Objective:To compare the effects of different surgical treatment of chronic rhinitis-sinusitis difference,and its impact on transport function of maxillary sinus mucociliary,provide a clinical-surgical sinusitis preferably reference about the treatment of chronic nasal formulation for.Methods:From 2013.9-2014.12,otorhinolaryngology clinic in our hospital,160 cases were diagnosed as chronic nose-as research subjects were randomly divided into four groups of patients with sinusitis were treated from 1 to 4,60 cases in each group;treatment groupl receiving the maxillary sinus ostium augmentation,treatment group 2 received fenestration,treatment group 3 through tears crypt before maxillary sinus surgery,treatment 4 group receiving the maxillary sinus balloon dilatation;after covering the nasal mucosa observed and compared four groups of patients about edema,vesicle formation,bone exposure,scarring,etc.,as well as internal maxillary sinus secretions traits,volume situation,and compared patients after four groups line maxillary sinus cavity and perioral saccharin test biopsy cases three months and six months.Results:The four groups were made after surgery better clinical efficacy,and group efficacy 4 with the other three groups,the effect is most significant (all P ＜0.05),the difference was statistically significant,and after three months and six months,group 4 compared with other surgical group,the Lund-Kernedy score were also lower,MMT time were also lower,the difference was more significant (all P ＜0.05);in the maxillary sinus biopsy aspects:the number of postoperative inflammatory cells and dendritic cells and glandular cell morphology and submucosal edema improved submucosal structures also in the group of four most significant (P＜0.05).Conclusion:Maxillary sinus balloon dilatation treatment chronic nasal sinusitis curative effect is higher,which can effectively improve the cell and submucosal gland cell morphology and sinus mucosa edema,sinus unobstructed drainage effect,and high safety.

Objective　To investigate the effect of positive pressure ventilation on radiosensitivity of the patients suffering from primary lung cancer. Methods　Thirty cases of lung cancer were randomly divided into two groups: ① combining therapy group: patients treated with posi tive pressure ventilation using BiPAP respirator and radiotherapy;② simple radi otherapy group. The changes of PaO2, SaO2, blood white cells and the cellula r immunological function were observed before and after treatment. Resul ts　Nasal or naso-facial positive pressure ventilation with BiPAP respi rator increased PaO2 significantly with the maximum of 7.5 kPa, SaO2 was ma inteined at above 95%. No significant change for the cellular immunological func tion was found in the combining therapy group （P>0.05）and only one patient with leukopenia（6.7%）. But in the radiotherapy group, the lymphocyte transfor mation efficiency and the ratio of CD4/CD8 were obviously decreased（P<0 .01), and leukopenia was found in 5 cases（33.3%）. Conclusion　 Nasal or naso-facial positive pressure ventilation with BiPAP respirator could improve the effect of radiotherapy for lung cancers. It could also increase PaO 2 significantly and has protective rote to the marrow and cellular immunologic al function of the patients.

Adipose Tissue ; Animal Feed ; Animals ; Hypothalamus ; drug effects ; physiology ; Leptin ; pharmacology ; Male ; Mice ; Mice, Inbred Strains ; RNA ; analysis

OBJECTIVE:To prepare ambroxol hydrochloride orally disintegrating tablets and to evaluate its quality.METHODS:Mannitol was used as main adjuvant to prepare ambroxol hydrochloride orally disintegrating tablet,the UV spec-trometry was adopted to determine ambroxol content,the disintegration time,taste and dissolution rate were evaluated.RESULTS:The optimal formulation was composed of MCC 50g,mannitol 20g,L-HPC 10g,and NaHCO3 2g;The detected concentration of ambroxol was linear in the range of 0.005～0.030mg/ml(r=0.9 999),the average recovery was 100.50%and RSD=0.71;The prepared tablets disintegrated completely within 30s.CONCLUSION:The orally disintegrating tablets is quick to disintegrate and is as effective as market tablets.

Objective To observe the effects of Sancaoanqian decoction on expression of cytokine IL-1?, IL-6 and TNF RⅡ in experimental autoimmune prostatitis rats, and explore the target protein of cytokine of Chinese herbal compound in treating chronic nonbacterial prostatitis. Methods Seventy-two male Wistar rats were divided randomly into six groups and 12 rats in each group. All groups were made experimental autoimmune prostatitis rat model by injecting SC purified prostate protein with FCA except normal group. The normal and model group were given saline, western medicine group was given indomethacin, large, medium and small doses decoction groups were treated with large, medium and small doses of Sancaoanqian decoction. All rats were sacrificed after 30 days. The mRNA and protein expression of cytokines were tested by methods of immunohistochemistry and fluorescent quantitation RT-PCR. Results Sancaoanqian decoction could reduce the mRNA and protein expression of IL-1?, IL-6 in experimental autoimmune prostatitis rats. There were no significant difference between large and medium doses decoction group, but had a better activity than small dose. The expression of TNF RⅡ were reduced in all doses groups and no difference among three doses of Sancaoanqian decoction. Conclusion Sancaoanqian decoction can regulate the immune function in chronic prostatitis rat model. Down-regulation of cytokines maybe one of important mechanisms in treating chronic prostatitis.

