Objective To investigate the inhibitory effect of rosuvastatin on the migration and invasion of U87 glioma cells and its related mechanisms. Methods Cultured U87 cells were treated with 0, 5, 10 and 20 μmol/L rosuvastatin for 24,48 and 72 h. Cell viability was measured by CCK-8 assay. Migration ability of the cells was detected by scratch assay,and invasion ability of the cells was detected by Transwell assay. The expression of matrix metalloproteinase 2(MMP2),MMP9 and the Wnt/β-catenin signaling pathway-related proteins was detected by Western blot assay. Results Compared with the control group,the viability of U87 cells in the 5,10 and 20 μmol/L rosuvastatin groups was decreased (P < 0.01)in a time- and dose-dependent manner. Migration and invasion abilities of the cells were decreased(P <0.01). In addition,the expression levels of MMP2,MMP9,Wnt1,Wnt3a,Wnt7a,and β-catenin were decreased as well (P < 0.01). Conclusions Rosuvastatin can significantly inhibit the migration and invasion abilities of U87 glioma cells, probably related to the blocking of Wnt/β-catenin signaling pathway.

Objective: To investigate the current status of prevention and control of occupational hazards in a city in 2017, to understand the capability to prevent and control occupational hazards and the level of occupational health supervision and management, and to propose measures to urge employers to assume the main responsibility for the prevention and control of occupational diseases. Methods: An analysis of the main factors influencing the prevention and control of occupational hazards in the city was performed to screen out six semi-quantitative assessment indicators (including the training of the main responsible persons and occupational health management personnel in companies) and four qualitative assessment indicators (including the coverage of supervision and inspection of occupational hazards performed by the district safety supervision department) , which could be used to measure the prevention and control effects of regional occupational hazards. Each indicator was scored. The typical investigation method was used to do data review and on-site inspection of 170 companies, 17 district-level occupational health supervision departments, and 16 sub-district (township) occupational health supervision departments in the city from October to December, 2017. The prevention and control of occupational diseases in each district was scored, and the completion rate and completion situation of each indicator in the city were analyzed. Results: The mean score of prevention and control of occupational hazards in the city was 84.9. The scores of two districts were relatively high, being 88.9 and 88.7, respectively; the scores of 9 (52.9%) districts were higher than 85. The pass rate of training for the main responsible persons and occupational health management personnel in companies in the city was 95.9%, the pass rate of occupational health training for workers was 84.7%, the pass rate of occupational health examination for workers was 96.5%, the pass rate for the inspection of occupational hazardous factors in workplaces was 95.3%, the pass rate for notifying occupational hazards in workplaces is 95.9%, and the pass rate for applying the warning signs of occupational hazards in workplaces was 76.5%. Conclusion The awareness of the importance of prevention and control of occupational hazards in all districts of the city has been increased, but the effectiveness of occupational health training for workers needs to be strengthened. The supply and demand of occupational health technical services are still not matched, and the ways and methods of occupational health supervision and inspection need to be improved.

OBJECTIVE:To investigate clinical efficacy of Sofren injection combined with Vinpocetine injection in the treatment of acute massive cerebral infarction,and its effects on hemorheological indexes and serum NOS.METHODS:A total of 60 patients with acute massive cerebral infarction in our hospital during Jan.2014-Jun.2016 were selected as research objects and divided into trial group and control group according to random number table,with 30 cases in each group.Control group was given Citicoline injection 0.5 g,ivgtt,qd.Trial group was additionally given Vinpocetine injection 20 mg added into 0.9％ Sodium chloride injection 250 mL,ivgtt,qd;1 h later washing tube,they were given Sofren injection 10 mL added into 0.9％ Sodium chloride injection 250 mL,ivgtt,for consecutive 14 d.Clinical efficacies and safety of 2 groups were observed,and hemorheological indexes and NOS levels were observed before and after treatment.RESULTS:The total response rate (83.33％)of trial group was significantly higher than that (50.00％) of control group,with statistical significance (P＜0.05).Before treatment,there was no statistical significance in hemorheological indexes or serum NOS levels between 2 groups (P＞0.05).After treatment,hemorheological indexes of 2 groups were decreased significantly,and the trial group was significantly lower than the control group.The level of serum NOS in 2 groups were increased significantly,and the trial group was significantly higher than the control group,with statistical significance (P＜0.05).No obvious ADR was found in 2 groups.CONCLUSIONS:Sofren injection combined with Vinpocetine injection show significant therapeutic efficacy for acute massive cerebral infarction,can reduce blood viscosity and increase blood perfusion with good safety.

