Objective To evaluate the short-term effect of leuprorelin acetate microspheres in preventing recurrence of ovarian endometrioma after conservative surgery.Methods From January 2011 to September 2011,190 ovarian endometrioma patients undergoing conservative laparoscopic surgery at Affiliated Obstetrics and Gynecology Hospital Affiliated to Fudan University were enrolled in this retrospective study.Among 184 patients were followed up,the range of following up were 12 to 21 months.116 cases presented dysmenorrheal.Based on postoperative treatment,they were classified into 124 cases treated by domestic gonadotropin releasing hormone agonist (GnRH-a) post-operatively for 3-6 months and 60 cases without postoperative treatment.Among all,63 patients were treated with,that was leuprorelin acetate microspheres for injection (Beiyi,3.75 mg,q28 d),61 patients were treated with imported GnRH-a post-operatively for 3-6 months,that were either Zoladex (3.6 mg,q28 d),Dophereline (3.75 mg,q28 d) or Enatone (3.75 mg,q28 d).The recurrence and pain improvement were compared among those groups.Results (1) The total rate of cyst recurrence was 12.5％ (23/184) while the average recurrent time was (13.7 ±2.6) months (2-21 months).The cyst recurrence rate was significantly lower in patients treated with GnRH-a post-operatively than those who didn't take medications [21.7％ (13/60) versus 8.1％ (10/24),P ＜ 0.05].However,there was no significant difference between domestic GnRH-a group and the imported one [7.9％ (5/63) versus 8.2％ (5/61),P ＞ 0.05].(2) After conservative surgery,symptoms were found to be relieved in 87.1％ (101/116) patients among 116 patients complaining of dysmenorrheal preoperatively and the pain recurrence rate was 12.9％ (13/101).However,there was no significant difference in either symptom relief rate or pain recurrence rate among different groups.The symptom relief rate were 87％ (33/38),86％ (37/43) and 89％ (31/35) while the pain recurrence rate were 12％ (4/33),14％ (5/37) and 13％ (4/31) respectively in none,imported GnRH-a group and domestic GnRH-a group.Conclusions Leuprorelin acetate microspheres could be effective in preventing recurrence of ovarian endometrioma,but not in symptom relieving after conservative surgery in short term.The effect of domestic and imported GnRH-a was similar.

Objective To investigate the efficiency of antitumor immune responses induced by a controlled live dendritic cell(DC)vaccine Methods DC precursors were isolated from Fischer 344 rat bone marrow and cultured with granulocyte macrophage colony stimulating factor and interleukin 4 The rat ovarian tumor cell line NuTu 19 was genetically modified by retroviral mediated suicide gene(HSV 1 TK), and the positive clones were selected using G418 Live DC vaccine was then fused with DC and NuTu 19/TK cell by polyethylene glycol The characteristics of live DC vaccine were assayed with flow cytometry and confocal laser scanning microscopy The specific expression of HSV 1 TK gene in live DC vaccine was evaluated by RT PCR and western blot The sensitivity of live DC vaccine to ganciclovir (GCV) was evaluated by methylthiazoletetrazolium assay In vivo, rats vaccinated twice with live DC vaccine were compared to those vaccinated with killed DC vaccine, unfused DC and NuTu 19/TK cell or phosphate buffered saline Seven days following the last immunization, the rats were sacrificed to test the specific cytotoxic T lymphocyte (CTL) activity by lactate dehydrogenase release assay, or challenged with NuTu 19 and tumor incidence was observed Results The fusion efficiency was approximately (23?14) Live DC vaccine displayed an up regulated expression of major histocompatibility complex (MHC) IIOX6 (87 6?3 4)%, costimulatory molecule B 1 2 (71 1?9 3)%, integrin OX 62 (68 0?7 4)%, and adhesion ICAM 1 (77 1?2 0)%, and specifically expressed HSV 1 TK gene. Our data showed that spleen T lymphocytes from rats vaccinated with live vaccine displayed enhanced CTL aetivity (61 8?8 3)% contrast to that of rats vaccinated with killed vaccines (26 0?3 8)% ( P

Background Current researches of choroidal structure changes primarily focus on macular choroidal thickness in adolescents and children,but there are few studies on peripapillary choroidal thickness in children with enhanced depth imaging (EDI)-OCT.In addition,the reliability of manual measurements to peripapillary choroidal thickness is an important factor for the estimation of EDI-OCT.Objective This study was to determine the repeatability and reproducibility of manual measurements of peripapillary choroidal thickness in children by EDI-OCT.Methods A reliability evaluation of diagnosis test was performed.EDI-OCT technology was used to image the peripapillary choroidal thickness in 49 children aged 7-14 years in Eye Hospital of Wenzhou Medical University from February to March 2015 under the informed consent of their custodians.An annular scanning was carried out surrounding optical disc by the same ophthalmologist with Spectralis OCT,and the peripapillary choroidal thickness was manually measured at global,temporal,superotemporal,superonasal,nasal,inferonasal and inferotemporal zones.The intraclass and interclass repeatability and reducibility of measuring values from intraobserver,inter-observer and intra-session were evaluated with intraclass correlation coefficients (ICC),and BlandAltman agreement analysis was used to assess the consistency of repeated measurements.Results The average age in the subjects was (9.9 ± 1.3) years.No significant difference was found in the mean difference of reduplicative measured peripapillary choroidal thickness at each zone (all at P＞0.05).The ICCs of intra-observer,inter-observer and intra-session were 0.971-0.993,0.827-0.952 and 0.974-0.991,respectively,and the 95％ limit of agreement of global peripapillary choroidal thickness were-12.4-9.7 μm,-15.2-11.6 μm and-16.3-19.1 μm,respectively.Conclusions The repeatability,reproducibility and consistency are favorable in manually measured values of peripapillary choroidal thickness from EDI-OCT image.EDI-OCT appears a good application in the assessment of peripapillary choroidal thickness in children.

Humans ; Hypertension, Pulmonary/surgery* ; Learning Curve ; Angioplasty, Balloon ; Pulmonary Embolism/therapy* ; Chronic Disease ; Pulmonary Artery/surgery*