Objective To investigate the killing effect by berberine and cytosine deaminase enzyme suicide gene on rectum cancer cells in vitro.Methods Human rectum cancer HR-8348 cells were cultured,then transfected by recombinant adenovirus suicide gene the survivial percentage of which was examined by MTT method.Results Recon of pcDNA3.1(+)-CD was constructed successfully.The CD gene sequence analysis indicated th a+ recon pcDNA3.1(+)-CD contain intact gene.RT-PCR showed CD gene expressed stably in the HR-8348 cells,which is sensitive to 5-FC above the density of 100?g/ml.Elevated along with the medicine density with response time lengthening,its damaging effect enhanced.However for the density above 5-FC 250g/ml,the change of cell killing rate was not apparent.when by 0.1,0.3,3.0,30.0?m density berberine unites the 5-FC on rectum cancer of the intestines Respectively,the cell inhibitory action elevates along with density rises,but 3.0?m,30?m the inhibition change was not apparent.Under 250?g/ml of 5-FC,0.1,0.3,3.0,30.0?m of berberine united the 5-FC The HR-8348 cells growth suppression rate were:27.7%,42.4%,52.3%,56.3% respectively.Conclusion Inhibition function to human rectum cancer HR-8348 cells can be strengthened by berberine combined 5-FC.

Objective To investigate the clinical value of elderly gastric cancer patients undergoing preoperative use of hydroxyethyl amylase. Methods From June 2012 to February 2016 underwent gastrointestinal surgery in our hospital 54 cases of radical resection of gastric cancer patients with clinical research, according to the patients divided into experimental group, control group 27 cases, preoperative intravenous hydroxyethyl starch or Ringer lactate solution, monitoring the hemodynamic changes, inflammatory factors and coagulation indexes in patients in two groups.Results T0 moment, MAP, CVP and HR of experimental group and control group were no significant difference; in T1, T2, T3, MAP and HR of experimental group were higher than that of control group (P<0.05), the CVP value was lower than the control group (P<0.05);T1 T2, T3, PT and APTT in two groups compared with T0 times were significantly increased (P<0.05); pre-treament, there was no significant difference in serum TNF-α, IL-10, IL-6 and CRP between the observation group and the control group; post-treament, The levels of TNF-α, IL-10, IL-6 and CRP in the observation group were lower than those in the control group ( P <0.05 ) .Conclusion The elderly gastric cancer patients undergoing preoperative use of hydroxyethyl amylase is important for inflammation and maintain stable hemodynamics in patients with postoperative .

Objective To construct vector expressing soluble mPDL1-hIgGFc and study its effect on the proliferation and apeptosis of cells in vitro. Methods The extrncellular domain of mPDL1 gene was amplified from pmPDL1 vector by PCR and inserted into phIgGFc vector. The recombinant pmPDL1-hIgGFc was transfected into CHO cells by LipofectAMINETM2000, and the transfected cells were named as CHOp. The expression of mPDL1-hIgGFc in the culture supernatants of CHOp was assayed by ELISA and Western blot. The effects of CHOp culture supernatants on mixed lymphocyte culture(MLC) was analysed by Flowm-etry. Results The extracellular domain of mPDL1 gene were obtained from PCR. DNA sequencing and the identification of digestion by HindⅢ and KpnⅠ indicated the recombinant plasmid pmPDL1-hIgGFc was suc-cessfully constructed. ELISA and Western blot analysis proved that the CHOp could express mPDL1-hIgG-Fc. CHOp culture supernatants could inhibit lymphocyte proliferation and induce the apoptosis of the activa-ted T cells in MLC in vitro in a dose-dependent manner. Conclusion The mPDL1-hIgGFc protein could in-hibit lymphocyte proliferation and induce the apoptosis of the activated T cells.

Objective To evaluate the short -term effect of tegafur combined with oxaliplatin in the treatment of advanced colorectal cancer and its impact on long -term prognosis .Methods 70 patients with advanced colorectal cancer were selected .The patients were randomly divided into tegafur group ( oxaliplatin plus tegafur ) and capecitabine group ( oxaliplatin combined with capecitabine ) according to the digital table ,35 cases in each group , The short-term efficacy,side effects,2-year survival rate and median survival time were compared between the two groups.Results The total effective rate of the tegafur group was 85.71％, which of the capecitabine group was 77.14％,but the difference was not statistically significant between the two groups (χ2 =0.850,P=0.356).After treatment,the levels of VEGF in the two groups were significantly lower than those before treatment ( t =21.694, 20.558,P=0.305,0.249).There was no statistically significant difference between the two groups (t =0.998, 1.242,P=0.281,0.307).The 1-year survival rate was 51.43％and the 2-year survival rate was 25.71％of the tegafur group,compared with 45.71％ and 17.14％ in the capecitabine group ,the differences were not statistically significant (χ2 =0.229,0.764,P=0.632,0.382).The median survival time of the tegafur group was 13.5 months, which of the capecitabine group was 13.0 months,there was no statistically significant difference ( Z=1.304,P=0.752).The incidence rate of hand -foot syndrome of the tegafur group was 5.71％,which was lower than 22.86％of the capecitabine group (χ2 =4.163,P=0.027).Conclusion The efficacy of tegafur combined with oxaliplatin in the treatment of advanced colorectal cancer is similar to capecitabine plus oxaliplatin , with a slightly lower complication rate.

