Aim To explore the mechanisms of Curcumin which plays a role of protecting cells through regulating the activity of GSK-3?.Methods Plasmid APPswe and BACE1-mychis were transiently co-transfected into SHSY5Y cells.The cells were treated with Curcumin and then were collected to determine the mRNA and protein levels of GSK-3?,?-catenin and CyclinD1 by RT-PCR and Western blot. Immuno-fluoresecnt staining was used to detect the location and expression of ?-catenin and GSK-3?, and ELISA was for the generation of A?.Results RT-PCR and Western blot results showed that in the cells, Curcumin inhibited the expression of GSK-3? mRNA and protein, and promoted the expression of GSK-3?-Ser9 protein (P

On the basis of the origin,development and modern research of xuanfu theory,a new view of nose xuanfu theory is put forward,in wich,the mechanism of chronic sinusitis is blodkade of two orrifices and interties of two toxins.Additionally,on the basis of inducing resuscitation and opening xuanfu,a new treatment principle of dredging sinus orrifi ces,opening xuanfu,removing two toxins and strengthening vital qi are put forward,too.From modern medical point,nose xuanfu is corresponding to intercellular space of nasal mucous membrane,all kinds of ion channels and nasal tissue microcirculation.Nose xuanfu is qi-fluid circulation channel in internal and external environmental material information communication of nasal tissue.Nose xuanfu normal opening-and-closing function keeps normal physiological function of nasal cavity.Nose xuanfu theory provides one new way in rhinology with TCM.

Objective To investigate the relationship between tumor suppressor gene DPC4 and the development and prognosis of pancreatic carcinoma. Methods Relevant literatures of recent years were reviewed. Results DPC4 was located on chromosome 18. Its product was Smad 4 protein. Smad 4 protein was the central component of the transforming growth factor-beta signaling pathway, and all the biological effect was the results of interaction of Smad 4 and different Smads. The gene was deleted or inactive in about 50% of pancreatic carcinomas. The deletion of DPC4 had a great relation to the development and prognosis of pancreatic carcinoma. Conclusion The alteration of tumor suppressor gene DPC4 is connected with the development and prognosis of pancreatic carcinoma. However, this research should be further studied.

Objective To optimize the formulation of self-microemulsifying drug delivery systems containing tanshinone ⅡA(tanshinone ⅡA-SMEDDS) and assess the quality of tanshinone ⅡA-SMEDDS. Methods Pseudoternary phase diagrams were used to choose the oil,emulsifier,co-emulsifier,and their proportion in the formulation on the basis of the ability to form emulsions and regions. Then the self-emulsifying time,droplet and morphology,size distribution,?-potential,stability,and other aspects of the quality were evaluated. Results The formulation of tanshinone ⅡA-SMEDDS was as follows:ethyl oleate-Labrasol-PEG 400=10%:45%:45%,drug loading ratio was 2.25 mg/g. The self-microemulsifying time of tanshinone ⅡA-SMEDDS in 0.1 mol/L hydrochloric acid solution was less than 1 min,the emulsion drops were spherical shape with average particle size of (84.9?2.1) nm and the average ?-potential (-24.0?1.15) mV (n=3). So tanshinone ⅡA-SMEDDS was stable. Conclusion In this paper,the tanshinone ⅡA-SMEDDS has a good self-emulsifying performance. It is expected to further stabilize the preparation of tanshinone ⅡA self-microemulsifying agents.

Objective: To study the prevention and treatment of photosynthetic bacteria (PSB) on atherosclerosis (AS). Method: The hypolipidemic effect of PSB on atherosclerosis (AS) in rabbits induced by high cholesterol diet was studied with Spirulina platensis as positive control. The content of liver TC,AS plaque area percentage (PA) and the emphraxis of coronary artery were determined. Results:PSB could significantly reduce the content of TC in liver, the liver index (LW/BW), and PA 63.99%, 21.96%, 70.10%, respectively, and remarkably abate the lipid aggregation in liver and the pathological changes of coronary artery. In comparison,PSB was better than Spirulina platensis. Conclusion:PSB can prevent the formation of atherosclerosis in hyperlipidemic rabbits.

OBJECTIVE:To compare amiodarone and cedilanid in the treatment of paroxysmal atrial fibrillation(AF) with intravenous administration and control of heart rate.METHODS:47 AF patients were randomly given 150～600 mg amiodarone(amiodarone group,n=24) and 0.2～0.6 mg cedilanid(cedilanid group,n=23) intravenously.The outcome,change of heart rate and adverse drug reaction of 2 groups were observed.RESULTS:Effective rate of amiodarone group was significantly higher than that of cedilanid groups(P

Objective: To study the effect of photosynthetic bacteria (PSB) on serum lipids and antioxidant capacity. Methods: The rabbits were fed high lipid diet to induce hyperlipidemia. The effect of PSB on hyperlipidemia was studied with Spirulina platensis as the positive control. The content of serum TC, TG , HDL-C, SOD activity, and the content of GSH, LPO were determined. Results: PSB could significantly reduce the content of TC,TG , LDL-C in serum, increase H/L and decrease AI ratio. Meanwhile, PSB could improve antioxidant capacity, as greatly increase blood SOD activity, GSH in blood and liver, and reduce serum LPO. In comparison, PSB was better than Spirulina platensis. Conclusion: PSB can significantly regulate lipid metabolism and improve antioxidant capacity in hyperlipidemic rabbits.

Objective To investigate correlation of pulmonary heart disease (PHD)with the diaphragmatic muscle fatigue(DMF). Methods Impedance Pneumoplethysmogram(IPG) and Impedance respirogram(IRG) were detected in 64 cases of patients with PHD,and investigate DMF rheogram positive rate,also analysed various parameters of IPG in positive and negative groups. Results (1)The positive rate of DMF.Rheogram in PHD with and without respiratory failure are 75％ and 21.9％ respectively.(2)The positve rate in PHD with and without heart failue are 80％ and 46.3％ respectively.(3)The mean values of various parameters of IPG in positive and negative DMF rheogram have no significant difference.Conclusion Respiratory failure and heart falure in PHD are closely correlated with DMF. However,the occurance of DMF are not parallel with the severity degree of PHD.

Objective To investigate the technical characteristics and clinical feasibility of 2 implant supported immediate loading mandibular implant complete denture by linear occlusion.Methods40 cases of edentulous patients in the fifth people's hospital of yuhang district hangzhou from January 2011 to January 2017 were selected, these patients were divided into 2 implants supported immediate loading implant supported mandibular complete overdenture group (immediate loading implant group, n=20) and conventional complete denture repair group (n=20) two groups according to the treatment methods, the planting denture satisfactions, body marginal bone resorption, complications of the two groups were statistically analyzed.ResultsThe planting retention function, masticatory function, language function, comfort scores of the immediate loading implant group were significantly higher,there was significant difference between two groups(P<0.05), the bone resorption was significantly lower,there was significant difference between two groups(P<0.05), the incidence of complications 10% (2/20) was significantly lower than the conventional complete denture repair group 40% (8/20).there was significant difference between two groups(P<0.05).ConclusionThe clinical feasibility of 2 implant supported immediate loading mandibular implant complete denture by linear occlusion is higher than conventional complete denture repair.