Objective To observe the stability and the change pattern of VEGF expression in myocardium in varied postmortem periods,and to evaluate its usefulness in diagnosis of early myocardial ischemia after death.Methods An animal model of early myocardial ischemia was established by ligating coronary artery of rabbit,immunohistochemistry(SP protocol),image analysis technique and statistical analysis system are applied to detect and compare the area and intensity of expression of VEGF in cardiocytes,and its stability in different postmortem perieds.ResultsIn ischemic myocardium,VEGF shows foci or sheets of intense positive staining in cardiocytes,with negative or weak staining in some cells.There was weak staining in vascular endothelial cells and smooth muscle cells,but no staining in interstitium.When ischemic myocardium stored at 4℃,the staining of VEGF became weaker with time delay,but there was weak staining in a fraction of cardiocytes even 10d after death.There was significant difference in the intensity of VEGF staining between ischemic and normal postmortem myocardium from 1 to 10d.Conclusion VEGF can withstand a certain degree of autolysis or putrefaction,and the immunohistochemical staining of it is valuable in the diagnosis of early myocardial ischemia in corpses stored at 4℃ for 1~10d after death.

Objective To observe curative effect of mental therapy on women perimenopausal period syndrome.Methods 100 patients with perimenopausal period syndrome were randomly divided into the treatment group and control group with 50 cases in each group. Patients of the treatment group were treated by Cryzanolam, Surazepam, etc,and mental therapy was given simultaneously twice one week. Patients of the control group were treated only by drugs. The period of treatment was four weeks. The curative effect was assessed by SCL-90 symptom self evaluation scales.Results Several factors and total average points of two groups' SCL-90 all dropped, and there was significant difference between two groups after therapy (P<0.01). For patients of the treatment group, clinic symptoms were markedly abated after treatment (P<0.05), and average scores of several factors were not significantly different from normal adults (P>0.05). Conclusion The mental therapy can remit symptoms of women perimenopausal period syndrome effectively and improve their healthy.

Objective To study the correlations between diopter,axis length,cornea curvature and thickness in myopia patients. Design Prospective case series.Participant 300 myopic patients who were ready for LASIK.Method 300 myopic patients who were ready for LASIK were selected randomly.They were divided into four groups according to the diopter of myopia:low myopia group (≤-3.00D)41 cases,medium myopia group(＞-3.00D,≤-6.00D)130 cases,high myopia group(＞-6.00D,≤-9.00D)95 cases,and super myopia group(＞-9.00D)34 cases.The axis length,cornea thickness,cornea eurvture and diopter of myopia were detected. Through analysis of variance(ANOVA),Pearson correlation analysis and linear regression analysis,the correlations between axis length, cornea thickness,curvature and diopter of myopia were made out in four groups.Main Outcome Measure Diopter of myopia,cornea curvature,axis length of eye and central cornea thickness.Result The axis length of low myopia group,medium myopia group,high myopia group and super myopia group was(24.32?1.73)mm,(25.33?1.49)mm,(26.04?1.92)mm,(27.47?2.57)mm respectively. There were obvious differences of axis lengths between four groups(P=0.000).The cornea curvature in high myopia group was obviously different from those in low myopia group and medium myopia group.There were no significant differences of cornea thickness among four groups(P=0.409).The axis lengths of eyes respectively had correlations with diopters(r=0.734,P=0.000),vertical and horizontal cornea curvatures(r=-0.312,P=0.000;r=-0.328,P=0.000).Conclusion The main effective factor on the diopter of myopia is the axis length for most patients.For the patients with high myopia,the cornea curvature is another effective factor.With the increase of axis length,the cornea curvature decreases,and the cornea turns flatter.(Ophthalmol CHN,2006,15:321-323)

Objective To observe the effect of ephedrine on the expression of eotaxin in human bronchial epithelial cells (16HBE) stimulated by tumor necrosis factor‐α(TNF‐α) and to explore the mechanism of Chinese medicine ephedra in treating asthma .Methods The in vitro cultured 16HBE were randomly divided into the control group ,TNF‐αstimulation group(TNF‐α20 ng/mL) and TNF‐αplus ephedrine group (TNF‐α20 ng/mL plus ephedrine 300 μg/mL) .Three complex holes in each group were set to culture for 18 h ,the eotaxin mRNA expression was measured by real time fluorescent quantified PCR and protein level was detected by immunocytochemical stain and Western blot .The eotaxin concentration in cells culture supernatant was quantified by ELISA .Results Compared with the the control group ,the expression level of eotaxin mRNA and protein ,and the concentration of eotaxin in cell culture supernatant in the TNF‐α stimulation group were increased obviously ,there being statisticaly significant difference between them(P<0 .01);however ,all above these parameters in the TNF‐αplus ephedrine group showed decreased obvi‐ously as compared with the TNF‐αgroup ,the difference between them was statistically significant (P<0 .01) .Conclusion Ephed‐rine can inhibit the expression and secretion of eotaxin in TNF‐α induced 16HBE inflammatory model ,which may be one of the mechanisms of Chinese medicine ephedra in treating asthma .

Objective: To explore the living standard of young college teachers engaged in psychological counseling in Hunan province.Methods: 44 young college teachers engaged in psychological counseling and 52 control subjects were tested by the generic quality of life inventory-74(GQOLI-74).Results: ① The scores of the living standard were noticeablly different between the young college teachers engaged in psychological counseling and other young college teachers,the scores of the former’s living standard were markedly lower than those of the latter’s.②The total score,scores of factors such as physical distress、 movement and sense function、 overall comments on the living standard,and score of the material life dimension of GQOLI-74 of male young college teachers engaged in psychological counseling were evidently higher than those of female young college teachers’ engaged in psychological counseling.③19 factors and sex which probably influence the overall comments on the living standard were selected regression equation.Conclusion: The young college teachers engaged in psychological counseling have poor life quality and subjective well-being.

