Delayed cerebral vasospasm (DCVS) is an important incapacitating or lethal cause after subarachnoid hemorrhage (SAH),but the patho-physiological process was vague. The effective drugs of treating DCVS are particularly important including ion channel blockers or openers,endothelin synthetic inhibitor or receptor antagonist,anti-inflammatory agents,antioxidants,fibrinolytic agents,traditional Chinese herbs,etc. The article reviews the research progress in the preclinical and clinical pharmacotherapy on DCVS after SAH,and explores the applications and effects and debates about these therapeutic drugs at present.

Objective To investigate the repair and reconstruction mechanism of stromal cell derived factor-1(SDF-1) on rat abdominal aorta grafts.Methods Male Sprague-Darley rats received abdominal aorta grafts from male Wistar abdominal arteries.Immunohistochemical staining(IHC) was performed to detect the SDF-1 expression.The expression of CXCR4,the receptor of SDF-1,was demonstrated by RT-PCR.(Results) SDF-1 was expressed on the endothelium all the time during the course of the abdominal aorta grafts in the bodies of recipients.Furthermore,it was found that there was a significant relationship between the expression of SDF-1 on the endothelia and the thickness of the neointima of the rat abdominal aorta grafts.On the other hand,CXCR4 was detected within the abdominal aorta grafts.Conclusions Stem cells could be mobilized by SDF-1 to the abdominal aorta grafts and differentiated into smooth-muscle like cells.Furthermore,SDF-1 is predictive of grafts arteriosclerosis and the subsequent development of chronic graft dysfunction(CGD).

The serum manganese, chromium, zinc and copper levels of 63 cases with coronary heart disease and 33 normals were determined by the inductively couple plasma emission spectroscopy. The concentrations of Mn, Cr, Zn and Cu were 0.02145 ?0.0107 ppm, 0.0230 ?0.0167 PPm, 0.905?0.278 ppm 1.056 ?0.404 PPm and the Cu/Zn ratio was 0.973 ?0.395 in normals.In comparing with normals, a significant increase in the serum zinc concentration (P

Intravenous injection of l-stepholildine (SPD) 2.5, 10 and 40 mg/kg decreased blood pressure(BP ) by 30, 40 and 55% respectively in anesthetized dogs. SPD also decreased BP in anesthetized rats (0.5mg/kg iv, by 29% and 2-50 mg/kg injected into duodenum, by 17-36%) and rabbits (2.5 mg/kg iv, by 24%). It could inhibit the pressor reflexes induced by occluding carotid, stimulating vagus Or sciatic nerve. Furthermore, SPD 2.5 mg/kg injected into fourth cerebroventricle of dog decreased BP by 20%, and in pithed rats SPD 10-40 mg/kg iv depreased BP by 7-40%. These results indicate that both central and peripheral mechanisms are involved in the hypotens-ive action of SPD and the peripheral seems to be more important. As showed in previous papers the effect of SPD on ?-adrenoceptors may be one of the chief mechanisms of the hypotensive action induced by SPD.

ObjectiveTo assess the quality of applications for the state funds for natural sciences and the research capabilities of researchers in medicine and other relevant fields in China so as to provide the State Fund Committee for Natural Sciences with scientific basis for systematically planning research funds for the medical field and improving the system of supervising funds for life sciences. MethodsAnalysis was conducted on project applications that were submitted to the Life Sciences Department from 1984 through 2001 and underwent quantitative peer review to explore trends of changes in project applications, the scores and the rate of fund-winning projects. ResultsBoth the quantity and quality of project applications in medicine and other relevant fields showed the trend of going up year by year whereas the rate of fund-winning projects presented an opposite trend. ConclusionThe State Fund Committee for Natural Sciences ought to broaden the scope of subsidization for medicine and other relevant fields and readjust existing funds for life sciences so as to shunt and alleviate the application pressure in medicine and other relevant fields.

Background To explore the roles of human papilloma virus(HPV)and herpes simplex virus (HSV)in pathogenesis of pterygia are very important for provide more basis for the study of pterygia mechanisms.Objective The aim of the study was to detect the expression of HSV and HPV in pterygia. Methods A total of 68 specimens of pterygia and specimens of normal conjunction tissue were obtained during the operation.The expression of HSV DNA and HPV DNA in pterygia specimens and conjunetival tissue were detected and compared by real time fluorescence quantitative polymerase chain reaction(PCR).Written informed consent was obtained from each patient before the any medical procedure related to this study. Results Real time fluorescence quantitative PCR showed that HPV DNA was detected in 12(17.65％)pterygia specimens,and HSV DNA was found in 15 (22.06％)pterygia specimens；however them were detected in 6(8.82％)and 1(1.47％)in conjunctival tissue respectively.Significant differences were found in the expression both HSV DNA and HPV DNA between pterygium tissue and eonjunctival tissue(HPV：χ2=10.291,P<0.05；HSV：χ2=4.561,P<0.05).The co-expression of HSV DNA and HPV DNA was seen in 5(7.35％)pterygium specimens,but no any co-expression of HSV DNA and HPV DNA was in conjunctival specimen. Conclusion HSV and HPV may participate in the pathogenesis and development of pterygium.The detection of virus DNA can offer an experiment evidence of HSV and HPV in pterygium generation.

Cardiovascular System ; Pharmacogenetics

Objective To study the chemical constituents in Lobelia chinensis.Methods Isolation and purification were carried out by silica gel,Sephadex LH-20,and preparative HPLC.Their structures were elucidated by spectroscopy and physicochemical properties. Results Twelve compounds were obtained and identified as: cycloeucalenol(Ⅰ),24-methylenecycloartanol(Ⅱ),phytol(Ⅲ),phytenal(Ⅳ),?-amyrin(Ⅴ),adenosine(Ⅵ),n-butyl-?-Dfructofuranoside(Ⅶ),n-butyl-?-D-fructofuranoside(Ⅷ),n-butyl-?-D-fructopyranoside(Ⅸ),n-butyl-?-D-fructopyranoside(Ⅹ),salicin(Ⅺ),and 5-hydroxymethyl furaldehyde(ⅩⅡ).Conclusion Compounds Ⅰ—Ⅳ and Ⅵ—ⅩⅡ are obtained from the plants of Lobelia L.for the first time and compound Ⅴ is obtained from this plant for the first time.