Objective To explore expression and significance of growth differentiation factor (GDF)-15 in pituitary adenomas.Methods A total of 100 tissue samples of patients with pituitary adenomas were selected and divided into invasive pituitary adenomas group (n =63) and non-pituitary adenomas group (n =37) according to aggressive condition,and 50 healthy people were selected as control group.The expression of GDF-15 was compared among the three groups,and the correlation between GDF-15 and the invasiveness of pituitary adenomas was analyzed.Results The level of GDF-15 in patients with prolactin secreting pituitary adenomas and growth hormone adenomas was significantly higher than that of patients with non-functional pituitary adenomas (P ＜ 0.05).The level of GDF-15 in the invasive pituitary adenomas group was significantly higher than the non-invasive group and the control group (P ＜ 0.05).The correlation analysis showed that there was a positive correlation between GDF-15 and invasive pituitary adenomas (r =0.328,P =0.001).Conclusion In patients with pituitary adenomas,the expression of GDF-15 is higher,and there is a correlation between the GDF-15 level and the invasiveness of pituitary adenomas.

Objective To explore expression and significance of growth differentiation factor (GDF)-15 in pituitary adenomas.Methods A total of 100 tissue samples of patients with pituitary adenomas were selected and divided into invasive pituitary adenomas group (n =63) and non-pituitary adenomas group (n =37) according to aggressive condition,and 50 healthy people were selected as control group.The expression of GDF-15 was compared among the three groups,and the correlation between GDF-15 and the invasiveness of pituitary adenomas was analyzed.Results The level of GDF-15 in patients with prolactin secreting pituitary adenomas and growth hormone adenomas was significantly higher than that of patients with non-functional pituitary adenomas (P ＜ 0.05).The level of GDF-15 in the invasive pituitary adenomas group was significantly higher than the non-invasive group and the control group (P ＜ 0.05).The correlation analysis showed that there was a positive correlation between GDF-15 and invasive pituitary adenomas (r =0.328,P =0.001).Conclusion In patients with pituitary adenomas,the expression of GDF-15 is higher,and there is a correlation between the GDF-15 level and the invasiveness of pituitary adenomas.

