Objective To explore whether pravastatin protects endothelium against the damage induced by homocysteine (Hcy) in isolated rabbit aorta. Methods Endothelium-dependent relaxation responses to acetylcholine (ACh) of thoracic aortic rings were measured by isometric tension recording before and after aortic rings exposed to Hcy in the absence or presence of pravastatin (PT) to estimate the injury effect of Hcy and the protective effect of pravatatin on rabbit aortic endothelium, respectively. Results Incubation of aortic rings with 1～10mmol/L Hcy for 30min significantly inhibited endothelium-dependent relaxation response to ACh of aortic rings in a concentration-dependent manner. Pre-incubation of aortic rings with 0.3～3mmol/L PT for 15min and co-incubation of aortic rings with 3mmol/L Hcy for another 30min markedly attenuated the inhibition of endothelium-dependent relaxation induced by Hcy in a concentration-dependent manner. Conclusion Pravastatin can improve the impairment of endothelium-dependent relaxation induced by Hcy in isolated rabbit aorta.

AIM: To explore the effects in different dosage of ketamine on the expression of nitric oxide synthase (NOS) in medial septal nuclei (MS) and vertical diagonal band (VDB) of rats. METHODS: 40 SD rats were randomized into normal control group,normal saline(NS) group, low, middle and high dosage of ketamine(25,50,100 mg?kg~ -1 ,ip) group.Each group was maintained intraperitoneally by given daily dosage once a day for a period up to 7 days. That was done at 2100 hour each night, and by 2 h of the last administration, poured, fixed and sliced were carried out. Method of nicotinamide adenosine dinucleotide phosphate diaphorase (NADPH-d) was used to show the expression of NOS in MS and VDB pars dorsalis and ventro. RESULTS: Compared with normal control and NS group, the number of NOS positive neurons were decreased in MS and VDB pars dorsalis and ventro in ketamine groups.There were statistical meaing in different-dosage groups of ketamine(P

Objective To investigate the expression level of Annexin Ⅱ in bronchial lung cancer tissue,alveolar lavage fluid(BALF) and pleural effusion and its value in the diagnosis of lung cancer.Methods From January 2014 to March 2016 in Jingzhou Infectious Disease Hospital,80 cases of patients with pleural effusion of lung cancer (Lung Cancer Group),40 patients with benign pleural effusion (benign group) were chose.The expression level of Annexin of two groups of patients with pleural effusion,BALF were detected and compared.Sixty specimens of department of pathology past (after lung cancer surgery cancer) and 30 cases of previously collected normal lung tissues (normal lung tissue) were collected,differential expression by immunohistochemical staining were compared between the two groups of Annexin Ⅱ protein.Results The positive expression of Annexin Ⅱ in lung cancer was 61.67％ (37/60),higher than that in normal lung tissue of 23.33％ (7/30),the difference was statistically significant(P＜0.001).The positive expression of Annexin Ⅱ in lung cancer patients was significantly correlated with rate of lymph node metastasis and clinical stage of lung cancer(P =0.039,0.029).Group of patients with pleural effusion,BALF expression of Annexin Ⅱ the level was higher than that in benign group(P＜0.001).When the critical value of Annexin in pleural effusion of lung cancer diagnosis was 10.94 μg/L,the sensitivity of diagnosis of lung cancer was 83.47％,the specificity was 80.66％,AUC value was 0.837.The critical value when BALF Annexin Ⅱ in the diagnosis of lung cancer was 52.08 μg/L,the sensitivity of diagnosis of lung cancer was 86.19％,the specificity was 89.22％,the AUC value was 0.898.Conclusion The expression level of Annexin Ⅱ in the BALF and pleural effusion of patients with bronchial lung cancer and lung cancer is increased,and it has a certain clinical value for the differential diagnosis of lung cancer.

Insulin has been found to enhance specific immune function in experimental animals. However, it remains hitherto unnoticed whether the antigen-presenting capability and expression of Ia antigens of the macrophage, known to be essential for the induction of both cell-mediated immunity and humoral immune response to most antigens, could also be influenced by insulin. In the present study, it was demonstrated that insulin, in a concentration of 1.03?10~(-7) mol/L, promoted the antigen-presenting capability of human monocytes significantly. Furthermore, we found that the expression of HLADQ antigen on monocytes was markedly enhanced by insulin while that of HLA-DR determinant was not affected significantly. We suggested, therefore, that the promotion of antigen-presenting capability of human monocytes by insulin may be attributed, at least in part, to its enhancing effect on the expression of HLA-DQ antigen on the monocytes.

