1.Separation of total coumarin in Artemisia capillaris by solvent sublation
Chinese Traditional and Herbal Drugs 1994;0(05):-
Objective An effective method for separating coumarin from Artemisia capillaris by solvent sublation was established.Methods The effects of sublation solvent,the concentration of sample solution,N2 gas flow rate,pH value of solution,sublation time,and electrolyte NaCl etc.on the sublation efficiency were investigated and the optimal conditions of the solvent sublation were obtained.Results In the optimal conditions,the results of the solvent sublation were evaluated and compared with the solvent extraction.Conclusion The experimental results show that this method is simple and rapid,and the efficiency of coumarin by solvent sublation is far better than that by the solvent extraction.
2.Inhibitory effects of high mobility group chromosomal protein N2 on human tongue carcinoma transplanted in nude mice.
Xiqian LIU ; Xiaoqian DONG ; Yonghong ZHANG ; Ping ZHANG ; Xiaoyu LI ; Sixiu CHEN ; Yun FENG
West China Journal of Stomatology 2014;32(1):5-8
OBJECTIVETo evaluate the inhibitory effect of high mobility group chromosomal protein N2 (HMGN2) on human tongue carcinoma tumor in nude mice.
METHODSA transplantation tumor model in nude mice was constructed by injecting Tca8113 cells. After a week, negative control groups, masculine control groups, and HMGN2 groups were established. Cell culture of the three groups were separately injected with washing buffer II, cis-dichlorodiamineplatinum (DDP), and HMGN2 protein. The tumors were moved after four treatments, and then analyzed by hematoxylin-eosin (HE) staining.
RESULTSA transplanted tumor model was established successfully. The volumes of HMGN2 groups and masculine control groups were smaller than those of the negative groups. Mouse weight did not differ among the three groups. Average tumor weight of the negative group was (0.38 +/- 0.19)g, that of the HMGN2 group was (0.21 +/- 0.15)g, and that of the DDP group was (0.23 +/- 0.16)g. These factors indicated no statistically significant difference among the three groups. The tumor inhibitory rate of HMGN2 group was 45.71%, and that of the positive group was 39.44%. Based on evaluation by naked eye, the tumor in the negative group was larger than that in other groups. In addition, cell necrosis was observed during HE staining.
CONCLUSIONHMGN2 could significantly inhibit growth of the transplanted tumor in nude mice.
Animals ; Carcinoma ; Cell Line, Tumor ; High Mobility Group Proteins ; Humans ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Neoplasm Transplantation ; Tongue Neoplasms
3.Study on prohibition of high mobility group chromosomal protein N2 against human oral squamous cell carcinoma in vitro.
Xiaoqian DONG ; Xiqian LIU ; Yonghong ZHANG ; Ping ZHANG ; Libing LU ; Xiaoyu LI ; Ping HUANG ; Yun FENG
West China Journal of Stomatology 2013;31(1):91-95
OBJECTIVETake human oral squamous cell carcinoma Tca8113 as experimental model, and study the anti oral squamous cell carcinoma activity of high mobility group chromosomal protein N2 (HMGN2) molecule.
METHODSTrain a large number of recombinant human HMGN2 expression vector Escherichia coli BL21. HMGN2 was expressed under isopropyl-1-thio-beta-galactopyranoside (IPTG) induction and purified by B-PER GST Fusion Protein Purification Kit. A variety of concentrations HMGN2 were added to cell culture medium, cells were tested by MTT, Hoechst 33342 fluorescence staining, flow cytometry assay and Western-blot.
RESULTSMTT results proved that HMGN2 could significantly inhibit human oral squamous cell carcinoma Tca8113 growth. Hoechst 33342 fluorescence staining, flow cytometry assay test and Western-blot proved HMGN2 could make Tca8113 cells morphological change, make Tca8113 cells block in S period of cell cycle and strongly promote Tca8113 cells to apoptosis.
CONCLUSIONHMGN2 can promote apoptosis of oral squamous cell carcinoma cells.
Apoptosis ; Carcinoma, Squamous Cell ; Cell Proliferation ; High Mobility Group Proteins ; Humans ; In Vitro Techniques ; Mouth Neoplasms ; Recombinant Proteins
4.Influence of different surgeries on growth and development of alar cartilage in young-rabbit.
Lian JIANG ; Xiqian DONG ; Qinggao SONG ; Shang CHEN ; Sihai ZOU
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2011;25(2):74-77
OBJECTIVE:
The purpose of this study is to observe the affection of different clinical surgeries on alar nasal cartilages' growth and development. The experimental results can provide some theory basis for clinical surgeries.
METHOD:
Twenty-eight New Zealand immature rabbits were used in this study, and divided into normal control group, hidden dissection group and cutting off alar nasal cartilages group randomly, which included 4,12 and 12 rabbits, separately. Arc incision were made on the mucous membrane of nasal cavity,and then dissect the alar nasal cartilages hidden or cut off the alar nasal cartilages, separately. The growth and development of the alar cartilage were observed at different stages after the surgery using histological and immuno-histochemical methods.
RESULT:
Four weeks, eight weeks, twelve weeks and sixteen weeks after surgery, there were no significant differences in the indexes of chondrocytes between hidden dissection group and control group. In cutting off alar nasal cartilages group, fiber tissue were observed in the vacancy left after being cut off cartilages, and even mucous membrane tissue could be seen in some slices.
CONCLUSION
There is no adverse influence on the growth and development of the alar cartilage after being hidden dissected. Contrarily, the restoring capability of transparent cartilage cannot counteract the injury resulted form the surgery after the alar nasal cartilages being cut off.
Animals
;
Nasal Cartilages
;
growth & development
;
surgery
;
Nose
;
surgery
;
Rabbits
;
Rhinoplasty
;
methods