3.Interferon Stimulating Effect of Ciwujia Eleutherosides or Polysaccharide in S801 Leukemic Cell Cultures
Ji-Cheng YANG ; Hong-Zhen XIU ; Jing-Shan LIU ; Pei-Jun XIU
Chinese Journal of Immunology 1986;0(04):-
S801 leukemic cell line established by our department was used as cells for interferon (IFN) induction and stimulating induction. S801 cell culture of concentration of 5-8x10~6 cells/ml was used for IFN induction and IFN titer of 3.55 Lg IU/ml was obtained by classical procedure. The IFN titer obtained S801 cell cultures after pretreatment with Ciwujia eleutherosides B. D and E or polysaccharide (PES) combined with priming procedure,then using Sendai Virus as an inducer was 10-20 times higher than that in cultures without any pretreatment. These Chinese medical herbs may be the ideal IFN helpinducers. This cell line may be applied for IFN production.
4.Effects of High Intensity Focused Ultrasound on AFP mRNA in Peripheral Blood of Patients with Hepatocellular Carcinoma
Fuyu LI ; Hong LIU ; Quanyi LONG ; Lisheng JIANG ; Ruihua XIU ;
Chinese Journal of Bases and Clinics in General Surgery 2003;0(03):-
Objective To evaluate the effects of high intensity focused ultrasound (HIFU) on hepatic cancer cells spreading in blood.Methods AFP mRNA in peripheral blood of 19 patients with hepatocellular carcinoma was detected before and after HIFU therapy by RT PCR.Results ①Before HIFU therapy, 11 of 19 cases were AFP mRNA positive (57.9%), while the control group were all negative. AFP mRNA was correlated with some clinical parameters such as serum AFP level, tumor size, portal vein embolism and extrahepatic metastasis ( P 0.05). ③After one week of HIFU therapy, the AFP mRNA positive rate of the group with tumor size less than 8 cm was much lower than that of tumor size larger than 8 cm ( P
5.Survey on Sleep Time in Korean Children
xiu-fang, HAO ; yan-xiang, MA ; hong-hua, LIU
Journal of Applied Clinical Pediatrics 2006;0(22):-
Objective To evaluate the sleep characteristics of the Korean children,and explore the measurement of improving the sleep qua-lity of minority nationality children.Methods The sleep time of 1 183 from 3 to 12 years age Korean children were investigated with questionnaire The sleep time of korean children in different ages and cities were compared.Results The mean time of total sleep time in Korean children was(10.06?1.29) h,which was decreasing with the age′s increasing.The difference in different ages groups was remarkable(Pa
6.Analysis on the plasma level of alpha2 -antiplasmin and Arg6Trp C/T gene polymorphism in 102 venous thromboembolism patients.
Li-hong HOU ; Ling ZHANG ; Xiu-e LIU
Chinese Journal of Hematology 2012;33(7):581-583
Adolescent
;
Adult
;
Aged
;
Aged, 80 and over
;
Apolipoproteins E
;
blood
;
genetics
;
Case-Control Studies
;
Female
;
Humans
;
Male
;
Middle Aged
;
Polymorphism, Genetic
;
Venous Thromboembolism
;
blood
;
genetics
;
Young Adult
;
alpha-2-Antiplasmin
;
analysis
;
genetics
8.Intravascular lymphomatosis of the prostate gland.
Xiu-rong YIN ; Hong LIU ; Hui-qing CHEN
Chinese Journal of Pathology 2005;34(3):189-190
Aged
;
Antigens, CD20
;
metabolism
;
Humans
;
Leukocyte Common Antigens
;
metabolism
;
Lymphoma, B-Cell
;
complications
;
metabolism
;
pathology
;
surgery
;
Male
;
Prostate
;
pathology
;
Prostatectomy
;
Prostatic Hyperplasia
;
complications
;
metabolism
;
pathology
;
surgery
10.Construction of Recombinant Yeast Converting Xylose Angd Glucose to Ethanol
Zhen-Hong YUAN ; Ya-Ping PAN ; Ji-Kai LIU ; Yong-Jie YAN ; Xiu-Shan YANG ;
Microbiology 1992;0(03):-
Candida shehatae xyl1 gene and Pichia stipitis xyl2 gene were amplified by PCR and the xyl1 and xyl2 were both placed under the promoter GAL of vector pYES2 to produce the recombinant expression vector pYES2-P12. Subsequently the pYES2-P12 vector was transformed into S. cerevisiae YS58 by LiAc to produce the recombinant yeast YSS8-12. It was indicate that the recombinant yeast YSS8-12 could converse xylose to ethanol with the xylose consumption rate of 81. 3%.