Zishengwan is one of the representative prescription of invigorating spleen and stomach,which is widely used in different clinic departments.This article introduces Zishengwan being used in treating different diseases in the past few years,aiming at certifying that different diseases can be treated with same method,providing the basis of expanding clinical applied range of Zishengwan.

Objective To explore the causes and treatment of gastrointestinal complications after anterior approach for thoracolumbar fracture and dislocation.Methods A retrospective analysis was carried out in 153 cases with thoracolumbar fracture and dislocation undergone anterior approach from Jan- uary 1999 to October 2003.Postoperative gastrointestinal complication was seen in 15 cases including sev- en with dynamic intestinal obstructions,three with stress ulcer,three with intestinal bacteria imbalance, one with tuberculosis peritonitis resulted from dissemination of primary tuberculosis,and one with acute relapse of chronic appendicitis.Results All patients were effectively cured by using corresponding methods in regard of different causes.Conclusions(1)Gastrointestinal complications following ante- rior approach for thoracolumbar fracture and dislocation are mainly resulted from following causes,ie,se- rious primary trauma,primary gastrointestinal vegetative nerve injury or that caused by surgical operation, intraoperative contusions of abdominal viscera,postoperative retroperitoneal hematoma,acute lesion of gastric mucous membrane as well as imbalance of intestinal flora.(2)Correct treatment of primary trau- ma,meticulous operation,reasonable utility of antibiotics and appropriate management can effectively prevent and control gastrointestinal complications.

The main function of interstitial cells of Cajal(ICCs) is to produce the gastrointestinal slow-wave electric potential,to impel the propagation of electric potential,and to participate in the transmission of neural signal.The decrease or abnormal distribution of ICCs would lead to gastrointestinal inflammation easily.Meanwhile,as the gastrointestinal inflammation happens,inflammatory cells and mediators also can reduce the number of ICCs.

Airway problems in foetus that may cause airway obstruction can be safely managed by using an ex utero intrapartum technique(EXIT).The EXIT procedure maintains a period of uteroplacental circulation for fetal oxygenation during delivery to provide time to explore and secure the airway of the fetus or to perform fetal operations before the umbilical cord is clamped.The common indication for the EXIT procedure is the presence of foetal airway obstruction,which is usually caused by neck mass,congenital diaphragmatic hernia,congenital high way obstruction syndrome,thoracic abnormality,congenital heart and lung dysfunction,conjoined twins,and so on.It avoids the acute airway obstruction in a neonate that has disastrous consequences.

Objective To observe the effect of granulocyte colony-stimulating factor carried by fibrin gel on the total number of peripheral white blood cells and neovascularization of ischemic muscle in rat hindlimb ischemia model.Methods Thirty male SD rats were subjected to right hindlimb ischemia and randomly divided into three groups:Gel + G-CSF,G-CSF,and PBS,respectively injected with Gel+G-CSF,G-CSF and PBS.WBCs was detected before and 1,3,5,7 days; At first week and fourth week after surgery,5 rats in each group were sacrificed,and after histological detections was performed,blood vessels density counted.Results The peak value of WBCs counts in group Gel + G-CSF (14.69 ± 1.11 × 109/L)appeared at postoperative day 1 was significantly lower than that in group G-CSF (21.00 ±2.26 × 109/L) at postoperative day 3.The capillary density in group Gel + G-CSF (686 ± 108/mm2) was significantly higher than that in group G-CSF (491 ± 110/mm2) and group PBS (252 ± 78/mm2),P ＜ 0.05.The α-SMA-positive blood vessel density in group Gel + G-CSF (6.1 ± 0.8/mm2) was significantly higher than that in group PBS (2.6 ± 1.3/mm2),P ＜ 0.05.In group Gel + G-CSF,there were many VEGF-positive cells infiltrating in ischemic limb.Conclusions Gel + G-CSF promotes neovascularization in ischemic muscle,and induced more modest WBCs counts increase than the treatment with G-CSF.

OBJECTIVE To investigate the present situation of hand-washing among medical staff then explore more suitable hand-washing methods and monitoring measure. METHODS By surveying the hand-washing situation of 96 medical staff in our hospital through the method of sampling,the different germ eradication rate between routine soap hand-washing and disinfectant wet towel hand-wiping as well as hand-washing rate in all candidate population during practical work were observed. RESULTS The germ eradication rate after routine soap hand-washing was 86.0%,while by disinfectant wet towel wiping it was increased to 91.3%.The washing rate after above former method was 18.3% and after above two methods combined was increased to 43.6%,and after health propaganding and training was up to 60.6%. CONCLUSIONS Disinfectant wet towel hand-wiping is convenient,practical and effective which would be more suitable for clinical practice when combined with routine soap washing method.