1.The study on change of serum cytokines levels and its correlation with function rehabilitation in patients with cerebral infarction
Zuoxiao LI ; Xiu CHEN ; Hua TAN ; Xianji XIONG ; Jianyi PENG
Chinese Journal of Tissue Engineering Research 2001;5(21):150-151
Objective To understand the pathogenetic role and clinical significance of interleukin 6(IL- 6 ),tumor necrosis factor(TNF) and soluble interleukin- 2 receptor(sIL- 2R) in patients with cerebral infarction(CI) .Methods The IL- 6,TNF and sIL- 2R levels were measured in 46 CI patients with ELISA. Results The serum IL- 6,TNF and sIL- 2R levels of CI group were much higher than those of controls group .The variation of IL- 6,TNF and sIL- 2R levels was closely related to the size of cerebral infarction. The serum IL- 6,TNF and sIL- 2R levels of recovery period were much lower than those of acute period in patients with CI .Conclusions The IL- 6 ,TNF and sIL- 2R take part in the pathologic process after CI .The measurement of IL- 6, TNF and sIL- 2R is useful in determining the size of cerebral infarction.
2.Recent advances on study of human breast stem cells.
Ding-bao CHEN ; Dan-hua SHEN ; Xiu KAN
Chinese Journal of Pathology 2007;36(6):423-425
Actins
;
metabolism
;
Breast
;
cytology
;
metabolism
;
Breast Neoplasms
;
metabolism
;
pathology
;
Cell Differentiation
;
Female
;
Humans
;
Keratins
;
metabolism
;
Neoplastic Stem Cells
;
metabolism
;
pathology
;
Signal Transduction
;
Stem Cells
;
cytology
;
metabolism
3.Psychosocial Factor and Immune Function of Patients with Breast Cancer before Operation
Hong CHEN ; Xiu-Ying LI ; Zhen-Hua LI ; Al ET ;
Chinese Mental Health Journal 2002;0(08):-
Objective:To compare psychosocial factors and immune function between patients with breast cancer and benign tumor before operation.Methods:The patients of breast tumor(56 case)were measured by clinical psychological rating scale(EPQ、HAMD、HAMA、LES、SSRS、DSQ)before operation,and their serum levels of IgA, CD_4~+,CD_(19)~+ and the activity of NK cell were measured.The patients of breast tumor were divided into breast cancer group(36 case)and benign tumor group(22 case)bases on results of pathology.Results:In breast cancer group, there were significantly higher scores of neurotic,total and negative life events,immature defense styles,HAMA and HAMD than those in benign tumor group;while NK cell activity,levels of CD_4~+ and CD_(19)~+ and the score of mature defense,social supports,subjective supports and utility degree of supports were marked lower.There were significantly correlation between immune function(NK cell activity,content of CD_4~+,CD_(19)~+,IgA)and psychosocial factors(social support,P score of EPQ,HAMD and HAMA total score,quantity of negative life events etc). Conclusion:Cancer patients had more server psychosocial problems and lower immune function.The immune function of patient with breast cancer before operation is associated with the various psychosocial factors.
4.Monitoring of ultrasonography in artificial vascular graft for arteriovenous fistula.
Xiu-Jie HAN ; Xiu-Hua CHEN ; Jun-Hong REN
Chinese Journal of Surgery 2008;46(9):688-690
OBJECTIVETo evaluate the monitoring of ultrasonography in artificial vascular graft for arteriovenous fistula and its complications in patients with chronic renal failure.
METHODSEighteen cases of artificial vascular graft arteriovenous fistula after four to six weeks were enrolled. The diameter, the peak velocity and blood flow were examined in arterial fistula. And the artificial vascular diameter, the peak velocity and blood flow of artificial vessels next to artery were all examined and analyzed. And the patients with symptoms in the upper extremity were tested by ultrasound.
RESULTSIntravascular blood showed good filling in fistula and artificial blood vessels in four to six weeks after artificial vascular graft for arteriovenous fistula by color doppler ultrasonography. The arterial fistula diameter, the peak velocity and blood flow were (3.61 +/- 0.68) mm and (298.56 +/- 93.42) cm/s and (583.62 +/- 216.77) ml/min. Artificial vascular diameter in (4.47 +/- 0.61) mm, the peak velocity and blood flow were (219.37 +/- 68.42) cm/s and (325.23 +/- 117.12) ml/min in the artificial blood vessels next to artery. Seven patients with upper extremity discomfort were examined by ultrasonography. One case was serum swollen. Three cases were thrombosis in artificial vessels. One case was pseudoaneurysm. Two cases were edema.
CONCLUSIONSUltrasonography plays a significant clinic role in testing artificial vascular graft for arteriovenous fistula and its complications.
Aged ; Arteriovenous Shunt, Surgical ; Blood Vessel Prosthesis Implantation ; Female ; Follow-Up Studies ; Hemodynamics ; Humans ; Male ; Middle Aged ; Postoperative Complications ; diagnostic imaging ; Postoperative Period ; Ultrasonography, Doppler, Color
5.Primary clear cell carcinoma of nasal cavity: report of a case.
