Objective:To eliminate the interference of matrix in urea cream on the microbial limit test. Methods: A certain a-mount of magnesium sulfate was added to the test solution followed by stirring and placing sometime to make the cream layering, and the water layer was used for the microbial limit detection. Results:After magnesium sulfate was added to urea cream, microbial limit test was not affected by the matrix. The average recovery of Escherichia oli, Staphylococcus aureus, Bacillus subtilis, Candida albicans and Aspergillus niger was 96. 00%, 97. 00%, 100. 00%, 99. 00% and 101. 00%, respectively. The negative and positive results of Psudomonas aeruginosa and Staphylococcus auress were reliable. Conclusion:In the detection of microbial limit for urea cream, mag-nesium sulfate can be used as a good auxiliary agent to make the detection method more convenient and reliable.

Objective:To explore the dynamic changes of nitric oxide and prostaglandine levels in gastric mucosa and plasma of rats with seawater immersion injury after open abdominal trauma Methods:Thirty-two Sprage-Dawley rats were randomly assigned into four groups:normal control group,celiac seawater-immersing trauma stress 1,2,3h groups.The contents of nitric oxide and prostaglandine in gastric mucosa and plasma of rats were detected by chemochromometric and immunoassay methods respectively.Results:Compared with normal control group,both the nitric oxide and prostaglandine levels in gastric mucosa and plasma of rats under celiac seawater-immersing trauma were progressively increased (P

The connective tissue growth factor is a member of an emerging gene family known as the CCN family, which is an exocrine-type cysteine-rich polypeptide, and i t is also the downstream effector of the transforming growth factor? 1 and mediates the growth and migration of the vascular smooth muscular cell and the endothelium. The connective tissue growth factor plays an important role in the angiogenesis during wound repair, but its mechanism in tumor angiogenesis is sti ll poorly understood. The breast is abundant in connective tissue matrix, especi ally higher in breast carcinoma, so the connective tissue growth factor probably plays an important role throughout the biological course of breast carcinoma.

The Thomsen Friedenreich(TF) related antigen is a group of tumor related carbohydrate epitopes expressed in several kinds of tumors, and it is closely related with the biological behavior and prognosis of tumor. TF antigen also has great significance in the diagnosis and therapy of tumors. This article reviewed the relation between TF antigen and several kinds of malignant tumors such as the tumor in digestive system, pulmonary carcinoma and mastocarcinoma.The diagnostic, therapeutic and prognostic value of the TF antigen is also primarily discussed.

Objective The aim of this study was to investigate the relationship of gastric mucosal lesions and the parietal oxyntic function in rats under stress as a result of seawater-immersion after open abdominal injury. Methods Thirty-two SD rats were subjected to open abdominal injury followed by seawater immersion. The animals were randomly divided into four groups: control, 1h, 2h, 3h stress for 1h, 2h, and 3h groups. The pH value of gastric juice and gastric mucosal ulcer index (UI) were measured. The gastric mucosal lesions were observed with light microscopy, and the ultrastructural changes in parietal cells were observed by transmission electron microscopy. Results There was a significantly negative relationship between UI and pH value (r=-0.70,P

Objective To investigate the diagnostic and treatment methods of appendiceal mucoceles and peritoneal pseudomyxoma. Methods Thirteen cases of appendiceal mucoceles were enrolled in this study. Six cases underwent appendectomy, two cases ileocecal resection, two cases right colectomy, and two cases appendectomy in emergency followed by right colectomy because their postoperative pathological diagnosis was appendiceal muco-cystadenocarcinoma. Ruptured appendiceal mucocele and peritoneal pseudomyxoma were found during operation in one case, then right colectomy was performed, ascites was removed and abdominal cavity was washed with normal saline and 5-Fu solution. Systemic chemotherapy was given to this patient after operation. Results Operation was successful in all the patients. Except one case not followed up, the other patients remained free of disease after operation. Conclusion Appendiceal mucoceles is uncommon. The patients should be suspected suffering from this disease when chief complaints are right lower abdominal pain and mass. B-type ultrasonography and other necessary digestive image examinations are economical and inexpensive diagnostic method of appendiceal mucoceles. It is necessary to explore the whole abdominal cavity carefully when this disease is suspected during operation.

