Objective:To investigate the biologic roles and potential reverse mechanisms of matrine in human hepatocellular carcinoma cell line QGY and QGY/CDDP.Methods:The MTT assay was used to detect the IC50s of QGY and QGY/CDDP cells,and QGY/CDDP cell were cultivated with matrine.The distinctions of three groups were compared in cell's appearance,growth curve,doubling time,cell cycle,drug-resistance of chemotherapeutics and CDDP contents of intra-cells.Immunocytochemical method was applied to detect the expression of P-gp,MRP and Bax.Results: Matrine could inhibit the growth of QGY and QGY/CDDP cells with no cross drug-resistance.There were some biological differences among QGY,QGY/CDDP and Mat-QGY/CDDP cells.The expressions of P-gp and MRP were decreased;Bax expression was increased,CDDP's toxic effect on QGY/CDDP cells was enhanced,and the drug sensitivity was elevated in Mat-QGY/CDDP cells.Conclusion:Matrine at lower toxic-dose could enhance the toxicity of CDDP on QGY/CDDP cells,and partly effective in reversing the drug-resistance in human multidrug-resistant hepatocellular carcinoma QGY/CDDP cells.

Objective To study the clinical value of esophageal stent placement with radiation therapy for the middle-late pa-tients with esophageal cancer.Methods To perform esophageal stent placement and radiation therapy for the middle-late 35 pa-tients,and to perform radiation therapy for the middle-late 37 patients,then observe the clinical curative effects.Results The treat-ment group were significantly alleviated with dysphagia symptom after operation,which 32 cases sticked the entire radiation,ac-counting for 91.4%,no one can′t complete the treatment because of nutritional status reason.Survival time of one month after ra-diotherapy was up to 100.0%,and survival time of six months was 85.7% (30/35).But the contrast group with dysphagia symp-tom were aggravated or no relief,and needed parenteral nutrition.There were 23 cases to complete the entire radiation,accounting for 62.2%,and 5 cases happened esophageal tracheal fistula.Survival time of one month after radiotherapy was 83.8%(31/37),and survival time of six months was 62.2% (23/37).Two groups of cases in radiotherapy resistance difference was statistically signifi-cant,one month and six months of survival time were statistically significant (all P <0.05 ).Conclusion Esophageal stent place-ment with radiotherapy is more efficient than only radiation therapy to the middle-late patients with esophageal cancer.