Objective To explore the role of intracellular Ca~(2+) in inhibition of axonal outgrowth,which could learn more about the signal transduction mechanism of Nogo-A inhibiting axonal outgrowth. Methods Using laser confocal microscope,intracellular calcium level in cerebellar granule neurons after Nogo-A added in different times was measured and the effect of Nogo-A inhibiting axonal outgrowth when priming nimodipine was observed. Results The concentration of Ca~(2+) increased in 5 minutes after Nogo-A added,and reached to peak in 30 minutes,then decreased gradually,90 minutes later dropped to normal level.When priming with nimodipine,the axonal inhibition by Nogo-A was partly abrogated compared with control.Conclusion Ca~(2+) may participate in the signal transduction of Nogo-A inhibiting axonal outgrowth.

Objective:To study the quality standard for Fujie lotion. Methods:The qualitative identification of Sophora alopecu-roides L, Cnidium monnieri ( L. ) Cuss and borneol was detected by TLC. The quantitative determination of matrine was detected by HPLC. Results:The identification by TLC was highly specific, and the content determination method was accurate and repeatable. The linear range of matrine was 0. 013 0-1. 30 mg·ml-1, and the average recovery was 97. 1% with RSD of 1. 7%(n=6). Conclu-sion:The standard can effectively control the quality of Fujie lotion.

Objective To evaluate the possibility, methods and efficiency of simultaneous transcatheter therapy for perimembranous ventricular septal defect(PMVSD) combined with atrial septal defect(ASD).Methods Four patients with PMVSD combined with ASD, including 3 males and 1 female, age ranging from 12 to 26 years; underwent simultaneous attempted transcatheter therapy. The diameters of PMVSD were 3-6 mm and the distances from the defect rim to aortic valve were 2-6 mm by the echocardiography before the precedure. The stretched diameter of ASD was 6-10 mm. PMVSD were occluded using homemade two-disc PMVSD occluder first and the ASD were occluded later on. Results All patients were treated successfully at one time. The diameter of PMVSD were 3.5-10.0 mm, the diameters of occluder was 4-12 mm. The stretched diameter of ASD was 6-10 mm and the occluder diameter was 6-12 mm. No residual shunt was found by the transthoracic echocardiography and left ventriculography after the occluders deployed. No complication occurred. Conclusion Simultaneous transcatheter therapy for PMVSD combined with ASD is feasible, safe and effective.

Objective To evaluate the feasibility and advantage of using withdrawable Sigma stent for the treatment of tracheo-esophageal fistular. Methods The stents were placed into trachea or/and esophagus by interventional or/and endoscopic technique. Results Esophageal cancerin 17 and benign disease in 2. Totally 38 stents were placed in 19 cases of patients (trachea 19, main bronchus 1, esophagus 18). Only one tracheal stent was placed in 2 cases. Two stents(one in trachea, another in esophagus) were placed in 15 cases ( 11 cases with 2 tubular type stent, 4 cases with one tubular and one bifurcated type stent). Three stents were placed in 2 cases. One stage placement of the stent in 35, withdrawed the stent and reinserted again in 3. All the patients have normal meal 2～4 days postoperatively. Only one patient had a little contrast in the trachea during X-ray exam but without symptoms, the fistulae completely sealed in 18 cases. Follow-up was fron 3 months to 3 year. 10 patients were still alive; the longest survival is 18 months. There were 9 deaths. The causes of death were pulmonary infection in 1, hemorrhage in 1, and systemic metastasis in 7 cases. Conclusion Sigma stent is can effectively treat tracheal or/and esophageal stenosis or fistular.

Objective To investigate the methods and therapeutic effects of anterolateral thigh perforator free flaps for reconstruction of soft tissue defects around the ankle.Methods A retrospective case series study was made on 30 patients treated with anterolateral thigh perforator free flaps for traumatic soft tissue defects around the tibia between January 2010 and June 2016.There were 22 males and eight females,with the age range of 16-58 years [(41.5 ± 1.2) years].Dimension of the soft tissue defect ranged from 9 cm ×4 cm to 23 cm × 12 cm.Flap stabilization time,flap survival rate,postoperative complications,second plastic surgery rate,and ankle joint function by Baird-Jackson score were recorded.Results All patients were followed up for (13.0 ± 5.4) months (range,6-24 months).Time for flap stabilization was (6.5 ± 1.1) days.All flaps survived except in one patients with flap loss due to postoperative arterial thrombosis.Postoperatively,one patient had arterial crisis,one had venous thrombosis,and one presented sinus formation due to infection.No second plastic surgery was carried out.According to the Baird-Jackson score,the results were excellent in 25 patients and good in five.Conclusion Anterolateral thigh perforator free flaps for reconstruction of traumatic soft tissue defects around the tibia has advantages of easy harvesting and survival,less postoperative complications,less second surgery,and good function recovery.