AIM:To observe the dynamic changes of neurokinin A(NKA) and calcitonin gene-related peptide(CGRP) levels in gastric mucosal and plasma in rats after abdominal seawater-immersing trauma,and to investigate the influence of these two sensory neuropeptides on acute gastric mucosal lesion.METHODS:Thirty-two SD rats were randomly divided into four groups(normal group,celiac seawater-immersing trauma 1,2 and 3 h groups).With emzyoimmunoassay and radioimmunoassay respectively,gastric mucosal and plasma NKA and CGRP levels in rats were measured.RESULTS:Compared with normal rats,with the seawater-immersing time prolonged,gastric mucosal NKA and CGRP levels in rats were progressively decreased(P

Objective:To explore the clinicopathologic features of 112 patients with mantle cell lymphoma (MCL). Methods:Da-ta from 112 MCL cases were collected, and immunohistochemical assay was conducted. A break in the CCND1 gene was detected by fluorescence in situ hybridization (FISH). The t-test was used in the statistical analysis. Results:All tumor cells in the 112 cases ex-pressed B cell-related antigen, including 1 blastoid subtype and 1 polymorphic subtype. Among all the cases, 106 expressed CD5 and 104 expressed cyclinD1. A break in the CCND1 gene was not found in 3 cases with CD5-MCL. IgH/CCND1 polyploid was found in 2 classical cases. Conclusion:MCL is a type of special immunophenotypic B-cell lymphoma. The prognoses of blastoid and polymorphic subtypes are poor. Special subtypes should be classified during diagnosis.

Objective To investigate the cause and management of postsplenectomy fever in portal hypertensive patients . Methods The clinical data of 295 portal hypertension patients undergoing splenectomy from 1990 to 2003 were reviewed. Among these,80 patients suffered from a continuous fever higher than 38.5℃ for more than 2 weeks postoperatively. Results Except for two patients with unknown cause, 78 of 295 patients with continuous fever were caused by complications such as splenoportal thrombosis(35 cases), infection of hematocele or hydrops in splenic recess(20 cases), left subphrenic infection(7 cases), pneumonia and hydrothorax or empyema(5 cases), 3 cases each of postoperative abscess of tail of pancreas,winary tract infection and inteclion of surgical incision, 1 case of leakage of esophageal anastomosis and intraabdominal infection in 1 case. The lasting fever was related to the grade of liver function(P0.05). Conclusions Splenoportal thrombosis, and hematocele, hydrops or infection in the splenic recess were the main causes of persistent fever after splenectomy. Prevention and treatment of infection and amelioration of hepatic function will help to reduce the rate of postoperative continuous fever.

Objective To explore the characteristics of clinical symptoms and serum brain-derived neurotrophic factor(BDNF)level between depressive patients with and without attempted suicide behavior.Methods Serum BDNF level in depressive patients with(n=36)and without(n=55)attempted suicide behavior were assayed by ELISA,the severity of depression was measured by Hamilton rating scale for depression(HAMD).Results HAMD24 total scores(t=3.632,P=0.000),cognitive disturbance(t=-2.339,P=0.019)and hopelessness factor scores(t=-2.812,P=0.005)in depressive patients with attempted suicide behavior were significantly higher than those in depressive patients without attempted suicide behavior.There were no significant differences of anxiety/somatization,body weight,diurnal variation,psychomotric inhibition and sleeping disturbance fator scores between two groups(P＞0.05).The serum BDNF level in depressive patients with attempted suicide behavior was significantly lower than that in depressive patients without attempted suicide behavior(t=-2.122,P=0.037).There was no significant difference of serum BDNF level between male and female patients(P＞0.05).Conclusion There were certain characteristics on clinical symptoms of depressive patients with attempted suicide behavior.The low serum BDNF level might play an important role in the risk of suicide in depressive patients.