Three active components were isolated from the metabolite of Pseudomonas sp.20~(#)-5 by using organic solvent extraction,silica gel column chromatogram and C-18 reverse phase chromatogram in HPLC.Two of them were identified as phenazine-1-carboxylic acid and 3-n-heptyl-3-hydroxy-1,2,3,4-tetrahydroquinoline-2,4-dione respectively.