Objective:To study the effects of supranutrional levels of yeast-selenium on antioxida-tion status and immune function in mice with tumour.Method:Sixty KM mice were divided into three groups: control group,yeast-selenium Ⅰ group and Ⅱgroup.The mice were supplemented with 0.5ml normal saline,3.2 ?g and 6.4 ?g yeast-selenium respectively by gavage on time a day based on the low selenium diet.The mice were inoculated with Ehrich ascites carcinoma(EAC) after 13 d.The tumour weight and immune function and antioxidative indices in blood were measured after 23 d.Results: The two yeast-selenium levels increased the activities of GSHPx(P

Objective: To study the efficacy of purging the lung and removing phlegm and blood stasis method(泻肺化痰祛瘀法) on animal model of pulmonary hypertension induced by monocrotaline and explore its mechanism.Methods: The model of pulmonary hypertension induced by monocrotaline was established in rats.Sixty healthy and clean male Wistar rats were randomly divided into normal control group,model group,low and high dosage of traditional Chinese medicine(TCM) drugs for purging the lung and removing phlegm and blood stasis groups.From the 15 th day after model establishment,two dosage groups were treated with the drugs in the prescription containing the main ingredients as follows: 9 g prepared rhubarb(制大黄),(2 g)(prepared)(euphorbia) kansui(炙甘遂),15 g lepidium apetalum(葶苈子),9 g ligusticum(川芎) and 15 g(scutellaria)(黄芩) infused into the stomach once a day for 7 days.Normal control group and model group were given normal saline infused into the stomach.Mean right ventricular pressure(MRVP) and the mean(pulmonary) artery pressure(MPAP) of rats were measured by right cardiac catheterization,and the(pathological)(changes) of lung tissues were detected by hematoxylin and eosin(HE) stained sections.(Results:) The MRVP and the MPAP in high dosage group((13.37?4.22)mm Hg and(12.26?2.79) mm Hg,(1 mm Hg)=(0.133 kPa)) were lower than that in the model group((16.27?2.27) mm Hg and(14.77?(2.39) mm Hg),P

Objective Normal radiographic scaphoid lunate (SL) interval is thought to be 2 mm and abnormal SL interval is a prerequisite for diagnosing scapholunate instability. However, in the routine neutral rotation posterior anterior (PA) view of the wrist, the frequent overlapping between scaphoid and lunate precludes proper interpretation of the interval. Moreover, the normative data in Chinese population has not been reported. We developed a new but simple technique in radiological assessment to evaluate the normal SL interval in Chinese. Methods With the forearm in neutral rotation, a PA view of the wrist was taken using a mobile Xiscan. A radiolucent cushion was put underneath the hypothenar eminence so that the metacarpal arch of hand became parallel to the receiver table of the Xiscan. The normal supination posture of the wrist was thus corrected and the true SL interval was revealed. Two hundred normal wrists were examined; there were 46 female and 54 male healthy Chinese subjects, with age ranging from 18 to 80. The SL interval was determined with wrist in neutral, radially deviated and ulnarly deviated position using the cushioned neutral rotation PA view. The length of capitate, hamate and lunate were also measured to correlate with the SL interval. Results By using the standard PA view, the SL interval could not be determined in 60% of cases. Using the new assessment method, the optimal intervals were seen in all cases. The average SL interval was (2.07?0.65) mm. There was no significant gender difference. The measurement of ulnar variance was not affected by the new method. Conclusion The modified (Cushioned) PA view was the optimal radiological assessment method for true SL interval. There was wide variation of the normal value of the SL interval in Chinese population.

ObjectiveTo investigate the mechanism by which tetracycline inhibits the development of experimental abdominal aortic aneurysms (AAA).MethodsMale Wistar rats underwent intraluminal perfusion of the abdominal aorta with 50 units of porcine pancreatic elastase followed by treatment for 14 days with tetracycline (25?mg/day, subcutaneously; n=10) or with NS alone (n=10). Aortic wall elastin and collagen concentrations were measured with image analysis system and fixed aortic tissues were examined by light microscopy for inflammation. The expression of MMP-2 and MMP-9 were observed in aortic tissues using immunohistochemistry and molecular in situ histohybridization technique. Results AAAs developed in all NS-treated rats, and only 1/10 of tetracycline-treated rats at 14 days(P

Objective To investigate p53 protein expression in nasopharyngeal carcinoma (NPC) and its correlation with biological behavior, p53 gene mutation and EBV infection in 43 cases of NPC. Methods Immunohistochemical staining was applied to detect p53 protein,PCR-SSCP was used to detect p53 gene mutation, and EBV infection was determined with PCR. Results The positive rate of p53 protein expression was 76 7%(33/43)in the NPC. The positive rate of p53 protein expression in stages Ⅲ and Ⅳ was significantly higher (80 6%) than that in stages Ⅰ and Ⅱ(66 6%) (P