Objective To study on 24 hours hernia grassroots level in the district hospital management model practices. Methods The subjects were 283 cases of inguinal hernia patients selected from January 2008 to December 2010 in our hospital as the traditional model of the group , the treatment in the traditional management model , our hospital im-proved the management mode after January 2011 , 326 cases of inguinal hernia patients selected from January 2011 to December 2013 in our hospital as a new model group , that under the new model in 24 hours hernia treatment mode. Results Under the new model,patients waiting for surgery, hospitalization time and the days of antibiotic use were sig-nificantly shorten than the traditional model , the cost of hospitalization was significantly reduced , the difference was statistically significant (P<0.05). Under the new model, patient satisfaction was significantly higher than the traditional model, and the complication rate was significantly lower than the traditional model, the difference was statistically sig-nificant (P<0.05). Conclusion 24 h hernia is safe, effective, and economic characteristics, enabling a more rational use of health care resources and deployment , significantly reducing health care costs , can promote the application in district hospitals.

OBJECTIVETo identify a rare transcription mutation (C-->T) at position -90 of the beta-globin gene previously unreported in the beta-thalassemia carriers from a Chinese family.

METHODSIn phenotype analysis, standard hematological techniques were used to measure RBC counts and Hb concentration. Reverse dot blot (RDB) analysis, which can simultaneously detect 18 known types of beta-thalassemia mutations in Chinese, was used to scan beta-globin gene mutations. DNA sequence analysis of the entire human beta-globin gene was performed to characterize the underlying causative mutation of the sample and to identify its genotype. A semi-quantitative RT-PCR method was used to measure beta-globin gene expression in the form of mRNA from the subjects.

RESULTSThe proband, his brother and his mother presented a typical beta-thalassemic trait with reduced mean corpuscular volume (MCV, 68.2-73.6 fL) and elevated level of Hb A(2) (5.7%-6.4%) but no known beta-thalassemia mutations were found in the samples by RDB analysis. DNA sequencing of the beta-gene region of these three samples revealed heterozygosity for the C-->T substitution at position -90 within proximal CACCC box of the beta-globin gene promoter element, which was previously unreported in the Chinese population. Analysis of mRNA from the positive carriers demonstrated that the mutant beta-globin gene significantly reduced beta-globin transcription (mutants: 2.233 +/- 0.01 vs normal: 3.779+/-1.19; 95%CI: 3.060, 4.499), showing a level comparable with that of the other beta-thalassemia heterozygotes (2.110+/-0.53, 95%CI: 1.732, 2.488).

CONCLUSIONA rare transcriptional mutation that led to beta-thalassemia in Chinese population has been characterized. The findings enrich knowledge of the mutation spectrum of beta-thalassemia.

Adult ; Female ; Globins ; genetics ; Humans ; Mutation ; Transcription, Genetic ; beta-Thalassemia ; genetics

OBJECTIVETo investigate the effects of oral contraceptive pretreatment (OCP) in patients with polycystic ovarian syndrome (PCOS) undergoing controlled ovarian hyperstimulation for IVF-ET.

METHODSWe randomly divided 85 patients with P-COS undergoing IVF-ET into an OCP (n = 53) and a control group (n = 32), the former received OCP, while the latter did not before the cycle. We retrospectively analyzed the data of the patients for the ovulation promoting effect of OCP and its influence on the incidence of ovarian hyperstimulation syndrome (OHSS) and outcomes of IVF-ET.

RESULTSCompared with the control group, OCP significantly reduced the formation of ovarian cyst (P < 0.05), remarkably increased the duration of gonadotropin stimulation and consumption (P < 0.01) , and markedly raised the percentage of mature ova (87.92% vs 92.85%, P < 0.05). But no significant differences were observed between the two groups in the incidences of moderate and severe OHSS, number of retrieved oocytes, and rates of fertilization, miscarriage and clinical pregnancy.

CONCLUSIONOCP for patients with PCOS can help to control the time of ovarian stimulation, improve the synchronism of follicular development, and increase the duration of gonadotropin stimulation and consumption, but cannot change the incidences of moderate and severe OHSS.

Adult ; Contraceptives, Oral ; therapeutic use ; Embryo Transfer ; methods ; Female ; Fertilization in Vitro ; methods ; Humans ; Infertility, Female ; etiology ; therapy ; Ovulation ; Ovulation Induction ; Polycystic Ovary Syndrome ; complications ; Pregnancy ; Pregnancy Outcome ; Retrospective Studies