Biomedical Research ; China ; Preventive Medicine ; Research Support as Topic

Biomedical Research ; China ; Preventive Medicine ; Research Support as Topic

OBJECTIVETo analyze the projects of the general program in preventive medicine submitted to and funded by National Natural Science Foundation of China (NSFC) from 2004 to 2006, and to discuss the disadvantages of these projects and foundation trends.

METHODS4124 projects submitted and 656 projects supported in 16 fields of preventive medicine between 2004 to 2006 were analyzed statistically.

RESULTS1082, 1378 and 1664 projects were submitted to NSFC and 199, 210 and 247 projects were financially supported in last three years, respectively, with selecting rate 18.4%, 15.2% and 14.8%, respectively. The number and the selection rate of projects supported in the different study fields from 2004 to 2006 were 15 (7.5%), 24 (11.4%) and 18 (7.3%) in environmental health, 8 (4.0%), 12 (5.7%) and 15 (6.1%) in occupational health, 30 (15.1%), 24 (11.4%) and 31 (12.6%) in nutrition and food hygiene, 3 (1.5%), 4 (1.9%) and 4 (1.6%) in child and adolescent health, 14 (7.0%), 20 (9.5%) and 20 (8.1%) in toxicology, 7 (3.5%), 7 (3.3%) and 2 (0.8%) in social medicine, 17 (8.5%), 22 (10.5%) and 31 (12.6%) in epidemiology, 7 (3.5%), 7 (3.3%) and 10 (4.0%) in endemiology, 2 (1.0%), 5 (2.4%) and 5 (2.0%) in biostatistics, 0 (0%), 2 (1.0%) and 1 (0.4%) in health laboratory technology, 7 (3.5%), 2 (1.0%) and 7 (2.8%) in vector biology, 15 (7.5%), 9 (4.3%) and 16 (6.5%) in parasitology, 0 (0%), 2 (1.0%) and 0 (0%) in disinfection, 58 (29.1%), 51 (24.3%) and 58 (23.5%) in infectious disease, 3 (1.5%), 1 (0.5%) and 5 (2.0%) in sexually transmitted disease, 13 (6.5%), 18 (8.6%) and 24 (9.7%) in dermatology, respectively. The contents of these research subjects reflected that more importance has been attached to the fields of preventive medicine. However, it is necessary that original and innovatory research should be further strengthened, and the field and prospective study should be emphasized in the future.

CONCLUSIONRapid advancement have been made in the fields of toxicology, epidemiology, environmental health, nutrition and food hygiene, and infectious disease, while other areas such as social medicine, health laboratory technology, disinfection and sexually transmitted disease need a rapid advancement. In general, the levels of the projects received and funded have been elevating yearly.

China ; Financial Support ; Fund Raising ; Preventive Medicine ; economics ; statistics & numerical data ; Research Support as Topic ; statistics & numerical data

OBJECTIVE: To investigate the effect of Bmi-1 expression on the chemosensitivity of THP-1 cells and its relative mechanism. METHODS: The pGenesil-2-Bmi-1 1 siRNA, p-MSCV-Bmi-1 plasmid was transfected into THP-1 cells to reduce or increase the expression of Bmi-1. The expression of Bmi-1 mRNA and protein was verified by PCR and Western blot. The effect of camptothecin (CPT) on the proliferation and chemosensitivity of THP-1 cells affected by Bmi-1 gene were detected by MTT assay. The expression of DNA double-strand breaks marker-γ-H2AX was detected by immunofluorescence assay. Mitochondrial membrane potential and apoptosis were observed by flow cytometry. The expression of Cytochrome C, Caspase 3, Bax and BCL-2 was detected by Western blot. RESULTS: Silencing Bmi-1 could inhibit proliferation and enhance the sensitivity of THP-1 cells to CPT, while overexpressed Bmi-1 could promote the cell proliferation and attenucate sensitivity of THP-1 cells to CPT. Silencing Bmi-1 could enhance CPT-induced DNA double-strand breaks, decrease mitochondrial membrane potential and promote CPT-induced apoptosis. While increasing Bmi-1 gene expression could attenuate CPT-induced DNA double-strand breaks, enhamce mitochondrial membrane potential and significantly reduce CPT-induced apoptosis of cells. CONCLUSION Bmi-1 expression could influence the sensitivity of THP-1 cells to CPT, and its relative mechanism may relate to DNA double-strand breaks and endogenous apoptotic pathways.
Apoptosis ; Camptothecin/pharmacology* ; Cell Line, Tumor ; Cell Proliferation ; THP-1 Cells