Drug Delivery Systems ; Humans ; Neoplasms ; drug therapy ; etiology ; pathology ; Neoplastic Stem Cells ; pathology ; physiology ; Neovascularization, Pathologic

Animals ; Apoptosis ; Cell Cycle ; Cell Differentiation ; Cell Proliferation ; Connexin 43 ; metabolism ; Humans ; Neoplasm Invasiveness ; pathology ; physiopathology ; Neoplasms ; metabolism ; pathology ; physiopathology ; Neoplastic Stem Cells ; metabolism ; pathology ; physiology ; Neovascularization, Pathologic ; metabolism ; pathology ; physiopathology ; Receptors, CXCR4 ; metabolism ; Receptors, Formyl Peptide ; metabolism ; Vascular Endothelial Growth Factor A ; metabolism

Adenocarcinoma, Follicular ; Adult ; Carcinoid Tumor ; metabolism ; pathology ; Carcinoma, Renal Cell ; metabolism ; pathology ; surgery ; Diagnosis, Differential ; Female ; Humans ; Keratin-7 ; metabolism ; Kidney Neoplasms ; metabolism ; pathology ; surgery ; Mucin-1 ; metabolism

Antigens, CD34 ; genetics ; metabolism ; Capillaries ; metabolism ; pathology ; Genetic Heterogeneity ; Microcirculation ; Neoplasms ; blood supply ; Neovascularization, Pathologic ; genetics ; metabolism ; pathology ; Phenotype ; Platelet Endothelial Cell Adhesion Molecule-1 ; genetics ; metabolism

Animals ; Cell Proliferation ; Disease Progression ; Endothelial Cells ; pathology ; Humans ; Indoleamine-Pyrrole 2,3,-Dioxygenase ; metabolism ; Mesenchymal Stromal Cells ; immunology ; metabolism ; pathology ; Neoplasms ; immunology ; pathology ; Neoplastic Stem Cells ; pathology ; Neovascularization, Pathologic ; pathology ; Stem Cells ; pathology ; T-Lymphocytes ; immunology ; pathology

Silybum marianum (L.) Gaertn. is recorded by the pharmacopoeias of many countries to have such bioactivities as antioxidant, anti-hepatocirrhosis, anti-inflammation, immunomodulation and liver regeneration. It has been used clinically to treat hepatitis, fatty liver, liver cirrhosis, ischemic damage, radiation injury, etc. With silybin entering the phase II clinical trial as an anti-cancer drug, its correlative investigations including derivatives preparation, structure-activity relationship (SAR) detection, and the detailed action mechanisms have been developing rapidly. Recently, the new synthetic strategy of silybin and dehydrosilybin (DHS) along with their anti-HSV (herpes simple virus) activity has also been reported. This paper reviews about one hundred preparative derivatives of silybin and DHS which appeared in recent years, with the emphasis on their pharmacological activity and the results of their SAR. The future development direction of structural modification of silybin and DHS is also discussed and predicted.

Backgroud EDTA is an important calcium chelator,commonly used as an ophthalmic additive,its role in promoting corneal penetration of drug may facilitate transepithelial corneal collagen crosslinking.However,there were few reports about the penetration of riboflavin to corneal stroma enhanced by EDTA both home and abroad.Objective To determine the concentrations of riboflavin in corneal intrastromal under different interventions using high-performance liquid chromatography (HPLC).To ensure the efficacy and safety of 0.5％ EDTA in riboflavin penetrating to corneal stroma by the transepithelial procedures.Methods Fifteen New Zealand white rabbits were randomized into three groups,corneal epithelium of 5 rabbits were debrided,corneal epithelium of another 5 rabbits were left in situ and 0.5％ EDTA-Na2 was topically applied for 1 hour,and the corneal epithelium of last 5 rabbits were just left in situ,after those procedures 0.1％ riboflavin + 20％ dextran was applied topically to the corneas for 30 minutes(1 drop every 3 minutes).Then,the epithelium in the corneas of EDTA and epi-situ group were carefully removed before all the corneas were punched with an 8.5 mm diameter blade and homogenized subsequently.Ultimately,the riboflavin concentrations were determined by HPLC.Results After treated with riboflavin for 30minutes,the mean concentration of riboflavin in the epi-removed group was (23.54± 1.61)μg/g tissue,the EDTA group was (2.04 ±0.25)μg/g tissue and the epi-situ group was (1.44 ±0.06)μg/g tissue,showing significant difference among them (F =1792.839,P =0.000).There were statistically significant differences between any two groups (t =41.780,7.484,43.408,all at P ＜ 0.05).However,the riboflavin concentration of other two groups were failed to meet the theoretical value 15 μg/g tissue,excepted for epi-removed group.Conclusions The diffusion of riboflavin to the corneal stroma can be fully enhanced by removing the epithelium preoperatively,penetration enhancer EDTA can effectively promote the penetration of riboflavin to the cornea,but it still can not replace the role of removing epithelium on the penetration of riboflavin to the cornea.

Animals ; Disease Models, Animal ; Ischemic Preconditioning, Myocardial ; methods ; Myocardial Reperfusion Injury ; prevention & control ; Rats ; Rats, Wistar

Purpose:To study the expression of uPA in gestational trophoblastic cells and the relationship between uPA and the invasiveness of malignant trophoblastic tumor. Methods:73 samples from patients with gestational trophoblastic disease were harvested.In situ hybridization was employed to detect uPA expression in all of these samples. Results:① There was significant difference in the expression of uPA in cytotrophblasts among hydatidiform mole,invasive mole and choriocarcinoma(P

Objective To study the biological effect of ?3 adrenoceptor-activated electrophysiological activities in cardiac myocytes. Methods Changes of transient outward potassium ( Ito) and L-type calcium current ( ICa-L) in primary cultured guinea pig cardiacmyocytes were detected with the standard whole-cell patch clamp technique about 5 -10 min before and after administration of ?3 adrenoceptor agonist,4--2-hydroxy-( 3-chlorophenyl) ethyl-aminopropylphenoxyacetate ( BRL-37344; BRL). Results BRL could significantly increased the Ito in guinea pig cardiac myocytes with the I-V curve up-moved. When the membrane potential reached to + 80 mV,the density current increased from 6. 11 ? 1. 03 pA/pF to 8. 46 ? 2. 07 pA/pF ( n = 6,P =0. 013 064). BRL ( 10 -6mol/L) could significantly increase the ICa-Lin guinea pig cardiac myocytes,which was ( 2. 30 ?0. 75) -fold higher than in control group( n =5,P =0. 0063). When the membrane potential increased to + 10 mV ,the current,increased from 89. 25 ? 17. 83 pA to 205. 00 ? 72. 24 pA ( n = 5,P = 0. 006 3). Conclusion BRL-37344 can increase the transient Itoand ICa-L,thus further regulating the cardiac activities.