Objective:To explore the effect and mechanism of diosmetin (Dio) on neuronal ferroptosis in rats with bacterial meningitis (BM).Methods:Male SD rats aged 6-7 weeks of SPF grade were selected for the experiment. The BM model was established by injecting group B hemolytic streptococcus into the cisterna magna of cerebellum. Sixty BM model rats were successfully modeled and divided into model group, low-dose Dio group, medium-dose Dio group, high-dose Dio group and inhibitor group according to the random number table method, with 12 rats in each group. Another 12 weight-matched rats were taken as the control group.The rats in the low-dose Dio group, medium-dose Dio group, high-dose Dio group and the inhibitor group were intragastrically administered with Dio at 50 mg/kg, 100 mg/kg, 200 mg/kg and 200 mg/kg, respectively. The rats in the control group were intragastrically administered with an equal volume of 0.9 % sodium chloride solution. On the day of intragastric administration, the rats in the inhibitor group were intraperitoneally injected with SIRT1 pathway inhibitor EX527 (10 mg/kg), and the rats in the other groups were injected with an equal volume of 0.9% sodium chloride solution. The above interventions were performed once a day for 28 consecutive days. Loeffler neurological score was used to evaluate the neurological impairment in rats. Interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in cerebrospinal fluid of rats were detected by ELISA. The number of white blood cells in cerebrospinal fluid was detected by a blood cell analyzer. Glutathione (GSH) was detected by micro-enzyme labeling method, malondialdehyde (MDA) was detected by thiobarbituric acid colorimetric method, reactive oxygen species(ROS) was detected by colorimetry, and Fe 2+ level was detected by ferrozine method. Hematoxylin-eosin staining, Prussian blue staining and TUNEL staining were used to observe the pathological damage, iron accumulation and apoptosis in the hippocampus, respectively.Western blot was applied to measure the expression of transferrin (Tf), proliferating cell nuclear antigen (PCNA), Bcl-2-associated X protein (Bax), caspase-3 and SIRT1/Nrf2/HO-1/Gpx4 signaling pathway proteins. Graphpad Prism 9.0 was used for data analysis. One-way ANOVA was used for statistical analysis, and SNK- q test was used for further pairwise comparisons. Results:(1) There was a statistically significant difference in neurological function scores among the 6 groups of rats ( F=125.451, P<0.001). The neurological function score of the model group was lower than that of control group, while the neurological function scores of the low-dose Dio group, medium-dose Dio group, and high-dose Dio group were higher than those of the model group (all P<0.05). The neurological function score of the inhibitor group ((2.57±0.26)) was lower than that of high-dose Dio group ((4.34±0.48)) ( P<0.05). (2) There were statistically significant differences in the levels of IL-6, TNF-α and the number of white blood cells in the cerebrospinal fluid of rats among the 6 groups ( F=127.817, 102.413, 180.967, all P<0.001). The levels of IL-6, TNF-α and the number of white blood cells in model group were higher than those of control group(all P<0.05). The levels of IL-6, TNF-α and the number of white blood cells in low-dose Dio group, medium-dose Dio group and high-dose Dio group were lower than those of model group (all P<0.001), and those in inhibitor group were all higher than those in high-dose Dio group(all P<0.001). (3) There were statistically significant differences in iron deposition rate and neuronal apoptosis rate among the 6 groups of rats ( F=90.857, 88.835, both P<0.001). The iron deposition rate ((18.37±3.14)%) and neuronal apoptosis rate ((27.58±2.63)%) in the inhibitor group were higher than those in the high-dose Dio group ((6.35±1.08)%, (14.02±1.87)%) (both P<0.05). (4) The levels of GSH, ROS, MDA, and Fe 2+ in the hippocampus of the 6 groups of rats showed statistically significant differences ( F=54.465, 106.453, 55.969, 105.457, all P<0.001). The GSH content in the inhibitor group ((103.48±8.76) mmol/g) was lower than that in the high-dose Dio group ((133.97±10.54) mmol/g), while the contents of ROS, MDA, Fe 2+ ((225.17±16.32) μmol/mg, (10.73±1.58) μmol/mg, (62.71±5.43) μg/g) were higher than those of the high-dose Dio group ((131.87±11.67) μmol/mg, (4.35±0.87) μmol/mg, (34.86±2.95) μg/g) (all P<0.05). (5)There were statistically significant differences in the protein levels of Tf, PCNA, Bax, caspase-3, SIRT1, Nrf2, HO-1 and Gpx4 in the hippocampus of the 6 groups of rats ( F=120.179, 107.568, 157.265, 98.031, 90.932, 52.283, 59.424, 114.539, all P<0.001). The protein levels of Tf, Bax and caspase-3 in the hippocampus of inhibitor group were higher than those of the high-dose Dio group, while the protein levels of PCNA, SIRT1, Nrf2, HO-1, Gpx4 were lower than those of the high-dose Dio group (all P<0.05). Conclusion:Diosmetin can activate SIRT1/Nrf2/HO-1/Gpx4 signaling pathway, thereby inhibiting neuronal ferroptosis in BM rats.

