Objective To study the changes of sodium currents(I_(Na))of ventricular cells in rats after acute pancreatitis.Methods Rats'models of acute panereatitis were produced by injecting sodium taurocholate into the pancreatic duet.After 24 hours,single ventrieular cells was isolated enzymatieally,and I_(Na),were recorded by using patch clamp techniques.Results I_(Na) peak from ventricular cells in the acute panereatitis group was significantly reduced(- 6.80?2.03)pA/pF compared with that in control group(-13.55?5.33)pA/pF,P＜0.001.The steady-state inactivation curve was shifted to upward direction,the half-maximal voltage dependence of inactivation(V_(0.5))was(-121?26)mV in ventrieular cells from panereatitis group and(-105?21)mV form the control group.I_(Na) returned to normal more slowly in ventrieular cells from panereatitis group than that from control group.Conclusion Inhibitation of I_(Na) was found in ventrieular cells,which might cause arrhythmias in rats with acute pancreatitis.

BACKGROUND:The measure and prediction of the shape of optic nerve head are the key issues for early diagnosis and duration prediction of glaucoma. Therefore, it is significant to research the shape variation of optic nerve head under high intraocular pressure.
OBJECTIVE:To establish three-dimensional finite element model of optic nerve head and to analyze the deformation of optic nerve head and the change in retina thickness after acute high intraocular pressure.
METHODS:Animal model of acute ocular hypertension was established by methods of anterior chamber perfusion. The tomographic images of the optic nerve head of a cat were obtained at the normal intraocular pressure and 5 320 Pa, 7 980 Pa, 10 640 Pa, 13 300 Pa, 15 960 Pa intraocular pressures using optical coherence tomography. Then, we measured the variation of retinal thickness at typical location. Basing on the tomographic images of optic nerve head of a cat at normal intraocular pressure, we obtained three-dimensional structure of retina and choroid using software MIMICS. Then three-dimensional model of optic nerve head was established by assembling the retina and choroid. The deformation of optic nerve head and the change in retinal thickness under high intraocular pressure were observed using software ABAQUS. The effectiveness of the model was verified by the experimental result.
RESULTS AND CONCLUSION:With the increase of intraocular pressure, the retinal thickness is thinner, the optic nerve head depth, width and the ratio of the depth to width are gradual y increased. It suggests that the acute high intraocular pressure causes retinal thinning, optic nerve head widening and deepening. It is feasible to establish optic nerve head modeling in vivo by using optical coherence tomography, mechanical analysis can be applied to predict the shape variation of optic nerve head, which is significant to further deduce the pathological process of glaucoma.

Objective To measure and investigate the anatomical characteristics of the proximal femur canal curves in patients with developmental dysplasia of the hip (DDH), and to provide references for the design of femoral stem of DDH patients. Methods Three-dimensional reconstructions of the proximal femur were conducted from the CT data, and the anatomical morphology characteristics were obtained from 64 patients with DDH (74 hips, 12 males, 52 females, average age of 45.3 years) and 30 healthy controls (30 hips, 21 males, 9 females, average age of 29.4 years). Multiple anteroposterior diameter and mediolateral diameter around the lesser trochanter at different heights and the fitting curves of different groups were obtained. Results The femoral neck anteversion angle, neck-shaft angle, height of the isthmus, canal flare index (CFI), femoral head diameter and femoral offset of DDH patients and healthy controls were 26.39°±14.74° and 15.68°±7.95°, 125.65°±5.73° and 129.19°±5.80°, (99.14±14.62) mm and (110.13±11.73) mm, 3.63±0.77 and 4.45±0.79, (44.01±5.75) mm and (47.26±3.94) mm, (31.80±3.82) mm and (36.42±4.84) mm, respectively. DDH group had the significantly greater femoral neck anteversion angle, and other anatomical morphology characteristics were significantly smaller than the control group. The obtained anteroposterior diameter, mediolateral diameter and the fitting curves showed that DDH group had narrow medullary cavity and forward shifting anteroposterior diameter. In addition, there was a statistical significance between the Crowe Ⅳ DDH and other types of DDH, and deformation degree of the femur increased with the increase of DDH type. Conclusions The proximal femur of DDH patients was significantly different from the healthy controls. Moreover, there were significantly morphological differences between different types of DDH. The research findings can be used as quantitative references for understanding the morphological feature of DDH patients and the corresponding design of femoral stem for DDH patients.

Objective Aiming at solving the problems of pain on the anteromedial tibia, tibial component loosening and osteoarthritis progression after unicompartmental knee arthroplasty (UKA), the influence of different geometric shapes of tibial component pegs on stress distributions in tibia was analyzed by finite element method. Methods The finite element models with UKA were established and validated. Geometric shapes of tibial component were designed. Under the same loading condition, the tibial components with double-peg, single-keel, double-keel and cross-star were studied for finite element analysis and compared with intact model, so as to evaluate the influence of tibial component with different shapes on stresses of cortical bone in anteromedial tibia, cancellous bone under tibial component and cartilage in contralateral tibia. Results Compared with the intact model, the peak stress of cortical bone in anteromedial tibia with double-peg, single-keel, double-keel and cross-star tibial components increased by 56.1%, 55.9%, 54.5% and 68-4%, respectively. The peak stress of cancellous bone under tibial component with single-keel and double-keels decreased by 8.1% and 15.6% respectively, while the peak stress of cancellous bone under tibial component with double-peg and cross-star increased by 67-9% and 121-5%, which were higher than the fatigue yield stress of cancellous bone. The peak stress of cartilage in contralateral tibia with double-peg, single-keel, double-keel and cross-star tibial components decreased by 42.1%, 26.6%, 24.2% and 28.5%, respectively. ConclusionsThe load distribution of the medial and lateral tibia changed after UKA operation, and a greater load was observed on the replacement side. Single-keel and double-keel tibial components were more effective in reducing stresses on cortical bone in anteromedial tibia and cancellous bone, while the stress distribution in tibia with single-keel tibial component was closer to that of the intact tibia. The research findings can provide theoretical references for designing single-keel tibial component of unicompartmental knee prosthesis which conforms to better mechanical properties of the knee joint.

Objective To compare biomechanical properties between the anatomical clavicle plate and the reconstructed clavicle plate, and investigate the influence of pre-bending or repeated bending process on biomechanical properties of the reconstructed clavicle plate, so as to provide biomechanical evidence for treating midshaft clavicle fracture in clinic. Methods The reconstructed clavicle plate was bent by 1, 2, 3, 5 times respectively based on shape of the anatomical clavicle plate. The biomechanical differences in anatomical plate group, reconstructed plate group and pre-bending plate group were compared by static compression test. Results The fixation stiffness and strength of the anatomical plate were better than those of the reconstructed plate. There was no significant difference in stiffness and strength between the anatomical plate group and one-time bending group. Pre-bending had a great effect on mechanical properties of the clavicle plate, and stiffness and strength of the clavicle plate were obviously improved after one-time bending. The mechanical properties of the clavicle plate were obviously reduced by bending of the clavicle plate after one-time bending. Conclusions The stiffness and strength of the anatomical plate and one-time bending plate are higher than those of the normal reconstructed plate. It is recommended that surgeons should perform bending appropriately according to the patient’s clavicle anatomy when using the reconstructed clavicle plate for fracture fixation, and minimize the number of repeated bending to maintain stiffness and strength of the clavicle plate.