[Objective] To assess the safety of patients with pheochromocytoma receiving iodinated contrast media injection during enhanced CT examination.[Methods] The systolic pressure,diastolic pressure,heart rate of 205 patients with suspected diagnosis of adrenal pheochromocytoma were continuously recorded 10 minutes before the examination,and 5 minutes,10 minutes,20 minutes,30 minutes,and 24 hours after the examination.Among them,50 patients whose diagnosis were confirmed by pathology as pheochromocytoma were set as group A.Others were excluded pheochromocytoma and 50 were extracted from them and named as the control group(group B).The changes of blood pressure and heart rate were analyzed at different time points.[Results] The changes of blood pressure and heart rate before and after the examination in two groups showed no significant difference,as well as those at different time points between two groups.[Conclusions] Intravenous injection of low-osmolar iodinated contrast media does not induce changes of blood pressure and heart rate in pheochromocytoma patients.Pheochromocytoma patients have the same safety as patients with other adrenal carcinoma during enhanced CT examination.

Objective:To approach the significance of serum C-Reactive protein(CRP) and matrix metalloproteinases-9(MMP-9) levels in the diagnosis unstable angina(UA) and its forecasting of prognosis .Methods:The serum CRP levels of 38 cases of UA,36 cases of stable angina(SA) and 35 cases of normal controllers was measured by immunoturbidimetric method.And meanwhile,the serum MMP-9 levels of the above-mentioned three groups was measured by enzyme linked immunosorbent assay(ELISA).Results:When the groups were hospitalized,the CRP levels of the UA group(5.85?2.23 mg/L)was evidently higher than that of the SA group(2.54?1.07 mg/L)and the normal control group(1.21?1.02 mg/L)( P

Objective To explore the level of soluble intercellular adhension molecule-1 (sICAM-1) in the patients with acute ischemic cerebrovascular disease (AICVD) and its clinical significance. Methods The level of sICAM-1 was determined by double-antibody enzyme linked immunosorbent in 67 patients with AICVD and 45 patients being susceptible to AICVD. 40 healthy subjects served as normal control group. Results The level of sICAM-1 in the patients with AICVD was significantly higher than that in the patients being susceptible to AICVD (P

Objective To explore the effect and mechanism of fluvastatin preventing and curing restenosis. Methods 48 rabbits were randomly divided into 3 groups. In control group, rabbits were given basic food. In balloon injury group, rabbits were given basic food and balloon injury of general artery on right neck. In fluvastatin balloon group, rabbits were given basic food,ballon injury of general artery and 10mg?kg -1 ?d -1 fluvastatin. The expression of MMP-9 and TIMP-2 of mRNA was detected at 3, 7, 14 and 30 days after injury respectively by method of in site hybridization. Results There was little expression of MMP-9 and TIMP-2 mRNA in control group, and the expression of MMP-9 and TIMP-2 mRNA in middle membrane of blood vessels began at 3 days after blood injury, and reached maximum at 7 days after injury. There was a little expression of MMP-9 and TIMP mRNA in inner membrane of blood vessels at 14 and 30 days after injury, and MMP-9 expression significantly decreased after the fluvastatin interference(P

Inquisition language,as the information carrier on observing and describing disease status,makes it possible for patients to communicate well with doctors to acknowledge disease information.In order to make inquisition language possess specific properties of medical conversation,doctors need to master language skills in procedural inquisition,proper listening and responding to inquisition,distinguishing inquisition,transitional inquisition and secret-involved inquisition.Upholding inquisition language with courtesy,comfort,being explanatory,encouraging,and protecting is the basic requirement of clinical ethics.

ObjectiveTo study the modes of case finding and diagnosis for inpatients of active pulmonary tuberculosis with primary treatment.MethodsData of 1000 inpatients with active pulmonary tuberculosis input into a computer were analyzed retrospectively.including clinical symptoms,signs and relevant laboratory examinations.to evaluate their diagnostic value.ResultsAmong 1000 active tuberculosis case8 hospitalized with symptoms and signs,95.9 percent suffered by cough,77.7 percent by expectoration and 50.8 percent (n=508) by fever,and 51.5 percent (n=777) with strong positive purified protein defivative (PPD) skin test,61.5 percent with positive serum anti-tuberculosis antibody,48.8 percent with positive acid-fast staining on sputum smear and 57.9 percent with positive sputum bacteriologle culture.And,49.4 percent of the patients were diagnosed by laboratory positive sputum,and 50.6 percent of those with negative sputum were diagnosed by comprehensively clinical considerations,ineluding 51.6 percent positive PPD skin teat, or positive serum anti-tubereulosis antibody,but 48.4 percent of them were all negative in varied laboratory examinations.ConclusionsHospital visit due to symptom is the main method for tuberculosis finding in our country.All those with Cough for two or more weeks should be screened by routine examinations for excluding tuberculosis.Case finding rate Was low by sputum examinations.so comprehensive diagnosis is still important for those tuberculosis patients with negative sputum.

