Subtype
;
genetics
;
isolation & purification
;
physiology
;
Influenza in Birds
;
prevention & control
;
virology
;
Real-Time Polymerase Chain Reaction
;
methods
;
Species Specificity
;
Taq Polymerase
;
metabolism
;
Time Factors
10.Study on content variation of triptolide in medicinal material of Tripterygium.
Jin-Ping SI ; Wen-Hua HUANG ; Bao-Lin GUO ; Xiu-Chun RUAN ; Shi-Jie LI
China Journal of Chinese Materia Medica 2006;31(24):2026-2030
OBJECTIVETo study the content variation of triptolide in medicinal material of Tripterygium and provide theoretical basis for the hereditary improvement, the gathering and process, the quality evaluation and the provenance division in medicinal material of Tripterygium.
METHODHPLC method was used to determine the content of triptolide.
RESULTThe relations between triptolide and germplasm, growth year, gathering season were found out basically.
CONCLUSIONThe triptolide contents in xylem are affected by hereditary factors remarkably. While the triptolide contents in phloem are not affected obviously. The accumulation of triptolide needs the certain growth years. However when growth is beyond certain years, the triptolide content decreases with the disintegration of secondary metabolism in xylem. The triptolide in xylem is highest in winter and decreasing in growing season. The triptolide in phloem is less affected by the season.
Chromatography, High Pressure Liquid ; Diterpenes ; analysis ; metabolism ; Ecosystem ; Epoxy Compounds ; analysis ; metabolism ; Phenanthrenes ; analysis ; metabolism ; Plant Roots ; anatomy & histology ; chemistry ; Plants, Medicinal ; anatomy & histology ; chemistry ; growth & development ; Seasons ; Species Specificity ; Time Factors ; Tripterygium ; anatomy & histology ; chemistry ; growth & development ; Xylem ; chemistry