Objective To explore the diagnostic value of SPECT rCBF imaging for acute closed brain injury. Methods SPECT rCBF imaging and X-ray CT were perfomed in 60 patients with acute closed brain injury, and the results of two detection methods were compared. Results Among 60 patients, SPECT examination found 54 cases(90%) of brain lesions, while x-ray CT only found 27 cases(45%). The positive diagnostic rate of SPECT was higher than that of CT(? 2=25.68,P

Objective: To determine the effects of intravenous anesthetics (fentanyl,midazolam and propofol )on inflammatory responses. Method: We employed carrageenan-sensitized endotoxemic rabbit model. Thirty-two New Zealand rabbits were randomly assigned to one of four groups,control group(no anesthetic was used), fentanyl group, midazolam group and propofol group. Different anesthetics were used intravenously for anesthesia induction and maintenance according to the group classification. Meanwhile, all animals were mechanically ventilated, then, 2?g?kg~(-1) lipopolysaccharide(LPS) were injected for endotoxin challenge. Result:In all groups, serum tumor necrosis factor (TNF)activity began to increase at 30 min,reached its maximal level at 60 min,and tended to decrease at 120 min after (LPS) challenge,and the significant increase of TNF activity was associated with development of hypotension. Meanwhile,serum phospholipase A_2(PLA_2)activity began to increase at 60 min and persisted in going up within 2h. Those animals,with continuous infusion of these three different anesthetics and mechanical ventilation,had much lower peak level of TNF and higher mean arterial pressure (MAP)than control levels, but their changes of serum PLA_2 activity had no significant difference from control level. Conclusion: With mechanical ventilation, intravenous infusion of these anesthetics can reduce LPS induced injury in the acute phase of endotoxemia, probably by the inhibition of TNF production.

Objective To study the chemical constituents in Lobelia chinensis.Methods Isolation and purification were carried out by silica gel,Sephadex LH-20,and preparative HPLC.Their structures were elucidated by spectroscopy and physicochemical properties. Results Twelve compounds were obtained and identified as: cycloeucalenol(Ⅰ),24-methylenecycloartanol(Ⅱ),phytol(Ⅲ),phytenal(Ⅳ),?-amyrin(Ⅴ),adenosine(Ⅵ),n-butyl-?-Dfructofuranoside(Ⅶ),n-butyl-?-D-fructofuranoside(Ⅷ),n-butyl-?-D-fructopyranoside(Ⅸ),n-butyl-?-D-fructopyranoside(Ⅹ),salicin(Ⅺ),and 5-hydroxymethyl furaldehyde(ⅩⅡ).Conclusion Compounds Ⅰ—Ⅳ and Ⅵ—ⅩⅡ are obtained from the plants of Lobelia L.for the first time and compound Ⅴ is obtained from this plant for the first time.

Objective To investigate the genetic susceptibility of HLA DRB1, DQB1 alleles to colorectal neoplasm in Hubei Han Chinese. Methods 54 patients with colorectal neoplasm and 136 healthy controls were examined for HLA DRB1 and HLA DQB1 genotypes, which were typed by using polymerase chain reaction/sequence specific primer (PCR/SSP) technique and allele sequence analysis.SAS(6.12 for win) was used for statistics. Results Compared with the control, the allele frequency (AF) of HLA DRB1*0901 (0.2315 vs 0.1397, P =0.033) and DRB1*080X(0.009 3 vs 0.080 9, P =7.11?10 -3 ) was significantly increased or decreased, respectively, in colorectal neoplasm of Hubei Han Chinese. And there was not significantly different between the patients with the control in the AF of HLA DQB1 alleles. Conclusion The results indicate that HLA DRB1*0901 allele is the positive association to , but DRB1*080X is the negative association to the patients of colorectal neoplasm among Hubei Han Chinese. The nucleotide sequences of these two alleles were identified by allele sequence analysis, approach to the corresponded allele sequence(exon 2)of genbank.There is not association HLA DQB1 alleles with the patients.

Objective To evaluate the biocompatibility of nano-hydroxyapatite/polyvinyl alcohol (n-HA/PVA ) hydrogel com-posite for keratoprosthesis .Methods There were four materials to be evaluated ,big-porous and small-porous n-HA/PVA hydrogel composite ,big-porous and small-porous PVA hydrogel .Every kind of materials was digested in F12 nutrient culture for 24 h to get the materials impregnant .Corneal fibroblasts were cultivated in materials impregnant and through methyl thiazolyl tetrazolium col-orimetry to assay the cytotoxicity .Rabbit corneal fibroblasts were seeded onto the four materials in vitro .Three days later the growth of fibroblasts were observed by inverted microscope and scanning electron microscope (SEM ) .Results The cytotoxicity scores of the materials with big-porous were both grade one .The mean corneal fibroblasts counts on culture were no obvious differ-ence between big-porous materials groups and control group under plate inverted microscope 72 hours later(P=0 .608) .Through scanning electron microscope 72 hours later ,corneal fibroblasts grew well on n-HA/PVA hydrogel composite ,and migrated into the porous of composite materials .The corneal fibroblasts on big-porous n-HA/PVA hydrogel composite were more obviously found than the other three materials(F=95 .245 ,P=0 .000) .Conclusion The porous n-HA/PVA hydrogel composite is a kind of good biocompatible material which has no cytotoxicity .The n-HA/PVA hydrogel composite with big porous would be an ideal pe-ripheral skirt of keratoprosthesis .