OBJECTIVE To understand the present situation of hand hygiene execution rate among clinical medical personnel,and discuss a series of scientific,reasonable and practical hand hygiene intervention methods,in order to elevate the hand hygiene execution rate among medical personnel.METHODS Using the self-designed questionoaires in combination with observation on hand hygiene process and investigation of hand hygiene knowledge level among medical personnel in various departments of different levels of hospitals to assess the hand hygiene execution rate.RESULTS The investigation indicated 76.00% medical personnel have had hand hygienic knowledge training,and referred in the stipulation health drafts,the hand hygiene execution rate was 50.00-70.00%.The hand health execution rate before contact with patients was 59.82%,and after contacts with patients was 77.97%;among department the hand hygiene execution rate before contact with patients was highest in the department of pediatrics(82.78%),the lowest was in emergency department(11.11%),after contact with patients the highest was department of infectiong(100.00%),and the larest was in the internal medicine department(39.93%).CONCLUSIONS Medical personnel's hand hygiene consciousness is still weak,with lacked hand hygiene related knowledge,it is necessary to summarize a set of reasonable effective intervention plans to elevate the hand hygiene execution rate,reduce the hospital infection percentage,and lower the patient pain and the economic loss.

Purpose To investigate the expression of tumor buds and its relationship with clinicopathological characteristics in endome-trial carcinoma. Methods 47 cases of endometrial carcinoma were observed and analyzed by means of clinicopathologic data and im-munohistochemical staining. The connections between tumor buds and clinicopathologic parameters were studied by statistics. Results Of the 47 endometrial carcinoma cases, tumor buds were seen in 18 cases. Tumor buds were correlated with histological grade, lymph node metastases, vascular invasion, Ki-67 index and survival. There were no associations between tumor buds with age, tumor size, pTNM stage or myometrial invasion depth. Immunohistochemistry demonstrated that the level of N-cadherin, vimentin in bud cells was higher than normal carcinoma cells while the level of CK(AE1/AE3),β-catenin and E-cadherin got the opposite results. Conclu-sion Tumor buds may play an important role in the progression of endometrial carcinoma. The immunohistochemical features of bud cells indicated that tumor buds may be a key step in epithelial-mesenchymal transition.

Objective To develop the technique to detect total core antigen of HCV(Total HCV-cAg) by Enzyme-Linked Immu-nosorbent Assay (ELISA) and apply it for clinical diagnosis. Methods 201 serum samples with anti-HCV antibody were detected total HCV-cAg after pre - treating the samples, then the sensitivity of results were compared with HCV RNA tests. Among them, 176 cases was determined by FQ-PCR, and 25 cases by RT-PCR for HCV-RNA. Results HCV RNA was found in sera from 88 of 201 samples (43.8%). Total HCV-cAg was positive in 71 (35.3%) of 201 samples . There was no significant difference between the detection rate of HCV RNA by PCR and total HCV-cAg by ELISA. Conclusion Detection of total core antigen of HCV is suitable to be used as to diagnose HCV in clinic.

OBJECTIVETo observe the effect of combined use of jinnaduo (an injection made by extract of Ginkgo leaf, EGb) and Deferoxamine (DFO, a chelating agent) in antagonizing the ototoxicity of cisplatin (CDDP).

METHODSGuinea pigs were randomly divided into the CDDP group, the EGb group, the DFO group, the combined treated group (EGb + DFO) and the control group. Changes of auditory brain-stem response (ABR), serum superoxide dismutase (SOD) activity and malondialdehyde (MDA) content, as well as light and scanning electronic microscopic (SEM) figures were observed before and after treatment.

RESULTSThe threshold of ABR was significantly higher in the CDDP group than that in the other groups (P<0.01), but was insignificantly different among the latter groups (P>0.05). Serum SOD activity was lower and MDA content was higher in the CDDP group than those in the control group (P<0.01), but in comparison of the two parameters between control and other groups, the difference was insignificant (P>0.05). SEM examination on cochlea showed that the damage of hair cells was milder in the DFO group and the combined treated group than that in the CDDP group, which was slightly milder in the EGb group than that in the CDDP group.

CONCLUSIONCombined use of EGb and DFO could effectively reduce the ototoxicity of CDDP, its effect is better than using EGb singly, and similar to that of using DFO alone. The combination could also prevent the side-effect of CDDP in bone marrow inhibition. The Fe ion participated free radical response could be one of the mechanisms of CDDP in damaging hearing.

Animals ; Cisplatin ; adverse effects ; Cochlea ; drug effects ; Deferoxamine ; pharmacology ; Drug Synergism ; Drugs, Chinese Herbal ; pharmacology ; Evoked Potentials, Auditory, Brain Stem ; Female ; Ginkgo biloba ; chemistry ; Guinea Pigs ; Male ; Malondialdehyde ; blood ; Random Allocation ; Superoxide Dismutase ; blood