Objective To observe the effects of ultrashort wave diathermy on the expression of tumor necrosis factor-α (TNF-α) andBcl-2 in hippocampus, striatum and motor cortex of rats with cerebral ischemia/reperfusion. Methods The model of focal ischemia/reperfusionin Sprague-Dawley rats was induced with intraluminal middle cerebral artery occlusion (MCAO) with nylon monofilament suture. Therats were divided into 3 groups: sham group (n=6), model group (n=6) and ultrashort wave diathermy group (n=6). Brain tissue slices wereimmunohistochemically stained (SABC) to observe the expression of TNF-α and Bcl-2. Results The expression of TNF-α and Bcl-2 in lefthippocampus, striatum and motor cortex was higher in the model group than in the sham group (P<0.01). The expression of TNF-α was lowerbut Bcl-2 was higher in the ultrashort wave diathermy group than in the model group (P<0.05). Conclusion Ultrashort wave diathermycan affect the expression of TNF-α and Bcl-2, which may associate to the neuroprotection from focal cerebral ischemia/reperfusion injury.

Objective To investigate the effect of vertical bed training on the extubation time and pulmonary infection of patients with tracheotomy in rehabilitation department .Methods Sixty-five new patients with tracheotomy and pulmonary infection more than a month were randomly divided into the control group (33 cases) and the observation group (32 cases) by stratified block randomization .All the patients received routine nursing and rehabilitation therapy , in addition, the patients in the observation group received electrodynamic vertical bed training .The decannulation rate , cure rate of pulmonary infection , sputum culture negative rate and the clinical pulmonary infection scale ( CPIS) were compared.Results One patient was lost in each of the groups during follow up .After the nursing intervention , the CPIS of the observation group was significantly lower than that in the control group (t=9.276,6.609, respectively;P<0.01).22 patients (71.0%) in the control group removed the tracheal tube;20 patients (64.5%) cured of pulmonary infection;15 patients (48.4%) had negative sputum culture;the sare of CPIS was (2.8 ±1.1), those values of the control group were 43.8%, 37.5%, 25.0%and (3.9 ±1.5).There were significant differences between groups ( t/χ2 =4.76,4.60, 6.22,3.36, respectively;P<0.05).Conclusions Vertical bed training can reduce the time of tracheostomy , and help in control the pulmonary infection of patients with tracheotomy in rehabilitation department .