Objective To explore application of nursing intervention of children's fun mode m anesthesia induction of preschool children.Methods A total of 180 preschool children undergoing elective surgery in our hospital were randomly divided into control group and observation group according to the random number table method,with 90 cases in each group,the control group was given routine nursing,and the observation group with children's fun mode nursing intervention,the curative effect is compared between the two groups.Results Before operation,there was no significant difference between the two groups in the improvement of the perioperative anxiety scale (mYPAS) score in the groups (P ＞ 0.05).During induction of anesthesia,mYPAS score of the two groups before operation were significantly higher than one day before surgery,and the differences were statistically significant (P ＜ 0.05),and anxiety score in the observation group was significantly lower than the control group (P ＜ 0.05).Before one day of operation,there was no significant difference in systolic blood pressure (SBP) and diastolic blood pressure (DBP) between the two groups (P ＞0.05).During induction of anesthesia,the SBP,DBP of two groups were significantly higher than one day before operation,and the differences were statistically significant (P ＜ 0.05),and the observation group had lower SBP and DBP than the control group,the difference was statistically significant (P ＜ 0.05).Before one day of surgery,there was no significant difference in heart rate (HR) between two groups (P ＞ 0.0 5).During induction of anesthesia,the two groups had higher HR than one day before operation (P ＜ 0.05),and the observation group had lower HR than the control group (P ＜ 0.05).The observation group during anesthesia induction had lower cooperation scale (ICC) score than the control group(P ＜ 0.05).Conclusion Nursing intervention of children's fun mode for preschool children can improve children's blood pressure,and has less influence on HR so as to improve cooperation of anesthesia induction.

Objective To evaluate the application of peritoneal shunt in lumbar cistern in the treatment of communicating hydrocephalus.Methods Fifty patients with communicating hydrocephalus were randomly divided into observation group and control group.Patients in control group were treated by ventricle-peritoneal shunt (V-P),and patients in observation group were treated by lumbar cisterna-peritoneal shunt (L-P),the application effect of the two treatment methods for communicating hydrocephalus were compared.Results Compared with V-P shunt,L-P shunt has better clinical effect and lower complication rate.Conclusion The lumbar cisterna shunt has good therapeutic effect in the treatment of traffic hydrocephalus.

Objective To explore application of nursing intervention of children's fun mode m anesthesia induction of preschool children.Methods A total of 180 preschool children undergoing elective surgery in our hospital were randomly divided into control group and observation group according to the random number table method,with 90 cases in each group,the control group was given routine nursing,and the observation group with children's fun mode nursing intervention,the curative effect is compared between the two groups.Results Before operation,there was no significant difference between the two groups in the improvement of the perioperative anxiety scale (mYPAS) score in the groups (P ＞ 0.05).During induction of anesthesia,mYPAS score of the two groups before operation were significantly higher than one day before surgery,and the differences were statistically significant (P ＜ 0.05),and anxiety score in the observation group was significantly lower than the control group (P ＜ 0.05).Before one day of operation,there was no significant difference in systolic blood pressure (SBP) and diastolic blood pressure (DBP) between the two groups (P ＞0.05).During induction of anesthesia,the SBP,DBP of two groups were significantly higher than one day before operation,and the differences were statistically significant (P ＜ 0.05),and the observation group had lower SBP and DBP than the control group,the difference was statistically significant (P ＜ 0.05).Before one day of surgery,there was no significant difference in heart rate (HR) between two groups (P ＞ 0.0 5).During induction of anesthesia,the two groups had higher HR than one day before operation (P ＜ 0.05),and the observation group had lower HR than the control group (P ＜ 0.05).The observation group during anesthesia induction had lower cooperation scale (ICC) score than the control group(P ＜ 0.05).Conclusion Nursing intervention of children's fun mode for preschool children can improve children's blood pressure,and has less influence on HR so as to improve cooperation of anesthesia induction.

Objective To evaluate the application of peritoneal shunt in lumbar cistern in the treatment of communicating hydrocephalus.Methods Fifty patients with communicating hydrocephalus were randomly divided into observation group and control group.Patients in control group were treated by ventricle-peritoneal shunt (V-P),and patients in observation group were treated by lumbar cisterna-peritoneal shunt (L-P),the application effect of the two treatment methods for communicating hydrocephalus were compared.Results Compared with V-P shunt,L-P shunt has better clinical effect and lower complication rate.Conclusion The lumbar cisterna shunt has good therapeutic effect in the treatment of traffic hydrocephalus.