Peng LI ; Wei-hua YIN ; Xiu-juan YAO ; Li WAN ; Guo-rong CHEN
Chinese Journal of Pathology 2011;40(1):52-53
Adenocarcinoma, Clear Cell
;
metabolism
;
pathology
;
surgery
;
Adult
;
Carcinoma
;
metabolism
;
pathology
;
Carcinoma, Mucoepidermoid
;
metabolism
;
pathology
;
Carcinoma, Renal Cell
;
metabolism
;
pathology
;
secondary
;
Diagnosis, Differential
;
Humans
;
Keratins
;
metabolism
;
Male
;
Nasal Cavity
;
Nose Neoplasms
;
metabolism
;
pathology
;
surgery
;
S100 Proteins
;
metabolism
7.Observation on ocular damage in trichloroethylene-induced medicamentosa like dermatitis.
Xiu-qin WANG ; Wei-xin HUANG ; Li-hua XIA ; Jia-bin CHEN ; Ying ZHANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2007;25(2):116-117
Adolescent
;
Adult
;
Dermatitis, Occupational
;
diagnosis
;
Eye Diseases
;
diagnosis
;
etiology
;
Female
;
Humans
;
Male
;
Trichloroethylene
;
poisoning
;
Young Adult
8.Antitussive constituents of Disporum cantoniense.
Xiu-Hai GAN ; Chao ZHAO ; Zhi-Yuan LIANG ; Xiao-Jian GONG ; Hua-Guo CHEN ; Xin ZHOU
China Journal of Chinese Materia Medica 2013;38(23):4099-4103
The antitussive activity assay for the root extraction of Disporum cantoniense was carried out with coughing mice induced by ammonia liquor. The results showed that the ethanol and water extractions of D. cantoniense possess strong antitussive activity, and the high dose of the former was better than positive control, and then the constituents of the ethanol extraction were separated and purified by various modern chromatographic techniques. Their structures were identified by physico-chemical properties and spectroscopic data. As a result, eight compounds were isolated and identified as stigmast-4-en-3-one(1), (22E, 24R)-ergosta-5, 7, 22-trien-3beta-ol(2), obtucarbamate A(3), obtucarbamate B(4), neotigogenin(5), azo-2, 2'-bis[Z-(2,3-dihydroxy-4-methyl-5-methoxy) phenyl ethylene] (6),dimethyl {[carbonylbis (azanediyl)] bis( 2-methyl-5, 1-phenylene) j dicarbamate (7) , and quercetin-3-O-pB-D-glucopyranoside(8). All compounds were isolated from this plant for the first time, and the result of bioactivity-directed isolation showed that compounds 3, 4, and 6 had obvious effect on antitussive activity, and compound 6 had the same level as positive control.
Animals
;
Antitussive Agents
;
chemistry
;
isolation & purification
;
pharmacology
;
Drugs, Chinese Herbal
;
chemistry
;
isolation & purification
;
pharmacology
;
Ethanol
;
chemistry
;
Female
;
Liliaceae
;
chemistry
;
Male
;
Mice
9.Study on Inducing of Cord Blood Derived Endothelial Progenitor Cells into Endothelial Cells
xiu-hua, WANG ; bing, JIA ; zhang-gen, CHEN ; ke-qiang, WANG
Journal of Applied Clinical Pediatrics 2006;0(20):-
Objective To induce cord blood derived endothelial progenitor cells(EPCs)into endothelial cells,and investigate the feasibility of these cells as the seed cells of tissue engineering cardiovascular replacement.Methods Mononuclear cells were isolated from fresh cord blood by 6%HES and density gradient centrifugation.Isolated cells were cultured in medium supplemented with vascular endothelial growth factor(VEGF).Attached cells were identied by morphology,immunofluorescence staining and flow cytometry.Results The percentage of mononuclear cells isolated from fresh cord blood was(3.4?2.1)?10~7/mL.The morphology of attached cells changed while being cultured and inducted,from small-sized round cells to spindle-like cells,to a typical cobblestone morphology,and the total number of cells was 10~7.After 7 days of culture,immunofluorescence staining showed that the vWF and VEGFR-2 were expressed.Compared with the original,cell markers CD_133 decreased(3.11%?1.05%) to(0.09%?0.02%),P
10.PRELIMINARY STUDY ON AN ANTIBIOTIC-PRODUCING BACTERIUM
Xi-Qian LAN ; Jun-Hua HU ; Hong-Xiu WEN ; Jia-Lian CHEN ; Ze-Yang ZHOU ;
Microbiology 1992;0(05):-
An antibiotic-producing bacterium, which was numbered as 20 #-5, was separated from the soil in Chongqing. It was identified as the member of pseudomonas. Gram positive bacteria are badly suppressed by it. The antibiotic secreted by 20 #-5 can endure 100℃ for half an hour, and it can also go through the ultrafiltration membrane with pores of 0.22?m.