Objective To study P300 wave extraction by a new method. Methods According to the respective characteristics of the brain electrical signal and the signal analysis method, the algorithm by Wavelet Transformation (WT) combined with Independent Component Analysis (ICA), abbreviated to WICA, was applied to P300 wave extraction. Results With WT, the target component of the signal could be strengthened, while the non-target component and the noise were weakened which became weak in multi -channel ICA input signal, and then WICA algorithm could effectively separate strong target component. Conclusion WICA algorithm behaves well in P300 wave extraction.

OBJECTIVE To investigate the present situation of hand-washing among medical staff then explore more suitable hand-washing methods and monitoring measure. METHODS By surveying the hand-washing situation of 96 medical staff in our hospital through the method of sampling,the different germ eradication rate between routine soap hand-washing and disinfectant wet towel hand-wiping as well as hand-washing rate in all candidate population during practical work were observed. RESULTS The germ eradication rate after routine soap hand-washing was 86.0%,while by disinfectant wet towel wiping it was increased to 91.3%.The washing rate after above former method was 18.3% and after above two methods combined was increased to 43.6%,and after health propaganding and training was up to 60.6%. CONCLUSIONS Disinfectant wet towel hand-wiping is convenient,practical and effective which would be more suitable for clinical practice when combined with routine soap washing method.

Objective To improve the cure rate of hepatobiliary lithiasis.Methods Two hundred and seventy-five patients with retained gallstones in the hepatic bile ducts after choledochostomy were treated with choledochofiberscope from August 1989 to August 2001 in this hospital.Results In 252 cases(91.6%), the residual gallstones were thoroughly removed with choledochoscope and in remaining 23 cases(8.4%), the stones were not completely removed. The factors affecting the results of the procedures include improper locating of T-tube, the exfoliation of T-tube and closure of fistula, the size and the incarceration of retained gallstones, the narrowed bile ducts, the performance of intraoperative choledochoscope, the method of operation.Conclusions Choledochofiberscope is of great value in surgical treatment of hepatobiliary lithiasis. However it can't completely replace to remove the damaged liver caused by the gallstones.

With the rapid development of pharmacogenetics, more and more studies have shown evidence in the association between polymorphisms at the human leukocyte antigen (HLA) loci and severe adverse drug reactions (SADRs). Several HLA-B alleles proved to be associated with SADRs for drugs such as carbamazepine, allopurinol, lamotrigine, and lfucloxacillin. hTe USA Food and Drug Administration (FDA) has even recommended routine screening for HLA-B allele before the use of abacavir and carbamazepine. With the completion of human genome project and the Hapmapproject, several new pharmacogenetics approaches such as genome-wide association study (GWAS) have emerged. hTese newly developed methods will undoubtedly accelerate the identiifcation and clinical utilization of the pharmacogenetic biomakers. In addition, the immunogenetic mechanisms by which the HLA alleles cause SADRs are explored at the cellular and molecular level. hTis review focuses on the recent progresses in HLA alleles and ADRs regarding both the clinical translation and modern pharmacogenetic methods.

Objective To study the effect of glutathione(GSH)on liver antioxidative function of microcystin‐LR(MC‐LR)‐in‐duced mice .Methods Forty healthy clean class KM 5‐week old mice were selected and divided into five groups by the random sam‐pling method ,including the norml saline control group ,GSH control group ,MC‐LR group ,MC‐LR+low dose GSH group and MC‐LR+high dose GSH group ,8 cases in each group ,half male and half female .The experiment lasted for 15 d by intraperitoneal injec‐tion of MC‐LR ,then the liver histopathological changes ,liver tissue activity of superoxide dismutase (SOD) ,glutathione peroxidase (GSH‐Px)and content of malondialdehyde(MDA) were detected .Results Compared with the normal saline control group ,liver cell GSH level ,SOD and GSH‐Px activities in the MC‐LR group were significantly decreased (P<0 .05) ,while the MDA level was sig‐nificantly increased (P<0 .05) .Compared with the MC‐LR group ,the GSH level ,SOD and GSH‐Px activities in the MC‐LR+low dose GSH group and MC‐LR+high dose GSH group were significantly increased (P<0 .05) ,while the MDA level was significant‐ly decreased(PP<0 .05) .Conclusion The GSH intervention can alleviate MC‐LR induced mouse liver oxidative toxicity and has protective effect on the liver to some extent .