Objective To evaluate the experience of laparoscopic complete mesocolic excision (CME) for right colon cancer.Methods 45 patients with right colon cancer underwent laparoscopic CME were selected as research subjects(laparoscopy group),and 40 patients with right colon cancer underwent open CME were selected as control group(open group).The clinical effects of the two groups were observed .Results All the 45 cases were successfully performed with laparoscopic CME and 40 specimens were evaluated pathologically as mesocolic plane surgery. The average time for passage of flatus in the laparoscopy group [(3.43 ±0.72)d] was less than that in the open group [(4.10 ±0.99)d,P =0.039,P <0.05].The average time of leaving bed [(3.39 ±0.66)d] in the laparoscopy group was significantly less than that in the open group [(4.20 ±0.79)d,P =0.005,P <0.05].The mean operative time of the laparoscopy group [(160.93 ±20.91)min] was longer than that of the open group [(103.00 ±24.29)min,P =0.000,P <0.05].The average number of total lymph nodes removed of the laparoscopy group [(23.16 ±8.21)] was higher than that of the open group [(19.06 ±7.48)],but the difference was not statistically significant between the two groups (P =0.102,P >0.05).The overall postoperative complication rate of the laparoscopy group was 13.33%,which was lower than 22.50% of the open group,but the difference was not statistically significant between the two groups(P =0.268,P >0.05).Conclusion Laparoscope complete mesocolic excision for right colon cancer is safe and feasible.

Objective To investigate the changes of T lymphocyte subsets and quality of life after administrating Aidi injection in patients with colon cancer. Methods A total of 86 patients with colon cancer admitted to our hospital from February 2015 to May 2017 were enrolled in this study. The patients were divided into chemotherapy group (43 patients, conventional therapy) and combination group (43 cases,conventional chemotherapy+Aidi injection) by random number table. Compared the recent curative effect, level of T lymphocyte subsets, changes of quality of life and adverse reactions before and after treatment. Results There were significant differences in the distribution of CR, PR, SD and PD between the combination group and the chemotherapy group(P＜0. 05). The former had a clinical benefit rate of 72. 09％ and the latter was 51. 16％. The difference was statistically significant(P＜0. 05). There was no significant difference in the level of T lymphocyte subsets and quality of life in the combination group after treatment(P＞0. 05). The CD3+, CD4+, CD4+/CD8+ and scores of quality of life in the chemotherapy group were significantly lower(P＜0. 05) after treatment, CD8+ increased in both groups, and there was statistical difference between the combination group and the chemotherapy group after treatment(P＜0. 05). The incidence of adverse reactions in the combination group was lower than that in the chemotherapy group(P＜0. 05) and the difference of classification comparison was also statistically significant(P＜0. 05). Conclusion The use of Aidi injection in colon cancer patients with chemotherapy can improve the short-term curative effect, avoid the damage of immune function and reduction of the quality of life, and relieve and reduce the adverse reactions caused by chemotherapy.

Objective To investigate the difference on long-term effect of laparoscopy and laparotomy operation in patients with colon cancer. Methods Medical records of 150 patient who nderwent radical operation of colon cancer by the same team of surgeons in our hospital from January, 2010 to January, 2013 were randomly selected and reviewed retrospectively. Patients were divided into laparoscopy operation group (77 patients) and laparotomy operation group (73 patients) according to the type of operation. Rates of 2-year and 5-year survival, the incidence of local recurrence and distant metastasis as well as the incidence of complications during follow-up period (6 months to 5 years). Results Rates, of 2-year and 5-year survival in laparoscopy operation group were 79. 22％ and 31. 17％ respectively. Rates of 2-year and 5 -year survival in laparotomy operation group were 79. 45％ and 30. 14％ respectively. There were no statistical differences between two groups (P＞0. 05). The incidence of recurrence and metastasis in laparoscopy operation group was 20. 78％, which was not statistically different from that in laparotomy operation group (17. 81％) (P＞0. 05). The incidence of complications during follow-up period in laparoscopy operation group was 5. 19％, which was statistically different from that in laparotomy operation group(17. 81％)(P＜0. 05). Conclusion There was no significant difference between laparoscopy and laparotomy operation in patients with colon cancer. However, there were fewer complications after laparoscopy oper- ation. Laparoscopy operation enjoyed advantages over laparotomy operation and it was worth further study and promotion.